Gene

KWMTBOMO03495

Pre Gene Modal

BGIBMGA006347

Annotation

PREDICTED:_DNA_helicase_MCM9-like_isoform_X1_[Amyelois_transitella]

Location

Bomo_Chr6(-):9663886-9674049      View in EpiBrowser

Full name

DNA helicase MCM9      

Alternative Name

Mini-chromosome maintenance deficient domain-containing protein 1
Minichromosome maintenance 9

Location in the cell

Nuclear   Reliability : 3.974

CDS

ATGATTTTAGAATATATATTAAAGTTCCATTTGGATGATTGTTTTAATGTATTATCAGAAAAAGATAATCTAGGATATTTTAGCATTAAAATCGATTTTTTAAAATTATTTGAAAACCATCCTGATGTGGGAGACAGAGTTCTGTGTGCTCCCATTGAAACCCTGCCCATATGCAATAAAGATATAGTGATGGCCCAACAACACATTATAGAATCTGAGGAATTTAAAAATACAGTTTCGAAATTAGAATATGTTTTCGTAAAGAAGAATGTTCATGCCAGATTTTATGGTTTACCTGTTTGTCCGGAGTTACACAGAACAGTTTTCCCTAAAAATGTAGATCTGGGATGTTTTTTAAAAGTAACAGGTACAGTTGTCCGAGTGACCCAGTCCAAAATGTTGGAATATCAAAGAAAGTATGTCTGTATGAAATGCAAATATGAGAATTATGTTCAAGCAGATTTCGAAAGGAGATACATTCTTAAATCACCATCAAAATGTTGTAATATTGATCCAAAATGCAGAAGTACAATATTCTCACAAGTGAATCTTGTATCCAGAGAATATTGTAAAGATTATCAGGAAATAAAAATTCAGGAGCAAGTTAACAAGCTTAGCATTGGTACCATTCCAGGCTCATTCTGGGTGGTTTTAGAAGATGATTTAGTAGATTGTTGTAAACCTGGTGATGATGTTATAATTTGCGGTACTATTAGAAGACGTTGGCGACCATCATTTCAAACAAAAAAATCTGAAGTGGAACTAGTCCTCCAAGCAAATTACATAGAAGTGTGTAATGCGCTAAGATCTAAAGTGGTAGCCACAGCTCCTGACATAAAAGAATGCTTTGACAGTTTCTGGTCTTACTATGATTCGTGTCCTTTGAAAGGCAGAGATCATATACTTAGATCGATTTGTCCTCAAGTCTACGGTCTACATTTGGTAAAGTTGGCTGTGTTGCTCACGGTGATTACCGGATCGAATCACATAGTCGAGGAAACAGATGAAAAGAATACATCCTTTGACGATAATCATCATACAAAAGTGCGGGGTCAGTGTCATCTCCTGTTAGTTGGAGATCCAGGCACTGGGAAGTCGCAACTGCTTCGTTCAGGAGCAGATCTGACTTCGCGTTCGGTGTTCACTTCGGGAGCTGGCAGTACGCGAGCCGGCCTCACGTGTGCTGCGCTCAGGGAAGACGGCGAGTGGCAGCTAGAAGCTGGAGCACTGGTTCTGTCGGACGGCGGCGTGTGCTGTATAGATGAGATCTCCCAGCTGAAGGAGCACGACCGTACCGCCATACACGAGGCGATGGAGCAACAGACAATCTCCATAGCCAAGGCAGGTATAGTCTGCAAATTGAACACACGTTGTGCTGTGATAGCAGCCTGCAATCCTAAGGGGAACTACGATTCAAATCAACCGCTCAGTATGAATTTAGCACTTGGAACGCCACTTTTGAGCCGATTTGATCTAATTTTCATTCTATTGGATACAAAAAATAAAGCTTGGGATAAACTTGTATCTTCTTATATATTGTTCGGTGATTCAAATTTAGAACAAAATAAAAAATATTGGAATCTCGAAAAATTGCAGATGTACATTAGTCTAGTGGGACCAAGGACGACAAAGATGACAAAATCGGCAAACATTATCCTGCAATCGTATTATATGGCCCAGAGAAAAGCCGAAAGCAGAGATCCATCACGTACTACCGTGAGAATGCTCGACAGTCTGGTCAGACTATCACAAGCTCACTGCCGTTTGATGTATAGAACAACAATATATCCGATGGACGCCATTATAGCCGTATCTTTAGTAGATTTATCAATGCAAGACTGCACTTTATCAGACACTGCGGATGCGCTACATTCCAATTTCTATAAATACCCTGACTTTGAATACCTATGCACAGCAAAAAAACTATTAACAAAATTAAATTTGTACGAAATATGGCGAAATGAATTATTACACTACGGTAAATTACTTCAGGTCGATCACAAAACATTGGAACATGACATTGATAGCGGAATCATTAAGCTTTTCGCGAAATATGATGATGTCGCCGATGAAAATACACCTTTGTCAGCATCGGTAATAACAAGTTCTTATTTTAAGAAAGAAGAAAATGGATTGTTACATAATGACAAAAACAGTTTACCCGATGAAGAGAACAAAGATATCATAGAAGTAAATAAAATGTTGGCAGTGACATTGAAAAAACACGCAACTTTAAATAGAGACGAAAGAAAACTACCAGTAGCTCAGAAAAAACCTCAAAATAAAAGAAAACGGAAAGAAGTTACTGTGAATTCAAAAATTCTTAAAAGTTTAGTTCCTAAAAAGCGTAAGAAATCAAAGGAGGAAGTAAGGGATGCCAGTAGTCCTACAAACATTGACGTTGACCAGAATCATCTCTTAAATGCAGTGCCTAGTGTTAATGATGTGTTTGCAGAGTACGGTATCAATCTTAAGTTTGAAGGTGAAAGTCAACATAAATCAAGTGGTAAAAGAATCGAATTAAAAGAAAATAAGATTGATAATGAAATCATTGTTCCTAGAATTGAAAAAAAGGATCTTGTAGAAAACAAAACTAATTCTCATAAAGAACAAAATGCAAATATCTCAACAATAAATAAATTAAAACAGTTCGCATGTGAACAAAAATATAACATTGATAAATATGAAGAAAAGTTATCGATATCTGAATACAACAACAGTTTTAAATTTAATATTGAGAGTAAAAGTACAAATGCAGTTCAAGATAAGTCTGAACGAAGTTCCCAAATATCAATTTTTGAAAGTTCAGATTGTGATATTGATTTAGATTTATGA

Protein

MILEYILKFHLDDCFNVLSEKDNLGYFSIKIDFLKLFENHPDVGDRVLCAPIETLPICNKDIVMAQQHIIESEEFKNTVSKLEYVFVKKNVHARFYGLPVCPELHRTVFPKNVDLGCFLKVTGTVVRVTQSKMLEYQRKYVCMKCKYENYVQADFERRYILKSPSKCCNIDPKCRSTIFSQVNLVSREYCKDYQEIKIQEQVNKLSIGTIPGSFWVVLEDDLVDCCKPGDDVIICGTIRRRWRPSFQTKKSEVELVLQANYIEVCNALRSKVVATAPDIKECFDSFWSYYDSCPLKGRDHILRSICPQVYGLHLVKLAVLLTVITGSNHIVEETDEKNTSFDDNHHTKVRGQCHLLLVGDPGTGKSQLLRSGADLTSRSVFTSGAGSTRAGLTCAALREDGEWQLEAGALVLSDGGVCCIDEISQLKEHDRTAIHEAMEQQTISIAKAGIVCKLNTRCAVIAACNPKGNYDSNQPLSMNLALGTPLLSRFDLIFILLDTKNKAWDKLVSSYILFGDSNLEQNKKYWNLEKLQMYISLVGPRTTKMTKSANIILQSYYMAQRKAESRDPSRTTVRMLDSLVRLSQAHCRLMYRTTIYPMDAIIAVSLVDLSMQDCTLSDTADALHSNFYKYPDFEYLCTAKKLLTKLNLYEIWRNELLHYGKLLQVDHKTLEHDIDSGIIKLFAKYDDVADENTPLSASVITSSYFKKEENGLLHNDKNSLPDEENKDIIEVNKMLAVTLKKHATLNRDERKLPVAQKKPQNKRKRKEVTVNSKILKSLVPKKRKKSKEEVRDASSPTNIDVDQNHLLNAVPSVNDVFAEYGINLKFEGESQHKSSGKRIELKENKIDNEIIVPRIEKKDLVENKTNSHKEQNANISTINKLKQFACEQKYNIDKYEEKLSISEYNNSFKFNIESKSTNAVQDKSERSSQISIFESSDCDIDLDL

Description

Component of the MCM8-MCM9 complex, a complex involved in the repair of double-stranded DNA breaks (DBSs) and DNA interstrand cross-links (ICLs) by homologous recombination (HR) (PubMed:23401855, PubMed:22771120). Required for DNA resection by the MRE11-RAD50-NBN/NBS1 (MRN) complex at double-stranded DNA breaks to generate ssDNA by recruiting the MRN complex to the repair site and by promoting the complex nuclease activity (By similarity). Probably by regulating the localization of the MNR complex, indirectly regulates the recruitment of downstream effector RAD51 to DNA damage sites including DBSs and ICLs (PubMed:22771120, PubMed:23401855). Acts as a helicase in DNA mismatch repair (MMR) following DNA replication errors to unwind the mismatch containing DNA strand (PubMed:22771120, PubMed:26300262). In addition, recruits MLH1, a component of the MMR complex, to chromatin (By similarity). The MCM8-MCM9 complex is dispensable for DNA replication and S phase progression (PubMed:21987787). Probably by regulating HR, plays a key role during gametogenesis (PubMed:21987787, PubMed:22771120).
Component of the MCM8-MCM9 complex, a complex involved in the repair of double-stranded DNA breaks (DBSs) and DNA interstrand cross-links (ICLs) by homologous recombination (HR) (PubMed:23401855). Required for DNA resection by the MRE11-RAD50-NBN/NBS1 (MRN) complex by recruiting the MRN complex to the repair site and by promoting the complex nuclease activity (PubMed:26215093). Probably by regulating the localization of the MRN complex, indirectly regulates the recruitment of downstream effector RAD51 to DNA damage sites including DBSs and ICLs (PubMed:23401855). Acts as a helicase in DNA mismatch repair (MMR) following DNA replication errors to unwind the mismatch containing DNA strand (PubMed:26300262). In addition, recruits MLH1, a component of the MMR complex, to chromatin (PubMed:26300262). The MCM8-MCM9 complex is dispensable for DNA replication and S phase progression (PubMed:23401855). Probably by regulating HR, plays a key role during gametogenesis (By similarity).
Component of the MCM8-MCM9 complex, a complex involved in homologous recombination repair following DNA interstrand cross-links and plays a key role during gametogenesis. The MCM8-MCM9 complex probably acts as a hexameric helicase required to process aberrant forks into homologous recombination substrates and to orchestrate homologous recombination with resection, fork stabilization and fork restart. In eggs, required for MCM2-7 loading onto chromatin during DNA replication. Probably not required for DNA replication in other cells (By similarity).

Catalytic Activity

ATP + H2O = ADP + H(+) + phosphate

Subunit

Component of the MCM8-MCM9 complex, which forms a hexamer composed of MCM8 and MCM9 (PubMed:22771120). Interacts with the DNA mismatch repair (MMR) complex composed at least of MSH2, MSH3, MSH6, PMS1 and MLH1 (By similarity). Interacts with MLH1; the interaction recruits MLH1 to chromatin (By similarity). Interacts with MSH2; the interaction recruits MCM9 to chromatin (By similarity). Interacts with MSH6 (By similarity). Interacts with the MRN complex composed of MRE11, RAD50 and NBN/NBS1; the interaction recruits the MRN complex to DNA damage sites (By similarity). Interacts with RAD51; the interaction recruits RAD51 to DNA damage sites (By similarity).
Component of the MCM8-MCM9 complex, which forms a hexamer composed of MCM8 and MCM9 (PubMed:23401855, PubMed:26300262, PubMed:26215093). Interacts with the DNA mismatch repair (MMR) complex composed at least of MSH2, MSH3, MSH6, PMS1 and MLH1 (PubMed:26300262). Interacts with MLH1; the interaction recruits MLH1 to chromatin (PubMed:26300262). Interacts with MSH2; the interaction recruits MCM9 to chromatin (PubMed:26300262). Interacts with MSH6 (PubMed:26300262). Interacts with the MRN complex composed of MRE11, RAD50 and NBN/NBS1; the interaction recruits the MRN complex to DNA damage sites (PubMed:26215093). Interacts with RAD51; the interaction recruits RAD51 to DNA damage sites (PubMed:23401855).
Component of the MCM8-MCM9 complex, which forms a hexamer composed of mcm8 and mcm9. Interacts with cdt1 (By similarity).

Similarity

Belongs to the MCM family.

Keywords

Alternative splicing   ATP-binding   Chromosome   Complete proteome   DNA damage   DNA repair   DNA-binding   Helicase   Hydrolase   Nucleotide-binding   Nucleus   Phosphoprotein   Reference proteome   DNA replication  

Feature

chain  DNA helicase MCM9
splice variant  In isoform 2.

Uniprot

A0A2A4JLZ1   A0A2W1BW94   A0A194QBS7   A0A212EIY5   A0A194QXS1   A0A067RI06   + More
A0A1B6C633   K1PZI7   A0A210PG05   A0A1S3GJ75   V8PAR1   A0A2Y9HI90   A0A2K6T8N1   A0A2R8ZWC4   A0A093I0Q0   A0A384AUU8   A0A2J8TAG6   Q2KHI9   Q9NXL9   Q2KHI9-2   G3U4T6   A0A1W4ZB32   G3TC88   F6RIX4   A0A3B3SWV2   A0A340X4S7   W4XJQ6   A0A2G8KKN5   A0A3N0Z2H5   D6WB76   A0A146MDY4   W5LF76   A0A0A9XC16   M3XF20   A0A2C9JZD6  

EC Number

3.6.4.12

Pubmed

28756777    26354079    22118469    24845553    22992520    28812685    + More
24297900    22722832    19468303    15489334    16141072    16226853    15850810    21987787    22771120    23401855    26300262    14702039    14574404    18669648    20068231    23403237    23186163    25480036    26215093    20431018    29240929    29023486    18362917    19820115    26823975    25329095    25401762    17975172    15562597   

EMBL

NWSH01001141    PCG72422.1    KZ149899    PZC78611.1    KQ459249    KPJ02445.1    + More
AGBW02014560    OWR41431.1    KQ461155    KPJ08376.1    KK852460    KDR23496.1    GEDC01028335    JAS08963.1    JH819194    EKC29607.1    NEDP02076730    OWF35387.1    AZIM01000436    ETE71083.1    AJFE02097141    AJFE02097142    AJFE02097143    AJFE02097144    KL206669    KFV84797.1    NDHI03003512    PNJ30010.1    PNJ30014.1    AC153949    AC155941    BC062185    AK018494    AK046636    BN000883    AK299076    AK000177    AL132874    AL359634    BC031658    BN000882    AAMC01008549    AAGJ04116641    MRZV01000515    PIK48566.1    RJVU01015592    ROL52501.1    KQ971312    EEZ97925.1    GDHC01000621    JAQ18008.1    GBHO01026428    JAG17176.1    AANG04000169   

Proteomes

UP000218220    UP000053268    UP000007151    UP000053240    UP000027135    UP000005408    + More
UP000242188    UP000081671    UP000248481    UP000233220    UP000240080    UP000053584    UP000000589    UP000005640    UP000007646    UP000192224    UP000008143    UP000261540    UP000265300    UP000007110    UP000230750    UP000007266    UP000018467    UP000011712    UP000076420   

PRIDE

Q2KHI9    Q9NXL9    F6RIX4   

Pfam

PF17855   MCM_lid        + More
PF00493   MCM
PF17207   MCM_OB
PF08799   PRP4
PF02840   Prp18

Interpro

IPR001208   MCM_dom        + More
IPR031327   MCM       
IPR003593   AAA+_ATPase       
IPR012340   NA-bd_OB-fold       
IPR027417   P-loop_NTPase       
IPR033762   MCM_OB       
IPR041562   MCM_lid       
IPR004098   Prp18       
IPR036285   PRP4-like_sf       
IPR014906   PRP4-like       
IPR018525   MCM_CS       

SUPFAM

SSF50249   SSF50249        + More
SSF52540   SSF52540       
SSF158230   SSF158230       

Gene 3D

1.10.720.150    

ProteinModelPortal

A0A2A4JLZ1   A0A2W1BW94   A0A194QBS7   A0A212EIY5   A0A194QXS1   A0A067RI06   + More
A0A1B6C633   K1PZI7   A0A210PG05   A0A1S3GJ75   V8PAR1   A0A2Y9HI90   A0A2K6T8N1   A0A2R8ZWC4   A0A093I0Q0   A0A384AUU8   A0A2J8TAG6   Q2KHI9   Q9NXL9   Q2KHI9-2   G3U4T6   A0A1W4ZB32   G3TC88   F6RIX4   A0A3B3SWV2   A0A340X4S7   W4XJQ6   A0A2G8KKN5   A0A3N0Z2H5   D6WB76   A0A146MDY4   W5LF76   A0A0A9XC16   M3XF20   A0A2C9JZD6  

PDB

3F9V     E-value=2.13819e-77,     Score=739

KEGG

105842269    K10738 DNA helicase MCM9 [EC:3.6.4.12] | (RefSeq) DNA helicase MCM9-like

GO

GO:0003677   GO:0006270   GO:0005524   GO:0008380   GO:0005681   GO:0004386   GO:0097362   GO:0032406   GO:0032408   GO:0000724   GO:0032407   GO:0070716   GO:0005634   GO:0007292   GO:0003678   GO:0019899   GO:0036298   GO:0071168   GO:0003682   GO:0032508   GO:0006974   GO:0007276   GO:0044877   GO:0003688   GO:0042555   GO:0003697   GO:0005694   GO:0006260   GO:0003676   GO:0004352   GO:0000287   GO:0003723   GO:0048384  

PANTHER

PTHR11630  

Subcellular location

Nucleus   Colocalizes to nuclear foci with RPA1 following DNA damage (PubMed:23401855). Localizes to double-stranded DNA breaks (By similarity). Recruited to chromatin by MSH2 (By similarity).   With evidence from 1 publications.
Chromosome   Colocalizes to nuclear foci with RPA1 following DNA damage (PubMed:23401855). Localizes to double-stranded DNA breaks (By similarity). Recruited to chromatin by MSH2 (By similarity).   With evidence from 1 publications.

Length:

944

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.0087

Exp number, first 60 AAs:

0

Total prob of N-in:

0.00028

outside

1  -  944

 

 

Pi

203.925675

Theta

195.770812

Tajima's D

-1.796531

CLR

128.875481

CSRT

0.0284985750712464

Interpretation

Uncertain