Gene

KWMTBOMO03473  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA006523

Annotation

PREDICTED:_LOW_QUALITY_PROTEIN:_glucosidase_2_subunit_beta-like_[Bombyx_mori]

Location

Bomo_Chr6(+):8809313-8811059      View in EpiBrowser

Full name

Glucosidase 2 subunit beta      

Alternative Name

80K-H protein
Glucosidase II subunit beta
Protein kinase C substrate 60.1 kDa protein heavy chain

Location in the cell

Cytoplasmic   Reliability : 1.617 Nuclear   Reliability : 2.209

CDS

ATGGTCTGTAAAACATGGATCGACCGATTTTCCTCCTATTTCGTAATATTTTGTTCCGTCATCATATTTGCCCAGTCCGATGTGCCTAGACCCCGCGGTGTATCTTTGTCTAAAGCATCTCTCTATTTACCGACAAAAGATTTTACTTGTTTTGATGGAACTGCTACAATACCTTTCAGCTACGTTAATGACGACTACTGTGATTGTTTCGACGGTAGCGATGAACCCGGTACATCGGCTTGTATAAACGGTGTTTTCCATTGTACAAATGCCGGACACCGGCCTCAGAATTTGCCGAGTTCCAGGGTTAATGACGGAGTCTGTGATTGCTGTGATGGAACAGATGAATACGCTAATCCTACGGCCTGTACTAACATTTGTGAAGAGCTCGGAAAAGAAGCCCGAGCAGAAGCTCAAAGAGTAGCAGAGTTGCATAAAGCAGGCAGCCAATTGAGAATAGACCTCATAGAAAAAGGAAATAAGAAGCGAAATGAAATGGCAGAACAACTAACACAACTTGAAAAAGACAAATCTGAAGCAGAAAAAATAAAGGCTGAAAAAGAGCTTCTAAAGAATGATCTAGAAATGAAAGAAAATGAGGTTTTGAAAGTATATAGAGAAGCAGAAGAATTGGAAAAACAAAAGAAATTAGACCAAGAGAAAGAAACTAATTTAAAAGAATCAACGGAACACTTTAATAGATTTGATTCAAATAATGATGGAGAGTTAAGTATAGATGAAATCAAAGTTGTTAATGTATTTGATAGGAACAAGGATGGCCAAGTTGATGATGATGAAGTAAAGATGTTCTTGGGTGGTGATGAAAAAGTAGATAAAGATGCATTCTTGTCCACAACTTGGCCTATCTTAAAACCACTTTTGATGATGGAGCAAGGTATGTTTAAACCAGAGGATGCTAAAACAGATACCACGGAGACAGAAGCTGAAGAAAACCATGAAGAATTGGATGCTGATTTTGAAGGAAATGAAGAAGATAATAGCGATTTAGATTTACAAGAAGAACATGAAGAAGATGAGACTGTGTCAGAGCAGTCAAAATCATATAATGATGAGACTCAAAAAATTGTTGATCAGGCTTCAGAAGCTCGCAGGCAATACACAGATGCTGAGCGAACTGTTAGAGAAATAGAGTCTAACATTAGAAACATAAAACAGAACTTAGAAAAGGATTATGGTCTAGAACAGGAATTTGCTTCCTTGGATGGTGATTGCTTTGAATATGAAGACAAAGAATATGTATACAAACTGTGTATGTTCCAGAAGGTAACACAAAAATCCAAGAATGGTGGTATGGAGGTAGGTCTTGGTAATTGGGGAGAATGGGCAGGTCCTGAAAACAATAAATACTCTGTGATGAAATACACAAATGGAATTGCTTGCTGGAATGGTCCAAGTCGAACAACAACTGTTAATGTCAACTGTGACTTGGAAACAAAGATCACATCAGTAACTGAACCTTTCCGTTGTGAATACAAAATGGAATTAAGCACTCCTGCTGCATGTGATGATTCTAGCACATCACAAGAGCATACATCACATGATGAACTTTAA

Protein

MVCKTWIDRFSSYFVIFCSVIIFAQSDVPRPRGVSLSKASLYLPTKDFTCFDGTATIPFSYVNDDYCDCFDGSDEPGTSACINGVFHCTNAGHRPQNLPSSRVNDGVCDCCDGTDEYANPTACTNICEELGKEARAEAQRVAELHKAGSQLRIDLIEKGNKKRNEMAEQLTQLEKDKSEAEKIKAEKELLKNDLEMKENEVLKVYREAEELEKQKKLDQEKETNLKESTEHFNRFDSNNDGELSIDEIKVVNVFDRNKDGQVDDDEVKMFLGGDEKVDKDAFLSTTWPILKPLLMMEQGMFKPEDAKTDTTETEAEENHEELDADFEGNEEDNSDLDLQEEHEEDETVSEQSKSYNDETQKIVDQASEARRQYTDAERTVREIESNIRNIKQNLEKDYGLEQEFASLDGDCFEYEDKEYVYKLCMFQKVTQKSKNGGMEVGLGNWGEWAGPENNKYSVMKYTNGIACWNGPSRTTTVNVNCDLETKITSVTEPFRCEYKMELSTPAACDDSSTSQEHTSHDEL

Description

Regulatory subunit of glucosidase II that cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins (PubMed:27462106, PubMed:9148925). Required for efficient PKD1/Polycystin-1 biogenesis and trafficking to the plasma membrane of the primary cilia (PubMed:21685914).

Subunit

Heterodimer of a catalytic alpha subunit (GANAB) and a beta subunit (PRKCSH) (PubMed:9148925, PubMed:27462106). Binds glycosylated PTPRC (PubMed:9148925).

Keywords

3D-structure   Alternative splicing   Calcium   Complete proteome   Direct protein sequencing   Disulfide bond   Endoplasmic reticulum   Glycoprotein   Metal-binding   Phosphoprotein   Reference proteome   Repeat   Signal  

Feature

chain  Glucosidase 2 subunit beta
splice variant  In isoform 2.

Uniprot

L8B6C5   A0A1E1WUQ0   A0A345W862   A0A194PVQ6   A0A194RQ52   S4PCP7   + More
A0A2A4J8T2   A0A2W1BRF2   A0A212F655   A0A2H1VSG9   A0A0L7LAG7   I4DNV0   D6W9R4   A0A1Y1M7I2   V5G2I9   A0A1B6D3J4   A0A1B0CR59   A0A1L8DJ45   A0A1L8DJ39   A0A1L8DM68   A0A1L8DM51   A0A0N1IT49   A0A1Q3FF99   A0A2P8XVP6   A0A0P4ZG66   A0A0P4YW50   A0A1D2N278   A0A088AUN3   A0A0P4WUM2   A0A0L7R7R0   A0A0P5Z9C2   A0A0P5RRM1   A0A0P5Y5P3   A0A0P5IT85   A0A0J7KXH9   E9G8J3   A0A2A3ER50   A0A182IWR0   U4TZH6   N6T690   E9IKT6   A0A154PSW3   A0A195FCW2   E2AQZ0   A0A195EL25   A0A158NZI4   A0A0P6I4Q2   A0A023EUW9   B0WS45   A0A151WR87   J9JPV0   A0A2H8TH21   A0A0P5DIB6   A0A0P6BRN7   A0A195CJ66   K7J4G8   A0A3L8D713   F4WL80   A0A026W1M5   B3MMH5   B4N1C4   B4I5B3   A0A1A9WM41   A0A0J9TQ58   Q9VJD1   A0A1B6GD55   B4P8R1   A0A132A4G4   Q29LB3   B4GQB0   A0A1A9Y5B2   A0A1S3JT06   B3NMG7   A0A1J1HJU0   A0A1B0A3X9   A0A0A1XJ19   A0A3B0JCE6   W8BAL3   R4WR17   A0A310TPI7   A0A1W4ULN9   Q6AZH1   A0A2S2Q9G1   A0A034WM66   A0A0N8JZG3   Q6GLZ2   A0A1B0FH60   A0A131XVY0   A0A3N0YY10   A8WGX5   Q6P373   O08795-2   A0A0K8VHR2   A0A0P6J571   G3UJW5   H0XEE9   A0A2K5WQ22   A0A0P5CQM8   F7C6C6  

Pubmed

23376632    29258441    26354079    23622113    28756777    22118469    + More
26227816    22651552    18362917    19820115    28004739    29403074    27289101    21292972    23537049    21282665    20798317    21347285    24945155    20075255    30249741    21719571    24508170    17994087    22936249    10731132    12537568    12537572    12537573    12537574    16110336    17569856    17569867    26109357    26109356    17550304    26555130    15632085    25830018    24495485    23691247    25348373    9148925    15489334    21183079    21685914    23806337    27462106    17431167   

EMBL

AB747259    BAM78679.1    GDQN01000387    JAT90667.1    MH071439    AXJ21600.1    + More
KQ459589    KPI97471.1    KQ459896    KPJ19450.1    GAIX01004066    JAA88494.1    NWSH01002631    PCG67803.1    KZ150004    PZC75256.1    AGBW02010074    OWR49217.1    ODYU01004177    SOQ43767.1    JTDY01002064    KOB72196.1    AK403151    BAM19590.1    KQ971312    EEZ98119.2    GEZM01038259    JAV81683.1    GALX01004236    JAB64230.1    GEDC01017026    JAS20272.1    AJWK01024420    GFDF01007596    JAV06488.1    GFDF01007595    JAV06489.1    GFDF01006630    JAV07454.1    GFDF01006545    JAV07539.1    KQ435883    KOX69658.1    GFDL01008834    JAV26211.1    PYGN01001278    PSN36075.1    GDIP01221092    GDIP01213238    GDIP01137490    GDIP01130596    GDIP01130595    GDIP01127834    GDIP01113000    GDIP01061107    LRGB01002451    JAJ10164.1    KZS07500.1    GDIP01222238    GDIP01222237    GDIP01184635    GDIP01156603    GDIP01146489    GDIP01146488    GDIQ01136981    GDIP01111739    JAJ01165.1    JAL14745.1    LJIJ01000284    ODM99396.1    GDIP01251373    GDIP01221093    GDIP01140845    GDIP01140844    GDIP01137492    GDIP01137491    GDIP01130594    GDIP01113002    GDIP01113001    GDIP01074409    JAI72028.1    KQ414638    KOC66858.1    GDIP01222239    GDIP01184634    GDIP01115742    GDIP01111738    GDIP01085060    GDIP01073158    GDIP01065109    GDIP01047016    GDIP01047015    JAM56699.1    GDIQ01102850    JAL48876.1    GDIP01062603    JAM41112.1    GDIQ01208311    JAK43414.1    LBMM01002287    KMQ95026.1    GL732535    EFX84260.1    KZ288192    PBC34245.1    KB631903    ERL87019.1    APGK01042136    KB741002    ENN75729.1    GL764026    EFZ18732.1    KQ435075    KZC14414.1    KQ981685    KYN38037.1    GL441846    EFN64136.1    KQ978747    KYN28642.1    ADTU01004641    GDIQ01009391    JAN85346.1    GAPW01000816    JAC12782.1    DS232064    EDS33664.1    KQ982807    KYQ50376.1    ABLF02023169    GFXV01001525    MBW13330.1    GDIP01161692    JAJ61710.1    GDIP01025764    JAM77951.1    KQ977720    KYN00477.1    QOIP01000012    RLU16255.1    GL888207    EGI65043.1    KK107488    EZA49928.1    CH902620    EDV30921.1    CH963920    EDW78114.1    CH480822    EDW55569.1    CM002910    KMY90720.1    AE014134    AY058725    AAF53621.1    AAL13954.1    AFH03737.1    GECZ01009391    JAS60378.1    CM000158    EDW90169.1    JXLN01010490    KPM05814.1    CH379060    EAL34132.2    CH479187    EDW39782.1    CH954179    EDV54838.1    CVRI01000006    CRK88328.1    GBXI01003286    JAD11006.1    OUUW01000004    SPP80034.1    GAMC01010828    JAB95727.1    AK418136    BAN21351.1    KV467250    OCT56758.1    BC078024    AAH78024.1    GGMS01005170    MBY74373.1    GAKP01004074    JAC54878.1    JARO02004047    KPP69257.1    BC074301    AAH74301.1    CCAG010009633    GEFM01004607    JAP71189.1    RJVU01019149    ROL51195.1    BC154890    AAI54891.1    BC064160    AAH64160.1    U92794    BC009816    GDHF01014922    GDHF01013888    JAI37392.1    JAI38426.1    GEBF01005937    JAN97695.1    AAQR03140653    AAQR03140654    AAQR03140655    AAQR03140656    AQIA01034305    AQIA01034306    AQIA01034307    GDIP01170884    JAJ52518.1    JSUE03020790    JSUE03020791   

Proteomes

UP000053268    UP000053240    UP000218220    UP000007151    UP000037510    UP000007266    + More
UP000092461    UP000053105    UP000245037    UP000076858    UP000094527    UP000005203    UP000053825    UP000036403    UP000000305    UP000242457    UP000075880    UP000030742    UP000019118    UP000076502    UP000078541    UP000000311    UP000078492    UP000005205    UP000002320    UP000075809    UP000007819    UP000078542    UP000002358    UP000279307    UP000007755    UP000053097    UP000007801    UP000007798    UP000001292    UP000091820    UP000000803    UP000002282    UP000001819    UP000008744    UP000092443    UP000085678    UP000008711    UP000183832    UP000092445    UP000268350    UP000192221    UP000034805    UP000092444    UP000000589    UP000007646    UP000005225    UP000233100    UP000006718   

PRIDE

L8B6C5    A0A088AUN3    Q9VJD1    O08795   

Pfam

PF13015   PRKCSH_1        + More
PF12999   PRKCSH-like
PF13202   EF-hand_5
PF07915   PRKCSH

Interpro

IPR009011   Man6P_isomerase_rcpt-bd_dom_sf        + More
IPR011992   EF-hand-dom_pair       
IPR002048   EF_hand_dom       
IPR036055   LDL_receptor-like_sf       
IPR036607   PRKCSH       
IPR026874   Glucosidase_2_bsu       
IPR039794   Gtb1-like       
IPR018247   EF_Hand_1_Ca_BS       
IPR028146   PRKCSH_N       
IPR002172   LDrepeatLR_classA_rpt       
IPR012913   OS9-like       

SUPFAM

SSF47473   SSF47473        + More
SSF57424   SSF57424       

Gene 3D

2.70.130.10     4.10.400.10    

CDD

cd00112  LDLa
cd00051  EFh

ProteinModelPortal

L8B6C5   A0A1E1WUQ0   A0A345W862   A0A194PVQ6   A0A194RQ52   S4PCP7   + More
A0A2A4J8T2   A0A2W1BRF2   A0A212F655   A0A2H1VSG9   A0A0L7LAG7   I4DNV0   D6W9R4   A0A1Y1M7I2   V5G2I9   A0A1B6D3J4   A0A1B0CR59   A0A1L8DJ45   A0A1L8DJ39   A0A1L8DM68   A0A1L8DM51   A0A0N1IT49   A0A1Q3FF99   A0A2P8XVP6   A0A0P4ZG66   A0A0P4YW50   A0A1D2N278   A0A088AUN3   A0A0P4WUM2   A0A0L7R7R0   A0A0P5Z9C2   A0A0P5RRM1   A0A0P5Y5P3   A0A0P5IT85   A0A0J7KXH9   E9G8J3   A0A2A3ER50   A0A182IWR0   U4TZH6   N6T690   E9IKT6   A0A154PSW3   A0A195FCW2   E2AQZ0   A0A195EL25   A0A158NZI4   A0A0P6I4Q2   A0A023EUW9   B0WS45   A0A151WR87   J9JPV0   A0A2H8TH21   A0A0P5DIB6   A0A0P6BRN7   A0A195CJ66   K7J4G8   A0A3L8D713   F4WL80   A0A026W1M5   B3MMH5   B4N1C4   B4I5B3   A0A1A9WM41   A0A0J9TQ58   Q9VJD1   A0A1B6GD55   B4P8R1   A0A132A4G4   Q29LB3   B4GQB0   A0A1A9Y5B2   A0A1S3JT06   B3NMG7   A0A1J1HJU0   A0A1B0A3X9   A0A0A1XJ19   A0A3B0JCE6   W8BAL3   R4WR17   A0A310TPI7   A0A1W4ULN9   Q6AZH1   A0A2S2Q9G1   A0A034WM66   A0A0N8JZG3   Q6GLZ2   A0A1B0FH60   A0A131XVY0   A0A3N0YY10   A8WGX5   Q6P373   O08795-2   A0A0K8VHR2   A0A0P6J571   G3UJW5   H0XEE9   A0A2K5WQ22   A0A0P5CQM8   F7C6C6  

PDB

5IEG     E-value=4.31322e-30,     Score=329

KEGG

101746261    K08288 protein kinase C substrate 80K-H | (RefSeq) glucosidase 2 subunit beta-like

PATHWAY

04141    Protein processing in endoplasmic reticulum - Bombyx mori (domestic silkworm)

GO

GO:0005509   GO:0006491   GO:0016021   GO:0012505   GO:0016301   GO:0010977   GO:0051291   GO:0043231   GO:0003723   GO:0005783   GO:0044325   GO:0051219   GO:0017177   GO:0001701   GO:0001889   GO:0005080   GO:0006807   GO:0005515   GO:0008270   GO:0016491   GO:0004352   GO:0000287   GO:0006270   GO:0042555   GO:0048384  

PANTHER

PTHR12630   PTHR12630:SF1  

Subcellular location

Endoplasmic reticulum  

SignalP

Position:   1 - 26,         Likelihood:  0.766809

 

 

Length:

523

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

10.10789

Exp number, first 60 AAs:

10.10786

Total prob of N-in:

0.53794

POSSIBLE N-term signal

sequence

outside

1  -  523

 

 

Pi

54.585553

Theta

128.088827

Tajima's D

-1.301558

CLR

370.208842

CSRT

0.0893955302234888

Interpretation

Uncertain