Gene

KWMTBOMO03430  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA006507

Annotation

glutamate_dehydrogenase_[Bombyx_mori]

Location

Bomo_Chr6(+):7710564-7733783      View in EpiBrowser

Full name

Glutamate dehydrogenase       + More
Glutamate dehydrogenase, mitochondrial      

Location in the cell

Cytoplasmic   Reliability : 2.33

CDS

ATGCTGCATCTCAAGAATATCGCCAAGTCAGTGGTTCCGCCGCTCAAGAATTCTGTTCAAAATGAAGCCCTCAATACAATGTTCCGAATCATACCAGCTGGAGTGAATGTCTGCTGCCGCACATACGCTAGTCATGAGATTCCAGATAAGCTCAAAGATATTCCTACAAGTGCGAATCCGAAGTTCTTCCACATGGTAGAATATTTTTTCCACCGAGCCTGTCAAGTTGTCGAAGACAAGCTTGTTGAAGATTTGAAGTCAAGGACACCCATTGAAGAGAAGAAAAAGAAAGTAGCCGGTATTCTAAAACTTATGGAACCATGCGATCACATTCTTGAGATTCAATTTCCTCTGAGGCGCGATTCTGGCGATTACGAAATGATATTAGGCTATCGCGCACAACATTCCACACACAGGACTCCAACCAAAGGAGGTATTCGATTCTCAACGGACGTAACCAGAGATGAAGTTAAGGCGTTATCAGCTTTGATGACCTTCAAGTGCGCGTGCGTGGACGTGCCTTTCGGCGGTGCTAAGGCCGGTATCAAGATCAATCCCAAAGAATACTCCGAGCATGAACTGGAAAAGATCACTCGTCGTTTCACCCTTGAACTTGCCAAAAAAGGATTCATTGGGCCTGGCGTGGATGTCCCCGCTCCTGACATGGGTACCGGCGAACGAGAAATGTCTTGGATCGCCGATACTTATGCGAAGACCGTCGGTTTTCAAGACATCAACGCTCACGCCTGCGTCACTGGCAAACCTATTAACCAGGGTGGCATCCACGGCAGAGTTTCAGCCACGGGCAGGGGCGTATTCCACGGCTTGGAGAACTTCATCAACGAAGCCAACTACATGAGCATGATCGGTACAACCCCCGGTTGGGGTGGCAAGACGTTCATCGTCCAAGGTTTCGGTAACGTGGGACTCCACACTTGCCGCTACCTCGTCCGCGCCGGCGCCACTTGCATCGGAGTTATCGAGCACGACGGCTCCATTTACAACCCTGATGGCATCAACCCTAAGGCCTTGGAGGACTACAGAATCGAGAACGGTACGGTAGTCGGTTTCCCCGGCGCTAAGGCCTACGAAGGCGAGAACATGCTTTACGAGAAGTGCGACATTCTTGTACCCGCCGCCATCGAACAGGTCATAAACAAGGACAACGCTCACAGGATCCAAGCTAAGATCATTGCGGAGGCCGCCAACGGTCCCACCACACCTGCTGCAGACAAGATCCTCATCGATCGCAACATTCTCGTGATCCCCGACCTCTACATCAACGCTGGTGGTGTCACCGTCTCATTCTTCGAGTGGCTCAAGAACCTCAATCACGTGTCTTACGGACGTCTGACATTCAAATACGAGAGGGAATCTAACTACCATCTGCTGGAATCGGTCCAAGAGTCTCTCGAGCGGCGGTTCGGTCGCGTGGGAGGCCGCATCCCCGTCACTCCCTCAGAGTCCTTCCAGAAGAGAATCTCCGGCGCCTCCGAGAAGGACATCGTGCACTCCGGACTCGACTACACCATGGAGAGATCCGCTAGGGCCATCATGAAGACAGCCATGAGGTTCAACCTCGGTTTAGATCTGAGGACAGCCGCGTATGCGAACTCCATCGAAAAGATATTCACCACTGTCGAGTTAACGTTTGAACTTCCTGATAGTGCCGAAGAATGTTTTTATCAAACAATGGACAAGGATACGGCTGCTTCATTGGAGTACCAAGTAATTTCTGGAGGACAGTATGACGTAAGCGTAAAAATAGAAGCTCCTAACAGACAAATTATATATCAGCAAGAGAAGATGCAATATGATTCACACCAGTTCACCGCTCTGGCGTCTGGTGATTACAAAGTGTGTTTTAGTAATGAGTTTAGTACATTTTCACATAAACTTGTGTATATGGAACTAAATGTTGGACCGGAGCAGCCTCTTCCTGGTGTAGGAGATCATGCAACTGTTCTTACACAGTTGGAGACATCTGCCGAAGAAATTCATTCTGCACTCAACAGAATCATTGATCATCAAACTCATCATAGATTAAGAGAGGCTCAAGGACGTAAAAGAGCTGAAGATCTGAATGAAAAAGTATTTTGGTGGTCTATGGGTGAAACCTTGGCTATTGTTTGTGTTGCATTTACACAAGTTATGATACTAAAGAATTTCTTTAGTGACAAGCCTACTCTATACAACCGATTGTAA

Protein

MLHLKNIAKSVVPPLKNSVQNEALNTMFRIIPAGVNVCCRTYASHEIPDKLKDIPTSANPKFFHMVEYFFHRACQVVEDKLVEDLKSRTPIEEKKKKVAGILKLMEPCDHILEIQFPLRRDSGDYEMILGYRAQHSTHRTPTKGGIRFSTDVTRDEVKALSALMTFKCACVDVPFGGAKAGIKINPKEYSEHELEKITRRFTLELAKKGFIGPGVDVPAPDMGTGEREMSWIADTYAKTVGFQDINAHACVTGKPINQGGIHGRVSATGRGVFHGLENFINEANYMSMIGTTPGWGGKTFIVQGFGNVGLHTCRYLVRAGATCIGVIEHDGSIYNPDGINPKALEDYRIENGTVVGFPGAKAYEGENMLYEKCDILVPAAIEQVINKDNAHRIQAKIIAEAANGPTTPAADKILIDRNILVIPDLYINAGGVTVSFFEWLKNLNHVSYGRLTFKYERESNYHLLESVQESLERRFGRVGGRIPVTPSESFQKRISGASEKDIVHSGLDYTMERSARAIMKTAMRFNLGLDLRTAAYANSIEKIFTTVELTFELPDSAEECFYQTMDKDTAASLEYQVISGGQYDVSVKIEAPNRQIIYQQEKMQYDSHQFTALASGDYKVCFSNEFSTFSHKLVYMELNVGPEQPLPGVGDHATVLTQLETSAEEIHSALNRIIDHQTHHRLREAQGRKRAEDLNEKVFWWSMGETLAIVCVAFTQVMILKNFFSDKPTLYNRL

Catalytic Activity

H2O + L-glutamate + NAD(+) = 2-oxoglutarate + H(+) + NADH + NH4(+)
H2O + L-glutamate + NADP(+) = 2-oxoglutarate + H(+) + NADPH + NH4(+)

Subunit

Homohexamer.

Miscellaneous

ADP can occupy the NADH binding site and activate the enzyme.

Similarity

Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Keywords

Alternative splicing   ATP-binding   Complete proteome   GTP-binding   Mitochondrion   NAD   Nucleotide-binding   Oxidoreductase   Reference proteome   Transit peptide  

Feature

chain  Glutamate dehydrogenase, mitochondrial
splice variant  In isoform C.

Uniprot

Q2F5R4   A0A2A4JCS9   A0A0G3VFZ9   A0A194Q273   S4PE01   I4DQZ0   + More
A0A194RNK3   I4DJ42   A0A0K8TT23   E2C3C7   E9IPG3   A0A310SFZ1   A0A151X7U3   A0A2A3EPX9   A0A087ZS68   E2A6U2   A0A2J7PVJ1   A0A195FTW7   A0A154PIZ3   A0A158NNF9   W8BYK3   A0A1J1IUJ7   A0A068TJS2   A0A026WII5   A0A034VPU7   A0A0A1XFV2   A0A1I8NSV1   A0A1B6GVV3   A0A336MGF4   T1PBD9   A0A232F5M0   K7IYA3   A0A0M9AAI4   A0A195C1R0   A0A1L8EEM7   A0A158NNF3   A0A1B6EYJ5   A0A1L8E2M4   P54385-2   A0A0P8ZYJ0   A0A0Q9WUT7   A0A0R1EBF8   A0A182QQS6   A0A1W4U8M3   A0A1J1IWE9   A0A034VKL6   A0A2M4CUS3   A0A0Q9WR91   A0A3B0KCM3   A0A1I8NSU8   I5ANX6   A0A2M4CT81   A0A0C9Q076   A0A0Q9X1Y5   A0A0A1X040   A0A1A9Y0I5   A0A1B0B2Z8   T1P987   A0A1A9W5Q9   D3TQR2   A0A1A9ZSU9   A0A0K8UFK5   A0A2M3ZZI4   A0A336KTS7   A0A182R8H6   A0A1L8EER7   F5HLL7   A0A2M3YYJ1   B3M317   A0A0L0BRA3   A0A182XF30   A0A0M3QYL6   B4N9E8   B4PL48   B3P8B7   P54385   A0A1W4UJV6   B4QSD4   U5EZ79   A0A3B0JQU1   B4HG10   Q298M7   B4G4C1   A0A182LW14   B4K740   B4M0C5   A0A182PG80   A0A023ETW6   A0A0L7RDB5   B4JRQ0   Q2KQ98   A0A2M3ZZC8   A0A182IW02   A0A182V234   A0A182UC31   A0A182HVJ8   A0A182YJQ2  

EC Number

1.4.1.3

Pubmed

19121390    26354079    23622113    22651552    26369729    20798317    + More
21282665    21347285    24495485    24508170    25348373    25830018    25315136    28648823    20075255    10992165    10731132    12537572    12537569    6810872    17994087    17550304    15632085    20353571    12364791    14747013    17210077    26108605    24945155    26483478    15804581    17510324    25244985   

EMBL

BABH01009287    BABH01009288    BABH01009289    BABH01009290    DQ311359    ABD36303.1    + More
NWSH01001856    PCG69907.1    KP657639    RSAL01000283    AKL78864.1    RVE43001.1    KQ459589    KPI97505.1    GAIX01003416    JAA89144.1    AK404850    BAM20330.1    KQ459896    KPJ19418.1    AK401310    BAM17932.1    GDAI01000089    JAI17514.1    GL452320    EFN77465.1    GL764503    EFZ17554.1    KQ760382    OAD60710.1    KQ982431    KYQ56433.1    KZ288204    PBC33189.1    GL437203    EFN70808.1    NEVH01020956    PNF20354.1    KQ981268    KYN43903.1    KQ434931    KZC11856.1    ADTU01002703    ADTU01002704    ADTU01002705    ADTU01002706    ADTU01002707    ADTU01002708    ADTU01002709    ADTU01002710    ADTU01002711    ADTU01002712    GAMC01008174    JAB98381.1    CVRI01000060    CRL03893.1    HG965788    CDO39384.1    KK107234    EZA54914.1    GAKP01015117    JAC43835.1    GBXI01004909    JAD09383.1    GECZ01003209    GECZ01000641    JAS66560.1    JAS69128.1    UFQS01000897    UFQT01000897    SSX07543.1    SSX27883.1    KA645293    AFP59922.1    NNAY01000875    OXU26104.1    KQ435714    KOX79199.1    KQ978344    KYM94782.1    GFDG01001617    JAV17182.1    GECZ01026752    GECZ01010743    JAS43017.1    JAS59026.1    GFDF01001150    JAV12934.1    Y11314    Z29062    AE014297    AY061323    BT001501    CH902617    KPU79715.1    CH964232    KRF99304.1    CM000160    KRK04628.1    AXCN02000334    CRL03892.1    GAKP01015116    JAC43836.1    GGFL01004733    MBW68911.1    CH940650    KRF83230.1    OUUW01000007    SPP82761.1    CM000070    EIM52661.1    GGFL01004352    MBW68530.1    GBYB01007233    JAG77000.1    CH933806    KRG02059.1    GBXI01009825    JAD04467.1    JXJN01007741    JXJN01007742    JXJN01007743    KA645292    AFP59921.1    EZ423764    ADD20040.1    GDHF01026885    JAI25429.1    GGFK01000665    MBW33986.1    SSX07542.1    SSX27882.1    GFDG01001619    JAV17180.1    AAAB01008898    EGK97150.1    GGFM01000592    MBW21343.1    EDV42417.1    JRES01001482    KNC22546.1    CP012526    ALC47893.1    EDW80581.1    EDW99033.1    CH954182    EDV53941.1    CM000364    EDX14133.1    GANO01001279    JAB58592.1    SPP82762.1    CH480815    EDW43403.1    EAL27928.2    CH479179    EDW24469.1    AXCM01000759    EDW16353.1    EDW67287.1    JXUM01016134    JXUM01016135    GAPW01000805    KQ560450    JAC12793.1    KXJ82361.1    KQ414615    KOC68746.1    CH916373    EDV94440.1    AY623405    CH477664    AAV31914.1    EAT37557.1    EAT37559.1    GGFK01000605    MBW33926.1    APCN01005788   

Proteomes

UP000005204    UP000218220    UP000283053    UP000053268    UP000053240    UP000008237    + More
UP000075809    UP000242457    UP000005203    UP000000311    UP000235965    UP000078541    UP000076502    UP000005205    UP000183832    UP000053097    UP000095300    UP000095301    UP000215335    UP000002358    UP000053105    UP000078542    UP000000803    UP000007801    UP000007798    UP000002282    UP000075886    UP000192221    UP000008792    UP000268350    UP000001819    UP000009192    UP000092443    UP000092460    UP000091820    UP000092445    UP000075900    UP000007062    UP000037069    UP000076407    UP000092553    UP000008711    UP000000304    UP000001292    UP000008744    UP000075883    UP000075885    UP000069940    UP000249989    UP000053825    UP000001070    UP000008820    UP000075880    UP000075903    UP000075902    UP000075840    UP000076408   

PRIDE

Q2F5R4    A0A087ZS68    P54385   

Pfam

PF02812   ELFV_dehydrog_N        + More
PF00208   ELFV_dehydrog
PF14969   DUF4508

Interpro

IPR006095   Glu/Leu/Phe/Val_DH        + More
IPR006096   Glu/Leu/Phe/Val_DH_C       
IPR006097   Glu/Leu/Phe/Val_DH_dimer_dom       
IPR033922   NAD_bind_Glu_DH       
IPR036291   NAD(P)-bd_dom_sf       
IPR033524   Glu/Leu/Phe/Val_DH_AS       
IPR014362   Glu_DH       
IPR028019   DUF4508       

SUPFAM

SSF51735   SSF51735       

CDD

cd01076  NAD_bind_1_Glu_DH

ProteinModelPortal

Q2F5R4   A0A2A4JCS9   A0A0G3VFZ9   A0A194Q273   S4PE01   I4DQZ0   + More
A0A194RNK3   I4DJ42   A0A0K8TT23   E2C3C7   E9IPG3   A0A310SFZ1   A0A151X7U3   A0A2A3EPX9   A0A087ZS68   E2A6U2   A0A2J7PVJ1   A0A195FTW7   A0A154PIZ3   A0A158NNF9   W8BYK3   A0A1J1IUJ7   A0A068TJS2   A0A026WII5   A0A034VPU7   A0A0A1XFV2   A0A1I8NSV1   A0A1B6GVV3   A0A336MGF4   T1PBD9   A0A232F5M0   K7IYA3   A0A0M9AAI4   A0A195C1R0   A0A1L8EEM7   A0A158NNF3   A0A1B6EYJ5   A0A1L8E2M4   P54385-2   A0A0P8ZYJ0   A0A0Q9WUT7   A0A0R1EBF8   A0A182QQS6   A0A1W4U8M3   A0A1J1IWE9   A0A034VKL6   A0A2M4CUS3   A0A0Q9WR91   A0A3B0KCM3   A0A1I8NSU8   I5ANX6   A0A2M4CT81   A0A0C9Q076   A0A0Q9X1Y5   A0A0A1X040   A0A1A9Y0I5   A0A1B0B2Z8   T1P987   A0A1A9W5Q9   D3TQR2   A0A1A9ZSU9   A0A0K8UFK5   A0A2M3ZZI4   A0A336KTS7   A0A182R8H6   A0A1L8EER7   F5HLL7   A0A2M3YYJ1   B3M317   A0A0L0BRA3   A0A182XF30   A0A0M3QYL6   B4N9E8   B4PL48   B3P8B7   P54385   A0A1W4UJV6   B4QSD4   U5EZ79   A0A3B0JQU1   B4HG10   Q298M7   B4G4C1   A0A182LW14   B4K740   B4M0C5   A0A182PG80   A0A023ETW6   A0A0L7RDB5   B4JRQ0   Q2KQ98   A0A2M3ZZC8   A0A182IW02   A0A182V234   A0A182UC31   A0A182HVJ8   A0A182YJQ2  

PDB

6DHN     E-value=0,     Score=2021

KEGG

692939    K00261 glutamate dehydrogenase (NAD(P)+) [EC:1.4.1.3] | (RefSeq) glutamate dehydrogenase

PATHWAY

00220    Arginine biosynthesis - Bombyx mori (domestic silkworm)
00250    Alanine, aspartate and glutamate metabolism - Bombyx mori (domestic silkworm)
00471    D-Glutamine and D-glutamate metabolism - Bombyx mori (domestic silkworm)
00910    Nitrogen metabolism - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)
01200    Carbon metabolism - Bombyx mori (domestic silkworm)

GO

GO:0006520   GO:0016639   GO:0004352   GO:0000166   GO:0006116   GO:0005524   GO:0042802   GO:0005525   GO:0005739   GO:0006536   GO:0005829   GO:0005737   GO:0005759   GO:0019551   GO:0004353   GO:0006810   GO:0016021   GO:0016491   GO:0000287   GO:0003723   GO:0006270   GO:0042555   GO:0048384   GO:0055114  

Subcellular location

Mitochondrion matrix  

Length:

734

Number of predicted TMHs:

1

Exp number of AAs in TMHs:

22.53334

Exp number, first 60 AAs:

0.00334

Total prob of N-in:

0.05795

outside

1  -  697

TMhelix

698  -  720

inside

721  -  734

 

 

Pi

227.514607

Theta

178.991451

Tajima's D

0.810314

CLR

0.046095

CSRT

0.601369931503425

Interpretation

Possibly Positive selection