Gene

KWMTBOMO03267

Pre Gene Modal

BGIBMGA013607

Annotation

PREDICTED:_uncharacterized_protein_LOC101737056_[Bombyx_mori]

Location

Bomo_Chr6(+):2653887-2671104      View in EpiBrowser

Full name

Fatty acid 2-hydroxylase      

Location in the cell

PlasmaMembrane   Reliability : 2.977

CDS

ATGAAAATAATTCGTGTTTCATTTCAGAGCCGTCTAGACTGGTCGAAGCCTTTGCTGTGCCAGCTCCACGCCATAGCGCCGGACTACGAGAAATGGGTTAACAGCGCGGTCTACCGGAAATGTAGACTTTTCGCGAGCCCATTGCTCGAGAGTCTAACATATACGCCATGGTATTTCGTTCCTATGTTCTGGATACCTGTAATTTTATATCTTGGCTGGACGCAGTATTACGAGCAAGTCGCTTGTGGAGAACATTGCACAGATGCGAACATCACAACATTCCAGTACGTGTATTATATTTCATTGGGAGTGCTGTTGTGGACGATACTGGAATACTCGCTGCATCGGTGGATCTTTCACTTGGACCCGGGGTCGTCGCTCATCATGATCAAACTGCATTTCTTAATACACGGCATGCACCATAAAGTTCCCTTTGATGGTTTGCGCCAGGTGTTCCCCCCCATACCGGCTCTAGGTATAACCACAATAATATACTCAGCACTTCGGCCGTTTCTGCCCTACCCTGTGCTAGTGCTGACCGGAGGACTCATTGGCTACCTAACTTACGACATGATACATTACTATGTTCATCACGGCTCGCCAAAAGACGGAACCTATCTGTACGCGATGAAGAGATACCACTCGAACCATCATTTCCTCAATCACGACAAAGCATACGGCATAAGCTCGAAAATGTGGGACCATGTGTTCAGGACAGTTGCTCACGTAAAAAAATTAAGTTTTAGCTTACGTTGGTAG

Protein

MKIIRVSFQSRLDWSKPLLCQLHAIAPDYEKWVNSAVYRKCRLFASPLLESLTYTPWYFVPMFWIPVILYLGWTQYYEQVACGEHCTDANITTFQYVYYISLGVLLWTILEYSLHRWIFHLDPGSSLIMIKLHFLIHGMHHKVPFDGLRQVFPPIPALGITTIIYSALRPFLPYPVLVLTGGLIGYLTYDMIHYYVHHGSPKDGTYLYAMKRYHSNHHFLNHDKAYGISSKMWDHVFRTVAHVKKLSFSLRW

Description

Catalyzes stereospecific hydroxylation of free fatty acids at the C-2 position to produce (R)-2-hydroxy fatty acids, which are building blocks of sphingolipids and glycosphingolipids common in neural tissue and epidermis. Plays an essential role in the synthesis of galactosphingolipids of the myelin sheath. Responsible for the synthesis of sphingolipids and glycosphingolipids involved in the formation of epidermal lamellar bodies critical for skin permeability barrier. Participates in the synthesis of glycosphingolipids and a fraction of type II wax diesters in sebaceous gland, specifically regulating hair follicle homeostasis. Involved in the synthesis of sphingolipids of plasma membrane rafts, controlling lipid raft mobility and trafficking of raft-associated proteins.

Cofactor

Fe cation
Zn(2+)

Similarity

Belongs to the sterol desaturase family.
Belongs to the sterol desaturase family. SCS7 subfamily.
Belongs to the cytochrome b5 family.

Uniprot

H9JVP4   A0A2H1WEE9   A0A2A4JL10   A0A194Q1K1   A0A2W1BMW4   A0A0N1IFR2   + More
U4U9U4   J3JVT4   N6TSI4   A0A182VDR4   A0A182WYS7   A0A182TQS2   A0A182LHF8   A0A182Y6V3   V5I951   A0A182HYA7   A0A1Q3FNK4   A0A182RL09   A0A1Q3FPA2   A0A182KEA3   A0A1Q3FPT5   A0A182NT21   A0A182Q052   A0A182P0Q9   A0A182WFR1   A0A084VD31   A0A182IRX2   A0A182HBU5   A0A182M5D7   A0A182FLM1   A0A1S4F5S1   Q17EC2   W5JVL3   A0A2M4BSA7   A0A067RBL4   A0A1Y1KCD3   B4KND0   D6WPH5   A0A139WEB0   B4LMP7   J9JXJ3   B4J6P4   A0A2S2P225   A0A2S2QVV5   A0A0M8ZX84   A0A2A3EE22   A0A0K8ULM3   B4MR65   A0A1B0GH23   A0A3B0JSG5   A0A1B6DBJ9   B3MDQ4   B5E0J8   A0A087ZYY0   A0A2J7PSJ3   T1DSV2   A0A3Q0J8C9   A0A034W3A7   A0A0L7RGV0   A0A1L8DYG7   A0A034W2R9   A0A1I8M2E7   A0A3Q0J3B8   A0A1L8DYW2   A0A1B0AGG5   A0A0L0BVZ3   A0A1I8PZ47   B0W2P1   A0A1S3EWE3   A0A1B6IXI3   A0A2K6ER96   A0A091VKL8   A0A1B0G1H6   A0A091MQH8   A0A091PX90   A0A1A9WR42   A0A1B6GPF9   A0A1A9XFG5   A0A087QND3   F1S436   H0VSY7   A0A1J1HMU2   A0A310SW65   A0A091IFQ9   A0A1B0BAP2   A0A2Y9G4G0   A0A2P4SK20   A0A091FP89   A0A093PTC5   A0A091GNH1   B4GBP4   G5B5X0   A0A1A9V3A1   L5MC40   A0A226MG67   A0A154PQC5   A0A340XUH1   A0A3L7I082   A0A3L8SGG7  

EC Number

1.-.-.-

Pubmed

19121390    26354079    28756777    23537049    22516182    20966253    + More
25244985    24438588    26483478    17510324    20920257    23761445    24845553    28004739    17994087    18362917    19820115    18057021    15632085    23185243    25348373    25315136    26108605    21993624    21993625    29704459    30282656   

EMBL

BABH01032275    BABH01032276    BABH01032277    BABH01032278    BABH01032279    BABH01032280    + More
BABH01032281    BABH01032282    BABH01032283    ODYU01008112    SOQ51431.1    NWSH01001169    PCG72274.1    KQ459579    KPI99457.1    KZ150071    PZC74060.1    KQ460650    KPJ13123.1    KB632149    ERL89118.1    BT127352    AEE62314.1    APGK01020738    APGK01020739    KB740234    ENN81003.1    GALX01003545    JAB64921.1    APCN01004511    GFDL01005982    JAV29063.1    GFDL01005614    JAV29431.1    GFDL01005559    JAV29486.1    AXCN02001792    ATLV01011135    KE524643    KFB35875.1    JXUM01032434    KQ560955    KXJ80223.1    AXCM01002891    CH477283    EAT44857.1    ADMH02000297    ETN67075.1    GGFJ01006825    MBW55966.1    KK852611    KDR20253.1    GEZM01088641    GEZM01088640    GEZM01088639    JAV58051.1    CH933808    EDW09983.2    KQ971354    EFA06812.1    KYB26252.1    CH940648    EDW60988.1    KRF79721.1    ABLF02018471    ABLF02018472    ABLF02018473    ABLF02018474    CH916367    EDW00947.1    GGMR01010347    MBY22966.1    GGMS01012059    MBY81262.1    KQ435809    KOX72965.1    KZ288269    PBC29977.1    GDHF01024700    GDHF01009000    GDHF01005657    GDHF01003629    GDHF01000784    JAI27614.1    JAI43314.1    JAI46657.1    JAI48685.1    JAI51530.1    CH963849    EDW74604.1    AJWK01003896    AJWK01003897    OUUW01000001    SPP75671.1    GEDC01014221    JAS23077.1    CH902619    EDV36439.1    CM000071    EDY68997.2    KRT02095.1    NEVH01021928    PNF19304.1    GAMD01000065    JAB01526.1    GAKP01010337    JAC48615.1    KQ414596    KOC70085.1    GFDF01002618    JAV11466.1    GAKP01010340    GAKP01010335    GAKP01010333    JAC48617.1    GFDF01002619    JAV11465.1    JRES01001254    KNC24188.1    DS231827    EDS29349.1    GECU01016051    JAS91655.1    KL411055    KFR03013.1    CCAG010005392    KK835055    KFP79397.1    KK679659    KFQ11883.1    GECZ01005526    JAS64243.1    KL225750    KFM02737.1    AEMK02000039    AAKN02026378    AAKN02026379    CVRI01000011    CRK89267.1    KQ759870    OAD62446.1    KL218516    KFP06200.1    JXJN01011086    JXJN01011087    PPHD01041061    POI24454.1    KK719362    KFO63090.1    KL669996    KFW77475.1    KL448320    KFO82826.1    CH479181    EDW32302.1    JH168604    EHB04681.1    KB101785    ELK36174.1    MCFN01000948    OXB54287.1    KQ435037    KZC14103.1    RAZU01000121    RLQ71628.1    QUSF01000023    RLW01321.1   

Proteomes

UP000005204    UP000218220    UP000053268    UP000053240    UP000030742    UP000019118    + More
UP000075903    UP000076407    UP000075902    UP000075882    UP000076408    UP000075840    UP000075900    UP000075881    UP000075884    UP000075886    UP000075885    UP000075920    UP000030765    UP000075880    UP000069940    UP000249989    UP000075883    UP000069272    UP000008820    UP000000673    UP000027135    UP000009192    UP000007266    UP000008792    UP000007819    UP000001070    UP000053105    UP000242457    UP000007798    UP000092461    UP000268350    UP000007801    UP000001819    UP000005203    UP000235965    UP000079169    UP000053825    UP000095301    UP000092445    UP000037069    UP000095300    UP000002320    UP000081671    UP000233160    UP000053283    UP000092444    UP000091820    UP000092443    UP000053286    UP000008227    UP000005447    UP000183832    UP000054308    UP000092460    UP000248480    UP000052976    UP000053258    UP000053760    UP000008744    UP000006813    UP000078200    UP000198323    UP000076502    UP000265300    UP000273346    UP000276834   

PRIDE

H9JVP4    F1S436   

Pfam

PF05699   Dimer_Tnp_hAT        + More
PF03098   An_peroxidase
PF04116   FA_hydroxylase
PF00173   Cyt-b5

Interpro

IPR012337   RNaseH-like_sf        + More
IPR008906   HATC_C_dom       
IPR006694   Fatty_acid_hydroxylase       
IPR010255   Haem_peroxidase_sf       
IPR019791   Haem_peroxidase_animal       
IPR037120   Haem_peroxidase_sf_animal       
IPR036400   Cyt_B5-like_heme/steroid_sf       
IPR001199   Cyt_B5-like_heme/steroid-bd       
IPR018506   Cyt_B5_heme-BS       
IPR029590   Pxt       
IPR014430   Scs7       

SUPFAM

SSF48113   SSF48113        + More
SSF53098   SSF53098       
SSF55856   SSF55856       

Gene 3D

1.10.640.10     3.10.120.10    

ProteinModelPortal

H9JVP4   A0A2H1WEE9   A0A2A4JL10   A0A194Q1K1   A0A2W1BMW4   A0A0N1IFR2   + More
U4U9U4   J3JVT4   N6TSI4   A0A182VDR4   A0A182WYS7   A0A182TQS2   A0A182LHF8   A0A182Y6V3   V5I951   A0A182HYA7   A0A1Q3FNK4   A0A182RL09   A0A1Q3FPA2   A0A182KEA3   A0A1Q3FPT5   A0A182NT21   A0A182Q052   A0A182P0Q9   A0A182WFR1   A0A084VD31   A0A182IRX2   A0A182HBU5   A0A182M5D7   A0A182FLM1   A0A1S4F5S1   Q17EC2   W5JVL3   A0A2M4BSA7   A0A067RBL4   A0A1Y1KCD3   B4KND0   D6WPH5   A0A139WEB0   B4LMP7   J9JXJ3   B4J6P4   A0A2S2P225   A0A2S2QVV5   A0A0M8ZX84   A0A2A3EE22   A0A0K8ULM3   B4MR65   A0A1B0GH23   A0A3B0JSG5   A0A1B6DBJ9   B3MDQ4   B5E0J8   A0A087ZYY0   A0A2J7PSJ3   T1DSV2   A0A3Q0J8C9   A0A034W3A7   A0A0L7RGV0   A0A1L8DYG7   A0A034W2R9   A0A1I8M2E7   A0A3Q0J3B8   A0A1L8DYW2   A0A1B0AGG5   A0A0L0BVZ3   A0A1I8PZ47   B0W2P1   A0A1S3EWE3   A0A1B6IXI3   A0A2K6ER96   A0A091VKL8   A0A1B0G1H6   A0A091MQH8   A0A091PX90   A0A1A9WR42   A0A1B6GPF9   A0A1A9XFG5   A0A087QND3   F1S436   H0VSY7   A0A1J1HMU2   A0A310SW65   A0A091IFQ9   A0A1B0BAP2   A0A2Y9G4G0   A0A2P4SK20   A0A091FP89   A0A093PTC5   A0A091GNH1   B4GBP4   G5B5X0   A0A1A9V3A1   L5MC40   A0A226MG67   A0A154PQC5   A0A340XUH1   A0A3L7I082   A0A3L8SGG7  

PDB

4ZR0     E-value=9.23468e-28,     Score=305

KEGG

101737056    K19703 4-hydroxysphinganine ceramide fatty acyl 2-hydroxylase [EC:1.14.18.6] | (RefSeq) uncharacteri

GO

GO:0008610   GO:0006979   GO:0005506   GO:0020037   GO:0046983   GO:0016021   GO:0004601   GO:0016491   GO:0080132   GO:0006633   GO:0005789   GO:0046872   GO:0006631   GO:0005783   GO:0001949   GO:0044857   GO:0042634   GO:0061436   GO:0042127   GO:0006682   GO:0032287   GO:0030258   GO:0006679   GO:0032286   GO:0004702   GO:0007178   GO:0016020   GO:0005634   GO:0006270   GO:0042555   GO:0048384   GO:0055114  

PANTHER

PTHR11475:SF120  

Subcellular location

Endoplasmic reticulum membrane  

Length:

252

Number of predicted TMHs:

5

Exp number of AAs in TMHs:

81.3420400000001

Exp number, first 60 AAs:

7.45533

Total prob of N-in:

0.22462

inside

1  -  53

TMhelix

54  -  76

outside

77  -  90

TMhelix

91  -  110

inside

111  -  116

TMhelix

117  -  136

outside

137  -  150

TMhelix

151  -  168

inside

169  -  174

TMhelix

175  -  197

outside

198  -  252

 

 

Pi

165.534443

Theta

161.02436

Tajima's D

1.133842

CLR

0.279217

CSRT

0.695515224238788

Interpretation

Uncertain