SGID Silkworm Genome Informatics Database
Gene
KWMTBOMO03241
Annotation
PREDICTED:_transmembrane_protein_258_[Amyelois_transitella]
Full name
Transmembrane protein 258      
Alternative Name
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit TMEM258
Protein NEF1
Location in the cell
PlasmaMembrane   Reliability : 4.405
 

Sequence

CDS
ATGAGTCTCGAAATTGAATCAATGATCCGGTACACGTCGCCAATAAACCCGGCAGTTTTTCCACATCTAACCTTTTTGTTGCTCGGAATAGGAATATTCTTCACGGCCTGGTTCTTCGTTTACGAAGTTACAAGTACTAAAGCCTCAAGAGATATGTTCAAAGAGTTACTTTTATCATTGGTGGCCGCCTTATTTTCTGGTTTCGGAATTTTATTCTTGTTGCTATGGGTAGGGATTTATGTGTGA
Protein
MSLEIESMIRYTSPINPAVFPHLTFLLLGIGIFFTAWFFVYEVTSTKASRDMFKELLLSLVAALFSGFGILFLLLWVGIYV

Summary

Description
Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity. Involved in ER homeostasis in the colonic epithelium.
Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity (PubMed:26472760, PubMed:27974209). Involved in ER homeostasis in the colonic epithelium (By similarity).
Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.
Subunit
Component of the oligosaccharyltransferase (OST) complex. OST exists in two different complex forms which contain common core subunits RPN1, RPN2, OST48, OST4, DAD1 and TMEM258, either STT3A or STT3B as catalytic subunits, and form-specific accessory subunits (Probable). STT3A complex assembly occurs through the formation of 3 subcomplexes. Subcomplex 1 contains RPN1 and TMEM258, subcomplex 2 contains the STT3A-specific subunits STT3A, DC2/OSTC, and KCP2 as well as the core subunit OST4, and subcomplex 3 contains RPN2, DAD1, and OST48. The STT3A complex can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes (By similarity).
Component of the oligosaccharyltransferase (OST) complex. OST exists in two different complex forms which contain common core subunits RPN1, RPN2, OST48, OST4, DAD1 and TMEM258, either STT3A or STT3B as catalytic subunits, and form-specific accessory subunits (PubMed:26472760, PubMed:27974209). STT3A complex assembly occurs through the formation of 3 subcomplexes. Subcomplex 1 contains RPN1 and TMEM258, subcomplex 2 contains the STT3A-specific subunits STT3A, DC2/OSTC, and KCP2 as well as the core subunit OST4, and subcomplex 3 contains RPN2, DAD1, and OST48. The STT3A complex can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes (By similarity).
Component of the oligosaccharyltransferase (OST) complex.
Similarity
Belongs to the OST5 family.
Keywords
Acetylation   Complete proteome   Endoplasmic reticulum   Membrane   Reference proteome   Transmembrane   Transmembrane helix   Direct protein sequencing  
Feature
chain  Transmembrane protein 258
EMBL
GAIX01009083    JAA83477.1    KQ459579    KPI99479.1    JTDY01000347    KOB77592.1    + More
KZ150071    PZC74037.1    CP026252    AWP08353.1    AWP08354.1    RJVU01072345    ROI37071.1    FR912904    CDQ92180.1    KQ435724    KOX78304.1    AFYH01010909    GU589132    ADO29084.1    AYCK01006721    KQ434783    KZC04748.1    JR044273    AEY59859.1    QUSF01000061    RLV97291.1    GBSH01002862    JAG66165.1    GDAY02002675    JAV48792.1    GAAZ01002670    JAA95273.1    JU176467    GBEX01003634    AFJ51990.1    JAI10926.1    GAEP01001848    GBEW01001103    JAB52973.1    JAI09262.1    MUZQ01000123    OWK57429.1    LSYS01006880    OPJ74140.1    DQ216597    DQ216599    DQ216600    ACH45819.1    GFFW01004830    JAV39958.1    AAKN02045306    AB191468    AK145833    CH466534    BAD52442.1    BAE26683.1    EDL41344.1    CABD030079472    ADFV01079058    ADFV01079059    ADFV01079060    ADFV01079061    AEMK02000006    AQIB01079253    AHZZ02006386    AANG04000779    JSUE03012176    NDHI03003558    PNJ22062.1    PNJ22063.1    AQIA01020044    AQIA01020045    AQIA01020046    AQIA01027825    GAMS01011105    GAMR01010796    GAMQ01007611    GAMP01008697    JAB12031.1    JAB23136.1    JAB34240.1    JAB44058.1    AACZ04057293    GABF01010702    GABD01010806    GABE01003909    NBAG03000297    JAA11443.1    JAA22294.1    JAA40830.1    PNI45243.1    PNI45247.1    AK007351    AF086763    AF070661    AK291683    AP002380    CH471076    BC002750    BC015968    AB073598    AJ720522    QOIP01000011    RLU17271.1    AAPE02060815    AGTP01066774    CM004472    OCT83896.1    AABR07067758    BC077679    BC073260    AAWZ02021164    NNAY01001672    OXU23259.1   
Proteomes
UP000053268    UP000037510    UP000246464    UP000087266    UP000193380    UP000053105    + More
UP000001038    UP000265180    UP000008672    UP000221080    UP000005226    UP000261480    UP000261460    UP000261540    UP000002852    UP000192224    UP000261500    UP000265140    UP000265100    UP000265160    UP000265080    UP000028760    UP000192220    UP000076502    UP000265200    UP000005203    UP000261440    UP000276834    UP000265120    UP000261400    UP000007635    UP000242638    UP000001645    UP000197619    UP000190648    UP000189705    UP000189706    UP000005447    UP000081671    UP000233120    UP000233180    UP000001519    UP000001073    UP000252040    UP000233140    UP000008227    UP000029965    UP000002281    UP000248480    UP000265300    UP000028761    UP000233020    UP000248484    UP000233200    UP000248481    UP000245341    UP000011712    UP000233060    UP000079721    UP000248483    UP000189704    UP000245340    UP000233080    UP000233040    UP000006718    UP000233220    UP000233160    UP000261681    UP000233100    UP000248482    UP000008225    UP000002277    UP000000589    UP000005640    UP000000539    UP000279307    UP000001074    UP000005215    UP000186698    UP000002494    UP000008143    UP000261560    UP000001646    UP000215335    UP000002358   
Pfam
PF05251   Ost5        + More
PF13884   Peptidase_S74
PF13888   MRF_C2
PF05224   NDT80_PhoG
PF13887   MRF_C1
Interpro
IPR007915   TMEM258/Ost5        + More
IPR026932   MRF_C1       
IPR026933   MRF       
IPR024061   NDT80_DNA-bd_dom       
IPR030392   S74_ICA       
IPR025719   MRF_C2       
IPR008967   p53-like_TF_DNA-bd       
SUPFAM
SSF49417   SSF49417       

Ontologies

Topology

Subcellular location
Membrane  
Endoplasmic reticulum  
Length:
81
Number of predicted TMHs:
2
Exp number of AAs in TMHs:
45.43969
Exp number, first 60 AAs:
26.96057
Total prob of N-in:
0.56262
POSSIBLE N-term signal
sequence
inside
1  -  18
TMhelix
19  -  41
outside
42  -  55
TMhelix
56  -  78
inside
79  -  81
 
 

Population Genetic Test Statistics

Pi
176.838059
Theta
159.516263
Tajima's D
0.406446
CLR
26.401487
CSRT
0.485525723713814
Interpretation
Uncertain
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