Gene

KWMTBOMO03192

Pre Gene Modal

BGIBMGA013646

Annotation

PREDICTED:_iodotyrosine_dehalogenase_1_[Papilio_xuthus]

Location

Bomo_Chr6(-):1051609-1063142      View in EpiBrowser

Full name

Iodotyrosine deiodinase 1      

Alternative Name

Iodotyrosine dehalogenase 1

Location in the cell

Nuclear   Reliability : 1.943

CDS

ATGAATATTATATCGCTAGTTGTTTGGACATTGGAGAAGAACTGTTTTATCGTGTACACAGCATTAATAGTTTGTTTTATAGGCTTCACGATATTCCACAAGAACAGATTGGATATTCATTACAGGAATGTTGAAATAAAGGAAGAAAAATTGGCGCGAACCGGCCCAAAGAAAAAAGAACGTGAATTCCCTGATGATGGGGACGATGACCCAGAGTTACTCCTGCCAGCAATCCCGGAAGACACTCCGCACATACCTTACCGACCTCCGAGGAAATCCGATGAAGAAATCCTTCAGAAGTCCAGGGAATATTATGAGCTGATGGCCAAAAGGCGAACGGTCAGATGTTTTAGCGCTGAGCCTATACCTCAGGAAGTCTTGGATAATATTGTGAAGACTGCTGGCACTGCACCATCAGGCGCTCACACTGAACCCTGGACCTTTGTAGTGGTCCAAGATCCACAAATTAAGGAAGCAATCCGCGAGATTGTAGAACAAGAAGAGGAATTAAACTACACCCAGAGGATGTCCAGACAATGGGTGACTGATCTCAAACCTTTTGCCACAAAGCCAAGCAAACCTTACCTATCTGAAGCACCAGCTTTAGTTCTGGTGTTCAGACAGGCGTACTCCTGGAGATCAGATGGAAAGAAACGGATGCATTACTATAGTGAGATCAGCACGGCCATCGCAGCAGGATTTCTGCTAGCAGCTATTCAGTACTGCGGCCTAGTGGCGCTTACATCGACACCTCTAAATGCCAACGCTCGTCTCCGGGATCTCCTCTCGAGACCAGCCAATGAGAGACTGGAACTCCTTCTGCCGGTAGGAAGGCCGCACAAGGAAACAACTGTACCTGATTTAGATAGAAAGCCTTTAGAAGAGATCATGCTTAAGATCTGA

Protein

MNIISLVVWTLEKNCFIVYTALIVCFIGFTIFHKNRLDIHYRNVEIKEEKLARTGPKKKEREFPDDGDDDPELLLPAIPEDTPHIPYRPPRKSDEEILQKSREYYELMAKRRTVRCFSAEPIPQEVLDNIVKTAGTAPSGAHTEPWTFVVVQDPQIKEAIREIVEQEEELNYTQRMSRQWVTDLKPFATKPSKPYLSEAPALVLVFRQAYSWRSDGKKRMHYYSEISTAIAAGFLLAAIQYCGLVALTSTPLNANARLRDLLSRPANERLELLLPVGRPHKETTVPDLDRKPLEEIMLKI

Description

Catalyzes the oxidative NADPH-dependent deiodination of monoiodotyrosine (L-MIT) or diiodotyrosine (L-DIT). Acts during the hydrolysis of thyroglobulin to liberate iodide, which can then reenter the hormone-producing pathways. Acts more efficiently on monoiodotyrosine than on diiodotyrosine.
Catalyzes the oxidative NADPH-dependent deiodination of monoiodotyrosine (L-MIT) or diiodotyrosine (L-DIT) (PubMed:15289438, PubMed:25395621, PubMed:18434651). Acts during the hydrolysis of thyroglobulin to liberate iodide, which can then reenter the hormone-producing pathways (PubMed:18434651). Acts more efficiently on monoiodotyrosine than on diiodotyrosine (PubMed:15289438).

Catalytic Activity

2 iodide + L-tyrosine + 2 NADP(+) = 3,5-diiodo-L-tyrosine + H(+) + 2 NADPH

Cofactor

FMN

Biophysicochemical Properties

2.67 uM for L-DIT
1.35 uM for L-MIT

Subunit

Homodimer.

Similarity

Belongs to the nitroreductase family.

Keywords

Cell membrane   Complete proteome   Cytoplasmic vesicle   Flavoprotein   FMN   Membrane   NADP   Oxidoreductase   Reference proteome   Signal   Transmembrane   Transmembrane helix   3D-structure   Alternative splicing   Congenital hypothyroidism   Disease mutation   Polymorphism  

Feature

chain  Iodotyrosine deiodinase 1
splice variant  In isoform 7.
sequence variant  In TDH4; strongly reduces activity; does not respond to the increase of flavin mononucleotide concentration; dbSNP:rs121918138.

Uniprot

A0A212F7F3   A0A194Q2F6   A0A0N0PCT3   A0A2A4JJG2   E0VY32   A0A023F693   + More
A0A0A9VZ71   U6CRC6   A0A067QI15   N6TUM2   A0A1U7SH75   U4UEI0   F7G6J3   M3YG65   T2MBC4   S9WYP1   A0A1S3DTX9   A0A2Y9KZB1   Q5M8L2   A0A2J7RFY6   G1PBC7   A0A224XUI5   G3VRG0   S7NPQ6   E2R3F8   A0A1D2N124   W5NH61   A0A384BBU3   L5KFR3   A0A2U4CNL6   A0A2K6GN80   A0A2K6THK6   Q5REW1   A0A151NM03   A0A2K5DS00   H2PKM1   A0A2Y9MA64   A0A340XBK5   A0A341BND5   W4YXJ6   A0A093IFE1   A0A2K5SD20   A0A2I3GFV1   A0A2K5NU14   Q6PHW0   A0A096MYS1   A0A384CFM7   A0A091I4X7   A0A3B4UGH1   A0A2K6RRY2   A0A093R224   A0A195ES38   A0A2K6JTE7   A0A091P7U2   A0A0Q3US12   A0A2I2YAN4   A0A287BQ51   H0WUU8   A0A1D5Q4F0   A0A2R8M9R6   A0A2K6CKT5   H9GBT3   E2AVW2   A0A093K8Y2   A0A2Y9HHK8   A0A2K5YZJ4   A0A0F8AVE2   A0A2Y9ELN2   E1C0X6   A0A2K5UT53   A0A2D4HYH2   A0A091T2Q0   Q66KT6   E9IKN4   A0A3B4WUT9   A0A2K5IXZ5   A0A1L8G7Q2   A0A091W310   A0A093DQL0   A0A2R9B550   M3W0V6   A0A091G6B1   A0A2U3W7E9   A0A3B5AS70   A0A094L5D4   A0A2I3TW58   A0A2I0MTL1   F4W5M4   A0A091KIQ3   A0A091NDG1   A0A2D0SI30   A0A1A8EIP4   A0A091T8E8   A0A3P9K2U3   A0A226PUQ9  

EC Number

1.21.1.1

Pubmed

22118469    26354079    20566863    25474469    25401762    24845553    + More
23537049    17495919    24065732    23149746    20431018    21993624    21709235    16341006    27289101    23258410    22293439    14574404    15489334    14702039    15289438    24275569    25395621    18434651    24813606    25362486    22398555    30723633    17431167    21881562    20798317    25835551    15592404    21282665    27762356    22722832    17975172    16136131    23371554    21719571    17554307    24621616   

EMBL

AGBW02009879    OWR49660.1    KQ459579    KPI99518.1    KQ460434    KPJ14760.1    + More
NWSH01001314    PCG71734.1    DS235843    EEB18288.1    GBBI01001965    JAC16747.1    GBHO01044126    JAF99477.1    HAAF01001225    CCP73051.1    KK853366    KDR08112.1    APGK01008157    APGK01018039    KB740064    KB737617    ENN81768.1    ENN82987.1    KB632132    ERL89011.1    AEYP01034373    HAAD01003154    CDG69386.1    KB017067    EPY80644.1    AAMC01122984    BC087975    AAH87975.1    NEVH01004408    PNF39738.1    AAPE02047741    GFTR01004815    JAW11611.1    AEFK01173337    AEFK01173338    AEFK01173339    AEFK01173340    KE164616    EPQ19221.1    AAEX03000269    LJIJ01000310    ODM98938.1    AHAT01015800    KB030829    ELK09338.1    CR857405    AKHW03002600    KYO37699.1    ABGA01293022    ABGA01293023    ABGA01293024    ABGA01293025    NDHI03003369    PNJ78989.1    AAGJ04097708    KK586712    KFV97493.1    ADFV01166569    ADFV01166570    ADFV01166571    AY259176    AY259177    AY424901    AY424902    AY957659    AY957660    AY957661    AL031010    BC056253    AK129950    AHZZ02025568    KL218240    KFP03272.1    KL425797    KFW90227.1    KQ981993    KYN31043.1    KK653785    KFQ03461.1    LMAW01002567    KQK79802.1    CABD030048437    AEMK02000001    DQIR01168408    DQIR01293609    HDB23885.1    AAQR03140332    JSUE03031928    AAWZ02022304    GL443213    EFN62384.1    KL206904    KFV86844.1    KQ040945    KKF32702.1    AADN05000003    AQIA01050252    IACK01070805    LAA77003.1    KK495778    KFQ65492.1    BC078565    AAH78565.1    GL764021    EFZ18868.1    CM004474    OCT79893.1    KK734675    KFR10017.1    KL449365    KFV04446.1    AJFE02036324    AJFE02036325    AANG04000169    KL447083    KFO69732.1    KL272168    KFZ64647.1    AACZ04040565    NBAG03000056    PNI93106.1    AKCR02000002    PKK33016.1    GL887655    EGI70521.1    KK741999    KFP39055.1    KK845149    KFP86924.1    HAEB01000528    SBQ46976.1    KK442700    KFQ70719.1    AWGT02000010    OXB83761.1   

Proteomes

UP000007151    UP000053268    UP000053240    UP000218220    UP000009046    UP000027135    + More
UP000019118    UP000189705    UP000030742    UP000002280    UP000000715    UP000079169    UP000248482    UP000008143    UP000235965    UP000001074    UP000007648    UP000002254    UP000094527    UP000018468    UP000261681    UP000010552    UP000245320    UP000233160    UP000233220    UP000001595    UP000050525    UP000233020    UP000248483    UP000265300    UP000252040    UP000007110    UP000233040    UP000001073    UP000233060    UP000005640    UP000028761    UP000261680    UP000291021    UP000054308    UP000261420    UP000233200    UP000078541    UP000233180    UP000051836    UP000001519    UP000008227    UP000005225    UP000006718    UP000008225    UP000233120    UP000001646    UP000000311    UP000053584    UP000248481    UP000233140    UP000248484    UP000000539    UP000233100    UP000261360    UP000233080    UP000186698    UP000053605    UP000240080    UP000011712    UP000053760    UP000245340    UP000261400    UP000002277    UP000053872    UP000007755    UP000221080    UP000265180    UP000198419   

PRIDE

Q5REW1    Q6PHW0   

Pfam

PF00881   Nitroreductase

Interpro

IPR029479   Nitroreductase        + More
IPR000415   Nitroreductase-like       

SUPFAM

SSF55469   SSF55469       

Gene 3D

3.40.109.10    

ProteinModelPortal

A0A212F7F3   A0A194Q2F6   A0A0N0PCT3   A0A2A4JJG2   E0VY32   A0A023F693   + More
A0A0A9VZ71   U6CRC6   A0A067QI15   N6TUM2   A0A1U7SH75   U4UEI0   F7G6J3   M3YG65   T2MBC4   S9WYP1   A0A1S3DTX9   A0A2Y9KZB1   Q5M8L2   A0A2J7RFY6   G1PBC7   A0A224XUI5   G3VRG0   S7NPQ6   E2R3F8   A0A1D2N124   W5NH61   A0A384BBU3   L5KFR3   A0A2U4CNL6   A0A2K6GN80   A0A2K6THK6   Q5REW1   A0A151NM03   A0A2K5DS00   H2PKM1   A0A2Y9MA64   A0A340XBK5   A0A341BND5   W4YXJ6   A0A093IFE1   A0A2K5SD20   A0A2I3GFV1   A0A2K5NU14   Q6PHW0   A0A096MYS1   A0A384CFM7   A0A091I4X7   A0A3B4UGH1   A0A2K6RRY2   A0A093R224   A0A195ES38   A0A2K6JTE7   A0A091P7U2   A0A0Q3US12   A0A2I2YAN4   A0A287BQ51   H0WUU8   A0A1D5Q4F0   A0A2R8M9R6   A0A2K6CKT5   H9GBT3   E2AVW2   A0A093K8Y2   A0A2Y9HHK8   A0A2K5YZJ4   A0A0F8AVE2   A0A2Y9ELN2   E1C0X6   A0A2K5UT53   A0A2D4HYH2   A0A091T2Q0   Q66KT6   E9IKN4   A0A3B4WUT9   A0A2K5IXZ5   A0A1L8G7Q2   A0A091W310   A0A093DQL0   A0A2R9B550   M3W0V6   A0A091G6B1   A0A2U3W7E9   A0A3B5AS70   A0A094L5D4   A0A2I3TW58   A0A2I0MTL1   F4W5M4   A0A091KIQ3   A0A091NDG1   A0A2D0SI30   A0A1A8EIP4   A0A091T8E8   A0A3P9K2U3   A0A226PUQ9  

PDB

4TTC     E-value=1.0992e-64,     Score=624

KEGG

101741567    K17231 iodotyrosine deiodinase [EC:1.21.1.1] | (RefSeq) iodotyrosine dehalogenase 1

GO

GO:0016491   GO:0016021   GO:0005887   GO:0004447   GO:0030659   GO:0006570   GO:0042403   GO:0010181   GO:0005654   GO:0055114   GO:0005886   GO:0006590   GO:0004672   GO:0006468   GO:0046982   GO:0005615   GO:0005515   GO:0045211   GO:0006281   GO:0048384  

Subcellular location

Cell membrane  
Cytoplasmic vesicle membrane  

Length:

300

Number of predicted TMHs:

2

Exp number of AAs in TMHs:

41.42152

Exp number, first 60 AAs:

18.88122

Total prob of N-in:

0.01259

POSSIBLE N-term signal

sequence

outside

1  -  14

TMhelix

15  -  32

inside

33  -  225

TMhelix

226  -  248

outside

249  -  300

 

 

Pi

177.661443

Theta

141.512551

Tajima's D

-1.189524

CLR

0.544196

CSRT

0.104844757762112

Interpretation

Uncertain