SGID Silkworm Genome Informatics Database
Gene
KWMTBOMO03182
Pre Gene Modal
BGIBMGA013453
Annotation
cyclin-dependent_kinase_2_[Bombyx_mori]
Full name
Cyclin-dependent kinase 2      
Alternative Name
Cell division protein kinase 2
p33 protein kinase
Location in the cell
Cytoplasmic   Reliability : 2.383
 

Sequence

CDS
ATGGAGAATTTTTCTACTGTAGAAAAAATAGGGGAAGGAACATATGGTGTAGTTTACAAAGCCAAAGACAGGGTCACCGGACAAGAAATCGCGTTGAAGAAAATCAAACTTGAAAATGAACCTGAGGGTGTACCATCAACAGCCTTACGGGAGATCTCAGTGCTGCGAGAACTTCGACATCCAGCAGTTGTACGGCTCCTTGATGTTATGCTGGCATCTTCAGACAGTAAACTGTTTCTTGTCTTTGAATATTTGAATATGGATCTCAAAAGACTCATGGATCTGACGAAGGGACCACTACCTATTGATTTAGTTAAGAGTTATTTACGACAGTTACTGGAAGGTGTTGCGTACTGTCACGCCCAACGTGTTCTCCACAGAGACCTGAAGCCCCAGAACCTACTAATAGACGAGGAAGGTCACATTAAATTGGCCGATTTCGGTTTGGCTCGAGCTTTTGGTATCCCTGTGCGTGCTTACACACATGAGGTGGTCACACTATGGTACCGTGCACCAGAAATCCTTCTTGGGGCTAAGTTCTACTCAACCGCCGTTGATGTTTGGAGCTTAGCCTGTATTTTTGCAGAGATGGCAAGCGGTCGCACTCTTTTCCCCGGTGACAGTGAAATAGACCAGCTATTCCGCGTATTCCGAGCGCTGGGTACACCAGGCGAGGCACTATGGCCGGCAGCGAGAAGACTTCCTGACTTCCGAGCCGCCTTCCCGCGATGGCCTGCCCGACCTGCCCGAACATTACTACCAGCAGGACTCCGAGCGCACAGTTCAGCGGCAGCGTTGTTTGAGGCCATGCTGCGTTATGAACCTGAGACTCGAATTCCTGCCCGAGCTGCTCTTACGCATCCTTACTTGGCTGATGCGACTCTGGTGCCGCCTCCTCTACATCCTTAA
Protein
MENFSTVEKIGEGTYGVVYKAKDRVTGQEIALKKIKLENEPEGVPSTALREISVLRELRHPAVVRLLDVMLASSDSKLFLVFEYLNMDLKRLMDLTKGPLPIDLVKSYLRQLLEGVAYCHAQRVLHRDLKPQNLLIDEEGHIKLADFGLARAFGIPVRAYTHEVVTLWYRAPEILLGAKFYSTAVDVWSLACIFAEMASGRTLFPGDSEIDQLFRVFRALGTPGEALWPAARRLPDFRAAFPRWPARPARTLLPAGLRAHSSAAALFEAMLRYEPETRIPARAALTHPYLADATLVPPPLHP

Summary

Description
Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in human embryonic stem cells (hESCs). Activity of CDK2 is maximal during S phase and G2; activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. Phosphorylates CABLES1 (By similarity). Cyclin E/CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC. Involved in G1-S phase DNA damage checkpoint that prevents cells with damaged DNA from initiating mitosis; regulates homologous recombination-dependent repair by phosphorylating BRCA2, this phosphorylation is low in S phase when recombination is active, but increases as cells progress towards mitosis. In response to DNA damage, double-strand break repair by homologous recombination a reduction of CDK2-mediated BRCA2 phosphorylation. Phosphorylation of RB1 disturbs its interaction with E2F1. NPM1 phosphorylation by cyclin E/CDK2 promotes its dissociates from unduplicated centrosomes, thus initiating centrosome duplication. Cyclin E/CDK2-mediated phosphorylation of NPAT at G1-S transition and until prophase stimulates the NPAT-mediated activation of histone gene transcription during S phase. Required for vitamin D-mediated growth inhibition by being itself inactivated. Involved in the nitric oxide- (NO) mediated signaling in a nitrosylation/activation-dependent manner. USP37 is activated by phosphorylation and thus triggers G1-S transition. CTNNB1 phosphorylation regulates insulin internalization. Phosphorylates FOXP3 and negatively regulates its transcriptional activity and protein stability (PubMed:23853094). Phosphorylates CDK2AP2 (By similarity).
Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in human embryonic stem cells (hESCs). Activity of CDK2 is maximal during S phase and G2; activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. Phosphorylates CABLES1 (By similarity). Cyclin E/CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC. Involved in G1-S phase DNA damage checkpoint that prevents cells with damaged DNA from initiating mitosis; regulates homologous recombination-dependent repair by phosphorylating BRCA2, this phosphorylation is low in S phase when recombination is active, but increases as cells progress towards mitosis. In response to DNA damage, double-strand break repair by homologous recombination a reduction of CDK2-mediated BRCA2 phosphorylation. Phosphorylation of RB1 disturbs its interaction with E2F1. NPM1 phosphorylation by cyclin E/CDK2 promotes its dissociates from unduplicated centrosomes, thus initiating centrosome duplication. Cyclin E/CDK2-mediated phosphorylation of NPAT at G1-S transition and until prophase stimulates the NPAT-mediated activation of histone gene transcription during S phase. Required for vitamin D-mediated growth inhibition by being itself inactivated. Involved in the nitric oxide- (NO) mediated signaling in a nitrosylation/activation-dependent manner. USP37 is activated by phosphorylation and thus triggers G1-S transition. CTNNB1 phosphorylation regulates insulin internalization. Phosphorylates FOXP3 and negatively regulates its transcriptional activity and protein stability (By similarity). Phosphorylates CDK2AP2 (By similarity).
Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in human embryonic stem cells (hESCs). Activity of CDK2 is maximal during S phase and G2; activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. Phosphorylates CABLES1 (By similarity). Cyclin E/CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC. Involved in G1-S phase DNA damage checkpoint that prevents cells with damaged DNA from initiating mitosis; regulates homologous recombination-dependent repair by phosphorylating BRCA2, this phosphorylation is low in S phase when recombination is active, but increases as cells progress towards mitosis. In response to DNA damage, double-strand break repair by homologous recombination a reduction of CDK2-mediated BRCA2 phosphorylation. Phosphorylation of RB1 disturbs its interaction with E2F1. NPM1 phosphorylation by cyclin E/CDK2 promotes its dissociates from unduplicated centrosomes, thus initiating centrosome duplication. Cyclin E/CDK2-mediated phosphorylation of NPAT at G1-S transition and until prophase stimulates the NPAT-mediated activation of histone gene transcription during S phase. Required for vitamin D-mediated growth inhibition by being itself inactivated. Involved in the nitric oxide- (NO) mediated signaling in a nitrosylation/activation-dependent manner. USP37 is activated by phosphorylation and thus triggers G1-S transition. CTNNB1 phosphorylation regulates insulin internalization. Phosphorylates FOXP3 and negatively regulates its transcriptional activity and protein stability (By similarity). Phosphorylates CDK2AP2 (PubMed:12944431).
Catalytic Activity
ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]
ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]
Cofactor
Mg(2+)
Subunit
Found in a complex with CABLES1, CCNA1 and CCNE1. Interacts with CABLES1 (PubMed:11585773). Interacts with UHRF2. Part of a complex consisting of UHRF2, CDK2 and CCNE1. Interacts with the Speedy/Ringo proteins SPDYA and SPDYC. Interaction with SPDYA promotes kinase activation via a conformation change that alleviates obstruction of the substrate-binding cleft by the T-loop. Found in a complex with both SPDYA and CDKN1B/KIP1. Binds to RB1 and CDK7. Binding to CDKN1A (p21) leads to CDK2/cyclin E inactivation at the G1-S phase DNA damage checkpoint, thereby arresting cells at the G1-S transition during DNA repair. Associated with PTPN6 and beta-catenin/CTNNB1. Interacts with CACUL1. May interact with CEP63. Interacts with ANKRD17 (By similarity). Interacts with CEBPA (when phosphorylated) (PubMed:15107404). Forms a ternary complex with CCNA2 and CDKN1B; CDKN1B inhibits the kinase activity of CDK2 through conformational rearrangements. Interacts with cyclins A, B1, B3, D, or E. Interacts with CDK2AP2 (By similarity).
Found in a complex with CABLES1, CCNA1 and CCNE1. Interacts with CABLES1 (By similarity). Interacts with UHRF2. Part of a complex consisting of UHRF2, CDK2 and CCNE1. Interacts with the Speedy/Ringo proteins SPDYA and SPDYC. Interaction with SPDYA promotes kinase activation via a conformation change that alleviates obstruction of the substrate-binding cleft by the T-loop. Found in a complex with both SPDYA and CDKN1B/KIP1. Binds to RB1 (PubMed:10542199). Binds to CDK7. Binding to CDKN1A (p21) leads to CDK2/cyclin E inactivation at the G1-S phase DNA damage checkpoint, thereby arresting cells at the G1-S transition during DNA repair. Associated with PTPN6 and beta-catenin/CTNNB1. Interacts with CACUL1. May interact with CEP63. Interacts with ANKRD17. Interacts with CEBPA (when phosphorylated). Forms a ternary complex with CCNA2 and CDKN1B; CDKN1B inhibits the kinase activity of CDK2 through conformational rearrangements. Interacts with cyclins A, B1, B3, D, or E. Interacts with CDK2AP2 (By similarity).
Found in a complex with CABLES1, CCNA1 and CCNE1. Interacts with CABLES1 (By similarity). Interacts with UHRF2. Part of a complex consisting of UHRF2, CDK2 and CCNE1. Interacts with the Speedy/Ringo proteins SPDYA and SPDYC. Interaction with SPDYA promotes kinase activation via a conformation change that alleviates obstruction of the substrate-binding cleft by the T-loop. Found in a complex with both SPDYA and CDKN1B/KIP1. Binds to RB1 and CDK7. Binding to CDKN1A (p21) leads to CDK2/cyclin E inactivation at the G1-S phase DNA damage checkpoint, thereby arresting cells at the G1-S transition during DNA repair. Associated with PTPN6 and beta-catenin/CTNNB1. Interacts with CACUL1. May interact with CEP63. Interacts with ANKRD17. Interacts with CEBPA (when phosphorylated). Forms a ternary complex with CCNA2 and CDKN1B; CDKN1B inhibits the kinase activity of CDK2 through conformational rearrangements. Interacts with cyclins A, B1, B3, D, or E. Interacts with CDK2AP2 (By similarity).
Found in a complex with CABLES1, CCNA1 and CCNE1. Interacts with CABLES1 (By similarity). Interacts with UHRF2. Part of a complex consisting of UHRF2, CDK2 and CCNE1. Interacts with the Speedy/Ringo proteins SPDYA and SPDYC (PubMed:15611625). Interaction with SPDYA promotes kinase activation via a conformation change that alleviates obstruction of the substrate-binding cleft by the T-loop (PubMed:28666995). Found in a complex with both SPDYA and CDKN1B/KIP1 (PubMed:12972555, PubMed:28666995). Binds to RB1 and CDK7. Binding to CDKN1A (p21) leads to CDK2/cyclin E inactivation at the G1-S phase DNA damage checkpoint, thereby arresting cells at the G1-S transition during DNA repair. Associated with PTPN6 and beta-catenin/CTNNB1. Interacts with CACUL1. May interact with CEP63. Interacts with ANKRD17. Interacts with CEBPA (when phosphorylated) (PubMed:15107404). Forms a ternary complex with CCNA2 and CDKN1B; CDKN1B inhibits the kinase activity of CDK2 through conformational rearrangements (PubMed:8684460). Interacts with cyclins A, B1, B3, D, or E (PubMed:10499802, PubMed:10884347, PubMed:12185076, PubMed:23781148). Interacts with CDK2AP2 (PubMed:23781148).
Similarity
Belongs to the protein kinase superfamily.
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Keywords
Acetylation   Alternative splicing   ATP-binding   Cell cycle   Cell division   Complete proteome   Cytoplasm   Cytoskeleton   DNA damage   DNA repair   Endosome   Kinase   Magnesium   Meiosis   Metal-binding   Mitosis   Nucleotide-binding   Nucleus   Phosphoprotein   Reference proteome   Transferase   3D-structure   Polymorphism   S-nitrosylation  
Feature
chain  Cyclin-dependent kinase 2
splice variant  In isoform CDK2-alpha.
sequence variant  In dbSNP:rs3087335.
EC Number
2.7.11.22
Pubmed
19121390    23622113    28756777    22118469    26227816    9215903    + More
9705226    21993624    23254933    23149746    23836398    19892987    16341006    17975172    24813606    20010809    15489334    11585773    11733001    14561402    12923533    15107404    17942597    21183079    23853094    15057822    15632090    10349636    11042159    11076861    11217851    12466851    12040188    16141073    23385571    7862443    8673024    10542199    21709235    20798317    25243066    11506705    21993625    22751099    19340603    16305752    30297745    22398555    22722832    25362486    22002653    25319552    16136131    11181995    1714386    1653904    1717994    14702039    16541075    1396589    9030781    10499802    11051553    10995386    10995387    10884347    11113184    12185076    11980914    12839962    12944431    12972555    15178429    14597612    15611625    15800615    17373709    18691976    18372919    19829063    19369195    19690332    19608861    20147522    20079829    20360007    20935635    20195506    19966300    21269460    21406398    21262353    21684737    21596315    21319273    19445729    19238148    19561645    20713526    21517772    22814378    23781148    8510751    7630397    8601310    8917641    8684460    8756328    8610110    9334743    9677190    16325401    17570665    17495531    17095507    17937404    18656911    21565702    28666995    17344846    23711367    21347285    15592404    17635936    30382153    8280171    28562605    24621616    21881562   
EMBL
BABH01037322    JN833622    AFB82433.1    ODYU01001044    SOQ36702.1    GAIX01003535    + More
JAA89025.1    RSAL01000053    RVE50053.1    KZ150187    PZC72439.1    NWSH01000543    PCG75764.1    AGBW02013968    OWR42504.1    JTDY01000669    KOB76276.1    KQ435789    KOX74501.1    AFYH01001758    AFYH01001759    AJ224917    CAA12223.1    KZ288357    PBC27130.1    KQ760761    OAD59142.1    AAPE02026874    KE163877    EPQ14231.1    KB203827    ESO82954.1    KQ414657    KOC65758.1    KQ434850    KZC08564.1    AAGJ04171849    KB016734    EQB77761.1    AEMK02000033    JX967576    AGR86101.1    AAEX03006916    AANG04003655    ACTA01178059    GL192457    EFB28834.1    AAQR03076828    AAQR03076829    U63337    AJ223732    AJ223733    BC005654    AC098012    AC141508    BC061832    CH474104    AAH61832.1    EDL84822.1    AK152922    CH466578    BAE31597.1    EDL24600.1    KB364768    ELV12032.1    D28753    D63162    AEFK01203592    GL440609    EFN65646.1    AGTP01085299    AGTP01085300    JH431734    GAMT01001273    GAMS01008093    GAMQ01007798    JAB10588.1    JAB15043.1    JAB34053.1    AJ223949    AAKN02014842    JH169329    GEBF01002266    EHB06556.1    JAO01367.1    JH882551    ELR48983.1    FJ422550    ACJ53943.1    LWLT01000005    EF035041    ABK34941.1    BT020790    BC150026    BFAA01003391    GCB70959.1    CABD030083632    AQIB01023022    AJFE02087845    AHZZ02002258    ABGA01042610    ABGA01042611    NDHI03003457    PNJ44889.1    AQIA01011136    CM001286    EHH66383.1    JU320958    JU473899    JV045857    CM001263    AFE64714.1    AFH30703.1    AFI35928.1    EHH20842.1    AACZ04012961    GABC01007850    GABF01000440    GABD01001183    GABE01004554    NBAG03000092    JAA03488.1    JAA21705.1    JAA31917.1    JAA40185.1    PNI90081.1    CH471054    EAW96860.1    X61622    X62071    M68520    AB012305    BT006821    AF512553    AK291941    AC025162    AC034102    BC003065    ADTU01020711    ADTU01020712    AADN05000742    EF182713    ABM55710.1    KQ981689    KYN37898.1    RCHS01000860    RMX56356.1    D17350    AANG04004184    MCFN01001318    OXB53259.1    AWGT02008165    OXB56521.1    KQ981010    KYN10338.1    AEMK02000080    KQ976593    KYM79677.1    BEZZ01000169    GCC27553.1    AAWZ02011909   
Pfam
PF00069   Pkinase        + More
PF15490   Ten1_2
Interpro
IPR000719   Prot_kinase_dom        + More
IPR017441   Protein_kinase_ATP_BS       
IPR008271   Ser/Thr_kinase_AS       
IPR011009   Kinase-like_dom_sf       
IPR029146   Ten1_animal_plant       
SUPFAM
SSF56112   SSF56112       

Ontologies

Topology

Subcellular location
Cytoplasm   Localized at the centrosomes in late G2 phase after separation of the centrosomes but before the start of prophase. Nuclear-cytoplasmic trafficking is mediated during the inhibition by 1,25-(OH)(2)D(3) (By similarity).   With evidence from 2 publications.
Cytoskeleton   Localized at the centrosomes in late G2 phase after separation of the centrosomes but before the start of prophase. Nuclear-cytoplasmic trafficking is mediated during the inhibition by 1,25-(OH)(2)D(3) (By similarity).   With evidence from 2 publications.
Microtubule organizing center   Localized at the centrosomes in late G2 phase after separation of the centrosomes but before the start of prophase. Nuclear-cytoplasmic trafficking is mediated during the inhibition by 1,25-(OH)(2)D(3) (By similarity).   With evidence from 2 publications.
Centrosome   Localized at the centrosomes in late G2 phase after separation of the centrosomes but before the start of prophase. Nuclear-cytoplasmic trafficking is mediated during the inhibition by 1,25-(OH)(2)D(3) (By similarity).   With evidence from 2 publications.
Nucleus   Localized at the centrosomes in late G2 phase after separation of the centrosomes but before the start of prophase. Nuclear-cytoplasmic trafficking is mediated during the inhibition by 1,25-(OH)(2)D(3) (By similarity).   With evidence from 2 publications.
Cajal body   Localized at the centrosomes in late G2 phase after separation of the centrosomes but before the start of prophase. Nuclear-cytoplasmic trafficking is mediated during the inhibition by 1,25-(OH)(2)D(3) (By similarity).   With evidence from 2 publications.
Endosome   Localized at the centrosomes in late G2 phase after separation of the centrosomes but before the start of prophase. Nuclear-cytoplasmic trafficking is mediated during the inhibition by 1,25-(OH)(2)D(3) (By similarity).   With evidence from 2 publications.
Length:
302
Number of predicted TMHs:
0
Exp number of AAs in TMHs:
0.0643299999999999
Exp number, first 60 AAs:
1e-05
Total prob of N-in:
0.02863
outside
1  -  302
 
 

Population Genetic Test Statistics

Pi
40.983936
Theta
33.996669
Tajima's D
-2.236398
CLR
1.164567
CSRT
0.00454977251137443
Interpretation
Uncertain

Multiple alignment of Orthologues

 
 

Gene Tree

 
 
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