Gene

KWMTBOMO03093  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA005810

Annotation

PREDICTED:_1?2-dihydroxy-3-keto-5-methylthiopentene_dioxygenase-like_[Amyelois_transitella]

Location

Bomo_Chr5(-):17864200-17867603      View in EpiBrowser

Full name

1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase      

Alternative Name

Acireductone dioxygenase (Fe(2+)-requiring)

Location in the cell

Cytoplasmic   Reliability : 3.818

CDS

ATGGTACAAGCATGGTACATGGATAGTGAAACAACGGACCAGCGGCTGCAACACCATAAAAATCCTCCAGAATATATTTCCCTGGATGATTTATTCTCTAAAACGGGGGTTGAATATTTTAAAATAAACATAGACACATATAAAACTGATGGAGTACTAGAAAAACTTAAGAATGAACGAGGTTACACATATGAAGATGAGATGGTCTGTTCCAAGGAATGTCTTCCGAATTATGAGGAGAAGATAAAATCGTTTTACACTGAGCACTTGCATACGGATGAGGAAATTAGGTTTATCCTAGACGGCTCTGGTTACTTTGACGTGAGAGATGGTGAGGATCAATGGATCCGTATTGCCGTGTCTAAGGGAGACATGCTGGTCATCCCGAGCGGTATCTACCACAGGTTTACCTTGGACACCAATAACTACATAAAAGCGAAACGTTTCTTCATTGGTGAGCCGGTTTGGTTGCCTTACAACAGGCCGGCCGATGAGATGCCTTGCCGGAAGGACTACGTTGAGAAACTACAGAATGGATTCTCAGTGGCCGCATAA

Protein

MVQAWYMDSETTDQRLQHHKNPPEYISLDDLFSKTGVEYFKINIDTYKTDGVLEKLKNERGYTYEDEMVCSKECLPNYEEKIKSFYTEHLHTDEEIRFILDGSGYFDVRDGEDQWIRIAVSKGDMLVIPSGIYHRFTLDTNNYIKAKRFFIGEPVWLPYNRPADEMPCRKDYVEKLQNGFSVAA

Description

Catalyzes the formation of formate and 2-keto-4-methylthiobutyrate (KMTB) from 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).

Catalytic Activity

1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + O2 = 4-methylsulfanyl-2-oxobutanoate + formate + 2 H(+)

Cofactor

Fe cation

Similarity

Belongs to the acireductone dioxygenase (ARD) family.
Belongs to the ATP:guanido phosphotransferase family.

Keywords

Amino-acid biosynthesis   Complete proteome   Cytoplasm   Dioxygenase   Iron   Metal-binding   Methionine biosynthesis   Nucleus   Oxidoreductase   Reference proteome  

Feature

chain  1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase

Uniprot

H9J8G8   A0A2A4K8L0   A0A2H1VUW4   A0A212FID1   A0A0N0PBR1   A0A194PQ97   + More
A0A1Q3FGT6   A0A0L7QU20   A0A088AS00   E2ATE3   A0A195EKJ9   E9ILG6   A0A0N0BK05   A0A023EJ12   A0A154PF83   A0A2A3EEP1   A0A023EIH8   B0XKA4   A0A023EH95   A0A158NJM9   U5EVG3   Q0IFH8   A0A151X6J6   A0A195BFV5   B0WLS7   A0A1L8EB68   D6WC15   A0A151IG80   A0A182JI12   A0A232F8M7   A0A084VCA3   F4W3U3   A0A182LJ71   Q7Q6X6   A0A182XMZ5   A0A182YRS4   A0A182UBX4   A0A2M3Z803   A0A182LW86   A0A1I8P6L2   A0A182UQ92   A0A182HWV2   A0A1I8MGE8   A0A2M4C0U9   A0A182FDM5   A0A182RRV6   A0A2M4AK19   A0A182NGT7   A0A1L8DC72   A0A2M3Z884   A0A2M4C0W1   A0A182KCJ4   A0A182WB23   W5JVE7   A0A182PHJ8   B4PEG5   A0A182QUH6   A0A069DNU6   V5GRX8   F3YDM3   Q6AWN0   A0A1W4UUT0   A0A195FMX8   B3NCM6   T1DP42   S4NX86   A0A034WT85   A0A0C9PSL3   B4QP87   A0A067QFY6   A0A1W4XFG1   R4G8L3   A0A0P4VVG9   R4G4H3   A0A1J1IH66   K7IUI2   R4UW56   A0A026VTR3   A0A182HA72   E0W481   A0A0K8TQU2   A0A224XZD1   A0A1A9ZF26   A0A023F9B7   A0A1I8NBE5   B4LB52   A0A336MGM1   B4J0Y0   A0A1Y1NHT5   A0A336K6P2   B4L9H0   A0A0A1WHA6   A0A2R7WU76   B3M5D1   A0A0L0C741   B4MLV1   Q2LZI9   A0A2M4AL13   A0A0A9WLE1   A0A0M4EZ24  

EC Number

1.13.11.54

Pubmed

19121390    22118469    26354079    20798317    21282665    24945155    + More
21347285    17510324    18362917    19820115    28648823    24438588    21719571    20966253    12364791    14747013    17210077    25244985    25315136    20920257    23761445    17994087    17550304    26334808    10731132    12537572    25037729    23622113    25348373    22936249    24845553    27129103    20075255    24508170    26483478    20566863    26369729    25474469    28004739    25830018    26108605    15632085    25401762   

EMBL

BABH01027394    NWSH01000041    PCG80336.1    ODYU01004415    SOQ44262.1    AGBW02008399    + More
OWR53481.1    KQ460847    KPJ11981.1    KQ459597    KPI95138.1    GFDL01008268    JAV26777.1    KQ414736    KOC62120.1    GL442548    EFN63310.1    KQ978782    KYN28394.1    GL764076    EFZ18588.1    KQ435710    KOX79884.1    GAPW01004856    GAPW01004854    JAC08742.1    JAC08744.1    KQ434892    KZC10546.1    KZ288271    PBC29742.1    GAPW01004853    JAC08745.1    DS233762    EDS31503.1    GAPW01004855    JAC08743.1    ADTU01018077    GANO01001891    JAB57980.1    CH477317    EAT43736.1    KQ982482    KYQ55949.1    KQ976490    KYM83465.1    DS231990    EDS30655.1    GFDG01002789    JAV16010.1    KQ971309    EEZ99144.1    KQ977735    KYN00245.1    NNAY01000680    OXU27015.1    ATLV01010627    KE524589    KFB35597.1    GL887459    EGI71129.1    AAAB01008960    EAA11720.3    GGFM01003869    MBW24620.1    AXCM01009801    APCN01001541    GGFJ01009791    MBW58932.1    GGFK01007815    MBW41136.1    GFDF01010023    JAV04061.1    GGFM01003897    MBW24648.1    GGFJ01009792    MBW58933.1    ADMH02000325    ETN66945.1    CM000159    EDW94031.1    AXCN02000366    GBGD01003151    JAC85738.1    GALX01005488    JAB62978.1    BT126384    AEC46900.1    AE014296    BT015218    KQ981424    KYN41818.1    CH954178    EDV51323.1    GAMD01002280    JAA99310.1    GAIX01009174    JAA83386.1    GAKP01001400    JAC57552.1    GBYB01004328    JAG74095.1    CM000363    CM002912    EDX09985.1    KMY98867.1    KK853557    KDR06747.1    ACPB03015200    GAHY01000772    JAA76738.1    GDKW01000556    JAI56039.1    GAHY01000771    JAA76739.1    CVRI01000051    CRK99563.1    AAZX01002638    KC571972    AGM32471.1    KK107928    EZA47163.1    JXUM01122182    KQ566576    KXJ70011.1    DS235886    GDAI01001328    JAI16275.1    GFTR01002967    JAW13459.1    GBBI01001204    JAC17508.1    CH940647    EDW68616.2    UFQS01001030    UFQT01001030    SSX08635.1    SSX28551.1    CH916366    EDV95801.1    GEZM01001928    JAV97453.1    UFQS01000158    UFQT01000158    SSX00646.1    SSX21026.1    CH933816    EDW17345.1    GBXI01016524    GBXI01005890    JAC97767.1    JAD08402.1    KK855557    PTY23117.1    CH902618    EDV40636.1    JRES01000919    KNC27254.1    CH963847    EDW73162.1    CH379069    GGFK01008116    MBW41437.1    GBHO01035367    GBHO01035363    GBRD01014815    GBRD01014814    JAG08237.1    JAG08241.1    JAG51011.1    CP012525    ALC43557.1    ALC43998.1   

Proteomes

UP000005204    UP000218220    UP000007151    UP000053240    UP000053268    UP000053825    + More
UP000005203    UP000000311    UP000078492    UP000053105    UP000076502    UP000242457    UP000002320    UP000005205    UP000008820    UP000075809    UP000078540    UP000007266    UP000078542    UP000075880    UP000215335    UP000030765    UP000007755    UP000075882    UP000007062    UP000076407    UP000076408    UP000075902    UP000075883    UP000095300    UP000075903    UP000075840    UP000095301    UP000069272    UP000075900    UP000075884    UP000075881    UP000075920    UP000000673    UP000075885    UP000002282    UP000075886    UP000000803    UP000192221    UP000078541    UP000008711    UP000000304    UP000027135    UP000192223    UP000015103    UP000183832    UP000002358    UP000053097    UP000069940    UP000249989    UP000009046    UP000092445    UP000008792    UP000001070    UP000009192    UP000007801    UP000037069    UP000007798    UP000001819    UP000092553   

PRIDE

H9J8G8    F3YDM3    Q6AWN0   

Pfam

PF03079   ARD        + More
PF00217   ATP-gua_Ptrans
PF02807   ATP-gua_PtransN
PF02880   PGM_PMM_III
PF00408   PGM_PMM_IV
PF02878   PGM_PMM_I
PF02879   PGM_PMM_II

Interpro

IPR004313   ARD        + More
IPR014710   RmlC-like_jellyroll       
IPR027496   ARD_euk       
IPR011051   RmlC_Cupin_sf       
IPR000749   ATP-guanido_PTrfase       
IPR014746   Gln_synth/guanido_kin_cat_dom       
IPR036802   ATP-guanido_PTrfase_N_sf       
IPR022414   ATP-guanido_PTrfase_cat       
IPR022415   ATP-guanido_PTrfase_AS       
IPR022413   ATP-guanido_PTrfase_N       
IPR016066   A-D-PHexomutase_CS       
IPR005846   A-D-PHexomutase_a/b/a-III       
IPR005844   A-D-PHexomutase_a/b/a-I       
IPR036900   A-D-PHexomutase_C_sf       
IPR005845   A-D-PHexomutase_a/b/a-II       
IPR005841   Alpha-D-phosphohexomutase_SF       
IPR016055   A-D-PHexomutase_a/b/a-I/II/III       
IPR005843   A-D-PHexomutase_C       

SUPFAM

SSF51182   SSF51182        + More
SSF55931   SSF55931       
SSF48034   SSF48034       
SSF53738   SSF53738       
SSF55957   SSF55957       

Gene 3D

2.60.120.10     1.10.135.10    

ProteinModelPortal

H9J8G8   A0A2A4K8L0   A0A2H1VUW4   A0A212FID1   A0A0N0PBR1   A0A194PQ97   + More
A0A1Q3FGT6   A0A0L7QU20   A0A088AS00   E2ATE3   A0A195EKJ9   E9ILG6   A0A0N0BK05   A0A023EJ12   A0A154PF83   A0A2A3EEP1   A0A023EIH8   B0XKA4   A0A023EH95   A0A158NJM9   U5EVG3   Q0IFH8   A0A151X6J6   A0A195BFV5   B0WLS7   A0A1L8EB68   D6WC15   A0A151IG80   A0A182JI12   A0A232F8M7   A0A084VCA3   F4W3U3   A0A182LJ71   Q7Q6X6   A0A182XMZ5   A0A182YRS4   A0A182UBX4   A0A2M3Z803   A0A182LW86   A0A1I8P6L2   A0A182UQ92   A0A182HWV2   A0A1I8MGE8   A0A2M4C0U9   A0A182FDM5   A0A182RRV6   A0A2M4AK19   A0A182NGT7   A0A1L8DC72   A0A2M3Z884   A0A2M4C0W1   A0A182KCJ4   A0A182WB23   W5JVE7   A0A182PHJ8   B4PEG5   A0A182QUH6   A0A069DNU6   V5GRX8   F3YDM3   Q6AWN0   A0A1W4UUT0   A0A195FMX8   B3NCM6   T1DP42   S4NX86   A0A034WT85   A0A0C9PSL3   B4QP87   A0A067QFY6   A0A1W4XFG1   R4G8L3   A0A0P4VVG9   R4G4H3   A0A1J1IH66   K7IUI2   R4UW56   A0A026VTR3   A0A182HA72   E0W481   A0A0K8TQU2   A0A224XZD1   A0A1A9ZF26   A0A023F9B7   A0A1I8NBE5   B4LB52   A0A336MGM1   B4J0Y0   A0A1Y1NHT5   A0A336K6P2   B4L9H0   A0A0A1WHA6   A0A2R7WU76   B3M5D1   A0A0L0C741   B4MLV1   Q2LZI9   A0A2M4AL13   A0A0A9WLE1   A0A0M4EZ24  

PDB

1VR3     E-value=5.70195e-54,     Score=529

KEGG

101740178    K08967 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase [EC:1.13.11.53 1.13.11.54] | (RefSeq) 1,

PATHWAY

00270    Cysteine and methionine metabolism - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)

GO

GO:0010309   GO:0005737   GO:0005506   GO:0005634   GO:0019509   GO:0016021   GO:0006555   GO:2000243   GO:0005886   GO:0016491   GO:0009086   GO:0005524   GO:0016301   GO:0016868   GO:0005975   GO:0000287   GO:0016020   GO:0008237   GO:0051103   GO:0003677   GO:0006281   GO:0048384   GO:0055114  

PANTHER

PTHR23418   PTHR11547  

Subcellular location

Cytoplasm  
Nucleus  

Length:

184

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.02379

Exp number, first 60 AAs:

0

Total prob of N-in:

0.15419

outside

1  -  184

 

 

Pi

29.95674

Theta

29.782892

Tajima's D

0.025976

CLR

0.342469

CSRT

0.380480975951202

Interpretation

Uncertain