Gene

KWMTBOMO03087

Pre Gene Modal

BGIBMGA005815

Annotation

PREDICTED:_peptidyl-alpha-hydroxyglycine_alpha-amidating_lyase_1_[Bombyx_mori]

Location

Bomo_Chr5(-):17716205-17732293      View in EpiBrowser

Full name

Peptidyl-alpha-hydroxyglycine alpha-amidating lyase 1      

Alternative Name

Peptidylamidoglycolate lyase 1
dPAL1

Location in the cell

PlasmaMembrane   Reliability : 1.881

CDS

ATGAATAATTTATTATGCTTATTCAAAGTGATCGTTATGATACTGTGCTTAAGAGAATCAATTTACGTAAATGGATTTGGAGAGCGGGCCTATTTTTCAGACCGAGAAGACAATAATCCTGCATATTATATATCACCTGCATTGCAAAAACTAGAGCATGCACCAGAATTTGTGAAAGACTGGCCGAATATATCAATAACACTAGGCCAAGTTTCCGGAGTAACATTAGACAATGCAGGCAGGGTGCTAGTCTTCCACCGAGGTGAACATACTTGGGATGAGCACACATTTACAAATGGAAATATATACTTGGGAATTGGAAAACCAGCCATACAGCACAAAACTATTTTAATCTTCAATGAAACAGGAGAACTTCTTGATATGTGGGGTGATGATTTTTTTTATATGCCCCATGGCATAACGGTGGACTCTGAAGGTAATGTTTGGACCACTGATGTGGCACTACATCAAGTGTTCAAATTTAATGCTACCAACCGACGAGTGCCTGCTATGGTGCTGGGAGAAAAGTTTGTACCGGGAACAGATGACCATCATTTTTGTAAGCCAAGCGCGGTGGCCGTGTTGTCGACCGGTGACTTTTTCATAGCAGACGGCTACTGCAACCACAGGATCCTGAAATATAACCCTAAAGGGCAAATCATACTGCAGTGGGGTACTCATAACTCACACTCGCCGTTCTCTCTGAACGTGCCCCATGCGCTGGCGCTTGCTGAGAACTCGGGGGTGGTGCTGGTGGCGGACCGGGAGAGGGGCAGGGTCGCCAGCTTCAGGCACGACAACGGCTCCTACGTCACCTCCTACAGCAGCTGGCTCATGGGACCCAGGATCTTCAGTGTGGCCTATTCTCCGGTTCACGGAGGTCGTCTGTACGTGGTGAACGGGCCGAACGGCGTGGTCCCGGTGCGCGGCTACGTGCTGGAGCTGGCGTCCGGGCGGCTCGTGGGCACGTTCGACGCGCCCGCCGGCCTCGCCAGCCCGCACGACGTGGCGGCCGCGACCGACGGCTCCGCGGTCTACGTCGCGCAGCTCGCCCCGCACGCCGTCTACAAGTTCGTCGACGAGCGCCTGCGCGACGAGGCGCCGCCCGCACCCCCCGCACCCCCCGCGCCGCCCGCCGCGCCCCCCGCCACGCCCGCCACTCTCGGTGAGTAG

Protein

MNNLLCLFKVIVMILCLRESIYVNGFGERAYFSDREDNNPAYYISPALQKLEHAPEFVKDWPNISITLGQVSGVTLDNAGRVLVFHRGEHTWDEHTFTNGNIYLGIGKPAIQHKTILIFNETGELLDMWGDDFFYMPHGITVDSEGNVWTTDVALHQVFKFNATNRRVPAMVLGEKFVPGTDDHHFCKPSAVAVLSTGDFFIADGYCNHRILKYNPKGQIILQWGTHNSHSPFSLNVPHALALAENSGVVLVADRERGRVASFRHDNGSYVTSYSSWLMGPRIFSVAYSPVHGGRLYVVNGPNGVVPVRGYVLELASGRLVGTFDAPAGLASPHDVAAATDGSAVYVAQLAPHAVYKFVDERLRDEAPPAPPAPPAPPAAPPATPATLGE

Description

Probable lyase that catalyzes an essential reaction in C-terminal alpha-amidation of peptides. Mediates the dismutation of the unstable peptidyl(2-hydroxyglycine) intermediate to glyoxylate and the corresponding desglycine peptide amide. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity.

Catalytic Activity

a [peptide]-C-terminal (2S)-2-hydroxyglycine = a [peptide]-C-terminal amide + glyoxylate

Cofactor

Zn(2+)

Biophysicochemical Properties

45 uM for peptidyl-alpha-hydroxyglycine

Similarity

Belongs to the peptidyl-alpha-hydroxyglycine alpha-amidating lyase family.

Keywords

Cell membrane   Complete proteome   Disulfide bond   Glycoprotein   Lyase   Membrane   Metal-binding   Reference proteome   Repeat   Signal   Transmembrane   Transmembrane helix   Zinc  

Feature

chain  Peptidyl-alpha-hydroxyglycine alpha-amidating lyase 1

Uniprot

H9J8H3   A0A2A4J8Y4   A0A2H1WA59   A0A0N0PBR4   A0A212EKT6   A0A194PQV8   + More
A0A2J7QPM8   A0A2J7QPM1   A0A1D2NEY4   A0A067RHL7   A0A1Q3FGN2   A0A1Q3FGH4   A0A182KRQ4   A0A1A9ZCM1   B0VZE5   A0A182WIP2   A0A1A9X3N3   T1P8C3   A0A182M7E9   T1PM65   A0A1B0BH65   A0A182TDV8   A0A1A9YAZ3   A0A182RHT7   A0A1I8M5C6   A0A182P3M3   A0A182UW58   A0A1B0GDJ1   A0A084VWY7   Q7QJP6   A0A182IGW0   A0A1A9VP13   A0A182XZ69   A0A0L0BPQ8   A0A226F6T8   A0A1B6LWW9   A0A1B6G878   A0A182X3E7   C3YQ66   A0A1S3I9G3   A0A1S3IAE2   A0A1B6IDE5   A0A182GNQ6   A0A023ESW6   B4MRI5   A0A1I8PLY6   A0A182H9U8   B4J9H1   A0A182QCA2   A0A034W4J0   A0A182NHJ0   A0A0K8TYP3   A0A1W4UZC7   A0A0K8V2X3   Q17KT9   A0A168L575   A0A0A1XSQ6   A0A0K8WH17   W5JRY6   A0A0K8VGX3   A0A1W4UZ23   A0A2M3ZE61   A0A1W4V0N2   A0A2M3Z6W7   A0A0A1WQ78   A0A1Q1NAE3   A0A2M4BLU6   K7IX33   W8BY31   A0A0N8P0F3   B3N6T2   A0A0Q5WLM8   A0A0K2VHL2   A0A0B4LF14   B3MF37   B4LJX2   A0A0P8ZRB0   A0A0Q9WCX2   Q8I1H1   A0A2M4ANV6   B6IDN2   Q9V5E1   A0A224XRL7   A0A0M4EWG8   A0A182FI38   A0A0Q9X7Q6   G8EWC9   B4HMA5   A0A0R1DU55   A0A0J9RA86   B4KNT5   A0A0R1DMP8   B4NX21   T1KEL8   A0A154NX67   A0A0J9TXQ2   A0A3L8E0K9   A0A0K2VHQ9  

EC Number

4.3.2.5

Pubmed

19121390    26354079    22118469    27289101    24845553    20966253    + More
25315136    24438588    12364791    14747013    17210077    25244985    26108605    18563158    26483478    24945155    17994087    25348373    17510324    25830018    20920257    23761445    20075255    24495485    10731132    12537568    12537572    12537573    12537574    16110336    17569856    17569867    26109357    26109356    12537569    15198673    17550304    22936249    30249741   

EMBL

BABH01027407    BABH01027408    BABH01027409    BABH01027410    BABH01027411    BABH01027412    + More
NWSH01002324    PCG68525.1    ODYU01007288    SOQ49933.1    KQ460847    KPJ11991.1    AGBW02014213    OWR42099.1    KQ459597    KPI95129.1    NEVH01012088    PNF30531.1    PNF30532.1    LJIJ01000063    ODN03814.1    KK852678    KDR18657.1    GFDL01008332    JAV26713.1    GFDL01008400    JAV26645.1    DS231814    EDS31682.1    KA644956    AFP59585.1    AXCM01005430    KA649162    KA649170    AFP63799.1    JXJN01014195    CCAG010011915    ATLV01017808    KE525192    KFB42481.1    AAAB01008807    EAA04373.4    APCN01000215    JRES01001567    KNC21953.1    LNIX01000001    OXA65207.1    GEBQ01031647    GEBQ01023221    GEBQ01018526    GEBQ01011799    GEBQ01008105    GEBQ01003199    JAT08330.1    JAT16756.1    JAT21451.1    JAT28178.1    JAT31872.1    JAT36778.1    GECZ01026414    GECZ01011107    JAS43355.1    JAS58662.1    GG666540    EEN57570.1    GECU01022767    GECU01020157    GECU01017105    GECU01002011    JAS84939.1    JAS87549.1    JAS90601.1    JAT05696.1    JXUM01014692    KQ560410    KXJ82556.1    GAPW01001203    JAC12395.1    CH963850    EDW74724.2    JXUM01004851    JXUM01004852    KQ560182    KXJ83967.1    CH916367    EDW01452.1    AXCN02000593    GAKP01008431    JAC50521.1    GDHF01032923    JAI19391.1    GDHF01019067    JAI33247.1    CH477221    EAT47318.1    KU881664    ANC48002.1    GBXI01000372    JAD13920.1    GDHF01001970    JAI50344.1    ADMH02000632    ETN65499.1    GDHF01014150    JAI38164.1    GGFM01006038    MBW26789.1    GGFM01003500    MBW24251.1    GBXI01013719    JAD00573.1    KY011960    AQM52455.1    GGFJ01004884    MBW54025.1    AAZX01006819    GAMC01012391    JAB94164.1    CH902619    KPU76964.1    CH954177    EDV58181.2    KQS70322.1    HACA01032602    CDW49963.1    AE013599    AHN56057.1    EDV37665.2    CH940648    EDW61626.1    KPU76963.1    KRF80119.1    AY190939    AAO00999.1    GGFK01009140    MBW42461.1    BT050472    ACJ13179.1    ACL83084.2    AY051842    GFTR01005775    JAW10651.1    CP012524    ALC42145.1    CH933808    KRG04384.1    JF967750    AEI84584.1    CH480816    EDW47182.1    CM000157    KRJ98547.1    CM002911    KMY92624.1    EDW08980.1    KRJ98548.1    EDW89582.1    CAEY01000026    KQ434777    KZC04171.1    KMY92625.1    QOIP01000002    RLU25558.1    HACA01032603    CDW49964.1   

Proteomes

UP000005204    UP000218220    UP000053240    UP000007151    UP000053268    UP000235965    + More
UP000094527    UP000027135    UP000075882    UP000092445    UP000002320    UP000075920    UP000091820    UP000075883    UP000092460    UP000075902    UP000092443    UP000075900    UP000095301    UP000075885    UP000075903    UP000092444    UP000030765    UP000007062    UP000075840    UP000078200    UP000076408    UP000037069    UP000198287    UP000076407    UP000001554    UP000085678    UP000069940    UP000249989    UP000007798    UP000095300    UP000001070    UP000075886    UP000075884    UP000192221    UP000008820    UP000000673    UP000002358    UP000007801    UP000008711    UP000000803    UP000008792    UP000092553    UP000069272    UP000009192    UP000001292    UP000002282    UP000015104    UP000076502    UP000279307   

PRIDE

H9J8H3    B6IDN2    Q9V5E1   

Pfam

PF01436   NHL        + More
PF03712   Cu2_monoox_C
PF01082   Cu2_monooxygen

Interpro

IPR013017   NHL_repeat_subgr        + More
IPR011042   6-blade_b-propeller_TolB-like       
IPR000720   PHM/PAL       
IPR001258   NHL_repeat       
IPR001878   Znf_CCHC       
IPR036875   Znf_CCHC_sf       
IPR014783   Cu2_ascorb_mOase_CS-2       
IPR008977   PHM/PNGase_F_dom_sf       
IPR024548   Cu2_monoox_C       
IPR020611   Cu2_ascorb_mOase_CS-1       
IPR000323   Cu2_ascorb_mOase_N       
IPR014784   Cu2_ascorb_mOase-like_C       
IPR036939   Cu2_ascorb_mOase_N_sf       

SUPFAM

SSF57756   SSF57756        + More
SSF49742   SSF49742       

Gene 3D

2.120.10.30     2.60.120.230     2.60.120.310    

ProteinModelPortal

H9J8H3   A0A2A4J8Y4   A0A2H1WA59   A0A0N0PBR4   A0A212EKT6   A0A194PQV8   + More
A0A2J7QPM8   A0A2J7QPM1   A0A1D2NEY4   A0A067RHL7   A0A1Q3FGN2   A0A1Q3FGH4   A0A182KRQ4   A0A1A9ZCM1   B0VZE5   A0A182WIP2   A0A1A9X3N3   T1P8C3   A0A182M7E9   T1PM65   A0A1B0BH65   A0A182TDV8   A0A1A9YAZ3   A0A182RHT7   A0A1I8M5C6   A0A182P3M3   A0A182UW58   A0A1B0GDJ1   A0A084VWY7   Q7QJP6   A0A182IGW0   A0A1A9VP13   A0A182XZ69   A0A0L0BPQ8   A0A226F6T8   A0A1B6LWW9   A0A1B6G878   A0A182X3E7   C3YQ66   A0A1S3I9G3   A0A1S3IAE2   A0A1B6IDE5   A0A182GNQ6   A0A023ESW6   B4MRI5   A0A1I8PLY6   A0A182H9U8   B4J9H1   A0A182QCA2   A0A034W4J0   A0A182NHJ0   A0A0K8TYP3   A0A1W4UZC7   A0A0K8V2X3   Q17KT9   A0A168L575   A0A0A1XSQ6   A0A0K8WH17   W5JRY6   A0A0K8VGX3   A0A1W4UZ23   A0A2M3ZE61   A0A1W4V0N2   A0A2M3Z6W7   A0A0A1WQ78   A0A1Q1NAE3   A0A2M4BLU6   K7IX33   W8BY31   A0A0N8P0F3   B3N6T2   A0A0Q5WLM8   A0A0K2VHL2   A0A0B4LF14   B3MF37   B4LJX2   A0A0P8ZRB0   A0A0Q9WCX2   Q8I1H1   A0A2M4ANV6   B6IDN2   Q9V5E1   A0A224XRL7   A0A0M4EWG8   A0A182FI38   A0A0Q9X7Q6   G8EWC9   B4HMA5   A0A0R1DU55   A0A0J9RA86   B4KNT5   A0A0R1DMP8   B4NX21   T1KEL8   A0A154NX67   A0A0J9TXQ2   A0A3L8E0K9   A0A0K2VHQ9  

PDB

5WM0     E-value=1.35006e-61,     Score=599

KEGG

105841291    K18200 peptidylamidoglycolate lyase [EC:4.3.2.5] | (RefSeq) peptidyl-alpha-hydroxyglycine alpha-amid

GO

GO:0008270   GO:0003824   GO:0003676   GO:0006518   GO:0016021   GO:0016020   GO:0016829   GO:0016715   GO:0005507   GO:0004497   GO:0004598   GO:0001519   GO:0005576   GO:0004504   GO:0043025   GO:0044719   GO:0005886   GO:0007619   GO:0046872   GO:0005515   GO:0008237   GO:0051103   GO:0003677   GO:0006281   GO:0048384  

Subcellular location

Cell membrane   Confined to cell bodies.   With evidence from 3 publications.

SignalP

Position:   1 - 25,         Likelihood:  0.799207

 

 

Length:

390

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.999229999999999

Exp number, first 60 AAs:

0.99123

Total prob of N-in:

0.05222

outside

1  -  390

 

 

Pi

220.486825

Theta

165.466216

Tajima's D

1.324054

CLR

0.929944

CSRT

0.741862906854657

Interpretation

Uncertain