Gene

KWMTBOMO02956  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA003567

Annotation

alkaliphilic_serine_protease_[Bombyx_mori]

Location

Bomo_Chr5(+):14398565-14401133      View in EpiBrowser

Full name

Trypsin, alkaline A       + More
Trypsin, alkaline C      
Trypsin, alkaline B      
Trypsin CFT-1      

Location in the cell

Extracellular   Reliability : 4.75

CDS

ATGCGGTCTACCATTATTTTGTTGGCCCTTGGGCTTGCTGCTGTGGCGGCTGTTCCTACCAACCCACAAAGGATTATTGGTGGCTCCACGACCAACATTAACCAGTATCCCGGTATAGCTGCTTTGTTGTATACATGGAATTGGAATCAGTGGTGGCAGTCTTGTGGTGGAAACATCCTTAACCAGAGATCTATCCTTAGTGCCGCTCACTGTCCATACGGTGACGCTACAGGCCGTTGGCGTATTCGTGTTGGTTCCACCTTTGCCAACAGTGGGGGTGTTGTGCATAACGTGAACAGAATTATCATTCATCCCAACTATAACAGACGTACTGCTGACAGTGACCTCTGCATCTTACGCAGTAATTCCAACATAGCTTATAACAACAACGTTCGTCCTATTAATATTGCTGGTGCCAATTACAATCTTGGTGATAACCAAGTTGTTTGGGCTGCTGGTTGGGGTGCTACATCGCTCGGAGGGTCTAATTCGGAGCAACTCCGTCACGTCCAGGTCTGGACCATCAATCAGAATGCCTGCGTCCAACGTTACAGACCCATTAACCGTGCTATCACCGCTAACATGTTGTGCTCTGGTGTTTTGGACGTCGGTGGTCGCGACCAGTGCCAGGGTGACTCCGGTGGTCCTCTCCTCCACAACCGCGTCCTCGTTGGTGTCTGCTCTTGGGGACAGTACTGCGCTGACCGTCGCTTCCCTGGTGTTAACGTTCGCGTTTCCCGTTTCACCTCTTGGATCCAATCCAATTCGTAA

Protein

MRSTIILLALGLAAVAAVPTNPQRIIGGSTTNINQYPGIAALLYTWNWNQWWQSCGGNILNQRSILSAAHCPYGDATGRWRIRVGSTFANSGGVVHNVNRIIIHPNYNRRTADSDLCILRSNSNIAYNNNVRPINIAGANYNLGDNQVVWAAGWGATSLGGSNSEQLRHVQVWTINQNACVQRYRPINRAITANMLCSGVLDVGGRDQCQGDSGGPLLHNRVLVGVCSWGQYCADRRFPGVNVRVSRFTSWIQSNS

Catalytic Activity

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Similarity

Belongs to the peptidase S1 family.

Keywords

Disulfide bond   Hydrolase   Protease   Secreted   Serine protease   Signal   Zymogen   Direct protein sequencing  

Feature

propeptide  Activation peptide
chain  Trypsin, alkaline A

Uniprot

H9J230   J7JYV8   H9J229   Q9TXE6   P35045   P35047   + More
A0A1L6UW50   A0A194PNR3   P35046   Q27540   P35042   A0A2U8NFD7   A0A194PNR6   A0A194PP33   A0A0N1I947   Q961Y0   B4X995   A0A2A4K5G7   B4X996   A0A2A4K6E9   B4X9A1   O18447   A0A2A4K648   C9W8F7   V9M0R7   A0A2A4K6E3   J7IJ83   Q4L1K4   O76954   A0A194PQQ5   A0A2H1VJ41   A0A2A4K747   A0A0N1PHJ0   S5PVP1   A0A2W1BA14   A0A2A4K5F8   A0A2A4K5I4   A0A2W1BQW9   A0A194PQ27   A0A2W1B606   H9J231   A0A2H1VPG9   H9J243   A0A194PVB6   Q4L1K6   J7IF09   A0A1B0RHN7   O18434   B4X9A0   Q4L1M0   A0A1C9M3A1   O18436   A0A2W1BL45   A0A2W1BKF9   A0A2W1BF78   A0A2W1BKV8   A0A2A4K769   B3F884   J7IFG7   I7E449   A0A2W1BP46   A0A2A4K6U2   A0A0K8SII3   A0A2W1BGQ7   A0A2W1B1G1   E7D004   A0A2A4K6W3   A0A2A4K6F3   C4PFX2   A0A2W1BP39   W5X3N9   I7DEI4   A0A194PQQ0   O62598   V9LL27   Q56IB6   A0A2H1W4N7   I7DHL6   A0A2W1BSA6   D7S002   A0A2A4K636   Q56IB8   Q4L1K8   Q56IB4   O18435   Q6R561   A0A2A4K756   D7S000   A0A2H1WNB6   A0A2W1BE44   Q56IB5   Q4L1L9   Q56IB7   Q4L1L8   A0A2W1BB90   D7S001   A0A2A4K5I0   C9W8J5   J7IN23   A0A2H1W9X8  

EC Number

3.4.21.4

Pubmed

19121390    8462672    8205142    24801594    26354079    7550246    + More
8294037    8353523    12110299    9404008    28756777    20824821    23247047    15979001    23606528    19146482    20545748    22824002    10672067    16469064    15944081   

EMBL

BABH01007517    JX312360    AFQ59994.1    BABH01007520    L16805    L16807    + More
KY379952    APS85762.1    KQ459597    KPI95066.1    L16806    U12917    AAA84423.1    L04749    MF407317    AWL83213.1    KPI95071.1    KPI95067.1    KQ460226    KPJ16474.1    AY040819    AAK81696.1    EF531631    EF531633    ABR88242.1    ABR88244.1    NWSH01000106    PCG79485.1    EF531632    ABR88243.1    PCG79488.1    EF531637    ABR88248.1    HM209426    Y12283    KZ150104    ADI32887.1    CAA72962.1    PZC73444.1    PCG79486.1    FJ205402    ACR15970.1    JX866720    AGB93875.1    PCG79474.1    JX195651    AFQ37934.1    AY587161    AAT95353.1    AJ007706    CAA07611.1    KPI95074.1    ODYU01002655    SOQ40442.1    PCG79472.1    KPJ16473.1    KC175562    AGR92345.1    KZ150747    PZC70337.1    PCG79475.1    PCG79487.1    KZ149992    PZC75527.1    KPI95068.1    PZC70335.1    BABH01007514    BABH01007515    ODYU01003655    SOQ42698.1    BABH01007473    KPI95070.1    AY587159    AAT95351.1    JX195649    AFQ37932.1    KM360188    AKH49604.1    Y12269    CAA72948.1    PZC73449.1    EF531636    ABR88247.1    PCG79483.1    AY587145    AAT95360.1    KU302250    AOQ30448.1    EF104916    Y12271    ABP96918.1    CAA72950.1    PZC73450.1    PZC75529.1    PZC73448.1    PZC75528.1    PCG79490.1    EF635223    ABU96714.1    JX195652    AFQ37935.1    JX046916    AFO83992.1    PZC73453.1    PCG79473.1    GBRD01013231    JAG52595.1    PZC73441.1    PZC70338.1    HM990174    ADT80822.1    PCG79493.1    PCG79484.1    FJ940726    ODYU01006287    ACR25157.1    SOQ47996.1    PZC73443.1    KF779933    AHI07442.1    JN793539    AFO68319.1    KPI95069.1    AF064525    AF064526    AAC36247.1    AAC36248.1    JQ340915    AFP74111.1    AY953061    AAX62034.1    ODYU01007611    SOQ47997.1    SOQ50538.1    JN793542    AFO68322.1    PZC75526.1    HM209425    ADI32886.1    PCG79476.1    AY953059    AAX62032.1    AY587157    AAT95349.1    AY953063    AAX62036.1    Y12270    CAA72949.1    AY513649    AAR98918.1    PCG79482.1    HM209423    ADI32884.1    ODYU01009856    SOQ54559.1    PZC73442.1    AY953062    AAX62035.1    AY587146    AAT95361.1    AY953060    AAX62033.1    AY587147    AAT95362.1    PZC70336.1    HM209424    ADI32885.1    PCG79481.1    FJ205445    ACR16008.2    JX195648    AFQ37931.1    ODYU01007266    SOQ49895.1   

Proteomes

UP000005204    UP000053268    UP000053240    UP000218220   

PRIDE

H9J230    J7JYV8    H9J229    Q9TXE6    P35045    P35047    + More
P35042    H9J231    H9J243   

Pfam

PF00089   Trypsin

Interpro

IPR001254   Trypsin_dom        + More
IPR033116   TRYPSIN_SER       
IPR009003   Peptidase_S1_PA       
IPR001314   Peptidase_S1A       
IPR018114   TRYPSIN_HIS       

SUPFAM

SSF50494   SSF50494       

CDD

cd00190  Tryp_SPc

ProteinModelPortal

H9J230   J7JYV8   H9J229   Q9TXE6   P35045   P35047   + More
A0A1L6UW50   A0A194PNR3   P35046   Q27540   P35042   A0A2U8NFD7   A0A194PNR6   A0A194PP33   A0A0N1I947   Q961Y0   B4X995   A0A2A4K5G7   B4X996   A0A2A4K6E9   B4X9A1   O18447   A0A2A4K648   C9W8F7   V9M0R7   A0A2A4K6E3   J7IJ83   Q4L1K4   O76954   A0A194PQQ5   A0A2H1VJ41   A0A2A4K747   A0A0N1PHJ0   S5PVP1   A0A2W1BA14   A0A2A4K5F8   A0A2A4K5I4   A0A2W1BQW9   A0A194PQ27   A0A2W1B606   H9J231   A0A2H1VPG9   H9J243   A0A194PVB6   Q4L1K6   J7IF09   A0A1B0RHN7   O18434   B4X9A0   Q4L1M0   A0A1C9M3A1   O18436   A0A2W1BL45   A0A2W1BKF9   A0A2W1BF78   A0A2W1BKV8   A0A2A4K769   B3F884   J7IFG7   I7E449   A0A2W1BP46   A0A2A4K6U2   A0A0K8SII3   A0A2W1BGQ7   A0A2W1B1G1   E7D004   A0A2A4K6W3   A0A2A4K6F3   C4PFX2   A0A2W1BP39   W5X3N9   I7DEI4   A0A194PQQ0   O62598   V9LL27   Q56IB6   A0A2H1W4N7   I7DHL6   A0A2W1BSA6   D7S002   A0A2A4K636   Q56IB8   Q4L1K8   Q56IB4   O18435   Q6R561   A0A2A4K756   D7S000   A0A2H1WNB6   A0A2W1BE44   Q56IB5   Q4L1L9   Q56IB7   Q4L1L8   A0A2W1BB90   D7S001   A0A2A4K5I0   C9W8J5   J7IN23   A0A2H1W9X8  

PDB

1EKB     E-value=2.92483e-29,     Score=318

KEGG

101738353    no KO assigned | (RefSeq) trypsin, alkaline C-like

GO

GO:0004252   GO:0005615   GO:0006508   GO:0006811   GO:0015379   GO:0016021   GO:0003909   GO:0051103   GO:0003677   GO:0006281   GO:0048384  

Subcellular location

  

SignalP

Position:   1 - 17,         Likelihood:  0.998356

 

 

Length:

256

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

3.7294

Exp number, first 60 AAs:

3.70564

Total prob of N-in:

0.17413

outside

1  -  256

 

 

Pi

208.423842

Theta

3.552175

Tajima's D

-1.004291

CLR

31.204866

CSRT

0.130393480325984

Interpretation

Uncertain