Gene

KWMTBOMO02928  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA003637

Annotation

acid_phosphatase_isoenzyme_[Bombyx_mori]

Location

Bomo_Chr5(-):14071084-14072715      View in EpiBrowser

Full name

Low molecular weight phosphotyrosine protein phosphatase       + More
DNA topoisomerase I      

Alternative Name

Low molecular weight cytosolic acid phosphatase
Adipocyte acid phosphatase
Red cell acid phosphatase 1
DNA topoisomerase 1

Location in the cell

Cytoplasmic   Reliability : 2.125

CDS

ATGGTCGACCACAAGAAAAAGGCATTATTTATTTGTCTCGGTAATATATGTCGGTCACCAATTGCTGAGGCTGTTTTCCAGAAAACCGTAAATGACATGAATCTGGGTGAACATTGGGATATCGATAGTGCAGCTATCGGGGGCTGGCATGTCGGTAATCCTCCCGATTGGAGAGCACTAGATACTTTGAAGAAGCATAACGTTCCATACAACAACCATGCAAGACAAATAACTTCTGAAGACTTCGATTATTATGATTATATCTTTGGTATGGATGAATCCAATATGAAAGATTTGAACAAAAAAGCTCCTAAAGGGAGTAAAGCGAAACTACTGTTATTTGGAGATTTTGACCCTCAAGGCGATCGAATCATAAGAGATCCATACTATGATAGTGATTCCAAAGGCTTTGAAAAATGTTACGAGCAGTCGGTTCGATGCTCTAAAGGATTTTTGGAGCATATTGCAAATGAAAAATGA

Protein

MVDHKKKALFICLGNICRSPIAEAVFQKTVNDMNLGEHWDIDSAAIGGWHVGNPPDWRALDTLKKHNVPYNNHARQITSEDFDYYDYIFGMDESNMKDLNKKAPKGSKAKLLLFGDFDPQGDRIIRDPYYDSDSKGFEKCYEQSVRCSKGFLEHIANEK

Description

Acts on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates.
Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at the specific target site 5'-[CT]CCTTp site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Catalytic Activity

H2O + O-phospho-L-tyrosyl-[protein] = L-tyrosyl-[protein] + phosphate
a phosphate monoester + H2O = an alcohol + phosphate
ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.

Subunit

Interacts with the SH3 domain of SPTAN1. Interacts with EPHA2; dephosphorylates EPHA2. Interacts with EPHB1 (By similarity).
Isoform 1 interacts with the SH3 domain of SPTAN1. There is no interaction observed for isoforms 2 or 3. Interacts with EPHA2; dephosphorylates EPHA2. Interacts with EPHB1.

Similarity

Belongs to the low molecular weight phosphotyrosine protein phosphatase family.
Belongs to the type IB topoisomerase family.

Keywords

Acetylation   Complete proteome   Cytoplasm   Hydrolase   Phosphoprotein   Protein phosphatase   Reference proteome   3D-structure   Alternative splicing   Direct protein sequencing   Polymorphism  

Feature

chain  Low molecular weight phosphotyrosine protein phosphatase
splice variant  In isoform 2.
sequence variant  In dbSNP:rs11691572.

Uniprot

Q2F633   A0A2H1VVE0   A0A0N1IIJ7   A0A2W1BQY5   I4DK46   A0A194PP10   + More
S4PGI5   H9J2A0   A0A212FIB1   A0A0L7KQI4   A0A2A4K498   A0A182H346   A0A023EHL7   Q17G80   A0A1S4F474   U4UD79   A0A336KGL4   N6TBL0   A0A182K6L2   A0A212FIB5   A0A2M4C276   A0A2M4AR23   A0A2M3ZA51   A0A182R3P4   A0A2M4AQZ5   W5JL91   A0A182IH89   A0A182XFH6   Q7QH56   A0A182FQ78   A0A2M4C1S3   A0A1L8DM48   A0A1B6FZE5   A0A1B6LF00   A0A182MAU4   A0A182QP25   A0A1L8DLF9   A0A084VER1   V5GEX5   B0WL23   A0A182NG68   U5EK34   A0A182YKJ5   T1P8A3   A0A1Q3FGV1   A0A067QXJ3   A0A1I8NHA7   A0A2J7RLY4   A0A1J1HL98   A0A1L8EGU7   A0A336MJZ6   A0A1L8EH79   A0A0N8D4U7   A0A1L8EDE0   A0A1Y1LRE4   A0A0P5EBR6   A0A139W9P1   A0A0P6C4W7   A0A0P4WGK3   A0A182IKK5   T1IAS2   A0A2P8YH83   A0A1I8NVJ1   A0A170ZNH0   A0A0P4ZMK8   A0A0K8RHA6   A0A131Y1Z6   A0A182SP20   A0A131YPR7   T1DID7   A0A0P4VG93   L7M3X5   A0A0P5LWS8   A0A384BTH3   A0A0V0G4S1   A0A224Z3I6   A0A0A9VZZ0   A0A2K6FQ21   A0A2J8SHE4   Q5REM7   G3S306   K7AXM4   P24666   I6L591   A0A0K8VAT2   A0A146KP83   A0A2I3GWY7   Q59EH3   W8AND9   A0A182VX44   A0A2K5C331   A0A2R8M4M0   U3CJJ6   A0A1D2N6P4   A0A2Y9HMV0   A0A034WVJ0   A0A146KT00   A0A2K6V7Q6   A0A2K6C2L3   A0A2K5YI40  

EC Number

3.1.3.48
5.6.2.2

Pubmed

26354079    28756777    22651552    23622113    19121390    22118469    + More
26227816    26483478    24945155    17510324    23537049    20920257    23761445    12364791    14747013    17210077    24438588    25244985    24845553    25315136    28004739    18362917    19820115    29403074    26830274    27129103    25576852    28797301    25401762    22398555    16136131    1939112    1627603    1587862    8586411    1304913    9038134    10336608    14702039    16344560    15815621    15489334    8364553    9499402    12167657    11971983    21269460    24275569    25944712    9705307    26823975    24495485    25243066    27289101    25348373   

EMBL

DQ311239    ABD36184.1    ODYU01004659    SOQ44778.1    KQ460226    KPJ16452.1    + More
KZ149992    PZC75547.1    AK401664    BAM18286.1    KQ459597    KPI95047.1    GAIX01006200    JAA86360.1    BABH01007545    AGBW02008399    OWR53482.1    JTDY01007284    KOB65326.1    NWSH01000167    PCG78856.1    JXUM01106562    KQ565066    KXJ71387.1    GAPW01005217    JAC08381.1    CH477265    EAT45568.1    KB632003    ERL87870.1    UFQS01000396    UFQT01000396    SSX03599.1    SSX23964.1    APGK01044227    KB741021    ENN75093.1    OWR53483.1    GGFJ01010120    MBW59261.1    GGFK01009899    MBW43220.1    GGFM01004640    MBW25391.1    GGFK01009878    MBW43199.1    ADMH02001241    ETN63534.1    APCN01002607    AAAB01008817    EAA05379.5    GGFJ01010121    MBW59262.1    GFDF01006650    JAV07434.1    GECZ01025769    GECZ01014248    GECZ01006801    GECZ01000503    JAS44000.1    JAS55521.1    JAS62968.1    JAS69266.1    GEBQ01017710    JAT22267.1    AXCM01000651    AXCN02001190    GFDF01006879    JAV07205.1    ATLV01012288    KE524778    KFB36455.1    GALX01005942    JAB62524.1    DS231979    EDS30186.1    GANO01001966    JAB57905.1    KA644921    AFP59550.1    GFDL01008293    JAV26752.1    KK852855    KDR14944.1    NEVH01002587    PNF41842.1    CVRI01000010    CRK88832.1    GFDG01000839    JAV17960.1    UFQS01001481    UFQT01001481    SSX11164.1    SSX30734.1    GFDG01000840    JAV17959.1    GDIQ01210773    GDIP01068671    LRGB01002580    JAK40952.1    JAM35044.1    KZS07003.1    GFDG01002127    JAV16672.1    GEZM01052502    JAV74475.1    GDIP01145213    JAJ78189.1    KQ972166    KYB24625.1    GDIP01009147    JAM94568.1    GDRN01005948    JAI67957.1    ACPB03000063    PYGN01000596    PSN43619.1    GEMB01002038    JAS01141.1    GDIP01223703    JAI99698.1    GADI01003298    JAA70510.1    GEFM01003406    JAP72390.1    GEDV01007303    JAP81254.1    GAMD01001936    JAA99654.1    GDKW01003400    JAI53195.1    GACK01007181    JAA57853.1    GDIQ01166991    JAK84734.1    GECL01003081    JAP03043.1    GFPF01009486    MAA20632.1    GBHO01042481    GBHO01042480    JAG01123.1    JAG01124.1    NDHI03003569    PNJ20189.1    CR857496    CABD030014614    AACZ04059813    GABC01000843    GABF01009512    GABD01001161    GABE01010299    NBAG03000514    JAA10495.1    JAA12633.1    JAA31939.1    JAA34440.1    PNI18235.1    M83653    M83654    U25849    U25847    U25848    S62884    S62885    M87545    BT007136    AK289934    AK291861    BP363227    AC079779    CH471053    BC007422    BC106011    L06508    ABGA01184549    ABGA01184550    ABGA01333779    ABGA01333780    GDHF01016331    GDHF01011354    JAI35983.1    JAI40960.1    GDHC01020760    JAP97868.1    ADFV01109824    AB209838    BAD93075.1    GAMC01016251    GAMC01016247    GAMC01016245    GAMC01016241    JAB90310.1    GAMT01000205    GAMS01002550    GAMR01008094    GAMQ01006427    GAMP01000727    JAB11656.1    JAB20586.1    JAB25838.1    JAB35424.1    JAB52028.1    LJIJ01000180    ODN00938.1    GAKP01000303    JAC58649.1    GDHC01020579    JAP98049.1   

Proteomes

UP000053240    UP000053268    UP000005204    UP000007151    UP000037510    UP000218220    + More
UP000069940    UP000249989    UP000008820    UP000030742    UP000019118    UP000075881    UP000075900    UP000000673    UP000075840    UP000076407    UP000007062    UP000069272    UP000075883    UP000075886    UP000030765    UP000002320    UP000075884    UP000076408    UP000027135    UP000095301    UP000235965    UP000183832    UP000076858    UP000007266    UP000075880    UP000015103    UP000245037    UP000095300    UP000075901    UP000261680    UP000233160    UP000001595    UP000001519    UP000002277    UP000005640    UP000001073    UP000075920    UP000233020    UP000008225    UP000094527    UP000248481    UP000233220    UP000233120    UP000233140   

PRIDE

Q2F633    H9J2A0    Q5REM7    P24666    Q59EH3   

Pfam

PF01451   LMWPc        + More
PF02919   Topoisom_I_N
PF14370   Topo_C_assoc
PF01028   Topoisom_I

Interpro

IPR036196   Ptyr_pPase_sf        + More
IPR017867   Tyr_phospatase_low_mol_wt       
IPR002115   Tyr_Pase_low_mol_wt_mml       
IPR023485   Ptyr_pPase       
IPR018521   TopoI_AS       
IPR013499   TopoI_euk       
IPR001631   TopoI       
IPR008336   TopoI_DNA-bd_euk       
IPR014711   TopoI_cat_a-hlx-sub_euk       
IPR011010   DNA_brk_join_enz       
IPR014727   TopoI_cat_a/b-sub_euk       
IPR013030   DNA_topo_DNA_db_N_dom2       
IPR013500   TopoI_cat_euk       
IPR036202   TopoI_DNA-bd_euk_N_sf       
IPR013034   DNA_topo_DNA_db_N_dom1       
IPR025834   TopoI_C_dom       

SUPFAM

SSF52788   SSF52788        + More
SSF56741   SSF56741       
SSF56349   SSF56349       

Gene 3D

1.10.10.41     1.10.132.10     2.170.11.10     3.90.15.10    

CDD

cd00659  Topo_IB_C

ProteinModelPortal

Q2F633   A0A2H1VVE0   A0A0N1IIJ7   A0A2W1BQY5   I4DK46   A0A194PP10   + More
S4PGI5   H9J2A0   A0A212FIB1   A0A0L7KQI4   A0A2A4K498   A0A182H346   A0A023EHL7   Q17G80   A0A1S4F474   U4UD79   A0A336KGL4   N6TBL0   A0A182K6L2   A0A212FIB5   A0A2M4C276   A0A2M4AR23   A0A2M3ZA51   A0A182R3P4   A0A2M4AQZ5   W5JL91   A0A182IH89   A0A182XFH6   Q7QH56   A0A182FQ78   A0A2M4C1S3   A0A1L8DM48   A0A1B6FZE5   A0A1B6LF00   A0A182MAU4   A0A182QP25   A0A1L8DLF9   A0A084VER1   V5GEX5   B0WL23   A0A182NG68   U5EK34   A0A182YKJ5   T1P8A3   A0A1Q3FGV1   A0A067QXJ3   A0A1I8NHA7   A0A2J7RLY4   A0A1J1HL98   A0A1L8EGU7   A0A336MJZ6   A0A1L8EH79   A0A0N8D4U7   A0A1L8EDE0   A0A1Y1LRE4   A0A0P5EBR6   A0A139W9P1   A0A0P6C4W7   A0A0P4WGK3   A0A182IKK5   T1IAS2   A0A2P8YH83   A0A1I8NVJ1   A0A170ZNH0   A0A0P4ZMK8   A0A0K8RHA6   A0A131Y1Z6   A0A182SP20   A0A131YPR7   T1DID7   A0A0P4VG93   L7M3X5   A0A0P5LWS8   A0A384BTH3   A0A0V0G4S1   A0A224Z3I6   A0A0A9VZZ0   A0A2K6FQ21   A0A2J8SHE4   Q5REM7   G3S306   K7AXM4   P24666   I6L591   A0A0K8VAT2   A0A146KP83   A0A2I3GWY7   Q59EH3   W8AND9   A0A182VX44   A0A2K5C331   A0A2R8M4M0   U3CJJ6   A0A1D2N6P4   A0A2Y9HMV0   A0A034WVJ0   A0A146KT00   A0A2K6V7Q6   A0A2K6C2L3   A0A2K5YI40  

PDB

5KQP     E-value=8.97638e-47,     Score=466

KEGG

692857    K14394 low molecular weight phosphotyrosine protein phosphatase [EC:3.1.3.2 3.1.3.48] | (RefSeq) aci

PATHWAY

00730    Thiamine metabolism - Bombyx mori (domestic silkworm)
00740    Riboflavin metabolism - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)

GO

GO:0004726   GO:0003993   GO:0005737   GO:0004725   GO:0005829   GO:0042383   GO:0070062   GO:0009898   GO:0003677   GO:0005694   GO:0006265   GO:0003917   GO:0006470   GO:0007169   GO:0016020   GO:0004713   GO:0006281   GO:0048384  

Subcellular location

Cytoplasm  

Length:

159

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.01305

Exp number, first 60 AAs:

0.01305

Total prob of N-in:

0.21400

outside

1  -  159

 

 

Pi

186.454588

Theta

170.490092

Tajima's D

0.321183

CLR

0

CSRT

0.463076846157692

Interpretation

Uncertain