Gene

KWMTBOMO02913  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA003643

Annotation

6-pyruvoyltetrahydropterin_synthase_[Bombyx_mori]

Location

Bomo_Chr5(-):13701622-13703186      View in EpiBrowser

Full name

6-pyruvoyl tetrahydrobiopterin synthase      

Location in the cell

Nuclear   Reliability : 2.206

CDS

ATGTCTTCTTTACCTATTGTATCAATAATACGCCGTGAAACTTTCAGTTCCGCTCATAGGCTGCACAGCCCGTTTTTAAGTGACGAAGAGAACAAAAAAGTTTATGGTAAATGCAACAATCCTAATGGTCACGGTCATAATTATGTTGTTCTTGTAACAGTAAAGGGTCCTGTAGATCCTCAAACTGGTATGGTGATGAATATAACTGACCTCAAGAAATACATAAAAACAGCCATCCTGGAACCACTCGACCATAAGAATTTAGATAATGATGTTCCATACTTCAAAACTATGGCAAGTACTACAGAAAATGTCGCAATATATGTCTGGGACCAACTTCAAAGAATAATGGAAAAACCACAATTACTGCATGAGGTTAAGATCCTAGAAACTGAAAAAAATCATGTTGTGTACCGTGGTGGTAATACATACTCTAGGAAAAAGCCTGACTCCTCTTGTCACCGAAGTCATGTTTCTCATGAAGTTTCCTCAGATTCTGACTAA

Protein

MSSLPIVSIIRRETFSSAHRLHSPFLSDEENKKVYGKCNNPNGHGHNYVVLVTVKGPVDPQTGMVMNITDLKKYIKTAILEPLDHKNLDNDVPYFKTMASTTENVAIYVWDQLQRIMEKPQLLHEVKILETEKNHVVYRGGNTYSRKKPDSSCHRSHVSHEVSSDSD

Description

Involved in the biosynthesis of tetrahydrobiopterin, an essential cofactor of aromatic amino acid hydroxylases. Catalyzes the transformation of 7,8-dihydroneopterin triphosphate into 6-pyruvoyl tetrahydropterin.

Catalytic Activity

7,8-dihydroneopterin 3'-triphosphate = 6-pyruvoyl-5,6,7,8-tetrahydropterin + H(+) + triphosphate

Cofactor

Zn(2+)

Biophysicochemical Properties

8.1 uM for 7,8-dihydroneopterin triphosphate

Subunit

Homohexamer formed of two homotrimers in a head to head fashion.

Miscellaneous

The active site is at the interface between 2 subunits. The proton acceptor Cys is on one subunit, and the charge relay system is on the other subunit.

Similarity

Belongs to the PTPS family.

Keywords

3D-structure   Complete proteome   Disease mutation   Lyase   Metal-binding   Phenylketonuria   Phosphoprotein   Reference proteome   Tetrahydrobiopterin biosynthesis   Zinc  

Feature

chain  6-pyruvoyl tetrahydrobiopterin synthase
sequence variant  In HPABH4A; severe decrease in activity; diminishes phosphorylation by PKG; dbSNP:rs104894274.

Uniprot

Q2F6C9   D4AH60   A0A0N1IHA7   I4DRI8   S6B5B9   A0A0L7LSC6   + More
A0A2W1BU92   A0A2A4J807   S4PGH6   A0A212F955   A0A2H1WJT7   A0A182JLQ5   A0A182PVX2   A0A182YLP1   A0A182VTH8   A0A182NBB4   A0A182R415   A0A182KWH9   A0A182VPH3   A0A182ULF8   A0A182X683   Q7Q899   A0A182IBA4   Q17KM3   A0A182QSC5   A0A182H841   A0A084VMJ8   A0A023EI03   A0A2M4C170   A0A2M3ZIZ8   A0A1Q3FHK5   A0A2M4AMY5   W5JQR8   A0A182F3S5   A0A336LQR4   A0A067R9T2   A0A182K4W8   A0A2S2QB83   A0A0P5IV57   A0A0P4ZCR6   A0A0P4Z638   J9K2U2   A0A2S2PTB2   A0A2H8THS1   A0A232FNL9   A0A1J1I5E0   F2UQD7   A0A0K8TLH4   K7ITD3   A0A3B4FLU6   A0A0A9YN07   A0A3P8R1N2   A0A226ENK5   E9FSQ0   A0A0P7UT15   I3KU31   A0A3M0ISX8   U3JUN8   A0A1W5AV16   A0A3P9B7U4   A0A3B4A070   A0A310SFP7   A0A3B4U8Z4   A0A3B4YMX5   A0A1L8EFS2   A0A1L8EFN2   A0A1I8NER8   B4M9L7   A0A088AJ91   A0A1S3HTE3   R4WJU7   A0A2K6UF50   A0A2K5CU78   A0A2D4LL60   A0A2D4HG29   A0A2K5PQM5   A0A3P9MLS5   A0A1I8QDN9   B4KEP4   A0A182M712   A0A0M4ESB3   B4JQ90   A0A3B3CRS3   A0A3P8TH00   A0A154PNM9   A0A2D4G939   A0A2I2YV86   A0A2R9AR91   H2R4Y8   Q4SSZ9   A0A1S4E8G1   Q03393   A0A1B6K5I9   A0A1A9XL25   B4N7P2   A0A2A3EUT5   A0A1L8DZF6   A0A1B0B7P3   A0A3P9ID22   A0A096NC15  

EC Number

4.2.3.12

Pubmed

19121390    23567588    26354079    22651552    26227816    28756777    + More
23622113    22118469    25244985    20966253    12364791    14747013    17210077    17510324    26483478    24438588    24945155    20920257    23761445    24845553    28648823    26369729    20075255    25401762    26823975    21292972    25186727    25463417    25315136    17994087    23691247    17554307    29451363    22398555    22722832    16136131    15496914    1282802    8216273    7698774    8841415    15489334    18669648    20068231    23186163    24275569    10531334    9222755    8178819    7493990    8707300    9222757    9159737    9450907    10585341    10220141    10874306    11388593   

EMBL

BABH01007576    DQ311143    KR703273    AB749389    AB749393    ABD36088.1    + More
ALI58394.1    BAN66732.1    BAN66736.1    AB499124    KQ459597    BAI87830.1    KPI95031.1    KQ460226    KPJ16437.1    AK405135    BAM20528.1    AB749390    BAN66733.1    JTDY01000195    KOB78352.1    KZ149960    PZC76350.1    NWSH01002700    PCG67654.1    GAIX01003662    JAA88898.1    AGBW02009627    OWR50285.1    ODYU01009133    SOQ53340.1    AAAB01008944    EAA10120.3    APCN01000661    CH477223    EAT47203.1    EJY57388.1    AXCN02000628    JXUM01028611    JXUM01028612    JXUM01028613    KQ560826    KXJ80727.1    ATLV01014599    KE524975    KFB39192.1    GAPW01005078    JAC08520.1    GGFJ01009914    MBW59055.1    GGFM01007667    MBW28418.1    GFDL01008008    JAV27037.1    GGFK01008835    MBW42156.1    ADMH02000386    ETN66752.1    UFQT01000118    SSX20396.1    KK852607    KDR20338.1    GGMS01003214    GGMS01005587    MBY72417.1    MBY74790.1    GDIQ01207369    JAK44356.1    GDIP01218646    JAJ04756.1    GDIP01229299    JAI94102.1    ABLF02040898    GGMR01019949    MBY32568.1    GFXV01001851    MBW13656.1    NNAY01000006    OXU32109.1    CVRI01000038    CRK94134.1    GL832989    EGD79843.1    GDAI01002833    JAI14770.1    AAZX01004114    GBHO01011122    GBHO01011121    GBRD01007723    GDHC01006207    JAG32482.1    JAG32483.1    JAG58098.1    JAQ12422.1    LNIX01000003    OXA58381.1    GL732524    EFX89785.1    JARO02000739    KPP77556.1    AERX01002046    QRBI01000231    RMB91977.1    AGTO01021108    KQ768636    OAD53085.1    GFDG01001334    JAV17465.1    GFDG01001335    JAV17464.1    CH940654    EDW57893.1    AK417880    BAN21095.1    IACM01022308    LAB21744.1    IACK01028143    LAA70908.1    CH933807    EDW11923.1    AXCM01008243    CP012523    ALC40200.1    CH916372    EDV99070.1    KQ434977    KZC12830.1    IACJ01114906    LAA56292.1    CABD030081589    AJFE02014411    AJFE02014412    AJFE02014413    AACZ04006780    GABC01003131    GABF01007695    GABD01010820    GABE01005746    NBAG03000323    JAA08207.1    JAA14450.1    JAA22280.1    JAA38993.1    PNI40662.1    CAAE01014338    CAF96233.1    M97655    D17400    D25234    L76259    U63383    U63380    U63381    U63382    AB042297    EF445018    CH471065    BC009686    BC018029    GECU01000985    JAT06722.1    CH964214    EDW80381.1    KZ288185    PBC34976.1    GFDF01002236    JAV11848.1    JXJN01009696    AHZZ02007649   

Proteomes

UP000005204    UP000053268    UP000053240    UP000037510    UP000218220    UP000007151    + More
UP000075880    UP000075885    UP000076408    UP000075920    UP000075884    UP000075900    UP000075882    UP000075903    UP000075902    UP000076407    UP000007062    UP000075840    UP000008820    UP000075886    UP000069940    UP000249989    UP000030765    UP000000673    UP000069272    UP000027135    UP000075881    UP000007819    UP000215335    UP000183832    UP000007799    UP000002358    UP000261460    UP000265100    UP000198287    UP000000305    UP000034805    UP000005207    UP000269221    UP000016665    UP000192224    UP000265160    UP000261520    UP000261420    UP000261360    UP000095301    UP000008792    UP000005203    UP000085678    UP000233220    UP000233020    UP000233040    UP000265180    UP000095300    UP000009192    UP000075883    UP000092553    UP000001070    UP000261560    UP000265080    UP000076502    UP000001519    UP000240080    UP000002277    UP000007303    UP000079169    UP000005640    UP000092443    UP000007798    UP000242457    UP000092460    UP000265200    UP000028761   

PRIDE

Q2F6C9    S6B5B9    Q03393   

Pfam

PF01242   PTPS

Interpro

IPR022470   PTPS_Cys_AS        + More
IPR022469   PTPS_His_AS       
IPR007115   6-PTP_synth/QueD       
IPR038418   6-PTP_synth/QueD_sf       

Gene 3D

3.30.479.10    

ProteinModelPortal

Q2F6C9   D4AH60   A0A0N1IHA7   I4DRI8   S6B5B9   A0A0L7LSC6   + More
A0A2W1BU92   A0A2A4J807   S4PGH6   A0A212F955   A0A2H1WJT7   A0A182JLQ5   A0A182PVX2   A0A182YLP1   A0A182VTH8   A0A182NBB4   A0A182R415   A0A182KWH9   A0A182VPH3   A0A182ULF8   A0A182X683   Q7Q899   A0A182IBA4   Q17KM3   A0A182QSC5   A0A182H841   A0A084VMJ8   A0A023EI03   A0A2M4C170   A0A2M3ZIZ8   A0A1Q3FHK5   A0A2M4AMY5   W5JQR8   A0A182F3S5   A0A336LQR4   A0A067R9T2   A0A182K4W8   A0A2S2QB83   A0A0P5IV57   A0A0P4ZCR6   A0A0P4Z638   J9K2U2   A0A2S2PTB2   A0A2H8THS1   A0A232FNL9   A0A1J1I5E0   F2UQD7   A0A0K8TLH4   K7ITD3   A0A3B4FLU6   A0A0A9YN07   A0A3P8R1N2   A0A226ENK5   E9FSQ0   A0A0P7UT15   I3KU31   A0A3M0ISX8   U3JUN8   A0A1W5AV16   A0A3P9B7U4   A0A3B4A070   A0A310SFP7   A0A3B4U8Z4   A0A3B4YMX5   A0A1L8EFS2   A0A1L8EFN2   A0A1I8NER8   B4M9L7   A0A088AJ91   A0A1S3HTE3   R4WJU7   A0A2K6UF50   A0A2K5CU78   A0A2D4LL60   A0A2D4HG29   A0A2K5PQM5   A0A3P9MLS5   A0A1I8QDN9   B4KEP4   A0A182M712   A0A0M4ESB3   B4JQ90   A0A3B3CRS3   A0A3P8TH00   A0A154PNM9   A0A2D4G939   A0A2I2YV86   A0A2R9AR91   H2R4Y8   Q4SSZ9   A0A1S4E8G1   Q03393   A0A1B6K5I9   A0A1A9XL25   B4N7P2   A0A2A3EUT5   A0A1L8DZF6   A0A1B0B7P3   A0A3P9ID22   A0A096NC15  

PDB

3I2B     E-value=1.28799e-45,     Score=456

KEGG

692791    K01737 6-pyruvoyltetrahydropterin/6-carboxytetrahydropterin synthase [EC:4.2.3.12 4.1.2.50] | (RefSe

PATHWAY

00790    Folate biosynthesis - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)

GO

GO:0006729   GO:0003874   GO:0046872   GO:0005739   GO:0042803   GO:0006728   GO:0005737   GO:0051186   GO:0005829   GO:0006520   GO:0042802   GO:0007417   GO:0016811   GO:0005524   GO:0004672   GO:0007169   GO:0016020   GO:0004713   GO:0006281   GO:0048384  

PANTHER

PTHR12589  

Length:

167

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.00145

Exp number, first 60 AAs:

0.0006

Total prob of N-in:

0.02633

outside

1  -  167

 

 

Pi

121.100826

Theta

200.366819

Tajima's D

-1.313045

CLR

215.758196

CSRT

0.0864956752162392

Interpretation

Uncertain