Gene

KWMTBOMO02882  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA003655

Annotation

PREDICTED:_mitochondrial_enolase_superfamily_member_1-like_[Bombyx_mori]

Location

Bomo_Chr5(-):13117685-13126153      View in EpiBrowser

Full name

Mitochondrial enolase superfamily member 1      

Alternative Name

Antisense RNA to thymidylate synthase
L-fuconate dehydratase

Location in the cell

Cytoplasmic   Reliability : 3.559

CDS

ATGCCTGCTCCAAATAGAGATGGTCTGAAAATTGTAGATGTTGACGTTAAGGACGTACGTTTCCCTACATCGCTTGGAGGTCATGGATCTGACGCTGTGCACACAGATCCAGACTACTCCTGCGCCTACGTCATCATAACCACAGAGAGCGGTAAACGCGGCTATGGACTTACTTTCACCTGCGGTAGAGGCACTGAGATCATAGTCTTCACCATACGATCTTTTAAACGATTTTTGTTGGGCAAGAACGCTTCGGACATTTTTGCCGATTTTGCTGGATTCTGGCGTGCGATTACCAATGAATCACAAATGAGATGGATCGGTCCAGAAAAGGGAGTAACTCATTTGGCAGTTGCAGCCATTGTTAACTCCTTATGGGACTTGTGGGCAAGAATAGAAAACAAGCCACTGTGGCGACTTTTGTCTGATATGGATCCTGTGGAATTGGTATCAACAATAGATTTCAGATACATTACTGACGTAATAACTCCAGAAGATGCAGTGGAATTACTGAGAAGTAAACAAGCTGGGAAAGAAGAAAGAATAGAGTTTTTGTTAGAGAACGGATATCCGTGCTATACTACGCAAGTTGGCTGGTTGGGTTATAGCGATGAATTAGTTGAGGAATTATGTGATAAATATCTGAAACTCGGATTTACCTACTTCAAAGTGAAGGTTGGTCGCAATTTTGAAGATGACTATAGACGATGCAGCGCAGTACGACGGTATATCGGGGACGAAAATGTTTTGATGGTTGATGCCAACCAAGCCTGGAGTGTGGGCCAAGCTATCGAATGGATGAAAAAGTTAGCGCCTTTGAAGCCAATGTGGATCGAAGAGCCAACTTCTCCTGATGACGTCTTAGGACATGCAACAATCTCAGAGGCCCTAAAACCTTATGGTATTGGAGTAGCCACAGGCGAAATGTGCGCCAATCGAGTACTTTTTAAACAATTCCTGCAGAGCGGCGGTATGCAGTACTGTCAAATAGACGCGGCCAGAATCGGTGGTGTCAATGAAATATTAGCGGTGTATCTTATGGCTGAAAAACTTAGAGTAAAAGTATGTCCACACGCCGGAGGAGTTGGTTTGTGTGAGATGGTGCAACACCTTCAATTCTGGGATTACGCGAGCGTGTCTTGTACGATGGAGGATCGCCTCATAGAGTACATTGATCAACAACACGAGCATTTTATAGATCCATGTGTCGTTGAAAATGCGAAATATTTAGCACCTCAATTACCAGGATACAGCACACAGTTCCTGCAAGAGACTCTCGAAAAGTATACGTATCCACGTGGAACGGAATGGGAAAAGATGATCAAAGAAGGAATTTTCCCTCAACCCGACGAAACCGAATGGGCGAACCCTTAA

Protein

MPAPNRDGLKIVDVDVKDVRFPTSLGGHGSDAVHTDPDYSCAYVIITTESGKRGYGLTFTCGRGTEIIVFTIRSFKRFLLGKNASDIFADFAGFWRAITNESQMRWIGPEKGVTHLAVAAIVNSLWDLWARIENKPLWRLLSDMDPVELVSTIDFRYITDVITPEDAVELLRSKQAGKEERIEFLLENGYPCYTTQVGWLGYSDELVEELCDKYLKLGFTYFKVKVGRNFEDDYRRCSAVRRYIGDENVLMVDANQAWSVGQAIEWMKKLAPLKPMWIEEPTSPDDVLGHATISEALKPYGIGVATGEMCANRVLFKQFLQSGGMQYCQIDAARIGGVNEILAVYLMAEKLRVKVCPHAGGVGLCEMVQHLQFWDYASVSCTMEDRLIEYIDQQHEHFIDPCVVENAKYLAPQLPGYSTQFLQETLEKYTYPRGTEWEKMIKEGIFPQPDETEWANP

Description

Plays a role in the catabolism of L-fucose, a sugar that is part of the carbohydrates that are attached to cellular glycoproteins. Catalyzes the dehydration of L-fuconate to 2-keto-3-deoxy-L-fuconate by the abstraction of the 2-proton to generate an enediolate intermediate that is stabilized by the magnesium ion (PubMed:24697329).
Plays a role in the catabolism of L-fucose, a sugar that is part of the carbohydrates that are attached to cellular glycoproteins. Catalyzes the dehydration of L-fuconate to 2-keto-3-deoxy-L-fuconate by the abstraction of the 2-proton to generate an enediolate intermediate that is stabilized by the magnesium ion. May down-regulate thymidylate synthase activity, possibly already at the RNA level, by promoting the degradation of TYMS mRNA via an antisense RNA-based mechanism.

Catalytic Activity

L-fuconate = 2-dehydro-3-deoxy-L-fuconate + H2O

Cofactor

Mg(2+)

Biophysicochemical Properties

0.2 mM for L-fuconate
3.0 mM for L-galactonate
2.0 mM for D-arabinonate
4.0 mM for L-arabinonate
0.4 mM for D-ribonate

Similarity

Belongs to the mandelate racemase/muconate lactonizing enzyme family. ENOSF1 subfamily.

Keywords

3D-structure   Alternative splicing   Complete proteome   Isomerase   Lyase   Magnesium   Metal-binding   Mitochondrion   Phosphoprotein   Polymorphism   Reference proteome   Ubl conjugation  

Feature

chain  Mitochondrial enolase superfamily member 1
splice variant  In isoform 7.
sequence variant  In dbSNP:rs34724061.

Uniprot

H9J2B8   A0A2A4J6F1   A0A194QT29   A0A2H1VUA7   A0A194PQI3   A0A212F2J7   + More
A0A1Y1LB49   A0A232EVR7   K7IP03   A0A1L8E090   V5GXH9   A0A088AP94   J3JUQ4   Q17LB3   A0A2A3EQK7   U4UAK6   A0A182XYT3   A0A146MHV5   A0A2J7RA94   A0A2J7RA99   A0A0A9W9Y6   A0A067R9Q4   B0X3G3   A0A154PQK6   A0A1C7CZ92   A0A1Q3FQ14   R4WII9   A0A1B6MKM5   A0A1B6MV81   A0A1B6MJ12   D6X2D5   E2BEE0   A0A1B6MAI1   A0A2M4CUI5   A0A1B6HZU8   A0A1B6HTZ4   A0A1B6JKH1   A0A1B6K5H2   A0A2M4CVW5   F4W4P6   A0A195CSI5   A0A0P4VYV8   A0A0C9QSM1   A0A336KSX9   A0A146LSZ2   A0A195EUV4   A0A336MJD8   A0A0J7KHD2   A0A087UYV6   A0A224XQ09   A0A023F7X0   A0A1W4X5J5   E0VBK9   A0A1S3J3U1   A0A195E309   A0A226DJ20   A0A3P8XTV6   A0A1S3J3E9   A0A1E1XHK5   A0A2P2I2Q3   R4G3I3   A0A131YXD4   A0A060VV79   R7UGF7   B5X4N7   A0A151WV30   A0A3P8NAI9   E6ZJ39   A0A3P9AWF9   A7RF96   A0A3B4ETY4   A0A2I2Y9Z1   A0A3P8UDU7   A0A2U4AYT3   A0A384AW67   A0A315VFX8   A0A2I3GMM1   A0A2R9A354   H2QE69   A0A2T7NPI3   K1P9M1   A0A2I4BLY8   I3JFM4   H2NVZ6   I3JFM5   A0A341CIA8   G1PE38   A0A3B4BP63   A0A3B4TXM3   Q7L5Y1   A0A3B5R0U4   A0A1A8CGA7   A0A2K6LA53   A0A2Y9ESK1   A0A2Y9PIL4   A0A131XSD3   Q5RAT4   H3B9I9   A0A3P8T915  

EC Number

4.2.1.68

Pubmed

19121390    26354079    22118469    28004739    28648823    20075255    + More
22516182    23537049    17510324    25244985    26823975    25401762    24845553    20966253    23691247    18362917    19820115    20798317    21719571    25474469    20566863    25069045    28503490    26830274    24755649    23254933    20433749    25186727    17615350    22398555    24487278    29703783    22722832    16136131    22992520    21993624    8493092    8764108    14702039    16177791    15489334    8869746    12084460    16162288    15994970    21269460    24275569    24697329    23542700    9215903   

EMBL

BABH01007636    BABH01007637    BABH01007638    BABH01007639    BABH01007640    BABH01007641    + More
NWSH01002675    PCG67697.1    KQ461150    KPJ08678.1    ODYU01004483    SOQ44403.1    KQ459597    KPI94999.1    AGBW02010734    OWR47949.1    GEZM01060813    JAV70892.1    NNAY01001962    OXU22440.1    GFDF01002008    JAV12076.1    GALX01001994    JAB66472.1    APGK01043430    APGK01043431    BT126969    KB741014    AEE61931.1    ENN75465.1    CH477217    EAT47463.1    KZ288193    PBC34073.1    KB632204    ERL90097.1    GDHC01000092    JAQ18537.1    NEVH01006576    PNF37744.1    PNF37740.1    GBHO01041966    GBHO01041965    GBHO01041964    JAG01638.1    JAG01639.1    JAG01640.1    KK852609    KDR20308.1    DS232314    EDS39855.1    KQ435007    KZC13538.1    GFDL01005360    JAV29685.1    AK417240    BAN20455.1    GEBQ01003440    JAT36537.1    GEBQ01000147    JAT39830.1    GEBQ01004030    JAT35947.1    KQ971371    EFA10251.2    GL447768    EFN85985.1    GEBQ01007051    JAT32926.1    GGFL01004802    MBW68980.1    GECU01027507    JAS80199.1    GECU01029558    JAS78148.1    GECU01007930    JAS99776.1    GECU01001004    JAT06703.1    GGFL01004800    MBW68978.1    GL887547    EGI70812.1    KQ977329    KYN03487.1    GDRN01086288    JAI61201.1    GBYB01006734    JAG76501.1    UFQS01000685    UFQT01000685    SSX06193.1    SSX26547.1    GDHC01008374    JAQ10255.1    KQ981954    KYN32040.1    UFQS01001431    UFQT01001431    SSX10854.1    SSX30534.1    LBMM01007310    KMQ89858.1    KK122338    KFM82545.1    GFTR01006203    JAW10223.1    GBBI01001379    JAC17333.1    DS235032    EEB10765.1    KQ979701    KYN19533.1    LNIX01000019    OXA44844.1    GFAC01000426    JAT98762.1    IACF01002515    LAB68166.1    ACPB03005357    GAHY01002180    JAA75330.1    GEDV01004704    JAP83853.1    FR904272    CDQ56894.1    AMQN01007889    KB301649    ELU05310.1    BT046006    ACI34268.1    KQ982718    KYQ51713.1    FQ310508    CBN82073.1    DS469507    EDO49916.1    CABD030107436    NHOQ01002355    PWA18012.1    ADFV01007410    ADFV01007411    ADFV01007412    ADFV01007413    ADFV01007414    ADFV01007415    ADFV01007416    ADFV01007417    ADFV01007418    ADFV01007419    AJFE02052164    AJFE02052165    AJFE02052166    AACZ04065248    AACZ04065249    GABC01001585    GABF01001062    GABD01009342    GABE01010219    NBAG03000249    JAA09753.1    JAA21083.1    JAA23758.1    JAA34520.1    PNI61347.1    PZQS01000010    PVD23085.1    JH823239    JH816947    EKC18083.1    EKC31697.1    AERX01002937    AERX01002938    ABGA01080916    ABGA01080917    ABGA01080918    ABGA01080919    ABGA01080920    ABGA01080921    ABGA01080922    ABGA01080923    ABGA01080924    NDHI03003366    PNJ81173.1    AAPE02044391    AAPE02044392    AF305057    X67098    X89602    AK127818    AK093873    AK127219    AK292780    AP001178    CH471113    BC001285    HADZ01014157    SBP78098.1    GEFM01006691    JAP69105.1    CR858928    AFYH01067708    AFYH01067709    AFYH01067710    AFYH01067711    AFYH01067712    AFYH01067713   

Proteomes

UP000005204    UP000218220    UP000053240    UP000053268    UP000007151    UP000215335    + More
UP000002358    UP000005203    UP000019118    UP000008820    UP000242457    UP000030742    UP000076408    UP000235965    UP000027135    UP000002320    UP000076502    UP000075882    UP000007266    UP000008237    UP000007755    UP000078542    UP000078541    UP000036403    UP000054359    UP000192223    UP000009046    UP000085678    UP000078492    UP000198287    UP000265140    UP000015103    UP000193380    UP000014760    UP000087266    UP000075809    UP000265100    UP000265160    UP000001593    UP000261460    UP000001519    UP000265120    UP000245320    UP000261681    UP000001073    UP000240080    UP000002277    UP000245119    UP000005408    UP000192220    UP000005207    UP000001595    UP000252040    UP000001074    UP000261440    UP000261420    UP000005640    UP000002852    UP000233180    UP000248484    UP000248483    UP000008672    UP000265080   

PRIDE

H9J2B8    A0A088AP94    Q7L5Y1    Q5RAT4   

Pfam

PF02746   MR_MLE_N        + More
PF00531   Death
PF13378   MR_MLE_C

Interpro

IPR029017   Enolase-like_N        + More
IPR011029   DEATH-like_dom_sf       
IPR018110   Mandel_Rmase/mucon_lact_enz_CS       
IPR036849   Enolase-like_C_sf       
IPR013342   Mandelate_racemase_C       
IPR034610   L-fuconate_dehydratase       
IPR029065   Enolase_C-like       
IPR000488   Death_domain       
IPR013341   Mandelate_racemase_N_dom       

SUPFAM

SSF47986   SSF47986        + More
SSF51604   SSF51604       

Gene 3D

3.20.20.120     3.30.390.10    

CDD

cd03324  rTSbeta_L-fuconate_dehydratase

ProteinModelPortal

H9J2B8   A0A2A4J6F1   A0A194QT29   A0A2H1VUA7   A0A194PQI3   A0A212F2J7   + More
A0A1Y1LB49   A0A232EVR7   K7IP03   A0A1L8E090   V5GXH9   A0A088AP94   J3JUQ4   Q17LB3   A0A2A3EQK7   U4UAK6   A0A182XYT3   A0A146MHV5   A0A2J7RA94   A0A2J7RA99   A0A0A9W9Y6   A0A067R9Q4   B0X3G3   A0A154PQK6   A0A1C7CZ92   A0A1Q3FQ14   R4WII9   A0A1B6MKM5   A0A1B6MV81   A0A1B6MJ12   D6X2D5   E2BEE0   A0A1B6MAI1   A0A2M4CUI5   A0A1B6HZU8   A0A1B6HTZ4   A0A1B6JKH1   A0A1B6K5H2   A0A2M4CVW5   F4W4P6   A0A195CSI5   A0A0P4VYV8   A0A0C9QSM1   A0A336KSX9   A0A146LSZ2   A0A195EUV4   A0A336MJD8   A0A0J7KHD2   A0A087UYV6   A0A224XQ09   A0A023F7X0   A0A1W4X5J5   E0VBK9   A0A1S3J3U1   A0A195E309   A0A226DJ20   A0A3P8XTV6   A0A1S3J3E9   A0A1E1XHK5   A0A2P2I2Q3   R4G3I3   A0A131YXD4   A0A060VV79   R7UGF7   B5X4N7   A0A151WV30   A0A3P8NAI9   E6ZJ39   A0A3P9AWF9   A7RF96   A0A3B4ETY4   A0A2I2Y9Z1   A0A3P8UDU7   A0A2U4AYT3   A0A384AW67   A0A315VFX8   A0A2I3GMM1   A0A2R9A354   H2QE69   A0A2T7NPI3   K1P9M1   A0A2I4BLY8   I3JFM4   H2NVZ6   I3JFM5   A0A341CIA8   G1PE38   A0A3B4BP63   A0A3B4TXM3   Q7L5Y1   A0A3B5R0U4   A0A1A8CGA7   A0A2K6LA53   A0A2Y9ESK1   A0A2Y9PIL4   A0A131XSD3   Q5RAT4   H3B9I9   A0A3P8T915  

PDB

4A35     E-value=2.24349e-160,     Score=1452

KEGG

101743996    K18334 L-fuconate dehydratase [EC:4.2.1.68] | (RefSeq) mitochondrial enolase superfamily member 1-li

PATHWAY

00051    Fructose and mannose metabolism - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)

GO

GO:0007165   GO:0009063   GO:0016052   GO:0050023   GO:0000287   GO:0044275   GO:0005739   GO:0016853   GO:0003824   GO:0005681   GO:0016021   GO:0004888   GO:0007166   GO:0045211   GO:0006281   GO:0048384  

PANTHER

PTHR13794:SF58  

Subcellular location

Mitochondrion  

Length:

457

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.1424

Exp number, first 60 AAs:

0.02136

Total prob of N-in:

0.00951

outside

1  -  457

 

 

Pi

260.14472

Theta

183.736628

Tajima's D

1.383611

CLR

0.348544

CSRT

0.76241187940603

Interpretation

Uncertain