Gene

KWMTBOMO02803

Pre Gene Modal

BGIBMGA003507

Annotation

PREDICTED:_DNA_helicase_MCM8_[Amyelois_transitella]

Location

Bomo_Chr5(+):11754048-11767108      View in EpiBrowser

Full name

DNA helicase MCM8      

Alternative Name

Minichromosome maintenance 8

Location in the cell

Nuclear   Reliability : 2.38

CDS

ATGAGACGCACCGGAAGAGGGCGATATCGCAACAACTGGTATCGTTCTAAAAATAAAGGAAACATTAACTCCGATGATTCGAGCCTCAACAACACTTCAACATCCGCAAGGGTCACTAGTACTGCTGGGTCTTCAAGAGCACCAGCTAATCAGCTTCCCATAGTAATATCTGTTGGAACGGATCTTAACAACATATGGAAATTGTACTTTCCGTCCGATGAAAAAGGATCCAATGAAACAGTGGCTAAGCATATTAATAGTTTCAAGCTATATATAAACAAGAATAGGAACTTGTTCGATTTGGAAAAAATAGATCAAACTCACAGCGTACCATTGGATGTGCAGAACTTGCAAAATGATGATGAATTTATTCAAGAGTGGAGTTCCTTTACTACTGACCTTTTTGAGAATCCAGTCACAACATTGTCCACACTAGAATATTGCTTGCATGAGACTTTAAACTGTGCAGTAAAACTAAAAGTGAGAATACTAAATCATCAACCAGTTATACCAATTGCGAGTTTGAAAGTTAATTATTTTGGTAAATTAGTAACAATAAAAGGAACAGTAATAAGAGTGGGCAGTATTGGACTTATTTGCACATCCATGGCGTTCGAATGTTCCAGTTGTCACAGTGTTCAAGCTGTTATTCAGCCACAAGGAGTATTTACTGCTCCGAACTTTTGTCAGAGTTGTAATAGTGGCGTTAAGTTTGAGCCTCTACAGTCCTCGCCGTACACGAACACCACAGATTGGCAGGTTGCAAAAATACAAGAGATCCAGTCTCATAGCATTTCCGGTACTATACCTAGAAGTGTTGAGATTGAGCTCCAAGGAGATTTAGTTGGGACTGCATGTCCAGGAGACGTGCTTTCAGTCACTGGTATAGTACAGGTCCGTGGCGAGAGTAAAGGCGGAGAAGACGGAAAGAGAGCTGCGAGATTGTTGCAGTTATACATTGAAGCTATTTCCATTCACAGTCAGAGGAACTTATCCAATCCGACACTGTCCTTTACTTTAAAGGATTATTATGCAATACAGGAAATTCATGGATCCGACGACGTATTCCGCTTACTAGTTCACTCGCTGTGTCCGACCATCTTTGGTCATGAAGCTGTGAAGGCCGGCCTTATCCTGGGGTTGCTGGGTGGAACTGAGCATGAGAACGGTCCGAGATCTAATCCTCACATACTCGTTGTTGGAGATCCAGGACTGGGCAAATCGCAGTTGTTACAGGCTGCAGCGCATGCTGCGCCCAGGGGTGTGTATGTGTGTGGAGCTTCGGCATCAGCGGGAGGACTGACAGTGGCATTAGGCAGGGAGGCCGGAGGTGATTTCGCGCTCGAAGCTGGCGCATTAGTTTTGGCTGACAAAGGCGTTTGCTGTATTGACGAACTAGATAAAATGAAAGCGCATCACAGTAGTCTGCTGGAGGCCATGGAACAGCGGCGTGTGAGTGTTGCCAAGGGAGGTGTGGTGTGCAGCCTAGCCGCTCGGGCCACAGTGCTTGCCGCCGCAAATCCCGCTGCTGGAAGCTATAATAGAGCAAAAACGGTGGTCGAAAATTTGAAACTAAATTCAGCGCTACTTTCGAGGTTTGATCTAGTTTTTATACTTTTGGATCAACCGGATGAGAAAATGGACGCAATGCTCTCTGAACATGTGTTAGCGCTTCACGCTGGTCATAAAACAAAGAAATCGGCACAGGGTAGCAGTTTTAATAGCAGCATGCATACTAGTCAGAGTAATAATAGCAAACGTTCTCTTAGTCAAAGACTCAGACTGAAGCCGGGAGAGGAGATAGACAATTTACCTCTTGTATTGTTACGGAAGTACATAGCTTACGCACAGAGATATGTACATCCGAAATTGAGTAAAGAAGCGGCAACTGCGCTACAGGAATTTTATCTGGAGCTGCGTCACAACCACGACGCGGATCCTAGTGGTGAACTACGGGAAGAGGCAACAGTGGATGATGCTAATGATGTTATTAGCTTAATGAAACACAGCTTGATCGACACCTTTAGCGATGAATTTGGAAACATTCAACTATCTCGGTCTATCAACGGTTCTGGTGTCAGTTCAAAAAGCAAGTTGAAGAAATATTTGGAGGCATTAACAAGACAATCACATCAGTTAGGAAAAGATATTTTTACACGACAAGAGTTGATACAAATACACAAAGCAGCTGGAGTCCAGGGTGACGCAAATGACCTAATTGAAGCAATGCACATACATTCCTACTTGCTTCTTAAAGCATCTAATACATATCAACTTATACCTATATGA

Protein

MRRTGRGRYRNNWYRSKNKGNINSDDSSLNNTSTSARVTSTAGSSRAPANQLPIVISVGTDLNNIWKLYFPSDEKGSNETVAKHINSFKLYINKNRNLFDLEKIDQTHSVPLDVQNLQNDDEFIQEWSSFTTDLFENPVTTLSTLEYCLHETLNCAVKLKVRILNHQPVIPIASLKVNYFGKLVTIKGTVIRVGSIGLICTSMAFECSSCHSVQAVIQPQGVFTAPNFCQSCNSGVKFEPLQSSPYTNTTDWQVAKIQEIQSHSISGTIPRSVEIELQGDLVGTACPGDVLSVTGIVQVRGESKGGEDGKRAARLLQLYIEAISIHSQRNLSNPTLSFTLKDYYAIQEIHGSDDVFRLLVHSLCPTIFGHEAVKAGLILGLLGGTEHENGPRSNPHILVVGDPGLGKSQLLQAAAHAAPRGVYVCGASASAGGLTVALGREAGGDFALEAGALVLADKGVCCIDELDKMKAHHSSLLEAMEQRRVSVAKGGVVCSLAARATVLAAANPAAGSYNRAKTVVENLKLNSALLSRFDLVFILLDQPDEKMDAMLSEHVLALHAGHKTKKSAQGSSFNSSMHTSQSNNSKRSLSQRLRLKPGEEIDNLPLVLLRKYIAYAQRYVHPKLSKEAATALQEFYLELRHNHDADPSGELREEATVDDANDVISLMKHSLIDTFSDEFGNIQLSRSINGSGVSSKSKLKKYLEALTRQSHQLGKDIFTRQELIQIHKAAGVQGDANDLIEAMHIHSYLLLKASNTYQLIPI

Description

Component of the MCM8-MCM9 complex, a complex involved in the repair of double-stranded DNA breaks (DBSs) and DNA interstrand cross-links (ICLs) by homologous recombination (HR). Required for DNA resection by the MRE11-RAD50-NBN/NBS1 (MRN) complex by recruiting the MRN complex to the repair site and by promoting the complex nuclease activity. Probably by regulating the localization of the MNR complex, indirectly regulates the recruitment of downstream effector RAD51 to DNA damage sites including DBSs and ICLs. The MCM8-MCM9 complex is dispensable for DNA replication and S phase progression. However, may play a non-essential for DNA replication: may be involved in the activation of the prereplicative complex (pre-RC) during G(1) phase by recruiting CDC6 to the origin recognition complex (ORC). Probably by regulating HR, plays a key role during gametogenesis. Stabilizes MCM9 protein.
Component of the MCM8-MCM9 complex, a complex involved in the repair of double-stranded DNA breaks (DBSs) and DNA interstrand cross-links (ICLs) by homologous recombination (HR). Required for DNA resection by the MRE11-RAD50-NBN/NBS1 (MRN) complex by recruiting the MRN complex to the repair site and by promoting the complex nuclease activity. Probably by regulating the localization of the MNR complex, indirectly regulates the recruitment of downstream effector RAD51 to DNA damage sites including DBSs and ICLs. The MCM8-MCM9 complex is dispensable for DNA replication and S phase progression. However, may play a non-essential for DNA replication: may be involved in the activation of the prereplicative complex (pre-RC) during G(1) phase by recruiting CDC6 to the origin recognition complex (ORC) (By similarity). Probably by regulating HR, plays a key role during gametogenesis (PubMed:22771120). Stabilizes MCM9 protein (By similarity).
Component of the MCM8-MCM9 complex, a complex involved in homologous recombination repair following DNA interstrand cross-links and plays a key role during gametogenesis. The MCM8-MCM9 complex probably acts as a hexameric helicase required to process aberrant forks into homologous recombination substrates and to orchestrate homologous recombination with resection, fork stabilization and fork restart.
Component of the MCM8-MCM9 complex, a complex involved in the repair of double-stranded DNA breaks (DBSs) and DNA interstrand cross-links (ICLs) by homologous recombination (HR) (PubMed:23401855). Required for DNA resection by the MRE11-RAD50-NBN/NBS1 (MRN) complex by recruiting the MRN complex to the repair site and by promoting the complex nuclease activity (PubMed:26215093). Probably by regulating the localization of the MNR complex, indirectly regulates the recruitment of downstream effector RAD51 to DNA damage sites including DBSs and ICLs (PubMed:23401855). The MCM8-MCM9 complex is dispensable for DNA replication and S phase progression (PubMed:23401855). However, may play a non-essential for DNA replication: may be involved in the activation of the prereplicative complex (pre-RC) during G(1) phase by recruiting CDC6 to the origin recognition complex (ORC) (PubMed:15684404). Probably by regulating HR, plays a key role during gametogenesis (By similarity). Stabilizes MCM9 protein (PubMed:23401855, PubMed:26215093).

Catalytic Activity

ATP + H2O = ADP + H(+) + phosphate

Subunit

Component of the MCM8-MCM9 complex, which forms a hexamer composed of MCM8 and MCM9. Interacts with the DNA mismatch repair (MMR) complex composed at least of MSH2, MSH3, MSH6, PMS1 and MLH1. Interacts with RAD51; the interaction recruits RAD51 to DNA damage sites. Interacts with the MRN complex composed of MRE11, RAD50 and NBN/NBS1. Interacts with CDC6 and ORC2.
Component of the MCM8-MCM9 complex, which forms a hexamer composed of MCM8 and MCM9.
Component of the MCM8-MCM9 complex, which forms a hexamer composed of MCM8 and MCM9 (PubMed:23401855, PubMed:26300262). Interacts with the DNA mismatch repair (MMR) complex composed at least of MSH2, MSH3, MSH6, PMS1 and MLH1 (PubMed:26300262). Interacts with RAD51; the interaction recruits RAD51 to DNA damage sites (PubMed:23401855). Interacts with the MRN complex composed of MRE11, RAD50 and NBN/NBS1 (PubMed:26215093). Interacts with CDC6 and ORC2 (PubMed:15684404).

Similarity

Belongs to the MCM family.

Keywords

ATP-binding   Cell cycle   Chromosome   Complete proteome   DNA damage   DNA repair   DNA replication   DNA-binding   Helicase   Hydrolase   Nucleotide-binding   Nucleus   Phosphoprotein   Reference proteome   Alternative splicing   Disease mutation   Polymorphism   Premature ovarian failure  

Feature

chain  DNA helicase MCM8
splice variant  In isoform 2.
sequence variant  In dbSNP:rs236110.

Uniprot

H9J1X0   A0A194PNB0   A0A2A4JVE4   A0A2H1VYS5   A0A194QTF9   A0A067RG02   + More
A0A2P8YK31   A0A2J7QG22   A0A2J7QG23   A0A1W4WYH4   A0A1B6C6A1   A0A1B6LNA8   A0A1S3JYB5   R7VJZ7   A0A023F0L8   A0A210R1Y1   E0VG28   G3GSC8   A0A1U7QLN4   D6WRH7   A0A0A9VT68   D3ZVK1   A0A0L8GIE4   C3Y0U6   E7F643   Q9CWV1   A0A2U3XVQ8   A0A2Y9GZ20   A0A3B4BP21   A0A2U3VSL3   A0A286XPR6   A0A3N0XLN9   A0A2I0LT18   I0IUP3   A0A091HYU3   A0A091DHE0   A0A093CE50   A0A091U8Y2   U3IHH1   A0A093FBH0   Q9CWV1-2   G1N6W2   A0A1V4IGG3   A0A091LTT4   A0A094KNV4   A0A091W700   A0A091E9I8   A0A091PIE1   H0UTZ0   A0A0A0B0E6   R0L7Q9   A0A093J988   A0A091IJN7   H0YVF5   A0A091V8G2   W5K050   A0A091RNG9   F6RN32   A0A2K6RWZ0   A0A2K5XE27   A0A096NVF9   A0A087VNV0   A0A2U3XVR3   A0A091FR56   A0A1S3ARQ8   A0A2R9AVA6   A0A2I3RCL0   A0A091SXC8   W5UD94   A0A0P6J4W3   A0A2U3VSL2   U3KGL8   A0A093P4V9   A0A287D654   A0A091MUU6   A0A2K5VMR7   A0A2K5ELK4   A0A087R8D1   Q9UJA3   A0A3B1IZ11   G3S967   M3Y4M7   G1LVR9   A0A3B4DI58   K7BP55   G1K8S6   H0X833   G3WA38   A0A2Y9IUV5   A0A093SB49   A0A0D9RLZ3   A0A0Q3PJA2   W5Q9U0   A0A3L8SR85   A0A151MGY3   A0A2Y9RIC7   A0A384D3C7   A0A2J8SU62   A0A1U7TXA8   S4RU69  

EC Number

3.6.4.12

Pubmed

19121390    26354079    24845553    29403074    23254933    25474469    + More
28812685    20566863    21804562    18362917    19820115    25401762    26823975    15057822    18563158    23594743    16141072    19468303    15489334    22771120    21993624    23371554    22771115    15642098    15592404    23749191    20838655    20360741    25329095    17431167    25319552    25362486    22722832    16136131    23127152    12527764    12771218    14702039    11780052    15684404    16325355    18072282    23401855    23186163    26300262    26215093    25437880    22398555    20010809    21709235    20809919    30282656    22293439   

EMBL

BABH01007795    BABH01007796    BABH01007797    BABH01007798    KQ459597    KPI94921.1    + More
NWSH01000604    PCG75380.1    ODYU01004977    SOQ45394.1    KQ461150    KPJ08752.1    KK852659    KDR19137.1    PYGN01000537    PSN44603.1    NEVH01014836    PNF27546.1    PNF27547.1    GEDC01028538    JAS08760.1    GEBQ01014737    JAT25240.1    AMQN01004321    KB293181    ELU16340.1    GBBI01003707    JAC15005.1    NEDP02000845    OWF54901.1    DS235131    EEB12334.1    JH000008    EGV93092.1    KQ971351    EFA06449.1    GBHO01044082    GDHC01004766    JAF99521.1    JAQ13863.1    CH473949    KQ421731    KOF76619.1    GG666479    EEN66079.1    CU929122    FP017149    AK010365    AK133858    AL929562    CH466519    BC046780    BC052070    AAKN02012788    AAKN02012789    RJVU01069573    ROI68668.1    AKCR02000106    PKK20573.1    AB689140    AJ851541    AADN02012184    AADN02012185    KL529797    KFO92403.1    KN124112    KFO22181.1    KL462711    KFV14170.1    KK442053    KFQ70518.1    ADON01070671    KK629635    KFV55042.1    LSYS01009753    OPJ58755.1    KK507393    KFP61837.1    KL263830    KFZ60938.1    KK734780    KFR10875.1    KK718162    KFO54718.1    KK659798    KFQ07425.1    KL873574    KGL99233.1    KB743061    EOB01664.1    KK610049    KFW11565.1    KK500568    KFP08889.1    ABQF01039090    KL410781    KFQ99606.1    KK928228    KFQ43978.1    JSUE03004715    JU477514    AFH34318.1    AHZZ02000595    KL500223    KFO14292.1    KL447392    KFO72985.1    AJFE02073284    AJFE02073285    AJFE02073286    AJFE02073287    AACZ04064981    KK488162    KFQ62635.1    JT412778    AHH39991.1    GEBF01006047    JAN97585.1    AGTO01010954    KL225230    KFW69245.1    AGTP01017661    KK837009    KFP80359.1    AQIA01008254    AQIA01008255    KL226206    KFM09735.1    AJ439063    AY158211    AK027644    AK314654    AL035461    CH471133    BC008830    BC080656    BC101054    BC101055    BC101056    BC101057    CABD030115461    AEYP01029146    AEYP01029147    ACTA01090972    GABC01006556    GABF01006251    GABF01006250    GABE01004056    NBAG03000245    JAA04782.1    JAA15894.1    JAA40683.1    PNI62513.1    PNI62515.1    AAQR03053686    AAQR03053687    AAQR03053688    AAQR03053689    AAQR03053690    AEFK01030381    AEFK01030382    KL670748    KFW80054.1    AQIB01162249    LMAW01002489    KQK81059.1    AMGL01025277    AMGL01025278    AMGL01025279    AMGL01025280    AMGL01025281    AMGL01025282    AMGL01025283    QUSF01000008    RLW06978.1    AKHW03006178    KYO23782.1    NDHI03003544    PNJ24305.1    PNJ24309.1   

Proteomes

UP000005204    UP000053268    UP000218220    UP000053240    UP000027135    UP000245037    + More
UP000235965    UP000192223    UP000085678    UP000014760    UP000242188    UP000009046    UP000001075    UP000189706    UP000007266    UP000002494    UP000053454    UP000001554    UP000000437    UP000000589    UP000245341    UP000248481    UP000261440    UP000245340    UP000005447    UP000053872    UP000000539    UP000028990    UP000016666    UP000001645    UP000190648    UP000053605    UP000052976    UP000053858    UP000053119    UP000007754    UP000053283    UP000018467    UP000006718    UP000233200    UP000233140    UP000028761    UP000053760    UP000079721    UP000240080    UP000002277    UP000221080    UP000016665    UP000054081    UP000005215    UP000233100    UP000233020    UP000053286    UP000005640    UP000001519    UP000000715    UP000008912    UP000001646    UP000005225    UP000007648    UP000248482    UP000053258    UP000029965    UP000051836    UP000002356    UP000276834    UP000050525    UP000248480    UP000261680    UP000189704    UP000245300   

PRIDE

H9J1X0    G3GSC8    D3ZVK1    Q9CWV1    I0IUP3    Q9UJA3    + More

Pfam

PF09412   XendoU        + More
PF05761   5_nucleotid
PF17207   MCM_OB
PF00493   MCM
PF17855   MCM_lid

Interpro

IPR018998   EndoU_C        + More
IPR008380   HAD-SF_hydro_IG_5-nucl       
IPR018525   MCM_CS       
IPR027417   P-loop_NTPase       
IPR033762   MCM_OB       
IPR041562   MCM_lid       
IPR001208   MCM_dom       
IPR023214   HAD_sf       
IPR003593   AAA+_ATPase       
IPR036412   HAD-like_sf       
IPR031327   MCM       
IPR012340   NA-bd_OB-fold       
IPR037227   EndoU-like       

SUPFAM

SSF52540   SSF52540        + More
SSF142877   SSF142877       
SSF56784   SSF56784       
SSF50249   SSF50249       

Gene 3D

3.40.50.1000    

ProteinModelPortal

H9J1X0   A0A194PNB0   A0A2A4JVE4   A0A2H1VYS5   A0A194QTF9   A0A067RG02   + More
A0A2P8YK31   A0A2J7QG22   A0A2J7QG23   A0A1W4WYH4   A0A1B6C6A1   A0A1B6LNA8   A0A1S3JYB5   R7VJZ7   A0A023F0L8   A0A210R1Y1   E0VG28   G3GSC8   A0A1U7QLN4   D6WRH7   A0A0A9VT68   D3ZVK1   A0A0L8GIE4   C3Y0U6   E7F643   Q9CWV1   A0A2U3XVQ8   A0A2Y9GZ20   A0A3B4BP21   A0A2U3VSL3   A0A286XPR6   A0A3N0XLN9   A0A2I0LT18   I0IUP3   A0A091HYU3   A0A091DHE0   A0A093CE50   A0A091U8Y2   U3IHH1   A0A093FBH0   Q9CWV1-2   G1N6W2   A0A1V4IGG3   A0A091LTT4   A0A094KNV4   A0A091W700   A0A091E9I8   A0A091PIE1   H0UTZ0   A0A0A0B0E6   R0L7Q9   A0A093J988   A0A091IJN7   H0YVF5   A0A091V8G2   W5K050   A0A091RNG9   F6RN32   A0A2K6RWZ0   A0A2K5XE27   A0A096NVF9   A0A087VNV0   A0A2U3XVR3   A0A091FR56   A0A1S3ARQ8   A0A2R9AVA6   A0A2I3RCL0   A0A091SXC8   W5UD94   A0A0P6J4W3   A0A2U3VSL2   U3KGL8   A0A093P4V9   A0A287D654   A0A091MUU6   A0A2K5VMR7   A0A2K5ELK4   A0A087R8D1   Q9UJA3   A0A3B1IZ11   G3S967   M3Y4M7   G1LVR9   A0A3B4DI58   K7BP55   G1K8S6   H0X833   G3WA38   A0A2Y9IUV5   A0A093SB49   A0A0D9RLZ3   A0A0Q3PJA2   W5Q9U0   A0A3L8SR85   A0A151MGY3   A0A2Y9RIC7   A0A384D3C7   A0A2J8SU62   A0A1U7TXA8   S4RU69  

PDB

4R7Y     E-value=3.28887e-69,     Score=668

KEGG

101740378    K10737 DNA helicase MCM8 [EC:3.6.4.12] | (RefSeq) DNA helicase MCM8

GO

GO:0004521   GO:0006270   GO:0003677   GO:0005524   GO:0004386   GO:0007292   GO:0097362   GO:0048232   GO:0000724   GO:0003697   GO:0005634   GO:0042555   GO:0003688   GO:0005694   GO:0006974   GO:0007049   GO:0005654   GO:0032407   GO:0003682   GO:0032408   GO:0036298   GO:0071168   GO:0032406   GO:0050821   GO:0019899   GO:0000082   GO:0006260   GO:0003777   GO:0007018   GO:0030286   GO:0005216   GO:0045211   GO:0006281   GO:0048384  

PANTHER

PTHR11630  

Subcellular location

Nucleus   Localizes to nuclear foci. Localizes to double-stranded DNA breaks. Binds chromatin throughout the cell cycle.   With evidence from 2 publications.
Chromosome   Localizes to nuclear foci. Localizes to double-stranded DNA breaks. Binds chromatin throughout the cell cycle.   With evidence from 2 publications.

Length:

762

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.516619999999999

Exp number, first 60 AAs:

0.00357

Total prob of N-in:

0.02527

outside

1  -  762

 

 

Pi

281.425899

Theta

169.479811

Tajima's D

1.124668

CLR

0.606404

CSRT

0.696115194240288

Interpretation

Uncertain