SGID Silkworm Genome Informatics Database
Gene
KWMTBOMO02751  Validated by peptides from experiments
Pre Gene Modal
BGIBMGA003484
Annotation
PREDICTED:_LOW_QUALITY_PROTEIN:_DNA-directed_RNA_polymerase_II_subunit_RPB1_[Bombyx_mori]
Full name
DNA-directed RNA polymerase subunit       + More
DNA-directed RNA polymerase II subunit RPB1      
Alternative Name
DNA-directed RNA polymerase III largest subunit
Location in the cell
Nuclear   Reliability : 3.848
 

Sequence

CDS
ATGGCGACGGCGAATGATTCTAAGGCGCCTTTGCGTCAAGTAAAAAGAGTACAGTTTGGAATACTATCCCCAGATGAAATTCGACGCATGTCAGTTACTGAGGGAGGGATTCGATTCCCGGAGACCATGGAAGGTGGAAGACCGAAGCTTGGCGGTCTTATGGATCCTCGACAAGGGGTAATTGACAGAAGCTCACGGTGTCAAACGTGTGCTGGGAATATGACAGAATGTCCAGGACACTTTGGGCACATTGATTTAGCTAAACCAGTTTTCCATATTGGTTTCATAACAAAAACAATAAAAATACTCAGATGTGTATGCTTTTATTGTTCAAAGCTGCTTGTCAGCCCTACAAATCCAAAGATAAAAGAAGTTATAATGAAATCCAAGGGCCAACCCCGCAAACGGTTGACCTATGTTTATGATCTGTGCAAAGGAAAAAATATATGTGAAGGTGGTGAAGACATGGATATTGGGAAAGAAGGTGAAGAAGGTAAAAGAGGATCTGGTCATGGTGGTTGTGGGCATTATCAACCCTCTATTAGACGACAAGGTCTAGAGCTCACAGCTGAGTGGAAACATGCTAATGAAGATACACAAGAAAAGAAAATTTTTGTCTCTGCAGAAAGAGTATGGGAAATTTTGAAACATATAACAGATGAAGATTCATTTATTTTGGGTATGGATCCAAATTTTGCAAGACCAGATTGGATGATTGTGACGGTTTTGCCTGTCCCTCCATTGTGTGTTCGTCCAGCTGTTGTTATGTTTGGCTCTGCCAAAAATCAAGATGACTTAACCCACAAGCTAGCTGATATAATAAAAGCTAACAATGACTTGGAAAAAAATGAGCAGTCTGGAGCTGCTGCTCATGTCCTTGCAGAGAATATAAGAATGTTACAATTTCATGTTGCTACTTTTGTTGACAATGACATGCCTGGTATGCCAAAAGCTATGCAGAAATCTGGGAAGCCCTTAAAGGCTCTCAAAGCCAGATTGAAAGGAAAAGAAGGAAGAATTCGAGGAAATCTAATGGGGAAACGTGTTGACTTCTCAGCTAGGACAGTAATCACACCTGATCCAAATTTACGTATAGATCAAGTTGGGGTTCCCAGATCAATTGCTCAAAATATGACATTCCCAGAGTTGGTGACTCCATTTAATCTTGATAGAATGCAAGAACTTGTCCGAAGAGGAAATGCACAATACCCTGGGGCAAAGTACATTGTAAGAGAAAACGGAGAAAGAATTGATTTAAGATTCCATCCTAAATCTTCAGATTTACATTTACTCTATGGTTACAAAGTAGAAAGACATCTAAGAGATGATGACCTTGTTATATTTAATCGTCAACCAACTCTTCACAAAATGAGTATGATGGGTCACAGAGTTAAGGTACTTCCTTGGTCAACGTTTCGTATGAATTTGAGTTGCACATCACCTTACAATGCTGATTTTGATGGAGATGAAATGAATTTGCATGTTCCTCAATCAATGGAAACAAGGGCTGAAGTTGAAAATATTCATATAACACCTAGACAAATTATAACTCCACAGGCTAATAAACCTGTAATGGGTATTGTGCAGGACACATTGACTGCCGTTAGAAAAATGACTAAGAGAGATGTTTTTTTAACAAAAGAACAAGTAATGAATCTTCTTATGTTTTTACCTACATGGGATGGTAAAATTCCTCAACCTTGCATTTTGAAACCACAGCCTTTATGGACAGGTAAACAAATATTCACTTTAATAATACCTGGTCCTGTTAATATGATTCGAACTCACTCTACTCATCCTGATGAAGAAGATGATGGTCCTTACAAGTGGATTTCTCCAGGAGATACCAAAGTTGTTGTAGAACATGGAGAACTACTAATGGGTATTTTGTGTAAAAAGTCGCTTGGTGCATCTGCTGGCTCTCTGTTGCATATTTGCATGTTGGAATTAGGCCATGAAACTGCTGGACGATTCTACGGTAACATTCAGACTGTCATTAATAACTGGCTTCTGTTGGAAGGTCACTCAATTGGTATTGGTGACACTATTGCTGATCCACAGACATATCAAGAAATTCAGCGTGCAATCGTAAAAGCTAAAGATGACGTAATAGAAGTCATTCAGAAAGCCCACAATATGGAATTGGAACCCACACCTGGTAATACACTAAGGCAGACCTTTGAAAATCAAGTGAACCGTATCCTCAATGATGCTCGTGATAAAACAGGTGGATCTGCTAAGAAATCATTAACAGAATACAATAATCTCAAAGCTATGGTAGTTGCTGGCTCGAAAGGTTCAAACATTAATATTTCACAAGTCATTGCTTGTGTAGGCCAACAGAATGTTGAAGGAAAACGAATCCCATTTGGATTTAGAAAACGCACCTTACCCCACTTTATTAAAGATGATTATGGTCCTGAATCCAGAGGTTTTGTTGAAAACTCTTATCTAGCTGGTCTGACTCCTTCAGAATTCTATTTTCACGCCATGGGTGGACGTGAAGGTTTAATTGATACTGCTGTAAAAACTGCTGAAACTGGATACATTCAACGGCGTCTCATTAAAGCCATGGAGTCTGTAATGGTCCACTATGATGGTACAGTTAGAAACTCTGTTGGACAACTTATTCAATTGAGGTATGGTGAGGACGGTCTCGCAGGAGAGACAGTAGAATTCCAGAACCTGCCGACTGTTAAATTGTCAAATAAAGCATTTGAAAAGAAATTCAAATTTGACTCTTCCAATGAGAGATATCTGAAGAGAATTTTCAGTGAAGACATAGTCAGAGAGCTAACAGAATCTGGAAGTGTCATAGCTGACCTTGAAAGTGAGTGGGAACAACTTTCTAAAGACAGAGAAACATTACGTCAGATTTTCCCAACGGGAGAATCTAAGGTTGTATTGCCATGTAATTTCAAGAGAATGATTTGGAATGCTCAGAAAACATTCCACATAAACAAGAGAATACCAACAGACTTAAGCCCTATCAAAGTTATAAAAGGTGTATCAGACCTTCTAAAGAAGTGTGTCATTGTAGCTGGTGATGATCGTTTGTCTAAGCAAGCCAATGAGAATGCTACATTACTTTTCCAATGCTTAGTGAGATCAACACTTTGTACAAAATTTGTATCTGAAGAACATAGATTATCTAGTGTAGCATTTGAATGGCTTATCGGGGAAATAGAGGCAAGATTCCAGCAAGCACAAGTAAATCCAGGTGAAATGGTAGGCGCTTTAGCTGCTCAATCACTTGGTGAGCCAGCTACTCAGATGACATTGAATACCTTCCATTTTGCTGGTGTATCATCCAAAAATGTAACACTTGGTGTGCCTCGTCTGAAGGAAATCATCAACATTTCTAAGAAACCAAAGGCTCCTTCTCTAACTGTATTTTTGACCGGTGGAGCAGCCAGAGATGCCGAAAAAGCCAAAAACGTTCTCTGTAGATTAGAACATACAACTTTACGTAAAGTTACTGCTAACACGGCTATTTATTATGACCCTGATCCTCAGAATACTGTTATTGCAGAGGACCAAGAATTTGTCAATGTTTACTACGAAATGCCTGACTTTGATCCTGCGAAAATTTCGCCTTGGTTATTGCGTATTGAACTGGACCGTAAGAGAATGACCGACAAGAAATTAACAATGGAACAGATTGCTGAGAAAATTAATGCTGGTTTTGGTGATGATCTCAATTGTATCTTCAATGATGACAATGCTGAAAAGTTAGTTTTGCGAATTAGAATCATGAATAATGAAGAAAGTAAATTCCAAGATCATGATGAAGAGACAGTAGACAAAATGGAAGATGACATGTTCTTAAGATGTATTGAAGCAAACATGCTGTCTGATATGACTTTGCAAGGCATTGAGGCTATTGCAAAAGTGTATATGCATTTACCACAGACAGAAGCTAAGAAACGTATAACTATAACTGAACAGGGAGAGTTCAAAGCTATAGCAGAATGGTTATTGGAAACAGACGGCACATCTCTTATGAAAGTGCTCTCAGAGCGTGATGTGGATCCAATCAGAACTTTTAGTAACGACATTTGTGAGATATTCCAAGTGCTTGGAATTGAAGCTGTACGAAAATCCGTCGAAAAAGAAATGAATGCGGTTTTGCAGTTCTACGGTCTATACGTGAATTATCGACACTTGGCATTGCTTTGTGACGTCATGACAGCAAAAGGTTATCTTATGGCCATCACACGTCACGGCATTAACCGACAGGACACTGGTGCTCTTATGAGATGTTCATTTGAGGAAACCGTCGATGTTTTATTGGACGCTGCCAGTCATGCTGAAGTGGATCCTATGAGAGGAGTATCAGAAAATATTATAATGGGACAGTTGCCGAGAATGGGTACTGGTTGCTTCGATTTATTACTTGATGGCGAGAAGTGCAAGGAAGCAATGGGCGGTCTTGGAGGCGCAGAAGTGGGAATGTATTTCGGTGTCGGAACGCCATCTATGTCACCACTTATGACTCCCTGGTCTACACAGAATACACCAGGATATGGAAGCAGCGTGTGGTCTCCATGTCAAGTTGGCAGCAGTATGACTCCTGGAGGACCATCATTCTCGCCATCTAACGCATCTGACGCATCAGGACTCTCACCGGCATACAGTAATGCTTGGTCACCACAACCTGGTTCACCAGGATCTCCCGGACCTCCGCTTTCACCTTACTCGTCGCCCGCAGGAGCGTCACCATCTTACTCCCCATCGAATCCTGTTTACGCTCCCGCGTCCCCGAGTATTACTCCAACATCACCACATTATTCACCTGCCAGTCCTTCTTACTCTCCAACTTCACCCAATTATTCCCCGACTTCACCAATATACTCGCCAACATCACCCAGCTACTCGCCAACATCGCCTGGTTATTCGCCCACATCCCCATTATACTCCCCAGCTTCTCCTAGATACTCTCCGTCATCTCCCAACTATTCACCAGCAAGTCCGGCTTATTCAACAACTTCTAATTTCCCGACAACTTCACCTAACTATTCTCCATCAAGTCCTTCGTATTCTCCCACGAGTCTATCGTATTCTCCAACGAGCCCAGGTTATTCCACATCCCCTGGCTATTCGCCCTCGTCACCTAGATATTCACCAAATAGTCCTGTCTACAGTCCTACTTCATCAAATTATTCGCCTTCGTCTCCAAATTACACTTCTGGATCTGTTTATTCACCTACGAGTCCTACATATTCACCTACTTCTCGCTCATATTCTCCATCATCACCGAGCTATTCGCAGTCTTCTCCAAATTATTCACCCTCGTCACCGAATTATTCTCCTACTTCGCCCTCTGTGGCTGGAGGTAGTCCTACATATTCACCAACAAGTCAGCAATATTCTCCAACCAGTCCACAGTATTCTCCGACTAGTCCAGGCTATTCTTCTACATCACAACATCCTAGTAGCACTAGGATCATCAAAATATTCCCCGACATCGCCTACTTTTACTCCTACTTCTCCCAATTATTCTCCGAGCTCTCCTGCGTATTCTTCCACTGGTGGACCATCTACCTATTCTCCAACTTCACCAACATATTCATCTACTTCACCCAATTATGA
Protein
MATANDSKAPLRQVKRVQFGILSPDEIRRMSVTEGGIRFPETMEGGRPKLGGLMDPRQGVIDRSSRCQTCAGNMTECPGHFGHIDLAKPVFHIGFITKTIKILRCVCFYCSKLLVSPTNPKIKEVIMKSKGQPRKRLTYVYDLCKGKNICEGGEDMDIGKEGEEGKRGSGHGGCGHYQPSIRRQGLELTAEWKHANEDTQEKKIFVSAERVWEILKHITDEDSFILGMDPNFARPDWMIVTVLPVPPLCVRPAVVMFGSAKNQDDLTHKLADIIKANNDLEKNEQSGAAAHVLAENIRMLQFHVATFVDNDMPGMPKAMQKSGKPLKALKARLKGKEGRIRGNLMGKRVDFSARTVITPDPNLRIDQVGVPRSIAQNMTFPELVTPFNLDRMQELVRRGNAQYPGAKYIVRENGERIDLRFHPKSSDLHLLYGYKVERHLRDDDLVIFNRQPTLHKMSMMGHRVKVLPWSTFRMNLSCTSPYNADFDGDEMNLHVPQSMETRAEVENIHITPRQIITPQANKPVMGIVQDTLTAVRKMTKRDVFLTKEQVMNLLMFLPTWDGKIPQPCILKPQPLWTGKQIFTLIIPGPVNMIRTHSTHPDEEDDGPYKWISPGDTKVVVEHGELLMGILCKKSLGASAGSLLHICMLELGHETAGRFYGNIQTVINNWLLLEGHSIGIGDTIADPQTYQEIQRAIVKAKDDVIEVIQKAHNMELEPTPGNTLRQTFENQVNRILNDARDKTGGSAKKSLTEYNNLKAMVVAGSKGSNINISQVIACVGQQNVEGKRIPFGFRKRTLPHFIKDDYGPESRGFVENSYLAGLTPSEFYFHAMGGREGLIDTAVKTAETGYIQRRLIKAMESVMVHYDGTVRNSVGQLIQLRYGEDGLAGETVEFQNLPTVKLSNKAFEKKFKFDSSNERYLKRIFSEDIVRELTESGSVIADLESEWEQLSKDRETLRQIFPTGESKVVLPCNFKRMIWNAQKTFHINKRIPTDLSPIKVIKGVSDLLKKCVIVAGDDRLSKQANENATLLFQCLVRSTLCTKFVSEEHRLSSVAFEWLIGEIEARFQQAQVNPGEMVGALAAQSLGEPATQMTLNTFHFAGVSSKNVTLGVPRLKEIINISKKPKAPSLTVFLTGGAARDAEKAKNVLCRLEHTTLRKVTANTAIYYDPDPQNTVIAEDQEFVNVYYEMPDFDPAKISPWLLRIELDRKRMTDKKLTMEQIAEKINAGFGDDLNCIFNDDNAEKLVLRIRIMNNEESKFQDHDEETVDKMEDDMFLRCIEANMLSDMTLQGIEAIAKVYMHLPQTEAKKRITITEQGEFKAIAEWLLETDGTSLMKVLSERDVDPIRTFSNDICEIFQVLGIEAVRKSVEKEMNAVLQFYGLYVNYRHLALLCDVMTAKGYLMAITRHGINRQDTGALMRCSFEETVDVLLDAASHAEVDPMRGVSENIIMGQLPRMGTGCFDLLLDGEKCKEAMGGLGGAEVGMYFGVGTPSMSPLMTPWSTQNTPGYGSSVWSPCQVGSSMTPGGPSFSPSNASDASGLSPAYSNAWSPQPGSPGSPGPPLSPYSSPAGASPSYSPSNPVYAPASPSITPTSPHYSPASPSYSPTSPNYSPTSPIYSPTSPSYSPTSPGYSPTSPLYSPASPRYSPSSPNYSPASPAYSTTSNFPTTSPNYSPSSPSYSPTSLSYSPTSPGYSTSPGYSPSSPRYSPNSPVYSPTSSNYSPSSPNYTSGSVYSPTSPTYSPTSRSYSPSSPSYSQSSPNYSPSSPNYSPTSPSVAGGSPTYSPTSQQYSPTSPQYSPTSPGYSSTSQHPSSTRIIKIFPDIAYFYSYFSQLFSELSCVFFHWWTIYLFSNFTNIFIYFTQL

Summary

Description
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing (By similarity).
Catalytic Activity
a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)
Subunit
Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits.
Miscellaneous
The binding of ribonucleoside triphosphate to the RNA polymerase II transcribing complex probably involves a two-step mechanism. The initial binding seems to occur at the entry (E) site and involves a magnesium ion temporarily coordinated by three conserved aspartate residues of the two largest RNA Pol II subunits. The ribonucleoside triphosphate is transferred by a rotation to the nucleotide addition (A) site for pairing with the template DNA. The catalytic A site involves three conserved aspartate residues of the RNA Pol II largest subunit which permanently coordinate a second magnesium ion.
Similarity
Belongs to the RNA polymerase beta' chain family.
Keywords
Complete proteome   DNA-binding   DNA-directed RNA polymerase   Magnesium   Metal-binding   Nucleotidyltransferase   Nucleus   Phosphoprotein   Reference proteome   Repeat   Transcription   Transferase   Zinc  
Feature
chain  DNA-directed RNA polymerase subunit
EC Number
2.7.7.6
EMBL
BABH01007978    BABH01007979    NWSH01000300    PCG77627.1    ODYU01006685    SOQ48754.1    + More
KQ460207    KPJ16760.1    KQ459597    KPI94870.1    AGBW02008321    OWR53673.1    AB596931    BAJ78684.1    JTDY01000311    KOB77756.1    AB596932    BAJ78685.1    AB596924    BAJ78677.1    KZ288466    PBC25443.1    KQ435878    KOX70044.1    AB596934    BAJ78687.1    GFDF01008612    JAV05472.1    AB596908    BAJ78661.1    KK107078    EZA60388.1    KQ976725    KYM76536.1    ADTU01016112    QOIP01000011    RLU17199.1    GL888255    EGI63788.1    GL438389    EFN69028.1    GL762111    EFZ22137.1    GBXI01005924    JAD08368.1    GAMC01015024    JAB91531.1    GBYB01000787    JAG70554.1    AB596907    BAJ78660.1    CH954180    EDV46036.1    GL451254    EFN79616.1    CM000162    EDX01960.1    GANO01000894    JAB58977.1    CH379063    EAL32621.1    AB596933    BAJ78686.1    BT100316    ACZ54678.1    BT031343    ABY21756.1    CH902621    EDV44455.1    Y18878    AJ547806    AJ548510    AJ548511    AJ548512    AJ548513    AJ548514    AJ548515    AJ548516    AJ548517    AJ548518    AJ548519    AJ548520    AJ548521    AJ548522    AJ548523    AJ548524    AJ548525    AJ548526    AJ548527    AJ548528    AJ548529    AJ548530    AJ548531    AJ548532    OUUW01000021    CAB42795.1    CAD68001.1    CAD70679.1    CAD70680.1    CAD70681.1    CAD70682.1    CAD70683.1    CAD70684.1    CAD70685.1    CAD70686.1    CAD70687.1    CAD70688.1    CAD70689.1    CAD70690.1    CAD70691.1    CAD70692.1    CAD70693.1    CAD70694.1    CAD70695.1    CAD70696.1    CAD70697.1    CAD70698.1    CAD70699.1    CAD70700.1    CAD70701.1    SPP89508.1    M27431    AE014298    M14203    M11798    M19537    AF272643    AF272644    AF272645    AF272646    AF272647    AF272648    AF272649    AF272650    AF272651    AF272652    AF272653    Y18876    AAF89193.1    AAF89194.1    AAF89195.1    AAF89196.1    AAF89197.1    AAF89198.1    AAF89199.1    AAF89200.1    AAF89201.1    AAF89202.1    AAF89203.1    CAB42800.1    CH480851    EDW51400.1    AB596923    BAJ78676.1    Y18877    CAB42796.1    CH933812    EDW06178.1    AB596905    BAJ78658.1    KA647217    AFP61846.1    CH916376    EDV95382.1    JRES01001679    KNC21002.1    CH964239    EDW81831.1    CH940655    EDW66298.1    CCAG010014276    JXJN01019589    GFDL01012955    JAV22090.1    CP012528    ALC48227.1    AB596904    BAJ78657.1    GU060489    ADO24381.1    CH477223    EJY57389.1    JXJN01007805    JXJN01007806    NNAY01002304    OXU21581.1    AB596912    BAJ78665.1    GU060492    ADO24384.1    JXUM01028604    JXUM01028605    KQ560826    KXJ80723.1    AB596913    BAJ78666.1    AB596915    BAJ78668.1    AB596914    BAJ78667.1    KQ977622    KYN01316.1    NEVH01001355    PNF42834.1    UFQT01000381    SSX23771.1    KQ982813    KYQ50333.1    AB596910    BAJ78663.1    GGFK01001684    MBW35005.1    ADMH02000216    ETN67346.1    KQ978957    KYN27201.1    KQ981636    KYN38776.1    GU060491    ADO24383.1    AXCN02000604    GDAI01002193    JAI15410.1    AB596906    BAJ78659.1    AXCM01001126    AB596917    BAJ78670.1    CH479204    EDW30635.1    KK852425    KDR24158.1    KQ434896    KZC10731.1    AB596911    BAJ78664.1    AB596909    BAJ78662.1   
Pfam
PF04992   RNA_pol_Rpb1_6        + More
PF04983   RNA_pol_Rpb1_3
PF04997   RNA_pol_Rpb1_1
PF05000   RNA_pol_Rpb1_4
PF04998   RNA_pol_Rpb1_5
PF04990   RNA_pol_Rpb1_7
PF00623   RNA_pol_Rpb1_2
PF05001   RNA_pol_Rpb1_R
Interpro
IPR007066   RNA_pol_Rpb1_3        + More
IPR007080   RNA_pol_Rpb1_1       
IPR007081   RNA_pol_Rpb1_5       
IPR042102   RNA_pol_Rpb1_3_sf       
IPR000722   RNA_pol_asu       
IPR038120   Rpb1_funnel_sf       
IPR000684   RNA_pol_II_repeat_euk       
IPR007073   RNA_pol_Rpb1_7       
IPR038593   RNA_pol_Rpb1_7_sf       
IPR006592   RNA_pol_N       
IPR007075   RNA_pol_Rpb1_6       
IPR007083   RNA_pol_Rpb1_4       
PDB
6O9L     E-value=0,     Score=6469

Ontologies

Topology

Subcellular location
Nucleus  
Length:
1863
Number of predicted TMHs:
1
Exp number of AAs in TMHs:
19.27095
Exp number, first 60 AAs:
0
Total prob of N-in:
0.00130
outside
1  -  1840
TMhelix
1841  -  1860
inside
1861  -  1863
 
 

Population Genetic Test Statistics

Pi
333.251097
Theta
233.045615
Tajima's D
0.815816
CLR
0.005205
CSRT
0.609719514024299
Interpretation
Uncertain
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