Gene

KWMTBOMO02732  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA003474

Annotation

PREDICTED:_casein_kinase_2_alpha_subunit_isoform_X1_[Bombyx_mori]

Location

Bomo_Chr5(+):9745371-9748575      View in EpiBrowser

Full name

Casein kinase II subunit alpha      

Location in the cell

Cytoplasmic   Reliability : 2.94

CDS

ATGGCAGTACCTAGTAGAGCGAGGGTCTACGCTGATGTGAACTCACAACGTCCAAGGGAATATTGGGATTACGAAAGTTATGTAGTTGACTGGGGCAACCAAGAAGACTATCAGTTGGTTCGCAAGCTGGGTCGCGGGAAATACAGTGAAGTATTTGAAGCAATAAATATTACGAATAATGAAAAATGTGTAGTTAAAATATTAAAGCCTGTAAAAAAGAAGAAAATTAAAAGAGAAATAAAAATTTTAGAAAACTTAAGAGGAGGCACTAATATAATATCTTTACAAGCTGTAGTCAAAGACCCTGTCTCGCGTACACCTGCACTTATATTTGAACATGTGAACAATACTGACTTTAAACAGCTATATTCGACATTGTCAGATTATGATATAAGATACTACTTATATGAGCTTTTAAAGGCATTAGATTATTGCCATAGTATGGGGATAATGCATAGGGATGTGAAACCTCATAATGTGATGATTGACCATGAACATAGAATGTTACGCCTTATTGACTGGGGGTTAGCTGAGTTCTACCATCCGGGGCAAGATTATAATGTTCGTGTTGCTTCTAGATACTTTAAGGGCCCTGAACTTTTGGTAGATTATCAAATGTATGATTATTCATTGGATATGTGGTCACTAGGATGTATGTTGGCATCCATGATTTTCCGTAAAGAGCCATTCTTTCATGGCCATGATAATTATGACCAGCTTGTACGTATTGCGAAAGTACTGGGTACAGAAGAATTGTTTGAGTACTTGGATAAATATCATATAGAACTGGATCCTCGGTTTAATGACATACTTGGCAGACACTCACGTAAGAGATGGGAGCGATTTATACATTCAGAAAATCAACATCTTGTATCACCAGAGGCACTGGACTTTCTTGACCGTTTACTGCGTTATGATCATTATGAACGCTACACTGCTCGTGAAGCTATGGACCATCCATATTTTTGTAAGTTTTTTTTTTTTGTTGTTGTTTAG

Protein

MAVPSRARVYADVNSQRPREYWDYESYVVDWGNQEDYQLVRKLGRGKYSEVFEAINITNNEKCVVKILKPVKKKKIKREIKILENLRGGTNIISLQAVVKDPVSRTPALIFEHVNNTDFKQLYSTLSDYDIRYYLYELLKALDYCHSMGIMHRDVKPHNVMIDHEHRMLRLIDWGLAEFYHPGQDYNVRVASRYFKGPELLVDYQMYDYSLDMWSLGCMLASMIFRKEPFFHGHDNYDQLVRIAKVLGTEELFEYLDKYHIELDPRFNDILGRHSRKRWERFIHSENQHLVSPEALDFLDRLLRYDHYERYTAREAMDHPYFCKFFFFVVV

Description

Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Regulates numerous cellular processes, such as cell cycle progression, apoptosis and transcription, as well as viral infection. May act as a regulatory node which integrates and coordinates numerous signals leading to an appropriate cellular response. During mitosis, functions as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage. Also required for p53/TP53-mediated apoptosis, phosphorylating 'Ser-392' of p53/TP53 following UV irradiation. Can also negatively regulate apoptosis. Phosphorylates the caspases CASP9 and CASP2 and the apoptotic regulator NOL3. Phosphorylation protects CASP9 from cleavage and activation by CASP8, and inhibits the dimerization of CASP2 and activation of CASP8. Regulates transcription by direct phosphorylation of RNA polymerases I, II, III and IV. Also phosphorylates and regulates numerous transcription factors including NF-kappa-B, STAT1, CREB1, IRF1, IRF2, ATF1, SRF, MAX, JUN, FOS, MYC and MYB. Phosphorylates Hsp90 and its co-chaperones FKBP4 and CDC37, which is essential for chaperone function. Regulates Wnt signaling by phosphorylating CTNNB1 and the transcription factor LEF1. Acts as an ectokinase that phosphorylates several extracellular proteins. Phosphorylates PML at 'Ser-565' and primes it for ubiquitin-mediated degradation. Plays an important role in the circadian clock function by phosphorylating ARNTL/BMAL1 at 'Ser-90' which is pivotal for its interaction with CLOCK and which controls CLOCK nuclear entry. Phosphorylates CCAR2 at 'Thr-454' (By similarity).
Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Regulates numerous cellular processes, such as cell cycle progression, apoptosis and transcription, as well as viral infection. May act as a regulatory node which integrates and coordinates numerous signals leading to an appropriate cellular response. During mitosis, functions as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage. Also required for p53/TP53-mediated apoptosis, phosphorylating 'Ser-392' of p53/TP53 following UV irradiation. Can also negatively regulate apoptosis. Phosphorylates the caspases CASP9 and CASP2 and the apoptotic regulator NOL3. Phosphorylation protects CASP9 from cleavage and activation by CASP8, and inhibits the dimerization of CASP2 and activation of CASP8. Regulates transcription by direct phosphorylation of RNA polymerases I, II, III and IV. Also phosphorylates and regulates numerous transcription factors including NF-kappa-B, STAT1, CREB1, IRF1, IRF2, ATF1, SRF, MAX, JUN, FOS, MYC and MYB. Phosphorylates Hsp90 and its co-chaperones FKBP4 and CDC37, which is essential for chaperone function. Regulates Wnt signaling by phosphorylating CTNNB1 and the transcription factor LEF1. Acts as an ectokinase that phosphorylates several extracellular proteins. Phosphorylates PML at 'Ser-565' and primes it for ubiquitin-mediated degradation (By similarity). Plays an important role in the circadian clock function by phosphorylating ARNTL/BMAL1 at 'Ser-90' which is pivotal for its interaction with CLOCK and which controls CLOCK nuclear entry. Phosphorylates CCAR2 at 'Thr-454' (By similarity).

Catalytic Activity

ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]
ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]

Subunit

Tetramer of two alpha and two beta chains.
Tetramer composed of an alpha chain, an alpha' and two beta chains.
Heterotetramer composed of two catalytic subunits (alpha chain and/or alpha' chain) and two regulatory subunits (beta chains). The tetramer can exist as a combination of 2 alpha/2 beta, 2 alpha'/2 beta or 1 alpha/1 alpha'/2 beta subunits. Also part of a CK2-SPT16-SSRP1 complex composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B, which forms following UV irradiation (By similarity). Interacts with RNPS1 (By similarity). Interacts with SNAI1 (PubMed:19923321). Interacts with PML and CCAR2 (By similarity).
Heterotetramer composed of two catalytic subunits (alpha chain and/or alpha' chain) and two regulatory subunits (beta chains). The tetramer can exist as a combination of 2 alpha/2 beta, 2 alpha'/2 beta or 1 alpha/1 alpha'/2 beta subunits. Also part of a CK2-SPT16-SSRP1 complex composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B, which forms following UV irradiation. Interacts with RNPS1, SNAI1, PML and CCAR2 (By similarity).

Miscellaneous

Can use both ATP and GTP as phosphoryl donors. Phosphorylation by casein kinase 2 has been estimated to represent up to one quarter of the eukaryotic phosphoproteome.

Similarity

Belongs to the protein kinase superfamily.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.

Keywords

ATP-binding   Kinase   Nucleotide-binding   Transferase   Wnt signaling pathway   Nucleus   Apoptosis   Biological rhythms   Cell cycle   Complete proteome   Phosphoprotein   Reference proteome   Transcription   Transcription regulation   3D-structure   Direct protein sequencing  

Feature

chain  Casein kinase II subunit alpha

Uniprot

H9J1T7   Q59HV9   A0A2H1WWX2   O76484   A0A2A4JZD8   A0A0L7KL16   + More
S4P9Z6   A0A194RGM1   A0A194PUN6   B0LBZ3   A0A212FIX3   A0A151I5B1   A0A0C9R8G5   E2BH98   E2AW17   A0A0J7KIF9   A0A151J3G8   A0A026VYW4   A0A195C1G2   E0VRT5   A0A2J7RHG1   F4WU82   A0A151JW70   A0A151X092   A0A1B6JP93   A0A067RQK2   A0A2Z5TZA5   A0A1B6DFA8   A0A1B6HBX2   A0A1B6EQA3   A0A2P8XVP2   A0A1B6C1T2   A0A1Y1LNF4   A0A1Y1LQ48   A0A1Y1LHJ1   V5GSL7   D6W7M8   J9JP30   A0A0N0BJQ2   A0A1Y1LJX6   A0A2H8TFK4   A0A087ZW71   A0A2S2QNP6   V9I7J1   A0A2S2PLG9   A0A0L7QV90   A0A2A3EL85   A0A2S2QTX1   A0A0P6IJG4   A0A0N8A556   A0A1W4WNT1   A0A139WPC3   A0A0P5CF09   A0A0N8BMQ4   Q16N28   H9AZT4   A0A154PRR3   A0A1Q3F4A5   A0A0P6DFT2   A0A0P5JST8   A0A0P5GIQ8   H9AZT3   B0WWU1   Q6INV5   A0A2K5Q3H4   A0A023EP31   A0A1L8ETF4   A0A1L8EM01   J3JU18   T1D4A2   A0A2D4HA14   M7B5N8   A0A0P5CHH3   A0A336MN57   P28020   A0A3L8S9E7   W5MKN8   A0A218US37   A0A1V4KKK6   A0A093BP39   H0Z8S4   A0A1S2ZQS3   S7N7R0   A0A076FER8   Q60737   G1KCJ4   Q61177   A0A3L7HE43   H0WLB6   A0A1W7RAV4   P19139   G1N6J4   A0A093LJS6   A0A099Z576   A0A093FU89   A0A093PMS6   A0A091G8J4   A0A091I711   A0A091V228   A0A091MCM8  

EC Number

2.7.11.1

Pubmed

19121390    26227816    23622113    26354079    18184036    18183285    + More
22118469    20798317    24508170    30249741    20566863    21719571    24845553    26760975    29403074    28004739    18362917    19820115    17510324    26483478    24945155    27762356    22516182    24330624    23624526    1544409    30282656    20360741    7846532    16141072    19468303    15489334    10806215    18467326    17954558    21183079    19923321    29704459    8173590    2752008    19330005    12191471    20838655   

EMBL

BABH01007991    AB206394    BAD91393.1    ODYU01011613    SOQ57466.1    AF071210    + More
NWSH01000357    PCG77048.1    JTDY01009137    KOB64058.1    GAIX01003484    JAA89076.1    KQ460207    KPJ16737.1    KQ459597    KPI94850.1    EF554579    ABU49714.1    AGBW02008321    OWR53693.1    KQ976437    KYM86937.1    GBYB01009162    JAG78929.1    GL448287    EFN84867.1    GL443213    EFN62439.1    LBMM01007090    KMQ90052.1    KQ980313    KYN16718.1    KK107591    QOIP01000013    EZA48626.1    RLU15526.1    KQ978350    KYM94682.1    DS235562    EEB16091.1    NEVH01003743    PNF40271.1    GL888353    EGI62250.1    KQ981664    KYN38485.1    KQ982617    KYQ53785.1    GECU01006642    JAT01065.1    KK852527    KDR22024.1    FX985745    BBA93632.1    GEDC01012962    JAS24336.1    GECU01035519    JAS72187.1    GECZ01032459    GECZ01031828    GECZ01029689    GECZ01023360    GECZ01017867    GECZ01010528    GECZ01008514    GECZ01008214    JAS37310.1    JAS37941.1    JAS40080.1    JAS46409.1    JAS51902.1    JAS59241.1    JAS61255.1    JAS61555.1    PYGN01001278    PSN36080.1    GEDC01029815    JAS07483.1    GEZM01055133    GEZM01055130    JAV73116.1    GEZM01055132    GEZM01055128    GEZM01055126    JAV73117.1    GEZM01055127    JAV73122.1    GALX01001302    JAB67164.1    KQ971307    EFA11314.2    ABLF02039862    KQ435713    KOX79291.1    GEZM01055131    GEZM01055129    JAV73118.1    GFXV01001050    MBW12855.1    GGMS01010186    MBY79389.1    JR036295    JR036297    JR036299    JR036301    AEY57056.1    AEY57058.1    AEY57060.1    AEY57062.1    GGMR01017509    MBY30128.1    KQ414727    KOC62558.1    KZ288215    PBC32470.1    GGMS01012013    MBY81216.1    GDIQ01040756    GDIQ01003471    JAN91266.1    GDIP01181489    JAJ41913.1    KYB29773.1    GDIP01171494    JAJ51908.1    GDIQ01162627    JAK89098.1    CH477841    EAT35758.1    EAT35759.1    JXUM01049605    JN967628    KQ561613    AFC78721.1    KXJ77998.1    KQ435078    KZC14427.1    GFDL01012651    JAV22394.1    GDIQ01081251    JAN13486.1    GDIQ01220012    JAK31713.1    GDIQ01247353    JAK04372.1    JN967627    AFC78720.1    DS232152    EDS36202.1    BC072167    AAH72167.1    GAPW01002391    JAC11207.1    CM004482    OCT62569.1    CM004483    OCT60351.1    BT126729    AEE61691.1    GALA01001033    JAA93819.1    IACK01019095    LAA68825.1    KB536575    EMP33256.1    GDIP01171495    JAJ51907.1    UFQS01000459    UFQS01000826    UFQT01000459    UFQT01000826    SSX04203.1    SSX27418.1    X62375    QUSF01000037    RLV98797.1    AHAT01018140    MUZQ01000152    OWK56617.1    LSYS01002950    OPJ84980.1    KN126853    KFU93120.1    ABQF01028338    KE163659    EPQ13026.1    KF516619    AII16523.1    U17112    AK146032    AK158077    AL831735    CH466551    BC026149    BC060742    BC089343    U51866    AAA96795.1    RAZU01000234    RLQ64175.1    AAQR03180014    AAQR03180015    AAQR03180016    AAQR03180017    AAQR03180018    AAQR03180019    AAQR03180020    GFAH01000133    JAV48256.1    L15618    BC091130    J02853    KK606023    KFW09181.1    KL889446    KGL76866.1    KK635004    KFV57919.1    KL670017    KFW77726.1    KL447823    KFO77504.1    KL218292    KFP03997.1    KL410370    KFQ96325.1    KK526524    KFP68324.1   

Proteomes

UP000005204    UP000218220    UP000037510    UP000053240    UP000053268    UP000007151    + More
UP000078540    UP000008237    UP000000311    UP000036403    UP000078492    UP000053097    UP000279307    UP000078542    UP000009046    UP000235965    UP000007755    UP000078541    UP000075809    UP000027135    UP000245037    UP000007266    UP000007819    UP000053105    UP000005203    UP000053825    UP000242457    UP000192223    UP000008820    UP000069940    UP000249989    UP000076502    UP000002320    UP000233040    UP000186698    UP000031443    UP000276834    UP000018468    UP000197619    UP000190648    UP000007754    UP000079721    UP000000589    UP000001646    UP000273346    UP000005225    UP000002494    UP000001645    UP000053641    UP000053258    UP000053760    UP000054308    UP000053283   

PRIDE

H9J1T7    Q59HV9    O76484    P28020    Q60737    Q61177    + More
P19139   

Pfam

PF00069   Pkinase        + More
PF00566   RabGAP-TBC

Interpro

IPR008271   Ser/Thr_kinase_AS        + More
IPR011009   Kinase-like_dom_sf       
IPR017441   Protein_kinase_ATP_BS       
IPR000719   Prot_kinase_dom       
IPR000195   Rab-GTPase-TBC_dom       
IPR035969   Rab-GTPase_TBC_sf       

SUPFAM

SSF56112   SSF56112        + More
SSF47923   SSF47923       

ProteinModelPortal

H9J1T7   Q59HV9   A0A2H1WWX2   O76484   A0A2A4JZD8   A0A0L7KL16   + More
S4P9Z6   A0A194RGM1   A0A194PUN6   B0LBZ3   A0A212FIX3   A0A151I5B1   A0A0C9R8G5   E2BH98   E2AW17   A0A0J7KIF9   A0A151J3G8   A0A026VYW4   A0A195C1G2   E0VRT5   A0A2J7RHG1   F4WU82   A0A151JW70   A0A151X092   A0A1B6JP93   A0A067RQK2   A0A2Z5TZA5   A0A1B6DFA8   A0A1B6HBX2   A0A1B6EQA3   A0A2P8XVP2   A0A1B6C1T2   A0A1Y1LNF4   A0A1Y1LQ48   A0A1Y1LHJ1   V5GSL7   D6W7M8   J9JP30   A0A0N0BJQ2   A0A1Y1LJX6   A0A2H8TFK4   A0A087ZW71   A0A2S2QNP6   V9I7J1   A0A2S2PLG9   A0A0L7QV90   A0A2A3EL85   A0A2S2QTX1   A0A0P6IJG4   A0A0N8A556   A0A1W4WNT1   A0A139WPC3   A0A0P5CF09   A0A0N8BMQ4   Q16N28   H9AZT4   A0A154PRR3   A0A1Q3F4A5   A0A0P6DFT2   A0A0P5JST8   A0A0P5GIQ8   H9AZT3   B0WWU1   Q6INV5   A0A2K5Q3H4   A0A023EP31   A0A1L8ETF4   A0A1L8EM01   J3JU18   T1D4A2   A0A2D4HA14   M7B5N8   A0A0P5CHH3   A0A336MN57   P28020   A0A3L8S9E7   W5MKN8   A0A218US37   A0A1V4KKK6   A0A093BP39   H0Z8S4   A0A1S2ZQS3   S7N7R0   A0A076FER8   Q60737   G1KCJ4   Q61177   A0A3L7HE43   H0WLB6   A0A1W7RAV4   P19139   G1N6J4   A0A093LJS6   A0A099Z576   A0A093FU89   A0A093PMS6   A0A091G8J4   A0A091I711   A0A091V228   A0A091MCM8  

PDB

5ONI     E-value=3.25715e-161,     Score=1457

KEGG

692502    K03097 casein kinase II subunit alpha [EC:2.7.11.1] | (RefSeq) Ck2a, BmCK2a; casein kinase 2 alpha s

PATHWAY

03008    Ribosome biogenesis in eukaryotes - Bombyx mori (domestic silkworm)
04137    Mitophagy - animal - Bombyx mori (domestic silkworm)
04310    Wnt signaling pathway - Bombyx mori (domestic silkworm)

GO

GO:0005524   GO:0004674   GO:0016055   GO:0008353   GO:0016021   GO:0004672   GO:0005634   GO:0018105   GO:0005956   GO:1905818   GO:0000785   GO:0046777   GO:0018107   GO:0016301   GO:0005654   GO:0008284   GO:0005737   GO:0006468   GO:0016581   GO:0008013   GO:0043154   GO:0030307   GO:0045732   GO:0016580   GO:0043021   GO:0042802   GO:0030177   GO:0047485   GO:2000059   GO:1903076   GO:0005886   GO:0006915   GO:0048511   GO:0007049   GO:0019888   GO:0097421   GO:0021987   GO:0033574   GO:0005667   GO:0006631   GO:0006635   GO:0006281   GO:0048384  

Subcellular location

Nucleus  

Length:

331

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.01699

Exp number, first 60 AAs:

0

Total prob of N-in:

0.03847

outside

1  -  331

 

 

Pi

264.024878

Theta

225.30262

Tajima's D

0.49384

CLR

144.314851

CSRT

0.512874356282186

Interpretation

Uncertain