Gene

KWMTBOMO02580  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA002674

Annotation

PREDICTED:_probable_hydroxyacid-oxoacid_transhydrogenase?_mitochondrial_[Bombyx_mori]

Location

Bomo_Chr5(-):6251023-6258938      View in EpiBrowser

Full name

Probable hydroxyacid-oxoacid transhydrogenase, mitochondrial      

Location in the cell

Cytoplasmic   Reliability : 1.046 Mitochondrial   Reliability : 1.419

CDS

ATGACATCAAGAAAACGAATTTTCGATTTATTGCGAACGATAAATATTGCATCTTGCCAATGTCCTGCGCACGGTTACAGAGGAAATATTCAAGTTTCAAATGCAACACCATTAAAAGATTATGCATTTGAGATCAAATGCTCAACAGTTCGATATGGGTTAGGTGTAACTCGTGAAGTAGGCCAGGACTTAGTAAATTTAAATGCTAAAAATGTATGCGTCATGACAGACCCTAATGTTGCATCTTTGAGTCCTATGAAAGCTGTTCTGGATTCACTCACTAGAAATGGAATAAATTATAAAGTTTATGATAAAGTGAGAGTAGAACCTACAGATGGCAGTTTCAAAGATGCAATTCATTTTGCAAAGGAAGGAAACTTTGACAGTTTTGTTGCTATTGGTGGGGGATCAGTGATGGATACTTGTAAAGCTGCCAATTTGTACTATAGTGACCCTGATGCTGAGTTCTTGGATTATGTGAATGCTCCTGTTGGCAAAGGCAAAGCTGTCAATATACAGGTTAAACCAATGATTGCCATACCAACTACGAGCGGCACGGGCAGCGAGACCACCGGCGTCAGCATTTTCGATTACGAAGAAATCCACGCTAAAACCGGTATATCTCATAATGCTCTCCGACCGATACTAGCGTTAATTGATCCGCTTCACACTTTGACAGTGCCCGAGAAAGTATCAAATTATTCTGGTTTCGATATATTTTGCCACGCTTTGGAAAGCTTCACAGCGATACCGTTCACTGAAAGAGGGCCCGCGCCTGCTAATCCGGCTCTCAGACCAGCATATCAAGGCAGCAATCCCATATCTGATGTTTGGGCGCGATTTTGTTTGCAGACCCTGCAAAAGTATTTCGTTCGTTCAGTGAACAATTCTGGAGACGTGGAAGCGAGATCGAGCATGCATCTGGCGGCGACGATGGCGGGCGTCGGCATCGGAAACGCGGGCGTACATTTATGTCACGGGCTAGCGTACCCGATAGCTGGAAACGTTAAGAGTTTTGTTCCCGAGGACTATGGTTCCAATCCGATAATCCCTCACGGCCTGTCGGTTGTAATGACAGCGCCGGCTGTTTTCCGATTCACCGCATCTAGTGACCCTGAGAAACATCTAGAAGCAGCCAGTCTGCTAGGAGCTGACGTCACCAACGCTAAGAGAAAAGACGCCGGTAGAATACTCTCCGACGTCATACTCTTGTATATGGATAAGTTGAAGATTGAAAACGGATTGAAGGCATTAGGATATACTAATGACGACATACCGGACTTGGTCAAGGGTGCTTTACCTCAGGATCGTTTATTAAAAATCGCTCCAGTGACACAATCTGAAGAGGATTTATCAAAATTGCTGGAGGAATCATTGACCATTTACTAA

Protein

MTSRKRIFDLLRTINIASCQCPAHGYRGNIQVSNATPLKDYAFEIKCSTVRYGLGVTREVGQDLVNLNAKNVCVMTDPNVASLSPMKAVLDSLTRNGINYKVYDKVRVEPTDGSFKDAIHFAKEGNFDSFVAIGGGSVMDTCKAANLYYSDPDAEFLDYVNAPVGKGKAVNIQVKPMIAIPTTSGTGSETTGVSIFDYEEIHAKTGISHNALRPILALIDPLHTLTVPEKVSNYSGFDIFCHALESFTAIPFTERGPAPANPALRPAYQGSNPISDVWARFCLQTLQKYFVRSVNNSGDVEARSSMHLAATMAGVGIGNAGVHLCHGLAYPIAGNVKSFVPEDYGSNPIIPHGLSVVMTAPAVFRFTASSDPEKHLEAASLLGADVTNAKRKDAGRILSDVILLYMDKLKIENGLKALGYTNDDIPDLVKGALPQDRLLKIAPVTQSEEDLSKLLEESLTIY

Description

Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2-hydroxyglutarate (D-2-HG). L-3-hydroxybutyrate (L-3-OHB) is also a substrate for HOT when using 2-KG as hydrogen acceptor, resulting in the formation of D-2-HG (By similarity).

Catalytic Activity

(S)-3-hydroxybutanoate + 2-oxoglutarate = (R)-2-hydroxyglutarate + acetoacetate
2-oxoglutarate + 4-hydroxybutanoate = (R)-2-hydroxyglutarate + succinate semialdehyde

Similarity

Belongs to the iron-containing alcohol dehydrogenase family. Hydroxyacid-oxoacid transhydrogenase subfamily.
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Keywords

Complete proteome   Mitochondrion   Oxidoreductase   Reference proteome   Transit peptide  

Feature

chain  Probable hydroxyacid-oxoacid transhydrogenase, mitochondrial

Uniprot

A0A2H1WB69   A0A2A4J8N0   A0A212FLL8   A0A194Q2C9   A0A067QTV8   A0A2J7RB98   + More
E2ABQ4   A0A146KTV5   A0A0A9XR32   D6W8A2   A0A0V0G9S6   E9INY1   A0A026WZP0   A0A2M4ANA6   A0A2R7X5F0   A0A069DTV5   A0A1S3D6P1   F4WH16   A0A1B6M259   A0A182JI21   A0A0P4W3B5   R4FQE2   Q17EN4   A0A182RDJ8   W5JCR3   A0A182FDB3   A0A2K7P695   A0A158NWM4   A0A182WBM3   A0A182M6J0   A0A023F7W9   A0A182QFM5   A0A158NWM5   A0A182NI63   A0A2S2PZR8   A0A151X5L4   A0A195BSZ1   A0A182PDJ5   A0A1B6CDA2   A0A3F2Z247   A0A151K0T9   A0A2S2NN16   A0A182SFB8   Q17EN5   A0A182I4M4   A0A182Y2G2   A0A182V943   Q7Q547   B0WEG0   A0A2A3E6J9   J9KA60   A0A0P4W8T3   A0A1B0CPT7   A0A1B6G2G3   A0A182U1C9   A0A2H8TU81   A0A2L2Y052   E2BXU4   V9KER4   A0A195C8W3   A0A087TZ15   A0A084WMD3   A0A194R770   U5EWU8   A0A154PAN7   A0A1S4F5U5   B4I831   W5MBF6   A0A1S3LXT4   A0A060X9F1   A0A195E351   B3NNM9   A0A1L8E0J8   A0A3P9MJ96   A0A2M4CMK4   A0A3P9I8T3   A0A158NT98   A0A3P8ZT68   H2LR58   G3N9N5   A0A3P9CVV0   B4P7W3   A0A023GNG8   B4QH42   A0A3B3Y1T2   W8BE93   A0A158NT99   A0A3B3TDE4   A0A0L7R4C6   A0A3B0J5U8   A0A146XFT9   A0A1W5ABE9   S5YSM1   I3IVJ4   A0A146XEM5   A0A034WGM7   A0A3B3CP14   B4GHJ1   B4MJH2   Q28XT3  

EC Number

1.1.99.24

Pubmed

22118469    26354079    24845553    20798317    26823975    25401762    + More
18362917    19820115    21282665    24508170    30249741    26334808    21719571    27129103    17510324    20920257    23761445    21347285    25474469    25244985    12364791    26561354    24402279    24438588    17994087    24755649    17554307    25069045    25186727    17550304    22936249    24495485    29240929    25348373    29451363    15632085   

EMBL

ODYU01007382    SOQ50092.1    NWSH01002374    PCG68435.1    AGBW02007743    OWR54631.1    + More
KQ459589    KPI97560.1    KK852945    KDR13466.1    NEVH01005921    PNF38105.1    GL438334    EFN69156.1    GDHC01020037    JAP98591.1    GBHO01020437    GBHO01011259    GBRD01008098    GBRD01007315    JAG23167.1    JAG32345.1    JAG57723.1    KQ971307    EFA10918.1    GECL01001225    JAP04899.1    GL764397    EFZ17696.1    KK107061    QOIP01000014    EZA61308.1    RLU15004.1    GGFK01008943    MBW42264.1    KK856984    PTY26650.1    GBGD01001366    JAC87523.1    GL888148    EGI66495.1    GEBQ01009956    JAT30021.1    GDKW01000646    JAI55949.1    ACPB03009797    GAHY01000444    JAA77066.1    CH477280    ADMH02001892    ETN60619.1    ADTU01028207    AXCM01002363    GBBI01001365    JAC17347.1    AXCN02000396    GGMS01001804    MBY71007.1    KQ982515    KYQ55614.1    KQ976409    KYM90944.1    GEDC01025896    JAS11402.1    KQ981248    KYN44264.1    GGMR01005925    MBY18544.1    EAT44959.1    APCN01002331    AAAB01008960    DS231909    EDS45620.1    KZ288365    PBC26846.1    ABLF02039218    GDRN01081512    JAI62006.1    AJWK01022638    GECZ01017703    GECZ01017586    GECZ01017581    GECZ01016103    GECZ01013142    JAS52066.1    JAS52183.1    JAS52188.1    JAS53666.1    JAS56627.1    GFXV01005695    MBW17500.1    IAAA01006077    IAAA01006078    LAA00485.1    GL451314    EFN79501.1    JW863944    AFO96461.1    KQ978072    KYM97267.1    KK117391    KFM70354.1    ATLV01024425    KE525352    KFB51377.1    KQ460870    KPJ11716.1    GANO01001259    JAB58612.1    KQ434860    KZC08913.1    CH480824    EDW56756.1    AHAT01004377    FR904940    CDQ73949.1    KQ979701    KYN19595.1    CH954179    EDV55586.1    GFDF01001836    JAV12248.1    GGFL01002394    MBW66572.1    ADTU01025758    CM000158    EDW92118.1    GBBM01001008    JAC34410.1    CM000362    CM002911    EDX08188.1    KMY95766.1    GAMC01006945    JAB99610.1    KQ414659    KOC65611.1    OUUW01000001    SPP75082.1    GCES01045799    JAR40524.1    KF439724    AGT15702.1    AERX01045149    GCES01045800    JAR40523.1    GAKP01006024    JAC52928.1    CH479183    EDW35961.1    CH963846    EDW72261.1    CM000071   

Proteomes

UP000218220    UP000007151    UP000053268    UP000027135    UP000235965    UP000000311    + More
UP000007266    UP000053097    UP000279307    UP000079169    UP000007755    UP000075880    UP000015103    UP000008820    UP000075900    UP000000673    UP000069272    UP000005205    UP000075920    UP000075883    UP000075886    UP000075884    UP000075809    UP000078540    UP000075885    UP000076407    UP000078541    UP000075901    UP000075840    UP000076408    UP000075903    UP000007062    UP000002320    UP000242457    UP000007819    UP000092461    UP000075902    UP000008237    UP000078542    UP000054359    UP000030765    UP000053240    UP000076502    UP000001292    UP000018468    UP000087266    UP000193380    UP000078492    UP000008711    UP000265180    UP000265200    UP000265140    UP000001038    UP000007635    UP000265160    UP000002282    UP000000304    UP000261480    UP000261540    UP000053825    UP000268350    UP000192224    UP000005207    UP000261560    UP000008744    UP000007798    UP000001819   

PRIDE

Q7Q547   

Pfam

PF00465   Fe-ADH        + More
PF13855   LRR_8
PF02800   Gp_dh_C
PF00044   Gp_dh_N
PF13516   LRR_6

Interpro

IPR039697   Alcohol_dehydrogenase_Fe        + More
IPR001670   ADH_Fe/GldA       
IPR042157   HOT       
IPR003591   Leu-rich_rpt_typical-subtyp       
IPR001611   Leu-rich_rpt       
IPR032675   LRR_dom_sf       
IPR020828   GlycerAld_3-P_DH_NAD(P)-bd       
IPR020831   GlycerAld/Erythrose_P_DH       
IPR020829   GlycerAld_3-P_DH_cat       
IPR036291   NAD(P)-bd_dom_sf       
IPR020830   GlycerAld_3-P_DH_AS       
IPR006424   Glyceraldehyde-3-P_DH_1       

SUPFAM

SSF51735   SSF51735       

Gene 3D

3.80.10.10    

CDD

cd08190  HOT

ProteinModelPortal

A0A2H1WB69   A0A2A4J8N0   A0A212FLL8   A0A194Q2C9   A0A067QTV8   A0A2J7RB98   + More
E2ABQ4   A0A146KTV5   A0A0A9XR32   D6W8A2   A0A0V0G9S6   E9INY1   A0A026WZP0   A0A2M4ANA6   A0A2R7X5F0   A0A069DTV5   A0A1S3D6P1   F4WH16   A0A1B6M259   A0A182JI21   A0A0P4W3B5   R4FQE2   Q17EN4   A0A182RDJ8   W5JCR3   A0A182FDB3   A0A2K7P695   A0A158NWM4   A0A182WBM3   A0A182M6J0   A0A023F7W9   A0A182QFM5   A0A158NWM5   A0A182NI63   A0A2S2PZR8   A0A151X5L4   A0A195BSZ1   A0A182PDJ5   A0A1B6CDA2   A0A3F2Z247   A0A151K0T9   A0A2S2NN16   A0A182SFB8   Q17EN5   A0A182I4M4   A0A182Y2G2   A0A182V943   Q7Q547   B0WEG0   A0A2A3E6J9   J9KA60   A0A0P4W8T3   A0A1B0CPT7   A0A1B6G2G3   A0A182U1C9   A0A2H8TU81   A0A2L2Y052   E2BXU4   V9KER4   A0A195C8W3   A0A087TZ15   A0A084WMD3   A0A194R770   U5EWU8   A0A154PAN7   A0A1S4F5U5   B4I831   W5MBF6   A0A1S3LXT4   A0A060X9F1   A0A195E351   B3NNM9   A0A1L8E0J8   A0A3P9MJ96   A0A2M4CMK4   A0A3P9I8T3   A0A158NT98   A0A3P8ZT68   H2LR58   G3N9N5   A0A3P9CVV0   B4P7W3   A0A023GNG8   B4QH42   A0A3B3Y1T2   W8BE93   A0A158NT99   A0A3B3TDE4   A0A0L7R4C6   A0A3B0J5U8   A0A146XFT9   A0A1W5ABE9   S5YSM1   I3IVJ4   A0A146XEM5   A0A034WGM7   A0A3B3CP14   B4GHJ1   B4MJH2   Q28XT3  

PDB

4FR2     E-value=1.30999e-22,     Score=264

KEGG

101738633    K11173 hydroxyacid-oxoacid transhydrogenase [EC:1.1.99.24] | (RefSeq) probable hydroxyacid-oxoacid t

GO

GO:0046872   GO:0016491   GO:0005739   GO:0004022   GO:0006539   GO:0047988   GO:0016021   GO:0016620   GO:0050661   GO:0006006   GO:0051287   GO:0006470   GO:0008138   GO:0004725   GO:0006172   GO:0006631   GO:0006635   GO:0006281   GO:0048384   GO:0055114  

PANTHER

PTHR11496   PTHR10836  

Subcellular location

Mitochondrion  

Length:

462

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.25724

Exp number, first 60 AAs:

0.00076

Total prob of N-in:

0.01155

outside

1  -  462

 

 

Pi

238.466773

Theta

198.290298

Tajima's D

0.643332

CLR

0

CSRT

0.554322283885806

Interpretation

Uncertain