Gene

KWMTBOMO02353  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA014412

Annotation

PREDICTED:_probable_malonyl-CoA-acyl_carrier_protein_transacylase?_mitochondrial_[Papilio_machaon]

Location

Bomo_Chr5(+):85386-88243      View in EpiBrowser

Full name

Cytoplasmic tRNA 2-thiolation protein 2       + More
Probable malonyl-CoA-acyl carrier protein transacylase, mitochondrial      

Alternative Name

Bad egg
[Acyl-carrier-protein] malonyltransferase

Location in the cell

Mitochondrial   Reliability : 2.373

CDS

ATGATGAACACGTTCGCAAGAGTAAGTCGAGGTTTAAAAGTGGCACGAAGTTGTCGGACTCGGAGTTCTCGCAGTGAAGATACTCCGTTGCGAAGGTTGCTGGACGAAGCCAGCGCTTTTAATGAAGGTGCTGCAACGGAACCTGAGCTGCGTTGGGCCACTCAGCCGTATGCGGTGCGGCCCAACGAGGGCGATACGAACAAGGCACGTGTGGAGCCCTCGCACACTACCGTTCTGCTGTTTCCAGGCCAGGGCTCGCAACATGTTGGAATGGGCAAGAAACTAATGCATCTGCCCCCAGTAAGAGAACTCTACGAATTAGCCTCTAGCATTGTTGGATGGGATGTGGCTCGAGTTTGCACTGAGGGACCTGAAGAAGAACTGAGTCGACACTGTCAGATCGCCACGTTGGTGACGTCACTAGCGTCCCTCGAACGACTGCGAGACGAGCGACCCTCCGCGCTCGAGCGAGTTCGAGCCGCCGCCGGCTTCTCGCTCGGCGAAATCACGGCGCTCACTTTCGCGGGTGCGCTCACACTCGAGGGCGCGCTGCGGCTGGTCGACATCCGCGCGGCCGCCATGCGCGCCTGCAGCCAAGAGCGTCGCGGCGGCATGCTCACCGTCTGGCTCGGCGCAGACGCCAACCTGTCGCATGCGCTGCTGCGGGCCAGAGAGCACGCGGCCGAGTGCGGCGTGGCGGCGCCCGTGTGCCGCGTCGCCAACTACCTGTACCCGGGCTGCAAGGTGGTGGCGGGCGACGAGGAGGCGCTGCGGTTCGTGGAGCGGCGCGGGCCCGAGTTCGGCGTGCGCAGAGCGGCGCGGGTGCGGGTGGAGGGCGCGTTCCACACGGAGCTGATGGCGCGCGCGGAGGACGCGGTGCGGGCGGCGCTGCACGTGCTGCAGGTGGCGGAGCCGCGGGTGCGCGTGGTGTCGGCCGTGGACGCGCGCCCGTACCGCGACGCGAGGCACGTGCGGGCGCAGCTGGCGCGGCACGTGACGCGGCCGGTGCGCTGGGAGCAGACGCTGCACGTGCTGTACGCGCGGCCGCGGGACCAGCACGTGCCGCTGACGGTCACGTTGGGTCCGGCGGCCGCGCTGCGCTCCACGCTGCGACAGGTCAACGCCCGTGCGTGGGACAGCTCGCTACAAATTCAAGTGTGA

Protein

MMNTFARVSRGLKVARSCRTRSSRSEDTPLRRLLDEASAFNEGAATEPELRWATQPYAVRPNEGDTNKARVEPSHTTVLLFPGQGSQHVGMGKKLMHLPPVRELYELASSIVGWDVARVCTEGPEEELSRHCQIATLVTSLASLERLRDERPSALERVRAAAGFSLGEITALTFAGALTLEGALRLVDIRAAAMRACSQERRGGMLTVWLGADANLSHALLRAREHAAECGVAAPVCRVANYLYPGCKVVAGDEEALRFVERRGPEFGVRRAARVRVEGAFHTELMARAEDAVRAALHVLQVAEPRVRVVSAVDARPYRDARHVRAQLARHVTRPVRWEQTLHVLYARPRDQHVPLTVTLGPAAALRSTLRQVNARAWDSSLQIQV

Description

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by forming a heterodimer with NCS6/CTU1 that ligates sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.
Catalyzes the transfer of a malonyl moiety from malonyl-CoA to the free thiol group of the phosphopantetheine arm of the ACP protein. This suggests the existence of the biosynthesis of fatty acids in mitochondria (By similarity).

Catalytic Activity

holo-[ACP] + malonyl-CoA = CoA + malonyl-[ACP]

Similarity

Belongs to the CTU2/NCS2 family.
Belongs to the type II malonyltransferase family.

Keywords

Complete proteome   Fatty acid biosynthesis   Fatty acid metabolism   Lipid biosynthesis   Lipid metabolism   Mitochondrion   Reference proteome   Transferase   Transit peptide  

Feature

chain  Probable malonyl-CoA-acyl carrier protein transacylase, mitochondrial

Uniprot

H9JXZ4   A0A2H1W9E1   A0A194QAD2   A0A0N0PD17   A0A1W4XH78   U4UHW0   + More
A0A182K6R6   N6TYX1   A0A182Y1A6   A0A182SR97   A0A182LJ29   Q7PNS5   A0A182V0Z4   A0A182XMW5   A0A182HWS1   A0A182TV37   A0A182MQF8   A0A182WIH8   A0A182NGW8   A0A182RFA0   A0A1Y1K8Q2   A0A182QTV4   B0X5U7   W5JD77   A0A182IWV3   A0A182PHM7   A0A1Q3F0X7   A0A2J7Q3E5   A0A084W473   D6WEE0   A0A067QEN1   B0XJD2   Q17JB6   A0A182FHY3   A0A2P8XRG2   A0A336LY83   A0A182H3B5   A0A0A9XEM3   A0A1B0CYL0   A0A224XE70   A0A212FAR0   A0A146LR85   A0A023F858   A0A2R7X533   R4G7V7   A0A1B6D2F3   B3NDD8   A0A1W4W4Z6   A0A0P5WXL7   B4QNE4   A0A0J9RWW5   A0A1A9X0D5   Q8T3L6   Q29EX9   B4H5N9   B4PJY9   A0A0K8UCJ7   E9HLQ8   A0A0P6G5J7   A0A0P5HJ40   A0A0P5TPH4   A0A0N8EDQ1   A0A0A1XKN0   B4N4W8   A0A034VCT6   A0A0P6I0K5   B3M5H3   A0A3B0JSX8   A0A0P5VX29   A0A164Q068   A0A1B0FD88   A0A0P5LWM3   B4LBS4   W8BQ95   A0A1A9YEL0   A0A0P4Z816   B4L989   A0A1B0B1A1   A0A0P5DC40   A0A0P5QWR2   B4J1J4   A0A0P5TDM6   A0A0P4W3S8   A0A2H8TSW3   A0A1A9VN02   A0A0N8ABL1   A0A1I8NRV3   A0A2S2R0G1   A0A0N8A355   T1J2B6   A0A0M4EYU2   A0A2T7NJ82   A0A0P5CR90   A0A0P5CJK4   A0A3B5BC17   A0A1I8MVW5   I3JZP0   A0A3P9B8T0   A0A2U9B2H9   A0A3P9B8V5  

EC Number

2.3.1.39

Pubmed

19121390    26354079    23537049    25244985    20966253    12364791    + More
14747013    17210077    28004739    20920257    23761445    24438588    18362917    19820115    24845553    17510324    29403074    26483478    25401762    22118469    26823975    25474469    17994087    22936249    10731132    12537572    12537569    18245854    15632085    17550304    21292972    25830018    25348373    24495485    25315136    25186727   

EMBL

BABH01041728    BABH01041729    ODYU01007043    SOQ49462.1    KQ459465    KPJ00371.1    + More
KQ460401    KPJ14968.1    KB632169    ERL89465.1    APGK01049566    KB741156    ENN73546.1    AAAB01008960    EAA11696.5    APCN01001535    AXCM01002623    GEZM01089005    JAV57774.1    AXCN02000361    DS232396    EDS41079.1    ADMH02001577    ETN62016.1    GFDL01013907    JAV21138.1    NEVH01019067    PNF23112.1    ATLV01020284    KE525297    KFB45017.1    KQ971318    EFA00362.1    KK853763    KDQ84855.1    DS233486    EDS30220.1    CH477233    EAT46788.1    PYGN01001477    PSN34602.1    UFQS01000040    UFQT01000040    SSW98242.1    SSX18628.1    JXUM01024467    KQ560691    KXJ81258.1    GBHO01025150    JAG18454.1    AJVK01001987    GFTR01005671    JAW10755.1    AGBW02009439    OWR50808.1    GDHC01008286    JAQ10343.1    GBBI01001293    JAC17419.1    KK856560    PTY26025.1    ACPB03000713    GAHY01002052    JAA75458.1    GEDC01017505    JAS19793.1    CH954178    EDV52000.2    GDIP01080145    JAM23570.1    CM000363    EDX10814.1    CM002912    KMZ00184.1    AE014296    AY094770    BT050494    CH379070    EAL29930.2    CH479211    EDW33091.1    CM000159    EDW94758.2    GDHF01028224    JAI24090.1    GL732680    EFX67335.1    GDIQ01056816    JAN37921.1    GDIQ01227295    JAK24430.1    GDIP01235780    GDIP01150102    GDIP01133551    GDIP01126598    GDIP01051114    JAL77116.1    GDIP01185994    GDIP01165190    GDIP01059701    GDIP01047998    GDIP01040734    GDIQ01036860    JAJ37408.1    JAN57877.1    GBXI01003184    JAD11108.1    CH964101    EDW79407.1    GAKP01018653    JAC40299.1    GDIQ01011370    JAN83367.1    CH902618    EDV39583.1    OUUW01000009    SPP85217.1    GDIP01094859    JAM08856.1    LRGB01002490    KZS07311.1    CCAG010017532    GDIQ01167028    JAK84697.1    CH940647    EDW68701.1    GAMC01005348    JAC01208.1    GDIP01216730    JAJ06672.1    CH933816    EDW17264.1    JXJN01007043    GDIP01158253    JAJ65149.1    GDIQ01117942    JAL33784.1    CH916366    EDV95885.1    GDIP01127984    JAL75730.1    GDRN01087120    JAI61075.1    GFXV01005136    MBW16941.1    GDIP01163449    JAJ59953.1    GGMS01014273    MBY83476.1    GDIP01187097    JAJ36305.1    JH431796    CP012525    ALC43737.1    PZQS01000012    PVD21218.1    GDIP01166694    JAJ56708.1    GDIP01169135    JAJ54267.1    AERX01012641    CP026244    AWO98144.1   

Proteomes

UP000005204    UP000053268    UP000053240    UP000192223    UP000030742    UP000075881    + More
UP000019118    UP000076408    UP000075901    UP000075882    UP000007062    UP000075903    UP000076407    UP000075840    UP000075902    UP000075883    UP000075920    UP000075884    UP000075900    UP000075886    UP000002320    UP000000673    UP000075880    UP000075885    UP000235965    UP000030765    UP000007266    UP000027135    UP000008820    UP000069272    UP000245037    UP000069940    UP000249989    UP000092462    UP000007151    UP000015103    UP000008711    UP000192221    UP000000304    UP000091820    UP000000803    UP000001819    UP000008744    UP000002282    UP000000305    UP000007798    UP000007801    UP000268350    UP000076858    UP000092444    UP000008792    UP000092443    UP000009192    UP000092460    UP000001070    UP000078200    UP000095300    UP000092553    UP000245119    UP000261400    UP000095301    UP000005207    UP000265160    UP000246464   

PRIDE

H9JXZ4    Q8T3L6   

Pfam

PF00698   Acyl_transf_1        + More
PF10288   CTU2

Interpro

IPR020801   PKS_acyl_transferase        + More
IPR001227   Ac_transferase_dom_sf       
IPR016035   Acyl_Trfase/lysoPLipase       
IPR016036   Malonyl_transacylase_ACP-bd       
IPR014043   Acyl_transferase       
IPR014729   Rossmann-like_a/b/a_fold       
IPR019407   CTU2       
IPR024925   Malonyl_CoA-ACP_transAc       

SUPFAM

SSF52151   SSF52151        + More
SSF55048   SSF55048       

Gene 3D

3.40.366.10     3.40.50.620    

ProteinModelPortal

H9JXZ4   A0A2H1W9E1   A0A194QAD2   A0A0N0PD17   A0A1W4XH78   U4UHW0   + More
A0A182K6R6   N6TYX1   A0A182Y1A6   A0A182SR97   A0A182LJ29   Q7PNS5   A0A182V0Z4   A0A182XMW5   A0A182HWS1   A0A182TV37   A0A182MQF8   A0A182WIH8   A0A182NGW8   A0A182RFA0   A0A1Y1K8Q2   A0A182QTV4   B0X5U7   W5JD77   A0A182IWV3   A0A182PHM7   A0A1Q3F0X7   A0A2J7Q3E5   A0A084W473   D6WEE0   A0A067QEN1   B0XJD2   Q17JB6   A0A182FHY3   A0A2P8XRG2   A0A336LY83   A0A182H3B5   A0A0A9XEM3   A0A1B0CYL0   A0A224XE70   A0A212FAR0   A0A146LR85   A0A023F858   A0A2R7X533   R4G7V7   A0A1B6D2F3   B3NDD8   A0A1W4W4Z6   A0A0P5WXL7   B4QNE4   A0A0J9RWW5   A0A1A9X0D5   Q8T3L6   Q29EX9   B4H5N9   B4PJY9   A0A0K8UCJ7   E9HLQ8   A0A0P6G5J7   A0A0P5HJ40   A0A0P5TPH4   A0A0N8EDQ1   A0A0A1XKN0   B4N4W8   A0A034VCT6   A0A0P6I0K5   B3M5H3   A0A3B0JSX8   A0A0P5VX29   A0A164Q068   A0A1B0FD88   A0A0P5LWM3   B4LBS4   W8BQ95   A0A1A9YEL0   A0A0P4Z816   B4L989   A0A1B0B1A1   A0A0P5DC40   A0A0P5QWR2   B4J1J4   A0A0P5TDM6   A0A0P4W3S8   A0A2H8TSW3   A0A1A9VN02   A0A0N8ABL1   A0A1I8NRV3   A0A2S2R0G1   A0A0N8A355   T1J2B6   A0A0M4EYU2   A0A2T7NJ82   A0A0P5CR90   A0A0P5CJK4   A0A3B5BC17   A0A1I8MVW5   I3JZP0   A0A3P9B8T0   A0A2U9B2H9   A0A3P9B8V5  

PDB

2C2N     E-value=8.98807e-63,     Score=609

KEGG

101743979    K00645 [acyl-carrier-protein] S-malonyltransferase [EC:2.3.1.39] | (RefSeq) probable malonyl-CoA-acy

PATHWAY

00061    Fatty acid biosynthesis - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)
01212    Fatty acid metabolism - Bombyx mori (domestic silkworm)

GO

GO:0016740   GO:0006633   GO:0005739   GO:0004314   GO:0032447   GO:0016779   GO:0002098   GO:0005737   GO:0034227   GO:0000049   GO:0004312   GO:0003824   GO:0008152   GO:0004713   GO:0005515   GO:0005488   GO:0006289   GO:0004518   GO:0006281   GO:0048384  

Subcellular location

Cytoplasm  
Mitochondrion  

Length:

386

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

1.97256

Exp number, first 60 AAs:

0

Total prob of N-in:

0.10508

outside

1  -  386

 

 

Pi

238.117198

Theta

183.534273

Tajima's D

1.03067

CLR

0.161808

CSRT

0.668716564171791

Interpretation

Uncertain