Gene

KWMTBOMO02326  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA003759

Annotation

PREDICTED:_transcription_elongation_factor_SPT5_isoform_X1_[Papilio_machaon]

Location

Bomo_Chr4(-):17870764-17875071      View in EpiBrowser

Full name

Transcription elongation factor SPT5       + More
S-adenosylmethionine sensor upstream of mTORC1      

Alternative Name

Probable methyltransferase BMT2 homolog
DRB sensitivity-inducing factor large subunit
dSpt5

Location in the cell

Nuclear   Reliability : 2.692

CDS

ATGTCGGATTCGGAAGGTAGTAACTATTCGGGGAGTGGGTCCGAGGCAGGCAGTGCTGTATCAAAACAATCACATCGCAGCGCTGCATCGAATCGGTCGGCAAGATCGCGTTCTATCTCTCGATCAAGGTCCAGGTCGCGTTCTGGAAGCCGTAGCCCCTCTAGGACTCCATCCCGCTCACGATCTAGGTCTCGCTCTAGAAGTCGTTCAGTAGGTTCAGATGCAAGTAAAAATAGAGATGATGAAGCCAAAGAAGCATCTGGCGATGAAGAAGTGGAGGATGAACAAGAGCCCGAAGGTGAGGATTTGGTTGATTCTGAGGAGTATGAAGAAGACGAAGAAGAGGAGAGGCTGAGGAAGAAAAGAAAAAAGGACAGTCGTTATGGTGGATTCATTATTGACGAAGCTGAGGTGGATGATGAAGTTGATGAAGATGACGAATGGGAAGAGGGAGCCCAAGAAATGGGTATTGTAGGAAATGAAGTAGATGAAATTGGGCCAACAGCTAGAGAAATTGAAGGTCGAAGAAGAGGCACTAACCTTTGGGATTCACAGAAAGAGGAGGAGATTGAAGAATATCTTCGAAATAAGTATGCAGATGAATCAGCAGCCCTCAGACACTTTGGTGAAGGTGGAGAAGAGATGTCTGATGAAATAACTCAGCAAACTCTACTACCCGGTATCAAGGATCCAAATTTGTGGATGGTCAGATGTAGGATTGGAGAAGAGAAGGCTACTGTTTTACTTTTGATGCGAAAATTTATTGCTTTCCAATTCTCGGAAGAACCTTTTTTGATAAAGTCAGTTGTAGCACCAGAAGGAGTCAAAGGCTATGTGTACATAGAGGCTTTCAAGCAGACACATGTTAAAAACATTATAGACAAAGTGGGAACCCTTAAGTCTGGAGTGTGGAAACAAGAAATGGTGCCTATTAAGGAAATGACTGATGTTCTCAGAGTAGTCAAAAAGCAATCAGGACTTAAACCGAAACAATGGGTCAGATTAAAACGAGGTTTATACAAAGATGATATTGCTCAAGTTGACTATGTAGACTTGGCACAGAATCAAGTGCATTTGAAACTACTTCCTAGAATAGATTATACAAGACTGAGAGGAGCTTTAAGAACAGTACAGAGCGATAGTGAAGCAGCAAAGCGAAAGAAAAAGAGAAGGCCTCCTGCTAAACCCTTTGATCCAGAGGCAATCCGAGCGATCGGTGGAGAAGTTACTTCCGATGGTGATTTCCTCATATTTGAGGGCAATAGGTACTCTAGGAAAGGATTTCTGTATAAAAACTTTACAATGTCGGCGATACTGTCAGAAGGTGTCAAGCCTTCACTTACAGAGTTGGAAAGATTTGAAGAGCAACCGGAAGGTATCGATATTGAGTTGGCTGCTCCCGCCAAGGACGACCCTGCCGGCTTGCATTCCTTCTCCATGGGTGACAATGTGGAAGTCTGTACTGGAGATCTTGCGAACTTGCAGGCCCGCATCACTGCTATCGACGGTTCGATGATCACTGTCATGCCAAAACACGATGGTCTTAAGGACCCAATTGTCTTCCATCCGAATGAGTTGCGCAAGTACTTCAAAATGGGTGATCACGTGAAAGTGGTGGCTGGGCGTTACGAGGGCGACACAGGCCTCATAGTGCGCGTCGAACCGCACAGGGTCGTGCTTGTTTCCGACCTCACCATGCACGAGCTTGAGGTGCTGCCCCGTGACCTACAGCTCTGTTCAGACATGGCGACTGGCGTTGACTCCCTCGGACAGTTCCAGTGGGGAGACATGGTGCAACTGGATCCACAGACCGTCGGAGTTATCGTGCGCCTCGAGAAAGAGAACTTCCACGTGCTCGGCATGCAGGGCAAGGTCATCGAGTGCAAGCCGCAAGCGCTACAGAAACGCCGCGAGAACCGGTTTACAGTCGCGCTCGATTCCGAGGAGAACACGATTCAGAAGCGCGACATCGTTAAAGTAATCGACGGGCCGCATGCCGGACGCGACGGTGAAATCAAACATCTCTACCGGAACTTCGCCTTCCTCCAGTCGCACATGTACCTCGACAACGGCGGCATCTTCGTGTGCAAGACGCGCCACCTGCAGCTGGCCGGTGGAGCCAGGGGAAATAATGCCACCGCCGCCGGACTCACGCTCGGCTTCATGTCGCCGCGGATAGCTTCTCCTGCACACCCAGGGGCCGGCAGGGGAGGCTCCACCCCTCGCGGTCGTGGGGGCCGAGGCGGCGGCGGAGGTCGAGGCGTCACTCGGGATCGCGAGCTCATTGGCCAAACCATTAAGATCACGGGGGGACCATACAAGGGAAACGTCGGGATCGTCAAAGACGCGACCGGCAGCACCGCGCGCGTCGAGCTGCACTCCTCCTGTCAGACCATCTCCGTGGACCGCGGGCACATCGCCGGGGCGGGGGCCGGGGCTGCGACCGGCGGGGCCTCGTCCTACTCGCGCACGCCGTCCCGCACGCCGGCCGCCGGCGCCGCCACCCCCACGTACCGCGACACGGGCATCAAGACGCCTATGCACGGCGCCGCTACACCCGTCTACGAGGTGGGCTCGCGCACGCCACACTACGGGTCGTCGACCCCGGCGCACGAGGGGTCGCGCACGCCGGCGCACGGGGCCTGGGACCCGAGCGGTGCCGCCACCCCGGCGCGACCGGACTTCGACTACGAGCCGGACGCGTACGGCGCGGGACCGTTCACTCCGCAGACGCCCGGCGCCATGTACGGCTCGGACCACACGTACAGCCCGTACAGTCCGGGCCGGTCGGAGGGCGAGTCGGCGGAGGCGGGTGCGTGGGCGGCACCAGAGCTCGTGGTGCGTCTGAGAGCGGGAGCTGCGGACGGTCTGGCCGCACAGACCGCGGTGGTCCGTGCGACCGGCGGGGGCTCGTGCGCGTTGTACTTACCGGCGGAGGAGCGAGTGGTGTCCGTGCCGCCGGACTTGTTAGAGCCGGTGCTGCCTCAGGCGGGAGAGCGCGTGAAGGTGATCGCGGGAGAGGACCGGGACGCGGTCGGACAACTTATCTCCATCGAAAACCAAGAGGGAGTCGTCAAATTCGGCACCGACGACATCAAAATCATGCAACTACGTCATCTTTGCAAGATGGCCTCTTAA

Protein

MSDSEGSNYSGSGSEAGSAVSKQSHRSAASNRSARSRSISRSRSRSRSGSRSPSRTPSRSRSRSRSRSRSVGSDASKNRDDEAKEASGDEEVEDEQEPEGEDLVDSEEYEEDEEEERLRKKRKKDSRYGGFIIDEAEVDDEVDEDDEWEEGAQEMGIVGNEVDEIGPTAREIEGRRRGTNLWDSQKEEEIEEYLRNKYADESAALRHFGEGGEEMSDEITQQTLLPGIKDPNLWMVRCRIGEEKATVLLLMRKFIAFQFSEEPFLIKSVVAPEGVKGYVYIEAFKQTHVKNIIDKVGTLKSGVWKQEMVPIKEMTDVLRVVKKQSGLKPKQWVRLKRGLYKDDIAQVDYVDLAQNQVHLKLLPRIDYTRLRGALRTVQSDSEAAKRKKKRRPPAKPFDPEAIRAIGGEVTSDGDFLIFEGNRYSRKGFLYKNFTMSAILSEGVKPSLTELERFEEQPEGIDIELAAPAKDDPAGLHSFSMGDNVEVCTGDLANLQARITAIDGSMITVMPKHDGLKDPIVFHPNELRKYFKMGDHVKVVAGRYEGDTGLIVRVEPHRVVLVSDLTMHELEVLPRDLQLCSDMATGVDSLGQFQWGDMVQLDPQTVGVIVRLEKENFHVLGMQGKVIECKPQALQKRRENRFTVALDSEENTIQKRDIVKVIDGPHAGRDGEIKHLYRNFAFLQSHMYLDNGGIFVCKTRHLQLAGGARGNNATAAGLTLGFMSPRIASPAHPGAGRGGSTPRGRGGRGGGGGRGVTRDRELIGQTIKITGGPYKGNVGIVKDATGSTARVELHSSCQTISVDRGHIAGAGAGAATGGASSYSRTPSRTPAAGAATPTYRDTGIKTPMHGAATPVYEVGSRTPHYGSSTPAHEGSRTPAHGAWDPSGAATPARPDFDYEPDAYGAGPFTPQTPGAMYGSDHTYSPYSPGRSEGESAEAGAWAAPELVVRLRAGAADGLAAQTAVVRATGGGSCALYLPAEERVVSVPPDLLEPVLPQAGERVKVIAGEDRDAVGQLISIENQEGVVKFGTDDIKIMQLRHLCKMAS

Description

S-adenosyl-L-methionine-binding protein that acts as an inhibitor of mTORC1 signaling. Acts as a sensor of S-adenosyl-L-methionine to signal methionine sufficiency to mTORC1. Probably also acts as a S-adenosyl-L-methionine-dependent methyltransferase.
Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates transcription elongation by RNA polymerase II. DSIF enhances transcriptional pausing at sites proximal to the promoter, which may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF may also promote transcriptional elongation within coding regions. DSIF is required for the transcriptional induction of heat shock response genes and regulation of genes which control anterior-posterior patterning during embryonic development.

Subunit

Interacts with Spt6. Interacts with Spt4 to form DSIF. DSIF interacts with trx, RNA polymerase II and with the FACT complex, which is composed of dre4/Spt16 and Ssrp/Ssrp1. DSIF can also interact with the exosome, a complex with 3'-5' exoribonuclease activity which is composed of at least Csl4, Dis3, Mtr3, Rrp4, Rrp6, Rrp40, Rrp42, Rrp46 and Ski6. DSIF may also interact with the positive transcription elongation factor b complex (P-TEFb complex), which is composed of Cdk9 and cyclin-T (CycT).

Similarity

Belongs to the SPT5 family.
Belongs to the BMT2 family.

Keywords

Activator   Alternative splicing   Chromosome   Complete proteome   Nucleus   Phosphoprotein   Reference proteome   Repeat   Repressor   Transcription   Transcription regulation  

Feature

chain  Transcription elongation factor SPT5
splice variant  In isoform B.

Uniprot

H9J2M2   A0A2H1V6P2   A0A194QP01   A0A194QGZ7   A0A212EH19   A0A2A4K3K3   + More
K7J5S6   A0A1S4FZD5   A0A0P6IVB5   E2B534   A0A182G1W4   A0A182G3H6   A0A1I8NZL8   A0A195CEG6   A0A154P7Y4   E2A5T8   A0A0M9A996   A0A158NYZ9   A0A026W9W4   F4W9U3   A0A232EKF0   E9IQE3   A0A151WKL0   A0A195BQD0   A0A1B0EUS0   A0A182PF58   A0A084WDR7   A0A336MSR5   A0A182K2J7   U5EVY9   A0A182U6J2   A0A182KYR8   A0NE83   A0A182HQS7   A0A182XJE1   A0A182VIZ1   A0A182RUU0   A0A182M7H4   A0A182YLF7   A0A182N1S7   B4PB84   A0A232EM50   A0A0L7R2I7   A0A182QPP4   A0A182JHT9   A0A1S4J862   B3NJV0   A0A1Q3F0W5   B4MRQ6   A0A182WM74   B4LNW9   B4KME4   B0W6Z7   A0A2M3Z117   A0A182FLB7   W5J4B3   A0A0M3QVL5   Q9V460   W8C3N1   B4HPG3   A0A0J9RGS1   A0A2M4BBB8   A0A1W4VGR7   A0A1J1IGM6   Q292S1   B4GCQ3   A0A3B0J2C6   B3MGG2   A0A2M4BBF7   A0A2M4A8G2   B4J626   A0A034WJE4   A0A0L0CNG6   A0A1B0D500   D2A605   A0A1A9WAC5   A0A1B0FL32   A0A1A9YJP3   A0A1A9UYD5   A0A1B0ARK9   A0A0C9PNW0   A0A0A1WN47   A0A1A9ZRD1   A0A1I8M549   A0A2J7Q156   U4UN14   A0A088A942   E0VS63   A0A2A3E981   A0A0C9QH50   A0A023F4S8   A0A0V0G4H6   A0A069DXS5   A0A0P4VJ54   A0A224XHF9   T1I785   A0A2H8TMI3   A0A2S2Q4T6  

Pubmed

19121390    26354079    22118469    20075255    26999592    20798317    + More
26483478    21347285    24508170    30249741    21719571    28648823    21282665    24438588    20966253    12364791    14747013    17210077    25244985    17994087    17550304    20920257    23761445    11040216    10731132    12537572    12537569    11040217    12490954    12782658    12934007    15380072    15056674    14730313    15741180    17372656    18327897    24495485    22936249    15632085    25348373    26108605    18362917    19820115    25830018    25315136    23537049    20566863    25474469    26334808    27129103   

EMBL

BABH01034508    ODYU01000710    SOQ35934.1    KQ461191    KPJ07079.1    KQ458860    + More
KPJ04782.1    AGBW02014991    OWR40770.1    NWSH01000225    PCG78232.1    AAZX01006342    GDUN01000827    JAN95092.1    GL445701    EFN89194.1    JXUM01138099    JXUM01138100    JXUM01141537    KQ569265    KXJ68689.1    KQ977876    KYM99100.1    KQ434839    KZC07992.1    GL437059    EFN71210.1    KQ435721    KOX78553.1    ADTU01000840    KK107337    QOIP01000002    EZA52451.1    RLU25886.1    GL888033    EGI69079.1    NNAY01003830    OXU18791.1    GL764736    EFZ17213.1    KQ983012    KYQ48347.1    KQ976423    KYM88781.1    AJWK01034746    ATLV01023054    KE525340    KFB48361.1    UFQT01002082    SSX32601.1    GANO01001663    JAB58208.1    AAAB01008948    EAU76692.2    APCN01004686    AXCM01002330    AXCM01002331    CM000158    EDW91497.1    NNAY01003450    OXU19398.1    KQ414666    KOC65090.1    AXCN02000986    CH954179    EDV55209.1    GFDL01013848    JAV21197.1    CH963850    EDW74795.1    CH940648    EDW61138.1    CH933808    EDW09832.1    DS231851    EDS37271.1    GGFM01001445    MBW22196.1    ADMH02002156    ETN58233.1    CP012524    ALC42603.1    AF222864    AE013599    AY069140    BT001668    BT023840    GAMC01002697    JAC03859.1    CH480816    EDW48601.1    CM002911    KMY95116.1    GGFJ01001179    MBW50320.1    CVRI01000047    CRK97601.1    CM000071    EAL24790.1    CH479181    EDW31501.1    OUUW01000001    SPP73143.1    CH902619    EDV35705.1    GGFJ01001180    MBW50321.1    GGFK01003701    MBW37022.1    CH916367    EDW01884.1    GAKP01005039    JAC53913.1    JRES01000153    KNC33767.1    AJVK01025008    AJVK01025009    AJVK01025010    KQ971345    EFA05446.1    CCAG010020342    JXJN01002477    GBYB01002803    JAG72570.1    GBXI01014489    JAC99802.1    NEVH01019959    PNF22323.1    KB632429    ERL95479.1    DS235745    EEB16219.1    KZ288318    PBC28287.1    GBYB01002804    JAG72571.1    GBBI01002728    JAC15984.1    GECL01003176    JAP02948.1    GBGD01000258    JAC88631.1    GDKW01001617    JAI54978.1    GFTR01008536    JAW07890.1    ACPB03000761    GFXV01003336    MBW15141.1    GGMS01003536    MBY72739.1   

Proteomes

UP000005204    UP000053240    UP000053268    UP000007151    UP000218220    UP000002358    + More
UP000008237    UP000069940    UP000249989    UP000095300    UP000078542    UP000076502    UP000000311    UP000053105    UP000005205    UP000053097    UP000279307    UP000007755    UP000215335    UP000075809    UP000078540    UP000092461    UP000075885    UP000030765    UP000075881    UP000075902    UP000075882    UP000007062    UP000075840    UP000076407    UP000075903    UP000075900    UP000075883    UP000076408    UP000075884    UP000002282    UP000053825    UP000075886    UP000075880    UP000008711    UP000007798    UP000075920    UP000008792    UP000009192    UP000002320    UP000069272    UP000000673    UP000092553    UP000000803    UP000001292    UP000192221    UP000183832    UP000001819    UP000008744    UP000268350    UP000007801    UP000001070    UP000037069    UP000092462    UP000007266    UP000091820    UP000092444    UP000092443    UP000078200    UP000092460    UP000092445    UP000095301    UP000235965    UP000030742    UP000005203    UP000009046    UP000242457    UP000015103   

PRIDE

H9J2M2    Q9V460   

Pfam

PF11942   Spt5_N        + More
PF00467   KOW
PF03439   Spt5-NGN
PF04430   DUF498
PF11968   Bmt2

Interpro

IPR005824   KOW        + More
IPR039385   NGN_Euk       
IPR024945   Spt5_C_dom       
IPR036735   NGN_dom_sf       
IPR041976   KOW_Spt5_3       
IPR008991   Translation_prot_SH3-like_sf       
IPR039659   SPT5       
IPR006645   NGN_dom       
IPR041973   KOW_Spt5_1       
IPR041975   KOW_Spt5_2       
IPR041978   KOW_Spt5_5       
IPR022581   Spt5_N       
IPR017071   TF_Spt5_eukaryote       
IPR041980   KOW_Spt5_6       
IPR041977   KOW_Spt5_4       
IPR014722   Rib_L2_dom2       
IPR005100   NGN-domain       
IPR000684   RNA_pol_II_repeat_euk       
IPR036748   MTH938-like_sf       
IPR021867   Bmt2/SAMTOR       
IPR007523   NDUFAF3/AAMDC       
IPR029063   SAM-dependent_MTases       
IPR034095   NDUF3       
IPR001781   Znf_LIM       
IPR002931   Transglutaminase-like       
IPR038765   Papain-like_cys_pep_sf       

SUPFAM

SSF50104   SSF50104        + More
SSF64076   SSF64076       
SSF53335   SSF53335       
SSF54001   SSF54001       

Gene 3D

2.30.30.30     3.30.70.940     3.40.1230.10    

CDD

cd06082  KOW_Spt5_2
cd09888  NGN_Euk
cd06086  KOW_Spt5_6
cd06081  KOW_Spt5_1
cd06085  KOW_Spt5_5
cd06083  KOW_Spt5_3
cd06084  KOW_Spt5_4
cd05125  Mth938_2P1-like

ProteinModelPortal

H9J2M2   A0A2H1V6P2   A0A194QP01   A0A194QGZ7   A0A212EH19   A0A2A4K3K3   + More
K7J5S6   A0A1S4FZD5   A0A0P6IVB5   E2B534   A0A182G1W4   A0A182G3H6   A0A1I8NZL8   A0A195CEG6   A0A154P7Y4   E2A5T8   A0A0M9A996   A0A158NYZ9   A0A026W9W4   F4W9U3   A0A232EKF0   E9IQE3   A0A151WKL0   A0A195BQD0   A0A1B0EUS0   A0A182PF58   A0A084WDR7   A0A336MSR5   A0A182K2J7   U5EVY9   A0A182U6J2   A0A182KYR8   A0NE83   A0A182HQS7   A0A182XJE1   A0A182VIZ1   A0A182RUU0   A0A182M7H4   A0A182YLF7   A0A182N1S7   B4PB84   A0A232EM50   A0A0L7R2I7   A0A182QPP4   A0A182JHT9   A0A1S4J862   B3NJV0   A0A1Q3F0W5   B4MRQ6   A0A182WM74   B4LNW9   B4KME4   B0W6Z7   A0A2M3Z117   A0A182FLB7   W5J4B3   A0A0M3QVL5   Q9V460   W8C3N1   B4HPG3   A0A0J9RGS1   A0A2M4BBB8   A0A1W4VGR7   A0A1J1IGM6   Q292S1   B4GCQ3   A0A3B0J2C6   B3MGG2   A0A2M4BBF7   A0A2M4A8G2   B4J626   A0A034WJE4   A0A0L0CNG6   A0A1B0D500   D2A605   A0A1A9WAC5   A0A1B0FL32   A0A1A9YJP3   A0A1A9UYD5   A0A1B0ARK9   A0A0C9PNW0   A0A0A1WN47   A0A1A9ZRD1   A0A1I8M549   A0A2J7Q156   U4UN14   A0A088A942   E0VS63   A0A2A3E981   A0A0C9QH50   A0A023F4S8   A0A0V0G4H6   A0A069DXS5   A0A0P4VJ54   A0A224XHF9   T1I785   A0A2H8TMI3   A0A2S2Q4T6  

PDB

6GML     E-value=0,     Score=2138

KEGG

101739121    K15172 transcription elongation factor SPT5 | (RefSeq) transcription elongation factor SPT5

GO

GO:0006357   GO:0032784   GO:0005634   GO:0003746   GO:0003677   GO:0006366   GO:0005665   GO:0008168   GO:0032981   GO:0006368   GO:0003729   GO:0032044   GO:0003682   GO:0000993   GO:0045944   GO:0032785   GO:0044877   GO:0005705   GO:0000122   GO:0005703   GO:0007549   GO:0008023   GO:0032786   GO:0046982   GO:0005700   GO:0006355   GO:0005515   GO:0008017   GO:0006289   GO:0004518   GO:0006281   GO:0048384  

PANTHER

PTHR11125  

Subcellular location

Nucleus  
Chromosome  

Length:

1045

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.00125

Exp number, first 60 AAs:

0

Total prob of N-in:

0.00001

outside

1  -  1045

 

 

Pi

2.314263

Theta

5.021368

Tajima's D

-1.663896

CLR

340.015107

CSRT

0.0343482825858707

Interpretation

Possibly Positive selection