Pre Gene Modal
BGIBMGA003151
Annotation
PREDICTED:_LOW_QUALITY_PROTEIN:_DNA_topoisomerase_I?_mitochondrial_[Bombyx_mori]
Full name
DNA topoisomerase I
Alternative Name
DNA topoisomerase 1
Location in the cell
Nuclear Reliability : 4.295
Sequence
CDS
ATGAGTGTCGAAAACCCGGCCAGCGATAATGATTGTGAATCTGGAAAGGTAAATGGAAATAAAAATGAACACGTGAACGGCATGCATAACGGCTACAGTAGCTCTGACAAACACAAATCAAGCCACAAATCTTCTAGCAAAGACAAGCATCGCGACAAAGATCGCGATCACAAGGGCTCGTCCAAGCACAGCAGCTCTAGCAGAGACAAGGACAAAGACAAACACTCAAGCAGTAAAGAGCACAGCAGCTCCCATAAATCTTCCAGCAAAGATAAAGAACACAACAGTGAAAAGAGAGACAGAAGTGATAAAGATCACAGTAGCAGAGATAAAGAACGTAGTGACAAAGATAAACACCGCAGTGACAAGGACAAACATAGAGAAAAATCAAAAAGTGACAAAGATAGGCGTGATGAAAAAGACAAGAATAAAAGCAGTTCAGATAGCAAAAAGACATCATCATCATCATCATCCAGAGATAAAGAACACAAAAGCTCATCAAAGGATAAAGATCATAAATCTAAAAGTAAAGATGAAAAAGATAAAGATCGACACAGGAGTGACAAGGATAAACACCATAGGGACAAAGATAAGCACTCCAGTTCAAAAGATAAAAACAGCTCCAGCAAGAACAAGGAGAAAAGTTCAAGTGAAAGAAATGAAAAGATAAAATCTGAGTCTCAGAATGTCATAAAAGATGAACCTATGGAGGTGGATCAACCAGTCTTAAAACGTGAACAAGATGATAGTGATGAAGGATATGCTGGAGCTAACACAACTGTCTCATCTTGTGACTACTCTATGTCCCAATTTAAAGATGAGCCACTTTCTGAACTGCCTCCAGATGATGAAAGTGAAGAGGATATACCACTAACTGCACGAAAAGCTAAAAAACGCCATGTTAGTGATGGTGAAGAAGACATTCCATTACTACAAAGAAAGAAATCTAAGAAAAAAGTAAAAAAAGAAGAGACTTATGAAGATTATGATGATTATGAAGAAGATGAAAAACCTAAAAAGAAAAAGAAGAGTTCTGAAAAAACTAAATCAGTTAAGGTGAAATCTGAGCCCATAGATGATGGTATCAGTCCCAATAAACGGAAGAAAAAACAAGACACAGAAACAGAACAAGTCTGGAAATGTCCATTTTTACGTAAAGTATCATTAAGTTCTGTTTTTACCAGACACAAGCGACGATTTCCAAGAAGCAAGTGGGAAGAAGAGAAGACCGATGATGGAACAAAATGGACATTTTTGGAACATAAAGGCCCTGTGTTTGCTCCACCTTATGAACCTTTGCCTTCCAATGTCAAATTTTATTATGATGGTAAAGAGTTGAAACTATCTGAAGATGCTGAAGAAGTAGCAGGATTTTATGCACGCATGTTGGAACATGATTATACCACCAAGTCTGTTTTCAACACCAATTTTTTAACTGACTGGCGGAAAGTGATGACTCCGGAAGAAGCTAAACTTATTAAAGATTTGTCCAAATGTAACTTCAAAGAGATGCATGCATACTTTCAGAGTGTATCTGAAAAAAATAGAAATCGATCTAAAGAAGAAAAGGCTGCTTTAAAAGCCAAAAATGAAGAAATACAAAAAGAATATGGATTTTGCACAATAGATGGTCATAAAGAGAAGATAGGGAACTTCCGAATAGAACCCCCAGGGTTGTTTAGAGGTCGAGGTGAGCACCCAAAAATGGGGAAATTAAAGAGACGTGTTATGCCTGAAGATGTCTTAATCAATTGCTCAAAAGATAGTAAAATACCAAAACCACCTTCAGGACATAAATGGAGAGAAGTAAGACATGATAATACAGTTACATGGTTAGCCTCTTGGACAGAGAATGTTCAGCAGCAAGCTAAGTATGTCATGTTAAATCCCAGTTCAAAATTGAAGGGTGAAAAAGATTGGCAGAAATATGAGACTGCTAGAAAACTTCATAAGTGTATAGACAAAATTAGAGAAAATTACCGTGCTGATTGGAAGGCAAAGGAGATGCAGGTGCGCCAAAGAGCAGTGGCACTATACTTCATTGATCGATTAGCACTTAGAGCAGGTAATGAAAAAGATGATGAACAAGCTGACACTGTTGGTTGCTGTTCATTAAGAGTAGAACATATCCAGTTACACAAAGAAAAGGACGGTAAAGAATATGTAGTAGTGTTTGATTTCCTTGGTAAAGATTCAATAAGATATTACAATGAAGTGCCAGTAGAGAAACGTGTATTTAAGAATCTCGAAATATTTATTGAGAATAAAAAGGACAGTGATGATCTCTTCGACAGACTTAACACACAAACTTTAAATGAACATTTAAAAGACTTGATGCCAGGTTTGACTGCCAAGGTATTCCGTACATACAATGCTTCTATTACCCTACAGAAACAATTAGATGAGCTCACTGATGGTGATGCCTCTGTGCCTGAGAAGATTTTAGCTTACAATAGAGCAAACCGTGCAGTTGCCATCTTGTGCAACCATCAACGTGCTGTACCTAAGGGACATTCTAAGTCCATGGAGGCTTTAAAAGAAAAAATTCAAGCTAAACGGGATCAAATTGATGAGGCTGAAGAAGACCTTCAACAAGCATCTAAAGCAGCAAAGCGAGGTTCTGTTAAAGAAAAATTAGCATGTGATAAGAAGAAAAAGGCACTTGAACGTCTCAAAGACCAACTTAAAAAACTGGAACTGCAGGAAACAGACAGAGATGAAAATAAAACCATTGCCCTTGGAACATCGAAACTGAATTATTTGGATCCACGGATTTCAGTAGCATGGTGCAAGAAACATGGTGTTCCAATTGAGAAAATATATAACAAAACGCAAAGGGATAAGTTCCGATGGGCAATAGACATGGCCGGGCCAGACTATGTCTTCTAG
Protein
MSVENPASDNDCESGKVNGNKNEHVNGMHNGYSSSDKHKSSHKSSSKDKHRDKDRDHKGSSKHSSSSRDKDKDKHSSSKEHSSSHKSSSKDKEHNSEKRDRSDKDHSSRDKERSDKDKHRSDKDKHREKSKSDKDRRDEKDKNKSSSDSKKTSSSSSSRDKEHKSSSKDKDHKSKSKDEKDKDRHRSDKDKHHRDKDKHSSSKDKNSSSKNKEKSSSERNEKIKSESQNVIKDEPMEVDQPVLKREQDDSDEGYAGANTTVSSCDYSMSQFKDEPLSELPPDDESEEDIPLTARKAKKRHVSDGEEDIPLLQRKKSKKKVKKEETYEDYDDYEEDEKPKKKKKSSEKTKSVKVKSEPIDDGISPNKRKKKQDTETEQVWKCPFLRKVSLSSVFTRHKRRFPRSKWEEEKTDDGTKWTFLEHKGPVFAPPYEPLPSNVKFYYDGKELKLSEDAEEVAGFYARMLEHDYTTKSVFNTNFLTDWRKVMTPEEAKLIKDLSKCNFKEMHAYFQSVSEKNRNRSKEEKAALKAKNEEIQKEYGFCTIDGHKEKIGNFRIEPPGLFRGRGEHPKMGKLKRRVMPEDVLINCSKDSKIPKPPSGHKWREVRHDNTVTWLASWTENVQQQAKYVMLNPSSKLKGEKDWQKYETARKLHKCIDKIRENYRADWKAKEMQVRQRAVALYFIDRLALRAGNEKDDEQADTVGCCSLRVEHIQLHKEKDGKEYVVVFDFLGKDSIRYYNEVPVEKRVFKNLEIFIENKKDSDDLFDRLNTQTLNEHLKDLMPGLTAKVFRTYNASITLQKQLDELTDGDASVPEKILAYNRANRAVAILCNHQRAVPKGHSKSMEALKEKIQAKRDQIDEAEEDLQQASKAAKRGSVKEKLACDKKKKALERLKDQLKKLELQETDRDENKTIALGTSKLNYLDPRISVAWCKKHGVPIEKIYNKTQRDKFRWAIDMAGPDYVF
Summary
Description
Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at the specific target site 5'-[CT]CCTTp site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.
Catalytic Activity
ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
Similarity
Belongs to the type IB topoisomerase family.
Uniprot
EC Number
5.6.2.2
EMBL
Proteomes
Interpro
IPR013500
TopoI_cat_euk
+ More
IPR013499 TopoI_euk
IPR013030 DNA_topo_DNA_db_N_dom2
IPR014727 TopoI_cat_a/b-sub_euk
IPR014711 TopoI_cat_a-hlx-sub_euk
IPR011010 DNA_brk_join_enz
IPR018521 TopoI_AS
IPR008336 TopoI_DNA-bd_euk
IPR025834 TopoI_C_dom
IPR013034 DNA_topo_DNA_db_N_dom1
IPR036202 TopoI_DNA-bd_euk_N_sf
IPR001631 TopoI
IPR013499 TopoI_euk
IPR013030 DNA_topo_DNA_db_N_dom2
IPR014727 TopoI_cat_a/b-sub_euk
IPR014711 TopoI_cat_a-hlx-sub_euk
IPR011010 DNA_brk_join_enz
IPR018521 TopoI_AS
IPR008336 TopoI_DNA-bd_euk
IPR025834 TopoI_C_dom
IPR013034 DNA_topo_DNA_db_N_dom1
IPR036202 TopoI_DNA-bd_euk_N_sf
IPR001631 TopoI
Gene 3D
ProteinModelPortal
PDB
1NH3
E-value=0,
Score=1902
Ontologies
KEGG
GO
Topology
Length:
962
Number of predicted TMHs:
0
Exp number of AAs in TMHs:
0
Exp number, first 60 AAs:
0
Total prob of N-in:
0.00001
outside
1 - 962
Population Genetic Test Statistics
Pi
175.955289
Theta
182.124365
Tajima's D
-0.180432
CLR
191.25894
CSRT
0.318534073296335
Interpretation
Uncertain
