SGID Silkworm Genome Informatics Database
Gene
KWMTBOMO02150
Pre Gene Modal
BGIBMGA003010
Annotation
PREDICTED:_LOW_QUALITY_PROTEIN:_ATPase_WRNIP1-like_[Bombyx_mori]
Full name
ATPase WRNIP1      
Alternative Name
Werner helicase-interacting protein 1
Location in the cell
Nuclear   Reliability : 2.717
 

Sequence

CDS
ATGAGTGATTGTACAAAGACTGTGAATTGTCCTATATGTAATAGAAGTTTTGAAAATACCGAAATTGAGGAACATGTAAACAAATGTCTTTTTCTCAATTCTTGTGAAAAATCTAATTCGAAAAGACAAGGGCAACAACTATTATCTCCAAATGAAAAAAGAAAGAAAGTTGAAAAGTTAACAGCTTTGCCTCCAAACTTGAAGAGTTCACAATCACAAAAAATAAGCACAAATGAAAACATAAAAATACCTCTATCAGAGTCTTTAAGGCCAAAATCTCTGGACCAGATTGTTGGACAAAAGCAATCATTTGGCACAGATTCAATGCTCCGATCGATGCTTGAACAAAACAAGATTCCTAACATGATTTTGTGGGGTCCACCAGGCTGCGGAAAGACGTCATTAGCAAATGTTATCTCTAATATTTGTAAAAAACAAAATAATTTAAAATTTGTAAAACTATCAGCTACTACATCTGGCATCAATGATGTAAAGGAAATTGTTAAAATAGCAAAAAATGATGCACAATTTAAAAACCAGACTATTTTATTCATGGATGAAATCCACAGGTTTAACAAACTTCAACAGGACACTTTCCTGCCCCATGTTGAGAATGGTACAATAACTCTGATTGGAGCCACAACTGAAAATCCTTCTTTCAGTCTCAATAATGCTCTACTTAGTAGATGCCGGGTTGTTGTCATGGACAAACTGACCACTGAAGATGTGATGGTTATTTTAAAGAAAGCCGTTGAATACAATGAAGCAAAACTTATTAATACAAAGGAAACTGTGAACAAGAGTGATGAACCTGATCAAACCATACCCAGGTATTTAATTGAAGAAGCTTCTTTGCAATGGCTGGCAGAAATGTGTGATGGAGACGCTAGAGTCGCTTTAAGCGCCCTTGAACTGGCGCTTAATGCTCGAGATCCGGGCTCTGATGTGCACCTGAACGGCCCAGCAGTGTTAACACATGATGATATCAAAGATGGAATAAAGCGCTCGCACGTGGTATACGACAAGCACGGCGAAGAACATTACAACACGATATCTGCTATACAAAAGTGTATCCGCGCTGGAGACGATAACGCAGCGCTTTATTGGACAGCCCGAGCGATGCACGGTGGCGAAGATCCATTATATATCGCGCGACGATTTGTTAGAATGGCCTGTGAAGACATAGGCTTAGCCGATCAATCCGCATTAGTAGAAGCAGTTGCGTGTTTTCAAGGTTGCCACCAAATAGGGATGCCCGAGTGTGACGTCCTGTTGGCTCAGTGTGCTGTGAGATTAGCCCGGGCTCCGAAGAGTCGTGAAGTATACAACGCACTGAAACGGTGTCAACAATCTCTAAAAGAAACTAAAGGTCCTCTCCCACCAGTTCCATTACATTTACGGAATGCACCGACCAAGCTAATGAAGCAAATAGGGTATGGAAGTGGATACAATCTCCTGAGTAAGGAAGAATCTGGACTTACCTATTTGCCAGCAGGAGTTGAAGGTGATTATTTTCAGAAGGAATACGAAGGCGATTAA
Protein
MSDCTKTVNCPICNRSFENTEIEEHVNKCLFLNSCEKSNSKRQGQQLLSPNEKRKKVEKLTALPPNLKSSQSQKISTNENIKIPLSESLRPKSLDQIVGQKQSFGTDSMLRSMLEQNKIPNMILWGPPGCGKTSLANVISNICKKQNNLKFVKLSATTSGINDVKEIVKIAKNDAQFKNQTILFMDEIHRFNKLQQDTFLPHVENGTITLIGATTENPSFSLNNALLSRCRVVVMDKLTTEDVMVILKKAVEYNEAKLINTKETVNKSDEPDQTIPRYLIEEASLQWLAEMCDGDARVALSALELALNARDPGSDVHLNGPAVLTHDDIKDGIKRSHVVYDKHGEEHYNTISAIQKCIRAGDDNAALYWTARAMHGGEDPLYIARRFVRMACEDIGLADQSALVEAVACFQGCHQIGMPECDVLLAQCAVRLARAPKSREVYNALKRCQQSLKETKGPLPPVPLHLRNAPTKLMKQIGYGSGYNLLSKEESGLTYLPAGVEGDYFQKEYEGD

Summary

Description
Functions as a modulator of initiation or reinitiation events during DNA polymerase delta-mediated DNA synthesis. In the presence of ATP, stimulation of DNA polymerase delta-mediated DNA synthesis is decreased. Plays also a role in the innate immune defense against viruses. Stabilizes the RIG-I/DDX58 dsRNA interaction and promotes RIG-I/DDX58 'Lys-63'-linked polyubiquitination. In turn, RIG-I/DDX58 transmits the signal through mitochondrial MAVS.
Catalytic Activity
ATP + H2O = ADP + H(+) + phosphate
Subunit
Forms homooligomers (PubMed:18842586), possibly octamers. Directly interacts with POLD1, POLD2 and POLD4 (PubMed:15670210). Interacts with the N-terminal domain of WRN (By similarity). Interacts (via UBZ-type zinc finger) with monoubiquitin and polyubiquitin (PubMed:17550899, PubMed:18842586). Interacts with TRIM14 and PPP6C; these interactions positively regulate the RIG-I/DDX58 signaling pathway (PubMed:29053956).
Similarity
Belongs to the AAA ATPase family. RarA/MGS1/WRNIP1 subfamily.
Keywords
3D-structure   Acetylation   Alternative splicing   ATP-binding   Complete proteome   Cytoplasm   DNA damage   DNA replication   Hydrolase   Immunity   Innate immunity   Isopeptide bond   Metal-binding   Nucleotide-binding   Nucleus   Phosphoprotein   Reference proteome   Ubl conjugation   Zinc   Zinc-finger  
Feature
chain  ATPase WRNIP1
splice variant  In isoform 4.
EC Number
3.6.1.3
EMBL
NWSH01003201    PCG66794.1    KQ459598    KPI94695.1    AGBW02014506    OWR41553.1    + More
KQ460709    KPJ12714.1    NEVH01019395    PNF22466.1    PNF22469.1    KK852432    KDR24005.1    GBGD01001223    JAC87666.1    GBBI01004945    JAC13767.1    UFQS01000195    UFQT01000195    SSX01106.1    SSX21486.1    PNF22468.1    CH477195    EAT48398.1    GECL01001446    JAP04678.1    LBMM01000101    KMR04953.1    ACPB03008948    GL437497    EFN70363.1    GFDL01001696    JAV33349.1    GBHO01006340    JAG37264.1    GBHO01006339    JAG37265.1    GBRD01001404    JAG64417.1    GDHC01013049    JAQ05580.1    KQ980404    KYN16197.1    GDHC01010573    JAQ08056.1    GECZ01029805    JAS39964.1    ADTU01005004    KQ982330    KYQ57675.1    KQ976440    KYM86490.1    GL888175    EGI66054.1    KQ435853    KOX70673.1    KQ761797    OAD56792.1    KK107144    EZA57600.1    KQ981856    KYN34646.1    QOIP01000003    RLU24316.1    KQ977580    KYN01761.1    KQ414621    KOC67578.1    KZ288253    PBC30949.1    GEDC01027422    GEDC01005857    JAS09876.1    JAS31441.1    ATLV01013344    KE524854    KFB37715.1    GDKW01002074    JAI54521.1    JTDY01003048    KOB70248.1    ADMH02002139    ETN58327.1    GL446154    EFN88396.1    AXCM01004622    KQ434777    KZC04284.1    AXCN02001071    GFDF01003246    JAV10838.1    AJWK01025067    AJWK01025068    APCN01001809    GEZM01078986    JAV62548.1    AAAB01008986    AKHW03003201    KYO35473.1    ABQF01009080    DS469590    EDO40507.1    MUZQ01000197    OWK55548.1    AGCU01036227    AGCU01036228    AGCU01036229    AGCU01036230    AGCU01036231    KK719166    KFO61734.1    KL671847    KFW83963.1    AC157662    GG666511    EEN60701.1    MCFN01000039    OXB67408.1    KL216485    KFV69858.1    AEFK01064574    AEFK01064575    NDHI03003384    PNJ70934.1    KZ505832    PKU44347.1    ADON01092884    ADON01092885    CM001279    EHH52678.1    QUSF01000013    RLW04461.1    AB056152    AK026179    AK315471    AB209723    AK223593    AL139092    CH471087    BC018923    AF218313    AAH18923.1    BAB15383.1    BAB60709.1    BAD92960.1    BAD97313.1    CABD030042957    CABD030042958    NBAG03000244    PNI63036.1    LSYS01005643    OPJ76552.1    BC150136    AAI50137.1    AYCK01003155    AYCK01003156    QRBI01000104    RMC16071.1    CU693494    BC067729    AAH67729.1    JH431429   
Pfam
PF16193   AAA_assoc_2        + More
PF00004   AAA
PF12002   MgsA_C
PF05496   RuvB_N
PF18279   zf-WRNIP1_ubi
Interpro
IPR021886   MgsA_C        + More
IPR003959   ATPase_AAA_core       
IPR027417   P-loop_NTPase       
IPR008921   DNA_pol3_clamp-load_cplx_C       
IPR003593   AAA+_ATPase       
IPR032423   AAA_assoc_2       
IPR006642   Znf_Rad18_put       
IPR008824   RuvB-like_N       
IPR040539   Znf-WRNIP1_ubi       
SUPFAM
SSF48019   SSF48019        + More
SSF52540   SSF52540       
PDB
3PVS     E-value=3.35818e-80,     Score=761

Ontologies

Topology

Subcellular location
Nucleus   Colocalizes with WRN in granular structures in the nucleus.   With evidence from 19 publications.
Cytoplasm   Colocalizes with WRN in granular structures in the nucleus.   With evidence from 19 publications.
Length:
512
Number of predicted TMHs:
0
Exp number of AAs in TMHs:
0.01046
Exp number, first 60 AAs:
0
Total prob of N-in:
0.01233
outside
1  -  512
 
 

Population Genetic Test Statistics

Pi
166.836152
Theta
151.778204
Tajima's D
0.352733
CLR
0.633714
CSRT
0.469776511174441
Interpretation
Uncertain
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