Gene

KWMTBOMO01955

Pre Gene Modal

BGIBMGA005921

Annotation

PREDICTED:_EGF_domain-specific_O-linked_N-acetylglucosamine_transferase_[Amyelois_transitella]

Location

Bomo_Chr4(-):8685851-8697241      View in EpiBrowser

Full name

EGF domain-specific O-linked N-acetylglucosamine transferase      

Alternative Name

Extracellular O-linked N-acetylglucosamine transferase

Location in the cell

Extracellular   Reliability : 1.797 Nuclear   Reliability : 1.708

CDS

ATGACGAAACAGATTGAATTTTTATGTTTTTTATTATTTCCCATTCACACACTATCAATTAACTTTAATGAATTAAATTTGCCTCCGCAACATATTCCATTCGTTTTTAATGCATTTCCTGAAATTGCTGAAACCTGCAATTCGGAACAAAGCTGCCCTTTCAAGAGTTCGCTGGGTGCAAAATCGTGTTGGGGTTATGAACGAGACTGTGATAAAAAATCCTCGTATCATGTGCGCCCAGTGTGTCCTGGCGATCACAGAGGTTGGGTTAAAACGAAAGAAGCCCAATATGATACGTTTTACACACAAGCAGATTTTGGTTATGTTAAAGAACAAATCGACGATTTGATGGTGATGTGTGAAGCACTATACCCTGATGATACATCGCTTGAATGCTCAAAATATCTTAGGTTCTGCAGGGGAAGAAATATAATGCTGAATTTTACTGGCCTTGTGGGTCGTGGTGATAATTTGAGATATAAAACTGATATTCTCAGTGCTGGACAAATAGGTGGCTACTGTAAGTTCTATTCAGATAGACTTATGAAGGAGGCAGAACATATGAGTGCATTACAGTCATGGGGTCCAGAAATGGTGAATTTCGTCAAAACTCCTAAAAAGCCAATAGCAGATGGAATGTGTGACATTGTCATAGATAAACCAACTTACATCATGAAACTTGATGCTGCTGTTAATATGTATCATCATTTTTGTGACTTTTTCAATTTGTATGCATCGCTACACGTAAATTCCACACATCCATCAACATTTACTAGAAACAATCATATTCTAGTTTGGGAAACATTTACATATGATTCTGCATTCAAAGACGCTTTTAAGGCGTTCACTGAGAATCCGATTTGGGATTTAAAGAGGTTTAGAGGAAAAGTTGTCTGTTTTAAAAATGCAGTGTTCCCGCTTCTGCCAAGAATGATTTTTGGTCTCTACTACAACACACCTCTAATATACGGATGCGAAAGAAGTGGATTGTTTCATGCCTTTTCTAAACACATACTGCATTCTTTGAATATAAAACTGCATATGCGTACCGATGATAGAGTAAGAGTTACTCTCCTATCGAGAGGAACCACATATAGGTCAATTCTAAATGAGAAAGAAATTGTAGATGCTTTGCTCAAAGAAGATGGTTATTATGTCCAACGCGTTGTCTATGACAGGACAGTTTCATTCACAAAGCAGCTAGAAATAACTCACAATACTGATGTGTTCATTGGAATGCATGGAGCCGGTCTGACACATTTACTGTTCTTACCTGATTGGGCAGCGGTATTTGAAATATACAATTGTGAAGATCCTAACTGCTATGCTGATTTAAGTAGACTTCGAGGACTCAAATACGTAACATGGGAAGATAAATCAAAACTTGTCCAACAAGATGAGGGTCATAGTCCCGGTGGTGGTTCACATGCAAAGTTCACCAATTATTCGTTTGACGTCAAAGAATTCTTGAGGTTGGTCGCGAAGTGTGCCGAGTACGTGCGCAACAGACAAGACTTTCAGAACTTCGTTGAAGCGTCTCTTATTAAAATGCACGAAGAATTATAG

Protein

MTKQIEFLCFLLFPIHTLSINFNELNLPPQHIPFVFNAFPEIAETCNSEQSCPFKSSLGAKSCWGYERDCDKKSSYHVRPVCPGDHRGWVKTKEAQYDTFYTQADFGYVKEQIDDLMVMCEALYPDDTSLECSKYLRFCRGRNIMLNFTGLVGRGDNLRYKTDILSAGQIGGYCKFYSDRLMKEAEHMSALQSWGPEMVNFVKTPKKPIADGMCDIVIDKPTYIMKLDAAVNMYHHFCDFFNLYASLHVNSTHPSTFTRNNHILVWETFTYDSAFKDAFKAFTENPIWDLKRFRGKVVCFKNAVFPLLPRMIFGLYYNTPLIYGCERSGLFHAFSKHILHSLNIKLHMRTDDRVRVTLLSRGTTYRSILNEKEIVDALLKEDGYYVQRVVYDRTVSFTKQLEITHNTDVFIGMHGAGLTHLLFLPDWAAVFEIYNCEDPNCYADLSRLRGLKYVTWEDKSKLVQQDEGHSPGGGSHAKFTNYSFDVKEFLRLVAKCAEYVRNRQDFQNFVEASLIKMHEEL

Description

Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in extracellular proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Specifically glycosylates the Thr residue located between the fifth and sixth conserved cysteines of folded EGF-like domains. Involved in epithelial cell adhesion/interaction with the extracellular matrix by mediating glycosylation of proteins in the secretory pathway, such as Dumpy (Dp).

Catalytic Activity

L-seryl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-(N-acetyl-beta-D-glucosaminyl)-L-seryl-[protein] + H(+) + UDP
L-threonyl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonyl-[protein] + H(+) + UDP

Cofactor

a divalent metal cation

Keywords

Complete proteome   Endoplasmic reticulum   Glycoprotein   Glycosyltransferase   Reference proteome   Signal   Transferase  

Feature

chain  EGF domain-specific O-linked N-acetylglucosamine transferase

Uniprot

A0A2A4JSY7   A0A194RGU5   A0A194PRS1   A0A2W1BUZ4   A0A2H1WES0   A0A026W9G8   + More
A0A0C9RCY2   A0A067RAE9   E2ACW1   A0A151J3G5   A0A0J7KX56   A0A195F7A0   A0A195B925   H9J8S9   A0A195D087   E2BFN9   A0A2A3EMG5   A0A0N0U2M9   E9J2W8   A0A2P8ZDR8   A0A087ZN09   A0A151WYH3   K7J8M2   A0A0T6AV00   A0A232F0D6   A0A1Y1KZI8   D6WM60   A0A1W4WWK5   A0A069DV12   T1HTG8   A0A224XLQ9   A0A0P4VMH0   A0A1S3D4X9   A0A3Q0IY14   A0A158NWN3   A0A0L7R405   A0A2J7RGD5   A0A2J7RGC6   A0A1B6CH82   A0A146LVI8   A0A182PG13   A0A182RAY3   A0A182YDP3   A0A182VUI0   A0A182U7K3   A0A182X0S2   A0A1S4H0L1   Q7PVH1   A0A182M8I0   A0A182V9A8   A0A182IUV3   A0A182NU60   A0A336LWA8   A0A1Q3EXM7   A0A182QAZ9   A0A182L6F5   A0A1Q3EXU2   A0A084VWH1   W5J9Z1   A0A182F4R2   A0A182HGF9   A0A1S4FI55   A0A182K420   A0A182GAE9   A0A182G7I0   A0A1J1IGQ9   Q170B4   B0WRA4   A0A1I8QES0   A0A1I8MYS6   A0A0K8V6I6   B0X2C2   A0A1B0A1J7   A0A1B0G508   A0A1A9Y4D2   A0A1B0BG35   A0A0A1WER6   A0A0L0C8Y6   Q5NDL5   W8C3X0   A0A0M4E3K5   B4NWP0   Q5NDL4   B4GSK1   A0A0J9QUB8   B4Q838   B3N9K9   B4LUD1   E0VER0   B4KGV4   M9PBV8   Q9VQB7   A0A1W4UUC9   A0A0P4WRY1   A0A1Y1KX70   J9JUW5   B3MLI9   B4MTZ2  

EC Number

2.4.1.255

Pubmed

26354079    28756777    24508170    30249741    24845553    20798317    + More
19121390    21282665    29403074    20075255    28648823    28004739    18362917    19820115    26334808    27129103    21347285    26823975    25244985    12364791    20966253    24438588    20920257    23761445    26483478    17510324    25315136    25830018    26108605    15632085    17994087    23185243    24495485    17550304    22936249    18057021    20566863    10731132    12537568    12537572    12537573    12537574    16110336    17569856    17569867    22158438    12537569   

EMBL

NWSH01000672    PCG74896.1    KQ460398    KPJ15151.1    KQ459601    KPI93835.1    + More
KZ149895    PZC78789.1    ODYU01008149    SOQ51517.1    KK107347    QOIP01000007    EZA52321.1    RLU20673.1    GBYB01014444    JAG84211.1    KK852595    KDR20615.1    GL438586    EFN68718.1    KQ980278    KYN16979.1    LBMM01002229    KMQ95102.1    KQ981744    KYN36318.1    KQ976558    KYM80732.1    BABH01005101    BABH01005102    BABH01005103    KQ977012    KYN06333.1    GL448037    EFN85561.1    KZ288211    PBC32897.1    KQ436245    KOX67308.1    GL768054    EFZ12831.1    PYGN01000087    PSN54636.1    KQ982650    KYQ52929.1    AAZX01000971    AAZX01016287    LJIG01022744    KRT78940.1    NNAY01001362    OXU24236.1    GEZM01073428    JAV65105.1    KQ971343    EFA03354.1    GBGD01001228    JAC87661.1    ACPB03016020    GFTR01006894    JAW09532.1    GDKW01002597    JAI53998.1    ADTU01028242    KQ414661    KOC65578.1    NEVH01004401    PNF39883.1    PNF39882.1    GEDC01028744    GEDC01024760    GEDC01024546    GEDC01004390    GEDC01003895    JAS08554.1    JAS12538.1    JAS12752.1    JAS32908.1    JAS33403.1    GDHC01007933    JAQ10696.1    AAAB01008984    EAA14911.4    AXCM01002460    UFQS01000240    UFQT01000240    SSX01877.1    SSX22254.1    GFDL01014981    JAV20064.1    AXCN02000773    GFDL01015017    JAV20028.1    ATLV01017586    KE525174    KFB42315.1    ADMH02001962    ETN60228.1    APCN01004224    JXUM01050896    KQ561666    KXJ77865.1    JXUM01047550    KQ561524    KXJ78286.1    CVRI01000048    CRK98954.1    CH477477    EAT40278.1    DS232053    EDS33288.1    GDHF01017772    JAI34542.1    DS232286    EDS39145.1    CCAG010023642    JXJN01013737    GBXI01017111    JAC97180.1    JRES01000753    KNC28707.1    AJ868231    CH379061    CAI30566.1    EAL33029.1    GAMC01002597    JAC03959.1    CP012523    ALC38221.1    CM000157    EDW87382.1    AJ868232    CAI30567.1    CH479189    EDW25360.1    CM002910    KMY87667.1    CM000361    EDX03460.1    CH954177    EDV57466.1    CH940649    EDW64117.1    KRF81435.1    DS235093    EEB11866.1    CH933807    EDW11154.1    AE014134    AGB92471.1    AB675601    AY058292    GDRN01038051    GDRN01038050    JAI67269.1    GEZM01073429    JAV65101.1    ABLF02037452    CH902620    EDV31738.1    CH963852    EDW75581.1   

Proteomes

UP000218220    UP000053240    UP000053268    UP000053097    UP000279307    UP000027135    + More
UP000000311    UP000078492    UP000036403    UP000078541    UP000078540    UP000005204    UP000078542    UP000008237    UP000242457    UP000053105    UP000245037    UP000005203    UP000075809    UP000002358    UP000215335    UP000007266    UP000192223    UP000015103    UP000079169    UP000005205    UP000053825    UP000235965    UP000075885    UP000075900    UP000076408    UP000075920    UP000075902    UP000076407    UP000007062    UP000075883    UP000075903    UP000075880    UP000075884    UP000075886    UP000075882    UP000030765    UP000000673    UP000069272    UP000075840    UP000075881    UP000069940    UP000249989    UP000183832    UP000008820    UP000002320    UP000095300    UP000095301    UP000092445    UP000092444    UP000092443    UP000092460    UP000037069    UP000001819    UP000092553    UP000002282    UP000008744    UP000000304    UP000008711    UP000008792    UP000009046    UP000009192    UP000000803    UP000192221    UP000007819    UP000007801    UP000007798   

PRIDE

H9J8S9    Q9VQB7   

Pfam

PF04577   DUF563        + More
PF00078   RVT_1

Interpro

IPR007657   Glycosyltransferase_61        + More
IPR000477   RT_dom       

ProteinModelPortal

A0A2A4JSY7   A0A194RGU5   A0A194PRS1   A0A2W1BUZ4   A0A2H1WES0   A0A026W9G8   + More
A0A0C9RCY2   A0A067RAE9   E2ACW1   A0A151J3G5   A0A0J7KX56   A0A195F7A0   A0A195B925   H9J8S9   A0A195D087   E2BFN9   A0A2A3EMG5   A0A0N0U2M9   E9J2W8   A0A2P8ZDR8   A0A087ZN09   A0A151WYH3   K7J8M2   A0A0T6AV00   A0A232F0D6   A0A1Y1KZI8   D6WM60   A0A1W4WWK5   A0A069DV12   T1HTG8   A0A224XLQ9   A0A0P4VMH0   A0A1S3D4X9   A0A3Q0IY14   A0A158NWN3   A0A0L7R405   A0A2J7RGD5   A0A2J7RGC6   A0A1B6CH82   A0A146LVI8   A0A182PG13   A0A182RAY3   A0A182YDP3   A0A182VUI0   A0A182U7K3   A0A182X0S2   A0A1S4H0L1   Q7PVH1   A0A182M8I0   A0A182V9A8   A0A182IUV3   A0A182NU60   A0A336LWA8   A0A1Q3EXM7   A0A182QAZ9   A0A182L6F5   A0A1Q3EXU2   A0A084VWH1   W5J9Z1   A0A182F4R2   A0A182HGF9   A0A1S4FI55   A0A182K420   A0A182GAE9   A0A182G7I0   A0A1J1IGQ9   Q170B4   B0WRA4   A0A1I8QES0   A0A1I8MYS6   A0A0K8V6I6   B0X2C2   A0A1B0A1J7   A0A1B0G508   A0A1A9Y4D2   A0A1B0BG35   A0A0A1WER6   A0A0L0C8Y6   Q5NDL5   W8C3X0   A0A0M4E3K5   B4NWP0   Q5NDL4   B4GSK1   A0A0J9QUB8   B4Q838   B3N9K9   B4LUD1   E0VER0   B4KGV4   M9PBV8   Q9VQB7   A0A1W4UUC9   A0A0P4WRY1   A0A1Y1KX70   J9JUW5   B3MLI9   B4MTZ2  

KEGG

101744474    K18134 EGF domain-specific O-GlcNAc transferase [EC:2.4.1.255] | (RefSeq) EGF domain-specific O-link

PATHWAY

00514    Other types of O-glycan biosynthesis - Bombyx mori (domestic silkworm)

GO

GO:0016021   GO:0016757   GO:0016740   GO:0016262   GO:0005788   GO:0097363   GO:0097370   GO:0008363   GO:0006493   GO:0003676   GO:0030833   GO:0006480   GO:0008168   GO:0005737   GO:0016876   GO:0043039   GO:0004651   GO:0005634  

PANTHER

PTHR20961  

Subcellular location

Endoplasmic reticulum lumen  

SignalP

Position:   1 - 19,         Likelihood:  0.966511

 

 

Length:

521

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

1.29043

Exp number, first 60 AAs:

1.1674

Total prob of N-in:

0.06088

outside

1  -  521

 

 

Pi

195.613799

Theta

180.543879

Tajima's D

0.829446

CLR

0.35875

CSRT

0.605169741512924

Interpretation

Uncertain