Gene

KWMTBOMO01940

Pre Gene Modal

BGIBMGA005929

Annotation

PREDICTED:_protein-O-fucosyltransferase_1_isoform_X1_[Bombyx_mori]

Location

Bomo_Chr4(-):8314868-8319657      View in EpiBrowser

Full name

GDP-fucose protein O-fucosyltransferase 1      

Alternative Name

Neurotic protein
Peptide-O-fucosyltransferase 1

Location in the cell

Cytoplasmic   Reliability : 2.417 Nuclear   Reliability : 2.133

CDS

ATGGGCAGATTTGGGAACCAAGCTGATCACTTTCTGGGTGTTTTGGCTTTTAGTAAAGCAGTTAATAGAACTTTAATTCTGCCACCTTGGATTGAATACAGTATGGCCGATAGAAAAGAGAGTCTCTTTTTGCTACACTCAAAGGACAGATCAGAGTTTTATGCACCTTTGAATTATGATGCTCAGAATGAAAAGATCTTGGAACAGTGGAAAATAAGGTATCCAGTTGATAAATGGCCTGTTTTAGCCTTTACTGGTGCTCCAGCTAGTTTTCCAGTACAAAAAGAGAATGTGGATATGCAGAAATATTTAAAATGGAGTAATGATATTGAACACAGATCAAAACTTTTTATAAAAAAGAACATGAGTGGAGGAGGTTTTCTTGGAATTCATCTCAGAAATGGACAAGACTGGGTAAAAGCATGCCAGCATGTAAAAGACAGTCCAATGCTGTTTGCAGCACCACAATGCGTTGGCTACAGAAATGAACGAGGCCCATTAACAATGTCAATGTGTTTACCAAAGCCTTCTGAAATCTTAAAGCAAATAAAACGAGTAATAAAGAAACTTAGCGACATTAAATATATTTTTGTGGCGTCCGACTCTAACCATATGATAGAAGAATTAAATGCTGGCCTGCGTCGTCCAGAAATAAAGATTTTAAAATTTCAGCCATCGTCCAATCCACACTTGGATTTAGCTATTCTTGGACAAGCAAATTATTTTATTGGAAATTGTGTATCGTCATATTCAGCATTTGTCAAAAGGGAACGTGACATGAAAGGGTTACCTTCAGAATTTTGGTCATTTCCACAACGAAAGAAAAACAAACATGAAGAACTATAG

Protein

MGRFGNQADHFLGVLAFSKAVNRTLILPPWIEYSMADRKESLFLLHSKDRSEFYAPLNYDAQNEKILEQWKIRYPVDKWPVLAFTGAPASFPVQKENVDMQKYLKWSNDIEHRSKLFIKKNMSGGGFLGIHLRNGQDWVKACQHVKDSPMLFAAPQCVGYRNERGPLTMSMCLPKPSEILKQIKRVIKKLSDIKYIFVASDSNHMIEELNAGLRRPEIKILKFQPSSNPHLDLAILGQANYFIGNCVSSYSAFVKRERDMKGLPSEFWSFPQRKKNKHEEL

Description

Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue found in the consensus sequence C2-X(4,5)-[S/T]-C3 of EGF domains, where C2 and C3 are the second and third conserved cysteines. Specifically uses GDP-fucose as donor substrate and proper disulfide pairing of the substrate EGF domains is required for fucose transfer. Plays a crucial role in Notch signaling. Initial fucosylation of Notch/N by POFUT1 generates a substrate for Fringe/Fng, an acetylglucosaminyltransferase that can then extend the fucosylation on the Notch EGF repeats. This extended fucosylation is required for optimal ligand binding and canonical NOTCH signaling induced by Delta/Dl or Serrate/Ser. Also required for the transport of Notch/N to the early endosome.

Catalytic Activity

Transfers an alpha-L-fucosyl residue from GDP-beta-L-fucose to the serine hydroxy group of a protein acceptor.

Subunit

Interacts with N (via its EGF domains); the interaction is required for the endocyctic transport of N.

Similarity

Belongs to the glycosyltransferase 65 family.
Belongs to the RecA family.

Keywords

Carbohydrate metabolism   Complete proteome   Disulfide bond   Endoplasmic reticulum   Fucose metabolism   Glycoprotein   Glycosyltransferase   Notch signaling pathway   Reference proteome   Signal   Transferase  

Feature

chain  GDP-fucose protein O-fucosyltransferase 1

Uniprot

H9J8T7   Q659S0   Q00P42   A0A2H1WR48   A0A2W1BV04   A0A0L7LML0   + More
A0A194RD10   A0A212ETX2   A0A194PLH0   S4PPE1   A0A2A4K4F0   A0A1B6ILL0   A0A0J7NKK3   E1ZV69   A0A1I8MTV4   A0A1B6M642   D6WLU5   A0A2A3ENF8   B3NRC7   A0A1W4WKV2   A0A067RR47   A0A1Q3FHL3   A0A1Q3FHN2   B4JW84   B4QFD1   A0A0M8ZUH9   E0VCP3   B0X217   A0A0C9QJG1   Q659S1   A0A1Q3FH50   A0A1Q3FH11   E2BD92   K7J3H8   A0A182Y708   A8E6L6   Q9V6X7   A0A154PEI3   A0A232FEA8   A0A034WMV2   A0A1B6CCN0   B3MCZ6   A0A0K8U5B3   U4UHL0   A0A0L0BU95   A0A0M4EVV4   A0A1B6ER18   N6TUE8   B4HR13   A0A182P5P8   B4LJU0   A0A1W4VCK8   A0A0K8TKW5   A0A0L7QZ02   A0A026X319   A0A2S2PAA8   A0A182N3C1   J9KAW3   Q6EV68   Q292F1   A0A1I8NLW7   A0A293LZ27   B4GDM4   A0A182MA77   A0A0A1WL84   B4KT64   A0A1Y1MQ91   A0A182QNI9   A0A084WCI7   Q00P44   A0A2H8TGU7   Q17N26   A0A3B0JGA0   A0A336LRQ0   A0A1B0CRL3   A0A2J7RF22   A0A1A9Z1W3   B4MY74   A0A336MZV8   A0A336MRK8   A0A1J1IZC1   A0A1A9V1L9   A0A1A9W3R1   W8BMH7   V5GV70   Q7QHS7   A0A182LH93   A0A182JU40   A0A182HDQ7   A0A182HY47   A0A182WXH2   T1H0K8   A0A195FGC2   A0A151XAH6   A0A182VAD0   A0A182RLI8   A0A1B0D685   A0A182TPG4   A0A1B0F9G3   A0A158NGP3  

EC Number

2.4.1.221

Pubmed

19121390    16679357    28756777    26227816    26354079    22118469    + More
23622113    20798317    25315136    18362917    19820115    17994087    24845553    22936249    20566863    17550304    20075255    25244985    10731132    12537568    12537572    12537573    12537574    16110336    17569856    17569867    26109357    26109356    12917292    12537569    11524432    11698403    12909620    17329366    28648823    25348373    23537049    26108605    26369729    24508170    30249741    12966037    15632085    23185243    25830018    28004739    24438588    17510324    24495485    12364791    14747013    17210077    20966253    26483478    21347285   

EMBL

BABH01005075    BABH01005076    AJ831491    CAH40835.1    DQ139946    ABA29467.1    + More
ODYU01010437    SOQ55545.1    KZ149895    PZC78799.1    JTDY01000569    KOB76645.1    KQ460398    KPJ15160.1    AGBW02012530    OWR44901.1    KQ459601    KPI93843.1    GAIX01000302    JAA92258.1    NWSH01000172    PCG78788.1    GECU01019895    JAS87811.1    LBMM01003913    KMQ93000.1    GL434441    EFN74928.1    GEBQ01008577    JAT31400.1    KQ971343    EFA03399.2    KZ288204    PBC33230.1    CH954179    EDV56079.1    KK852514    KDR22209.1    GFDL01008007    JAV27038.1    GFDL01007987    JAV27058.1    CH916375    EDV98222.1    CM000362    CM002911    EDX07041.1    KMY93690.1    KQ435840    KOX71405.1    DS235060    EEB11149.1    DS232276    EDS38947.1    GBYB01000702    JAG70469.1    AJ831490    CM000158    CAH40834.1    EDW90977.1    GFDL01008186    JAV26859.1    GFDL01008185    JAV26860.1    GL447585    EFN86320.1    BT030808    AE013599    ABV82190.1    AHN56204.1    AB093572    BT021295    AY118651    KQ434874    KZC09688.1    NNAY01000337    OXU29106.1    GAKP01002066    JAC56886.1    GEDC01026119    GEDC01008701    JAS11179.1    JAS28597.1    CH902619    EDV36311.1    GDHF01030462    JAI21852.1    KB632327    ERL92517.1    JRES01001317    KNC23650.1    CP012524    ALC41820.1    GECZ01029368    JAS40401.1    APGK01051866    KB741207    ENN72900.1    CH480816    EDW47810.1    CH940648    EDW60599.2    GDAI01002629    JAI14974.1    KQ414685    KOC63801.1    KK107020    QOIP01000004    EZA62421.1    RLU23223.1    GGMR01013756    MBY26375.1    ABLF02039399    AJ781501    CAH03716.1    DQ139947    CM000071    ABA29468.1    EAL24911.1    GFWV01008372    MAA33101.1    CH479181    EDW31681.1    AXCM01001256    GBXI01015022    JAC99269.1    CH933808    EDW09584.2    GEZM01024260    JAV87822.1    AXCN02001113    ATLV01022672    KE525335    KFB47931.1    DQ139944    ABA29465.1    GFXV01001519    MBW13324.1    CH477202    EAT48119.1    OUUW01000001    SPP74380.1    UFQS01004263    UFQT01004263    SSX16292.1    SSX35612.1    AJWK01024922    NEVH01004414    PNF39432.1    CH963894    EDW77063.1    UFQT01005066    SSX35882.1    UFQS01002304    UFQT01002304    SSX13753.1    SSX33174.1    CVRI01000064    CRL05440.1    GAMC01008372    JAB98183.1    GALX01000432    JAB68034.1    DQ139945    AAAB01008816    ABA29466.1    EAA05278.4    JXUM01130315    JXUM01130316    KQ567562    KXJ69367.1    APCN01004491    CAQQ02373491    KQ981625    KYN39064.1    KQ982339    KYQ57339.1    AJVK01012008    CCAG010002095    ADTU01015163    ADTU01015164   

Proteomes

UP000005204    UP000037510    UP000053240    UP000007151    UP000053268    UP000218220    + More
UP000036403    UP000000311    UP000095301    UP000007266    UP000242457    UP000008711    UP000192223    UP000027135    UP000001070    UP000000304    UP000053105    UP000009046    UP000002320    UP000002282    UP000008237    UP000002358    UP000076408    UP000000803    UP000076502    UP000215335    UP000007801    UP000030742    UP000037069    UP000092553    UP000019118    UP000001292    UP000075885    UP000008792    UP000192221    UP000053825    UP000053097    UP000279307    UP000075884    UP000007819    UP000001819    UP000095300    UP000008744    UP000075883    UP000009192    UP000075886    UP000030765    UP000008820    UP000268350    UP000092461    UP000235965    UP000092445    UP000007798    UP000183832    UP000078200    UP000091820    UP000007062    UP000075882    UP000075881    UP000069940    UP000249989    UP000075840    UP000076407    UP000015102    UP000078541    UP000075809    UP000075903    UP000075900    UP000092462    UP000075902    UP000092444    UP000005205   

PRIDE

H9J8T7    Q659S0    Q00P42    A8E6L6    Q9V6X7   

Pfam

PF10250   O-FucT        + More
PF08423   Rad51

Interpro

IPR019378   GDP-Fuc_O-FucTrfase        + More
IPR039922   POFUT1       
IPR011941   DNA_recomb/repair_Rad51       
IPR020587   RecA_monomer-monomer_interface       
IPR013632   DNA_recomb/repair_Rad51_C       
IPR033925   Rad51_DMC1_RadA       
IPR027417   P-loop_NTPase       
IPR010995   DNA_repair_Rad51/TF_NusA_a-hlx       
IPR020588   RecA_ATP-bd       
IPR003593   AAA+_ATPase       

SUPFAM

SSF52540   SSF52540        + More
SSF47794   SSF47794       

CDD

cd01123  Rad51_DMC1_radA

ProteinModelPortal

H9J8T7   Q659S0   Q00P42   A0A2H1WR48   A0A2W1BV04   A0A0L7LML0   + More
A0A194RD10   A0A212ETX2   A0A194PLH0   S4PPE1   A0A2A4K4F0   A0A1B6ILL0   A0A0J7NKK3   E1ZV69   A0A1I8MTV4   A0A1B6M642   D6WLU5   A0A2A3ENF8   B3NRC7   A0A1W4WKV2   A0A067RR47   A0A1Q3FHL3   A0A1Q3FHN2   B4JW84   B4QFD1   A0A0M8ZUH9   E0VCP3   B0X217   A0A0C9QJG1   Q659S1   A0A1Q3FH50   A0A1Q3FH11   E2BD92   K7J3H8   A0A182Y708   A8E6L6   Q9V6X7   A0A154PEI3   A0A232FEA8   A0A034WMV2   A0A1B6CCN0   B3MCZ6   A0A0K8U5B3   U4UHL0   A0A0L0BU95   A0A0M4EVV4   A0A1B6ER18   N6TUE8   B4HR13   A0A182P5P8   B4LJU0   A0A1W4VCK8   A0A0K8TKW5   A0A0L7QZ02   A0A026X319   A0A2S2PAA8   A0A182N3C1   J9KAW3   Q6EV68   Q292F1   A0A1I8NLW7   A0A293LZ27   B4GDM4   A0A182MA77   A0A0A1WL84   B4KT64   A0A1Y1MQ91   A0A182QNI9   A0A084WCI7   Q00P44   A0A2H8TGU7   Q17N26   A0A3B0JGA0   A0A336LRQ0   A0A1B0CRL3   A0A2J7RF22   A0A1A9Z1W3   B4MY74   A0A336MZV8   A0A336MRK8   A0A1J1IZC1   A0A1A9V1L9   A0A1A9W3R1   W8BMH7   V5GV70   Q7QHS7   A0A182LH93   A0A182JU40   A0A182HDQ7   A0A182HY47   A0A182WXH2   T1H0K8   A0A195FGC2   A0A151XAH6   A0A182VAD0   A0A182RLI8   A0A1B0D685   A0A182TPG4   A0A1B0F9G3   A0A158NGP3  

PDB

5KXQ     E-value=9.52524e-42,     Score=426

KEGG

692614    K03691 peptide-O-fucosyltransferase [EC:2.4.1.221] | (RefSeq) pofut1, Fut12, O-fut1; protein-O-fucos

PATHWAY

00514    Other types of O-glycan biosynthesis - Bombyx mori (domestic silkworm)

GO

GO:0008417   GO:0006004   GO:0007219   GO:0046922   GO:0005783   GO:0005112   GO:0045747   GO:0045807   GO:0016021   GO:0016266   GO:0006493   GO:0000150   GO:0016757   GO:0005524   GO:1990426   GO:0005634   GO:0003690   GO:0000724   GO:0008094   GO:0003697  

PANTHER

PTHR21420  

Subcellular location

Endoplasmic reticulum  

Length:

281

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.66774

Exp number, first 60 AAs:

0.144

Total prob of N-in:

0.04793

outside

1  -  281

 

 

Pi

33.709744

Theta

59.529339

Tajima's D

0.105493

CLR

0.29612

CSRT

0.393830308484576

Interpretation

Uncertain