Gene

KWMTBOMO01927  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA006216

Annotation

alanine--tRNA_ligase?_cytoplasmic_[Bombyx_mori]

Location

Bomo_Chr4(+):8186974-8193960      View in EpiBrowser

Full name

Alanine--tRNA ligase, cytoplasmic      

Alternative Name

Alanyl-tRNA synthetase

Location in the cell

Cytoplasmic   Reliability : 2.09

CDS

ATGGATACTTCTATGACTGGCAATGAAATACGAAAGACCTTTATTGATTTTTTTATTAAAAAGGGTCATAAATATGTACATTCCTCGTCTACCATACCCTTAGATGATCCGACATTACTGTTTGCAAATGCAGGGATGAATCAATTTAAACCAATTTTCTTAGGTTCCGTAGATCCAAATTCTGATATGGCTCAGTATATTCGAGTAGTTAACACCCAAAAATGTATTAGAGCAGGTGGAAAACACAATGATTTGGATGACGTAGGAAAAGATGTATATCATCATACATTTTTTGAAATGATGGGAAACTGGTCATTTGGTGACTACTTTAAAAAAGAAATATGTGCTTGGGCTTGGGAACTGTTAACTGATGTTTTTAAATTATCTAGAGAGAGATTGTATGTAACATATTTTGGAGGAGACCCTTCATCTGGTCTTGAACCTGATCTCGAATGCAAAAACATTTGGTTAAACTTGGGAGTCCCAGAGGCACACATTTTGCCTGGAAGCATGAAGGATAATTTTTGGGAAATGGGTGAAACAGGTCCTTGTGGCCCGTGTTCAGAACTGCACTATGATCGAATCGGAGAACGAGAAGCTGCCCATCTTGTAAACATGGATGATCCAGACGTGTTGGAAATTTGGAATCTAGTGTTTATCCAATTCAATAGGGAAACAGATGGTTCCCTGAAATTATTACCAAAAAAACATATAGATTGTGGTTTAGGTCTTGAAAGGTTAGTATCTGTAATTCAAAACAAACGAGCTAATTATGATACTGATTTCTTCATGCCAATATTCAAAGCTATTGAAAACGCTACTGGTGTGAGACCATATTCTGGAAAAGTTGGGATTGATGATGTTGATGGAATAGACATGGCGTATCGTGTCTTGGCTGACCATGCTAGAACTCTAACAATTGCTTTATCAGATGGTGGTTGCCCTGATAACACAGGTAGAGGATACGTTCTTCGTAGAATTTTGAGAAGAGCTGTGCGATATGCGTCAGAAAAATTAAATGCCAAGCCTGGATTTTTTGGATCTTTGGTTTATACTGTAGTTGAATTACTAGGTGATGTTTTCCCAGAAATTAAAAAGGATCCAGATTCCATTGTGCATGTCATAAATGAAGAAGAAGTGCAATTTTTAAAAACCCTATTGCGAGGAAGAAATTTACTTTACAGAACAATAGAGAAGCTCAATAATTCGAAAACCTTACCTGGTGACGTGGCATGGCGTTTATATGATACTTACGGATTTCCTATTGATTTGACTCAATTAATGTGTGAAGAAAAGGGTCTTAATGTAGACATGGAAGGTTATGAAAAATCCAGAAAAGAATCTCAATTAGTTTCACAAGGAAAAACCGCAGGCCAGGAAGACTTGATAGCATTAGATGTACACGCTATTAGTCATCTACAGGATACTGGAATTCCTGCTACAGATGATTCTCCAAAATATAATTATTTACCATCATCTACTGACAAAGATGCACTCTATACATTTGCTCCGTGTACAGCAAAAATTGTCGCATTAAGGAAAAATAAGGAGTTTGTAAGTGAAATAAGTTCGGGACAGGAATGTGGTGTAATACTAGATCGCACTAGCTTTTATGCTGAACAAGGAGGCCAAATCTTCGATGAAGGTTATATGGTAAAAATTGATGACGAAACTGTTGAATTTACAGTAAAAAATGTTCAAGTAAAAGGAGGCTATGTTCTCCACGCAGGAAAGGTCGAGGGAATTTTAAAAGTAGGAGATACTCTATCCCTGCACATTGATACAGAAAGACGAAGACTTGTTATGAATAATCACACTGGAACCCATGTTCTTAATAACGTTCTAAGAAAAGTTCTTGGCAATGATTCAGATCAACGTGGATCGCTAGTAATGCCAGACAGGCTTCGATTTGATTTTACTAATAAAGGTCCCATGACAATAAAACAGATCAAAGATACGGAAAACGAAATAAAAGAAATCATTGCGAAAAATAAAACAGTATACGCAAACTACACAAGCTTGTCTGAAGCAAAAAAAATTAACGGATTGCGGGCAATGTTCGATGAACATTATCCTGATCCAGTTAGAGTCGTATCAGTAGGAGTACCCGTGGAAGACCTTATAAAAAATCCAGACGCTCCTACAGGATTCGAAACATCTGTTGAATTTTGTGGTGGATCCCACTTACACCGGACTAGCCATATTGGGGAATATGTCATTGTGAGTGAAGAAGGTATTGCAAAAGGAATCAGAAGAATTGTTGCAGTGACTGGACCAGAAGCGATAAAAGCTATCAACAAATTAAGTGTTCTTGAAAACGAGGTTAATAATGTTGCCAATTTTATAAAAGAACAAAATGAAAGTATTTCTCATAAAGAAGTATCTAAGAAAATAGTGGATCTGACGAACGAGATATCCCAAGCCCAAATATCATATTGGAAAAAAGATGAATTAAGAAATATGCTGAAGAACTTAAAAAAACAACTAGATGATAAAGAAAGAGCCGAAAAAGCTATAATTATTACTCAAGTCACAGAAAAAGCAAAAGAGCTATGTCTTGAACGCAAAGAATCAAAATATATTGTATCCGAATTAAAGGCGTTTGGAAATACCAAAGCTTTAGATGGTGCTTTAAAACAAGTGAAACAATTTTGCCCAAACTCAGCTGCGATGTTCTTTTCTGTTGATAAAGACGCTAATAAGATTTATTGCTTGGCAGCTGTGCCCAAAAGTGATGTAGAAAAGGGATTGTTAGCTTCAGAATGGGTGCAATCCGTTGTAGACATTATAGGTGGCAAAGGTGGTGGCAAAGCTGAGTCAGCTCAAGCATCTGGGAACAATCCTAATAGTTTAAATGAAGCTATCCAAATTGCAAATGAATATGCTAAGTCTAAATTAAATTAA

Protein

MDTSMTGNEIRKTFIDFFIKKGHKYVHSSSTIPLDDPTLLFANAGMNQFKPIFLGSVDPNSDMAQYIRVVNTQKCIRAGGKHNDLDDVGKDVYHHTFFEMMGNWSFGDYFKKEICAWAWELLTDVFKLSRERLYVTYFGGDPSSGLEPDLECKNIWLNLGVPEAHILPGSMKDNFWEMGETGPCGPCSELHYDRIGEREAAHLVNMDDPDVLEIWNLVFIQFNRETDGSLKLLPKKHIDCGLGLERLVSVIQNKRANYDTDFFMPIFKAIENATGVRPYSGKVGIDDVDGIDMAYRVLADHARTLTIALSDGGCPDNTGRGYVLRRILRRAVRYASEKLNAKPGFFGSLVYTVVELLGDVFPEIKKDPDSIVHVINEEEVQFLKTLLRGRNLLYRTIEKLNNSKTLPGDVAWRLYDTYGFPIDLTQLMCEEKGLNVDMEGYEKSRKESQLVSQGKTAGQEDLIALDVHAISHLQDTGIPATDDSPKYNYLPSSTDKDALYTFAPCTAKIVALRKNKEFVSEISSGQECGVILDRTSFYAEQGGQIFDEGYMVKIDDETVEFTVKNVQVKGGYVLHAGKVEGILKVGDTLSLHIDTERRRLVMNNHTGTHVLNNVLRKVLGNDSDQRGSLVMPDRLRFDFTNKGPMTIKQIKDTENEIKEIIAKNKTVYANYTSLSEAKKINGLRAMFDEHYPDPVRVVSVGVPVEDLIKNPDAPTGFETSVEFCGGSHLHRTSHIGEYVIVSEEGIAKGIRRIVAVTGPEAIKAINKLSVLENEVNNVANFIKEQNESISHKEVSKKIVDLTNEISQAQISYWKKDELRNMLKNLKKQLDDKERAEKAIIITQVTEKAKELCLERKESKYIVSELKAFGNTKALDGALKQVKQFCPNSAAMFFSVDKDANKIYCLAAVPKSDVEKGLLASEWVQSVVDIIGGKGGGKAESAQASGNNPNSLNEAIQIANEYAKSKLN

Description

Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.

Catalytic Activity

ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)

Cofactor

Zn(2+)

Subunit

Monomer.

Similarity

Belongs to the class-II aminoacyl-tRNA synthetase family.

Keywords

Aminoacyl-tRNA synthetase   ATP-binding   Complete proteome   Cytoplasm   Direct protein sequencing   Ligase   Metal-binding   Nucleotide-binding   Protein biosynthesis   Reference proteome   RNA-binding   tRNA-binding   Zinc  

Feature

chain  Alanine--tRNA ligase, cytoplasmic

Uniprot

P21894   A0A194PM98   A0A2H1WK48   A0A2A4K3R2   A0A2W1BUU3   A0A194RGW8   + More
A0A0L7LMV7   A0A212ETU8   S4NU55   A0A2P8XTW8   A0A2J7QBL6   A0A067QNU3   A0A1B6JE49   A0A1B6FEA7   A0A0T6B073   A0A1B6JZB8   D6WJA8   A0A0J7NVV1   A0A1Y1KNT1   E0VL09   A0A1W4WEE0   A0A146KMY0   E2A1R5   A0A0A9W8U6   U5EVI9   A0A182GHV3   A0A232EEH7   E2BBY0   F4WWX6   Q17LY0   A0A182IQ96   A0A0C9QUS5   A0A182Y0K3   A0A182LMA3   A0A084WU96   A0A182T0T6   A0A182QQ06   A0A182UXY4   A0A182WGF3   A0A182RHE0   A0A158NCD7   A0A0N8C3F5   A0A1S4H1T1   A0A182NZH9   A0A182HI65   A0A0P5Q5R6   A0A0P6GWU4   A0A0P5VMW4   A0A151JAY9   A0A0P6GTT9   A0A0P5QV73   A0A0N8BM13   A0A0N8CHF3   A0A0P5SMI2   A0A182XFT0   A0A0P5FWZ8   A0A0P5T717   A0A0P4YZE2   B3MP00   A0A0N8APW0   A0A0P5MY01   A0A182TP10   A0A0P5FQL1   A0A182NBM8   A0A0P5XDN4   A0A0N8C3Z1   A0A0P5Z9G7   E9FUF6   A0A0P5WP48   A0A0P5TQQ6   A0A0P5ABB0   A0A182MNC9   A0A182K4U3   A0A0P5FIT5   A0A0N8B1P1   A0A0P5YUL9   A0A0P4YA14   A0A0P5ANP7   A0A0P5ZAI0   A0A0P5WIP3   A0A0P5TTV3   A0A0P5ANP1   A0A0P5TN28   A0A0P4Z1F8   A0A0P5ZDV6   A0A0N7ZMA3   A0A0P5TIA7   A0A0P5TPM5   A0A0P6AE67   A0A0P5VU05   A0A0P5D021   A0A0P5WIH9   A0A0P5BL55   A0A0P5HVS4   A0A0P5ZGJ4   A0A0P5ZRM8   A0A0P5UFF9   A0A0P5UC08   A0A0L7RFQ7   B4HYK1  

EC Number

6.1.1.7

Pubmed

1701172    2040280    26354079    28756777    26227816    22118469    + More
23622113    29403074    24845553    18362917    19820115    28004739    20566863    26823975    20798317    25401762    26483478    28648823    21719571    17510324    25244985    20966253    24438588    21347285    12364791    17994087    18057021    21292972   

EMBL

M55993    KQ459601    KPI93859.1    ODYU01008866    SOQ52834.1    NWSH01000193    + More
PCG78534.1    KZ149895    PZC78818.1    KQ460398    KPJ15176.1    JTDY01000501    KOB76888.1    AGBW02012530    OWR44916.1    GAIX01013442    JAA79118.1    PYGN01001356    PSN35457.1    NEVH01016296    PNF25968.1    KK853129    KDR10980.1    GECU01010261    JAS97445.1    GECZ01021220    GECZ01000310    JAS48549.1    JAS69459.1    LJIG01016380    KRT80774.1    GECU01003169    JAT04538.1    KQ971343    EFA03148.1    LBMM01001317    KMQ96520.1    GEZM01077997    JAV63069.1    DS235263    EEB14065.1    GDHC01020841    JAP97787.1    GL435766    EFN72738.1    GBHO01038727    JAG04877.1    GANO01001006    JAB58865.1    JXUM01064961    KQ562323    KXJ76147.1    NNAY01005342    OXU16738.1    GL447175    EFN86804.1    GL888417    EGI61219.1    CH477210    EAT47691.1    GBYB01004387    GBYB01011004    GBYB01011007    GBYB01011008    JAG74154.1    JAG80771.1    JAG80774.1    JAG80775.1    ATLV01027023    KE525421    KFB53790.1    AXCN02000679    ADTU01011819    GDIQ01118619    JAL33107.1    AAAB01008980    APCN01005628    GDIQ01118620    JAL33106.1    GDIQ01027800    JAN66937.1    GDIP01099027    GDIP01099026    GDIP01099025    GDIP01099024    JAM04688.1    KQ979206    KYN22250.1    GDIQ01029554    JAN65183.1    GDIQ01118618    JAL33108.1    GDIQ01164555    JAK87170.1    GDIP01131423    JAL72291.1    GDIP01137923    JAL65791.1    GDIQ01254580    JAJ97144.1    GDIP01130072    JAL73642.1    GDIP01221616    JAJ01786.1    CH902620    EDV31166.1    KPU73247.1    GDIQ01254579    JAJ97145.1    GDIQ01149654    JAL02072.1    GDIQ01254578    JAJ97146.1    GDIP01085933    JAM17782.1    GDIQ01117131    JAL34595.1    GDIP01046889    JAM56826.1    GL732525    EFX88718.1    GDIP01084432    JAM19283.1    GDIP01125880    GDIP01125879    GDIP01125878    GDIP01125876    GDIP01066033    GDIP01066032    GDIP01066030    JAL77838.1    GDIP01201616    GDIP01046892    GDIP01046891    JAJ21786.1    AXCM01003715    GDIQ01254577    JAJ97147.1    GDIQ01221531    JAK30194.1    GDIP01231509    GDIP01197967    GDIP01197965    GDIP01197963    GDIP01053192    GDIP01053191    GDIP01053190    GDIP01053189    JAM50525.1    GDIP01231512    GDIP01231510    GDIP01197964    GDIP01053188    JAI91889.1    GDIP01196671    GDIP01196669    JAJ26733.1    GDIP01046890    JAM56825.1    GDIP01085934    JAM17781.1    GDIP01124624    GDIP01045396    JAL79090.1    GDIP01196670    GDIP01130030    JAJ26732.1    GDIP01125877    GDIP01066031    GDIP01051637    JAL77837.1    GDIP01231511    GDIP01199021    JAI91890.1    GDIP01123574    GDIP01087129    GDIP01045400    GDIP01045399    GDIP01045397    GDIP01045395    GDIP01043896    JAM58318.1    GDIP01231513    GDIP01196672    JAI91888.1    GDIP01125882    GDIP01125881    GDIP01124629    GDIP01064691    GDIP01064690    GDIP01064689    JAL77832.1    GDIP01124630    GDIP01124628    GDIP01124626    GDIP01043895    JAL79084.1    GDIP01124627    GDIP01123573    GDIP01087127    GDIP01045398    GDIP01043898    GDIP01043897    GDIP01043894    JAM59821.1    GDIP01168165    GDIP01125875    GDIP01096339    GDIP01095277    JAM07376.1    GDIP01164720    JAJ58682.1    GDIP01160168    GDIP01085932    JAM17783.1    GDIP01197966    GDIP01053187    JAJ25436.1    GDIQ01221532    JAK30193.1    GDIP01043899    JAM59816.1    GDIP01197968    GDIP01053193    JAM50522.1    GDIP01113786    GDIP01113785    GDIP01113784    GDIP01113783    JAL89928.1    GDIP01115222    GDIP01115221    GDIP01115220    GDIP01115219    JAL88492.1    KQ414598    KOC69822.1    CH480818    EDW52131.1   

Proteomes

UP000005204    UP000053268    UP000218220    UP000053240    UP000037510    UP000007151    + More
UP000245037    UP000235965    UP000027135    UP000007266    UP000036403    UP000009046    UP000192223    UP000000311    UP000069940    UP000249989    UP000215335    UP000008237    UP000007755    UP000008820    UP000075880    UP000076408    UP000075882    UP000030765    UP000075901    UP000075886    UP000075903    UP000075920    UP000075900    UP000005205    UP000075885    UP000075840    UP000078492    UP000076407    UP000007801    UP000075902    UP000075884    UP000000305    UP000075883    UP000075881    UP000053825    UP000001292   

PRIDE

P21894   

Pfam

PF02272   DHHA1        + More
PF07973   tRNA_SAD
PF01411   tRNA-synt_2c
PF00043   GST_C
PF02798   GST_N
PF00647   EF1G
PF00076   RRM_1

Interpro

IPR018162   Ala-tRNA-ligase_IIc_anticod-bd        + More
IPR009000   Transl_B-barrel_sf       
IPR018163   Thr/Ala-tRNA-synth_IIc_edit       
IPR018165   Ala-tRNA-synth_IIc_core       
IPR023033   Ala_tRNA_ligase_euk/bac       
IPR003156   DHHA1_dom       
IPR002318   Ala-tRNA-lgiase_IIc       
IPR018164   Ala-tRNA-synth_IIc_N       
IPR012947   tRNA_SAD       
IPR000178   TF_IF2_bacterial-like       
IPR036282   Glutathione-S-Trfase_C_sf       
IPR036249   Thioredoxin-like_sf       
IPR004046   GST_C       
IPR001662   EF1B_G_C       
IPR036433   EF1B_G_C_sf       
IPR040079   Glutathione_S-Trfase       
IPR010987   Glutathione-S-Trfase_C-like       
IPR004045   Glutathione_S-Trfase_N       
IPR012677   Nucleotide-bd_a/b_plait_sf       
IPR000504   RRM_dom       
IPR035979   RBD_domain_sf       
IPR039157   RBM18_RRM       

SUPFAM

SSF101353   SSF101353        + More
SSF55186   SSF55186       
SSF50447   SSF50447       
SSF89942   SSF89942       
SSF47616   SSF47616       
SSF52833   SSF52833       
SSF54928   SSF54928       

Gene 3D

3.30.70.1010     3.30.70.330    

CDD

cd12355  RRM_RBM18

ProteinModelPortal

P21894   A0A194PM98   A0A2H1WK48   A0A2A4K3R2   A0A2W1BUU3   A0A194RGW8   + More
A0A0L7LMV7   A0A212ETU8   S4NU55   A0A2P8XTW8   A0A2J7QBL6   A0A067QNU3   A0A1B6JE49   A0A1B6FEA7   A0A0T6B073   A0A1B6JZB8   D6WJA8   A0A0J7NVV1   A0A1Y1KNT1   E0VL09   A0A1W4WEE0   A0A146KMY0   E2A1R5   A0A0A9W8U6   U5EVI9   A0A182GHV3   A0A232EEH7   E2BBY0   F4WWX6   Q17LY0   A0A182IQ96   A0A0C9QUS5   A0A182Y0K3   A0A182LMA3   A0A084WU96   A0A182T0T6   A0A182QQ06   A0A182UXY4   A0A182WGF3   A0A182RHE0   A0A158NCD7   A0A0N8C3F5   A0A1S4H1T1   A0A182NZH9   A0A182HI65   A0A0P5Q5R6   A0A0P6GWU4   A0A0P5VMW4   A0A151JAY9   A0A0P6GTT9   A0A0P5QV73   A0A0N8BM13   A0A0N8CHF3   A0A0P5SMI2   A0A182XFT0   A0A0P5FWZ8   A0A0P5T717   A0A0P4YZE2   B3MP00   A0A0N8APW0   A0A0P5MY01   A0A182TP10   A0A0P5FQL1   A0A182NBM8   A0A0P5XDN4   A0A0N8C3Z1   A0A0P5Z9G7   E9FUF6   A0A0P5WP48   A0A0P5TQQ6   A0A0P5ABB0   A0A182MNC9   A0A182K4U3   A0A0P5FIT5   A0A0N8B1P1   A0A0P5YUL9   A0A0P4YA14   A0A0P5ANP7   A0A0P5ZAI0   A0A0P5WIP3   A0A0P5TTV3   A0A0P5ANP1   A0A0P5TN28   A0A0P4Z1F8   A0A0P5ZDV6   A0A0N7ZMA3   A0A0P5TIA7   A0A0P5TPM5   A0A0P6AE67   A0A0P5VU05   A0A0P5D021   A0A0P5WIH9   A0A0P5BL55   A0A0P5HVS4   A0A0P5ZGJ4   A0A0P5ZRM8   A0A0P5UFF9   A0A0P5UC08   A0A0L7RFQ7   B4HYK1  

PDB

5V59     E-value=0,     Score=1824

KEGG

693023    K01872 alanyl-tRNA synthetase [EC:6.1.1.7] | (RefSeq) alanyl-tRNA synthetase

PATHWAY

00970    Aminoacyl-tRNA biosynthesis - Bombyx mori (domestic silkworm)

GO

GO:0005524   GO:0000049   GO:0005737   GO:0006419   GO:0004813   GO:0008270   GO:0016021   GO:0005525   GO:0003743   GO:0003746   GO:0005739   GO:0002161   GO:0006400   GO:0016597   GO:0003676   GO:0000166   GO:0016876   GO:0043039   GO:0004651   GO:0005634   GO:0004812  

Subcellular location

Cytoplasm  

Length:

967

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.0081

Exp number, first 60 AAs:

0.00212

Total prob of N-in:

0.00041

outside

1  -  967

 

 

Pi

247.460968

Theta

198.812355

Tajima's D

1.264106

CLR

0.195064

CSRT

0.736713164341783

Interpretation

Uncertain