Gene

KWMTBOMO01730

Pre Gene Modal

BGIBMGA006131

Annotation

ICE_protein_[Bombyx_mori]

Location

Bomo_Chr4(+):2809613-2815745      View in EpiBrowser

Full name

Caspase-3       + More
Caspase      

Alternative Name

Apopain
Cysteine protease CPP32
drICE

Location in the cell

Nuclear   Reliability : 1.2

CDS

ATGGAGGAGACTTCGAACGATTTCAAAATTTTCAACGCGAACCCATCCAATTCGAGCAATAAAGAAGTCATGGATAACTCAGAAGAGACGCAACCGACAGTGAGTTTTGACCCGGAATCAGTGTATTACAATATGTCCGGAGATAAATACTTGATTATCTTCAATCATTACACCTTCAAGAAAACCAAATACTTTCAATATAAACAGCCCTCGACAAGGAACGGTACATTTGAAGATGTTAAAAGTTTAAAGGATTTATTCGAAGGTCTATCTTACAATGTTACAACTTACACCGATCTTATCTATGAAGATATTTTAAATAGAGTTGCTGAATTTTGCAAGCGCGATCATAGCAAGACCTCATGTGTTATTGTGACGATCCTGACACATGGCGATAGCGGCGAAGTATTCGCTGCCGACCAACCATACAAGATCAGCGACCTCACTAATATGATTGAGAATGCTCATCACACGCTAGTCGGCAAGCCTAAGATCTTTTTTATTCAGGCATGCCGTGGTCCCTTTATGGATGAGGGCAGAACAGTGGTGCTGGATGGTGAAAGCAGGGGCGTCTTACGCCTTCCAACGCATTCGGACTTCCTCTTCTTACATTCTTCTGTTGACGGTTATCTTTCCTACCGTGATTCAAGAGGATCGTGGCTAATCCAGACACTGTGCGAAATCATTAGAAAGAATCACAAGGAAATCGATCTACTGCATATGATCACGATGGTGAACAAAAGCATTGCATATGCGAAGAGTACGTACGTAAAGCAGATGACCGAAACTAGGTTTACTTTAACTAAGTTATTTATGTTTGAAAAGTAG

Protein

MEETSNDFKIFNANPSNSSNKEVMDNSEETQPTVSFDPESVYYNMSGDKYLIIFNHYTFKKTKYFQYKQPSTRNGTFEDVKSLKDLFEGLSYNVTTYTDLIYEDILNRVAEFCKRDHSKTSCVIVTILTHGDSGEVFAADQPYKISDLTNMIENAHHTLVGKPKIFFIQACRGPFMDEGRTVVLDGESRGVLRLPTHSDFLFLHSSVDGYLSYRDSRGSWLIQTLCEIIRKNHKEIDLLHMITMVNKSIAYAKSTYVKQMTETRFTLTKLFMFEK

Description

Important mediator of apoptosis. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond (By similarity).

Catalytic Activity

Strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequence of Asp-Xaa-Xaa-Asp-|- with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3, although Val or Ala are also accepted at this position.

Subunit

Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit.
Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 21 kDa (p21) and a 12 kDa (p12) subunit. Inactive pro-form can homodimerize. Dronc and Drice can form a stable complex (PubMed:10675329). Interacts with Diap2 (via BIR3 domain) to form a stable complex (PubMed:18166655).

Miscellaneous

The subunits are derived from the precursor sequence by a probable autocatalytic mechanism and probably by other caspases.

Similarity

Belongs to the peptidase C14A family.

Keywords

Apoptosis   Cytoplasm   Hydrolase   Protease   Thiol protease   Zymogen   3D-structure   Complete proteome   Reference proteome  

Feature

chain  Caspase-3 subunit p17

Uniprot

Q5EG73   H9J9D9   Q2HZ05   Q2HZ04   R4I3L3   G0XQG3   + More
G0XQF8   G0XQF9   A0A2H1WCN5   G0XQG2   A0A2A4K9J0   I7C0U4   F6K5S4   A0A194R219   G0XQG1   A0A2H1WCX0   A0A212F1N5   A0A194Q558   G0XQF7   A0A1E1WR75   G0XQF6   A0A087UZR2   A0A1Q3F2D4   B0WPG0   A0A182GKE2   A0A1W4W9I1   A0A0K0LBN4   A0A195CTV8   A0A1W4W9I8   A0A088A747   Q16GK6   A0A293M9N4   F4WFL7   W5JU83   A0A151WIC1   A0A1P7XV02   A0A087UZQ9   A0A1S4G240   F1NV61   K4PXX6   A0A1Y1MC92   Q17FE3   H6V6K4   Q8I955   V5G2T8   A0A0M9AAM0   A0A1L8EIG3   A0A151J4A4   B0WLP6   A0A0M4EQI3   G1NEU9   B6EEC1   P55866   A0A0L0BTI1   A0A151HYK2   B4NBM0   B4R093   O01382   A0A336MXM6   A0A3B0JG01   U3JHT6   A0A1I8M9Z6   G0XQE4   A0A1L8HLJ6   A0A182YTP8   A0A1I8P6R3   F8UU16   A0A1W4UCU4   A0A0Q3PW47   Q4KLU8   B4HZF1   G0XQE9   G1ELA2   J9JS12   B4KAN6   A9Q0J3  

EC Number

3.4.22.56
3.4.22.-

Pubmed

19121390    21740565    26354079    22118469    26483478    26073458    + More
17510324    21719571    20920257    23761445    15592404    28004739    20838655    19027856    9030578    26108605    17994087    9184225    10731132    12537572    12537569    10675329    18166655    25315136    27762356    15562597    22229544    17541728   

EMBL

AY885228    AAW79564.1    BABH01004564    BABH01004565    DQ360829    ABC94941.1    + More
DQ360830    ABC94942.1    JQ768050    AFJ04534.1    HQ328967    AEK20824.1    HQ328962    AEK20819.1    HQ328963    AEK20820.1    ODYU01007753    SOQ50797.1    HQ328966    AEK20823.1    NWSH01000036    PCG80400.1    JX040312    AFO64610.1    HM234681    AEF30499.1    KQ460855    KPJ11833.1    HQ328965    AEK20822.1    ODYU01007752    SOQ50796.1    AGBW02010853    OWR47623.1    KQ459584    KPI98545.1    HQ328961    AEK20818.1    GDQN01006847    GDQN01002154    GDQN01001576    JAT84207.1    JAT88900.1    JAT89478.1    HQ328960    AEK20817.1    KK122506    KFM82851.1    GFDL01013329    JAV21716.1    DS232023    EDS32339.1    JXUM01069817    KQ562571    KXJ75579.1    KF956388    AIX10294.1    KQ977329    KYN03569.1    CH478268    EAT33368.1    GFWV01012062    GFWV01020562    MAA36791.1    GL888120    EGI66994.1    ADMH02000120    ETN67696.1    KQ983089    KYQ47566.1    KF955542    KF955543    KZ288416    AIS23518.1    AIS23519.1    PBC26031.1    KFM82848.1    AADN05000306    AB755808    BAM62940.1    GEZM01037080    GEZM01037077    JAV82210.1    CH477271    EAT45302.1    JN794535    AFA43940.1    AF548387    AAO16241.1    GALX01004136    JAB64330.1    KQ435699    KOX80590.1    GFDG01000341    JAV18458.1    KQ980159    KYN17427.1    DS231989    EDS30611.1    CP012526    ALC47264.1    FJ266474    ACI43910.1    D89784    JRES01001482    KNC22529.1    KQ976719    KYM76754.1    CH964232    EDW81184.2    CM000364    EDX14899.1    Y12261    AE014297    AY058451    UFQT01001457    SSX30638.1    OUUW01000005    SPP81055.1    AGTO01010918    HQ328948    AEK20805.1    CM004467    OCT96911.1    JF906728    AEH76885.1    LMAW01002534    KQK80332.1    BC098991    AAH98991.1    CH480819    EDW53408.1    HQ328953    AEK20810.1    JN113041    AEK12771.1    ABLF02008613    CH933806    EDW16773.1    EF190236    ABO93468.1   

Proteomes

UP000005204    UP000218220    UP000053240    UP000007151    UP000053268    UP000054359    + More
UP000002320    UP000069940    UP000249989    UP000192223    UP000078542    UP000005203    UP000008820    UP000007755    UP000000673    UP000075809    UP000242457    UP000000539    UP000053105    UP000078492    UP000092553    UP000001645    UP000037069    UP000078540    UP000007798    UP000000304    UP000000803    UP000268350    UP000016665    UP000095301    UP000186698    UP000076420    UP000095300    UP000192221    UP000051836    UP000001292    UP000007819    UP000009192   

PRIDE

Q5EG73    H9J9D9    Q2HZ05    Q2HZ04    P55866    O01382    + More

Interpro

IPR033139   Caspase_cys_AS        + More
IPR002398   Pept_C14       
IPR002138   Pept_C14_p10       
IPR029030   Caspase-like_dom_sf       
IPR001309   Pept_C14_p20       
IPR016129   Caspase_his_AS       
IPR015917   Pept_C14A       
IPR015471   Casp7       

SUPFAM

SSF52129   SSF52129       

CDD

cd00032  CASc

ProteinModelPortal

Q5EG73   H9J9D9   Q2HZ05   Q2HZ04   R4I3L3   G0XQG3   + More
G0XQF8   G0XQF9   A0A2H1WCN5   G0XQG2   A0A2A4K9J0   I7C0U4   F6K5S4   A0A194R219   G0XQG1   A0A2H1WCX0   A0A212F1N5   A0A194Q558   G0XQF7   A0A1E1WR75   G0XQF6   A0A087UZR2   A0A1Q3F2D4   B0WPG0   A0A182GKE2   A0A1W4W9I1   A0A0K0LBN4   A0A195CTV8   A0A1W4W9I8   A0A088A747   Q16GK6   A0A293M9N4   F4WFL7   W5JU83   A0A151WIC1   A0A1P7XV02   A0A087UZQ9   A0A1S4G240   F1NV61   K4PXX6   A0A1Y1MC92   Q17FE3   H6V6K4   Q8I955   V5G2T8   A0A0M9AAM0   A0A1L8EIG3   A0A151J4A4   B0WLP6   A0A0M4EQI3   G1NEU9   B6EEC1   P55866   A0A0L0BTI1   A0A151HYK2   B4NBM0   B4R093   O01382   A0A336MXM6   A0A3B0JG01   U3JHT6   A0A1I8M9Z6   G0XQE4   A0A1L8HLJ6   A0A182YTP8   A0A1I8P6R3   F8UU16   A0A1W4UCU4   A0A0Q3PW47   Q4KLU8   B4HZF1   G0XQE9   G1ELA2   J9JS12   B4KAN6   A9Q0J3  

PDB

3SIR     E-value=8.41219e-33,     Score=349

KEGG

692735    K04397 caspase 7 [EC:3.4.22.60] | (RefSeq) ICE, BmICE; ICE protein

PATHWAY

04214    Apoptosis - fly - Bombyx mori (domestic silkworm)
04215    Apoptosis - multiple species - Bombyx mori (domestic silkworm)

GO

GO:0006915   GO:0004197   GO:0097200   GO:0009411   GO:0005737   GO:0007507   GO:0016485   GO:0097153   GO:0051402   GO:0097194   GO:0004190   GO:0010623   GO:2001269   GO:0010165   GO:0046672   GO:0006508   GO:0012501   GO:0035070   GO:0048515   GO:0045476   GO:1990525   GO:0016322   GO:0035103   GO:0046668   GO:0008234   GO:0009982   GO:0006396   GO:0030529   GO:0016020   GO:0005515   GO:0003779   GO:0016021  

PANTHER

PTHR10454   PTHR10454:SF31  

Subcellular location

Cytoplasm  

Length:

275

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.04257

Exp number, first 60 AAs:

0.01819

Total prob of N-in:

0.05286

outside

1  -  275

 

 

Pi

240.325572

Theta

199.263349

Tajima's D

0.870903

CLR

0.522711

CSRT

0.627668616569172

Interpretation

Uncertain