Gene

KWMTBOMO01534

Pre Gene Modal

BGIBMGA009097

Annotation

PREDICTED:_N6-adenosine-methyltransferase_subunit_METTL14_[Amyelois_transitella]

Location

Bomo_Chr3(-):13053247-13062378      View in EpiBrowser

Full name

N6-adenosine-methyltransferase non-catalytic subunit      

Alternative Name

Karyogamy protein 4
Methyltransferase-like protein 14

Location in the cell

Extracellular   Reliability : 3.891

CDS

ATGAGTGAGAAATTAAGAGAACTACGCGAGAGATCTCAAAAACGAAAAAAGCTTCTAGCACAGACATTAGGTGTCTCGAGTGTGAGCGAATTAAGACATGCGCTGGGTTCAGCTCTAGACGTACCTGTTAAAAAACAAGCTGTGTCTGGAAGCGGAGGTGAAGGAGATAAACCCACCAGTAAGGACCAACCGGATGAACTTGTATACACCGACTCCTCTACATTTTTGAAGGGCACGCAGTCATCAAATCCTCACAATGACTATTGTCAGCACTTTGTTGACACAGGTCAGAGGCCACAAAACTATATCAGAGATGTTGGGCTCGCTGACAGGTTTGAGGAATATCCGAAATTACGTGAACTCATTAAACTGAAAGACGATTTAATTGCGGAAACTGCAACACCACCTATGTATTTGAAATGTGATCTCAAGACTTTTGATCTAAAAGACATGGCTAACAAATTCGATGTAATTCTCGTGGAACCTCCTCTGGGCACTGGATGGAGATGGGAAGATGTCTTAGCTCTTGAGCTCCAGCAGATAGCACAGCCAAGATCTTTTGTATTCCTATGGTGTGGAAGCTCTGAAGGTTTAGATATGGGCAGGGAATGCCTAAAGAAGTGGGGCTTTCGTCGTTGCGAAGACATCTGCTGGATCAAAACGAACATAAAGAATCCGGGGCATTCGAAAAACCTCGAGCACAACGCGGTCTTCCAGCGAACCAAGGAGCATTGTCTGATGGGCATCAAAGGCACTGTCCGGAGGTCGGTCGACGGAGACTTCATACACGCTAACGTGGATATCGACCTCATCATATCTGAAGACCCGGAGTTCGGCAGTACAGAGAAGCCTATTGAGATATTTCACATCATGGAACATTTCTGTTTGGGTCGAAGAAGGGTACACCTGTTCGGCAAGGACTCCACGATCCGTCCGGGCTGGGTGACGATCGGCCACGAGCTCACCAACTCCAACTTCAACGCGGAGCTGTACTCGTCGTACTTCGCCGAGGGCCGCGAGAGCACCGGCTGCACCGACAGGATCGAGGCCCTCAGGCCCAAGAGCCCCCCCAACACCAGCAAGGGCACCCGGGCCAGGGGCAGGGGCGGGTTTAGGGGCAGGGGGAGGGGCAGAGGGGCTATCTAA

Protein

MSEKLRELRERSQKRKKLLAQTLGVSSVSELRHALGSALDVPVKKQAVSGSGGEGDKPTSKDQPDELVYTDSSTFLKGTQSSNPHNDYCQHFVDTGQRPQNYIRDVGLADRFEEYPKLRELIKLKDDLIAETATPPMYLKCDLKTFDLKDMANKFDVILVEPPLGTGWRWEDVLALELQQIAQPRSFVFLWCGSSEGLDMGRECLKKWGFRRCEDICWIKTNIKNPGHSKNLEHNAVFQRTKEHCLMGIKGTVRRSVDGDFIHANVDIDLIISEDPEFGSTEKPIEIFHIMEHFCLGRRRVHLFGKDSTIRPGWVTIGHELTNSNFNAELYSSYFAEGRESTGCTDRIEALRPKSPPNTSKGTRARGRGGFRGRGRGRGAI

Description

Non-catalytic component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of mRNAs, a modification that plays a role in the efficiency of mRNA splicing and is required for sex determination (PubMed:27919077, PubMed:28675155). In the heterodimer formed with Ime4/Mettl3, Mettl14 constitutes the RNA-binding scaffold that recognizes the substrate rather than the catalytic core (By similarity). Required for sex determination and dosage compensation via Sxl alternative splicing: m6A methylation acts as a key regulator of Sxl pre-mRNA and promotes female-specific alternative splicing of Sxl, which determines female physiognomy (PubMed:27919077, PubMed:28675155). M6A methylation is also required for neuronal functions (PubMed:27919077).

Subunit

Component of the WMM complex, a N6-methyltransferase complex composed of a catalytic subcomplex, named MAC, and of an associated subcomplex, named MACOM (PubMed:29535189, PubMed:28675155, PubMed:29555755). The MAC subcomplex is composed of Ime4/Mettl3 and Mettl14 (PubMed:29535189, PubMed:28675155, PubMed:29555755). The MACOM subcomplex is composed of fl(2)d, Flacc/Xio, Hakai, vir, and, in some cases of nito (PubMed:29535189, PubMed:29555755).

Similarity

Belongs to the MT-A70-like family.

Keywords

Complete proteome   Differentiation   mRNA processing   mRNA splicing   Nucleus   Reference proteome   RNA-binding   Sexual differentiation  

Feature

chain  N6-adenosine-methyltransferase non-catalytic subunit

Uniprot

H9JHU9   A0A2A4JE14   A0A2H1WD35   S4PPN1   A0A212EQ94   A0A194Q349   + More
A0A1B6JJ16   A0A067R4L5   A0A1B6FC80   A0A2J7QGL2   A0A1B6MUY7   A0A0V0GAS6   T1IF26   A0A1W4WIV3   A0A069DT12   A0A1B6CCX0   A0A0T6BBE1   A0A1Y1KBE4   A0A0L7R541   A0A2A3EGX1   A0A088AW80   E0VZ87   A0A084WGV9   A0A182PMQ2   A0A1S3DF93   U5EWJ3   A0A023ERB4   F4WST8   A0A158NH17   A0A182VIQ9   A0A182TU14   A0A182WU37   A0A182L1H8   Q7Q3Z3   A0A026W1X5   A0A182HUP9   A0A182JK76   A0A182JQW9   A0A232FEV4   U4UDX9   A0A151J2F9   D6WV92   A0A195FN99   A0A151XDB5   A0A195B7B5   A0A310SBJ6   N6U3Q7   K7J3F6   A0A224XSA1   A0A1Q3F5M1   B0XBL4   A0A195D2S2   A0A182QI67   E9IMQ8   A0A0J7KXN8   A0A336MSY0   A0A336MRK6   A0A182NUC8   A0A182RZU5   A0A182M6Z5   A0A182W742   W8AGV8   W5J7V1   A0A2M4AM74   A0A182F4U6   A0A2M4BQL7   A0A182Y076   E2AJE3   T1PHX2   A0A0L0CDK7   A0A1I8Q120   A0A0A9XRN9   A0A1B0CZE0   A0A0K8UQI4   B4HYI2   A0A1W4UVI0   A0A1B0GCJ0   A0A0P4W8M7   Q9VLP7   D3TN32   A0A1B0BL96   A0A034WVG8   B4Q6Q0   A0A1B3PD81   A0A087UWN5   J9JVM4   B3N737   B4NWV8   A0A2H8TTH1   A0A2S2P3H8   A0A0N8AB70   Q29MN2   A0A3B0K1H3   A0A0P5MBK1   A0A2S2PYI1   A0A1L8DHF6   T1INW0   B4G902   A0A1A9Y3J6   B4MTJ3  

Pubmed

19121390    23622113    22118469    26354079    24845553    26334808    + More
28004739    20566863    24438588    24945155    26483478    21719571    21347285    20966253    12364791    14747013    17210077    24508170    30249741    28648823    23537049    18362917    19820115    20075255    21282665    24495485    20920257    23761445    25244985    20798317    25315136    26108605    25401762    26823975    17994087    10731132    12537572    12537569    27919077    27919081    29535189    28675155    29555755    20353571    25348373    22936249    17550304    15632085   

EMBL

BABH01029525    BABH01029526    BABH01029527    NWSH01001919    PCG69680.1    ODYU01007843    + More
SOQ50973.1    GAIX01000147    JAA92413.1    AGBW02013278    OWR43662.1    KQ459562    KPI99768.1    GECU01008539    JAS99167.1    KK852699    KDR18181.1    GECZ01021988    JAS47781.1    NEVH01014361    PNF27717.1    GEBQ01000247    JAT39730.1    GECL01000940    JAP05184.1    ACPB03007378    GBGD01001799    JAC87090.1    GEDC01031023    GEDC01026047    JAS06275.1    JAS11251.1    LJIG01002312    KRT84628.1    GEZM01093889    JAV56087.1    KQ414654    KOC65944.1    KZ288256    PBC30532.1    DS235851    EEB18693.1    ATLV01023763    KE525346    KFB49453.1    GANO01001389    JAB58482.1    JXUM01071699    GAPW01001895    KQ562679    JAC11703.1    KXJ75339.1    GL888327    EGI62745.1    ADTU01015435    AAAB01008964    EAA12386.3    KK107487    QOIP01000006    EZA50070.1    RLU22167.1    APCN01002471    NNAY01000310    OXU29304.1    KB632099    ERL88796.1    KQ980447    KYN16013.1    KQ971363    EFA09090.2    KQ981387    KYN41968.1    KQ982294    KYQ58347.1    KQ976574    KYM80089.1    KQ761783    OAD57010.1    APGK01050753    KB741176    ENN73207.1    GFTR01005555    JAW10871.1    GFDL01012277    JAV22768.1    DS232641    EDS44326.1    KQ976973    KYN06664.1    AXCN02000765    GL764255    EFZ18091.1    LBMM01002075    KMQ95307.1    UFQS01002368    UFQT01002368    SSX13897.1    SSX33316.1    UFQS01001522    UFQT01001522    SSX11339.1    SSX30907.1    AXCM01002772    GAMC01021668    JAB84887.1    ADMH02002111    ETN58884.1    GGFK01008558    MBW41879.1    GGFJ01006236    MBW55377.1    GL439972    EFN66464.1    KA648412    AFP63041.1    JRES01000536    KNC30331.1    GBHO01020192    GBHO01020191    GBRD01011887    GDHC01017266    JAG23412.1    JAG23413.1    JAG53937.1    JAQ01363.1    AJVK01009544    GDHF01031244    GDHF01023566    JAI21070.1    JAI28748.1    CH480818    EDW52112.1    CCAG010021443    GDRN01081061    JAI62083.1    AE014134    AY069354    EZ422834    ADD19110.1    JXJN01016271    GAKP01000323    JAC58629.1    CM000361    CM002910    EDX04209.1    KMY89035.1    KU659872    AOG17671.1    KK122029    KFM81774.1    ABLF02012760    ABLF02012761    CH954177    EDV59333.1    CM000157    EDW88488.1    GFXV01005435    MBW17240.1    GGMR01011361    MBY23980.1    GDIP01164577    LRGB01000626    JAJ58825.1    KZS17486.1    CH379060    EAL33661.2    OUUW01000004    SPP79809.1    GDIQ01169672    JAK82053.1    GGMS01001374    MBY70577.1    GFDF01008310    JAV05774.1    JH431216    CH479180    EDW28832.1    CH963852    EDW75432.1   

Proteomes

UP000005204    UP000218220    UP000007151    UP000053268    UP000027135    UP000235965    + More
UP000015103    UP000192223    UP000053825    UP000242457    UP000005203    UP000009046    UP000030765    UP000075885    UP000079169    UP000069940    UP000249989    UP000007755    UP000005205    UP000075903    UP000075902    UP000076407    UP000075882    UP000007062    UP000053097    UP000279307    UP000075840    UP000075880    UP000075881    UP000215335    UP000030742    UP000078492    UP000007266    UP000078541    UP000075809    UP000078540    UP000019118    UP000002358    UP000002320    UP000078542    UP000075886    UP000036403    UP000075884    UP000075900    UP000075883    UP000075920    UP000000673    UP000069272    UP000076408    UP000000311    UP000095301    UP000037069    UP000095300    UP000092462    UP000001292    UP000192221    UP000092444    UP000000803    UP000092460    UP000000304    UP000054359    UP000007819    UP000008711    UP000002282    UP000076858    UP000001819    UP000268350    UP000008744    UP000092443    UP000007798   

PRIDE

H9JHU9    Q9VLP7   

Pfam

PF05063   MT-A70

Interpro

IPR029063   SAM-dependent_MTases        + More
IPR007757   MT-A70-like       

SUPFAM

SSF53335   SSF53335       

ProteinModelPortal

H9JHU9   A0A2A4JE14   A0A2H1WD35   S4PPN1   A0A212EQ94   A0A194Q349   + More
A0A1B6JJ16   A0A067R4L5   A0A1B6FC80   A0A2J7QGL2   A0A1B6MUY7   A0A0V0GAS6   T1IF26   A0A1W4WIV3   A0A069DT12   A0A1B6CCX0   A0A0T6BBE1   A0A1Y1KBE4   A0A0L7R541   A0A2A3EGX1   A0A088AW80   E0VZ87   A0A084WGV9   A0A182PMQ2   A0A1S3DF93   U5EWJ3   A0A023ERB4   F4WST8   A0A158NH17   A0A182VIQ9   A0A182TU14   A0A182WU37   A0A182L1H8   Q7Q3Z3   A0A026W1X5   A0A182HUP9   A0A182JK76   A0A182JQW9   A0A232FEV4   U4UDX9   A0A151J2F9   D6WV92   A0A195FN99   A0A151XDB5   A0A195B7B5   A0A310SBJ6   N6U3Q7   K7J3F6   A0A224XSA1   A0A1Q3F5M1   B0XBL4   A0A195D2S2   A0A182QI67   E9IMQ8   A0A0J7KXN8   A0A336MSY0   A0A336MRK6   A0A182NUC8   A0A182RZU5   A0A182M6Z5   A0A182W742   W8AGV8   W5J7V1   A0A2M4AM74   A0A182F4U6   A0A2M4BQL7   A0A182Y076   E2AJE3   T1PHX2   A0A0L0CDK7   A0A1I8Q120   A0A0A9XRN9   A0A1B0CZE0   A0A0K8UQI4   B4HYI2   A0A1W4UVI0   A0A1B0GCJ0   A0A0P4W8M7   Q9VLP7   D3TN32   A0A1B0BL96   A0A034WVG8   B4Q6Q0   A0A1B3PD81   A0A087UWN5   J9JVM4   B3N737   B4NWV8   A0A2H8TTH1   A0A2S2P3H8   A0A0N8AB70   Q29MN2   A0A3B0K1H3   A0A0P5MBK1   A0A2S2PYI1   A0A1L8DHF6   T1INW0   B4G902   A0A1A9Y3J6   B4MTJ3  

PDB

5TEY     E-value=1.33303e-136,     Score=1246

KEGG

101738928    K05925 mRNA m6A methyltransferase [EC:2.1.1.348] | (RefSeq) methyltransferase-like protein 14 homolo

GO

GO:0008168   GO:0016422   GO:0005634   GO:0030237   GO:0005737   GO:0000381   GO:0036396   GO:0001734   GO:0008380   GO:0007548   GO:0003723   GO:0030154   GO:0080009   GO:0006397   GO:0001510   GO:0016021   GO:0006139   GO:0005515   GO:0004725   GO:0016311   GO:0016791   GO:0006418   GO:0006334   GO:0003779  

Subcellular location

Nucleus  

Length:

381

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.00117

Exp number, first 60 AAs:

0.00117

Total prob of N-in:

0.01248

outside

1  -  381

 

 

Pi

368.548902

Theta

176.825143

Tajima's D

3.417066

CLR

0.369437

CSRT

0.992700364981751

Interpretation

Uncertain