SGID Silkworm Genome Informatics Database
Gene
KWMTBOMO01422
Pre Gene Modal
BGIBMGA008958
Annotation
serine_protease_inhibitor_[Danaus_plexippus]
Full name
Reversion-inducing cysteine-rich protein with Kazal motifs      
Location in the cell
Extracellular   Reliability : 2.816
 

Sequence

CDS
ATGCGCTGGTTAATTGGAGCTTCTGAAAATATGAGGCACGGCAAGAGTGGCGGAGGCCGGGCCCGGTGGGAATCCAGGCTGCTCGTTGCTGCTATTGCGTTGGCTTTTGTAAATGCTCAAGATATATCAATATGTTGTGATAAAGCGGACAAAGCTGAAGGAACATGTCGCAGCGTATGTGAGAAGATGTCCCAGCTCAATATGACGACAGACAGATCTGTTATAGAGCAAAGCACGCCGAATATTTACAAATTTTGCGGTCATCACTTGACAGAGTTCTGGATTTGCATGAATCAAACAATACAAGAGGTGGTGTCGGGAACAGGGTGGTGGGGTCAAGTGTGCTGTCAATTGAGCCGATCATCGGAATGTCGGGCTGCGTGCGCTAGTGCTCTGGACGCGAAAGCCCTCACCGAATCCTGTCGTCGATCTGACGAAATAGCCATGTTCGACTGCATGGATAAACAACAGGAGGCTCAGTGGTGCTGTTCACAGACGCAGTCTGTGTCGTGTCACGAAGCGTGCCAGAAAGTGTTGAGGAGGCTGGGACACTCGCGCATCGATGGCGACGCGAAGGAAGCAGCAGTTGATGCTTGCGAACAATCACCAGAGCTACTGCACTGCCTCAGAGACTTGACGTCTTCTACGGTAGTTGGAGACACGTCTAAATATCTTCCATGCTGCCATGAATCCGATTCTCAAAGCTGTCGAAGTACTTGTGAGTCTGTGTTGCGTCGGACCAGTGACATAGAAGAGATCGTAGAGGCATTGGGCCAAGACTGTGGAGCTCCCGCCATACATAATGTACTGTGGCAATGCTTTTTGAGGAAAGATGCACCGACGGACACCAAGGATCTGATTCCGAGGGACGTTGCGAATTTGCATTGTTGTTTGAAGGCGAAATCAGCAACGTGCAAGGAACTGTGTTTCCACACATTCAACAAGGGGTCGCAGGAAAAATGGGATATGTTTGACCTACAGTGCTTAGATAATTCACAGGAGACAATGTTGTCGCAATGCCTCGAGGATGTGCGTACTCCATGCTCGCTGGGATGCTCCAGTCTCACTTACTGCAGTCAACTGAACGATCGTCACACGACCATGTTCAGGTCTTGTAACGTGCGCGCCGACTTGGACGCACACTTGTCGATGGCCGAACAAGAAGGCCGCAGAACGCTGCAAGTGGCCGGTATGCATTTACCAGTGAAAAACTCAAGCGAGTGCTCGACAGATCTGTGGAAAAGCGTTGCGTGCGTTTTACACGTTAAGCCTTGCACCACCAAGGGTTTAAGTAGTCTTTTGTGCGAAGAAGATTGCGTGCAGATGTTATCGTCGTGTGTGGATTGGGCACAGTTCAAGTTGCCGTACACAGCTAATACGTTGTGCGCCCGGATTGTGCCGCAGTCCAACACGACCAACTGTGTGTCCTTGCAACAGTACATGAAGCCCAGTACGAGCAGGTTGGCGGCGCCCGCGTCCCGTCCGTGCGCCGGCGCCCCGTGCAGCCGCGCACAGCTGTGCCGCGCTGACCGGAACTGTGCGCTCGCCGTCGCCTCTACTGCCGCTGCCTGCCGCAGCTACCACTGCCTTGACGGGTGCCCGCTCGGAGAAGGAAATTCTTACGTGGTGCCCATCGGGGCGTGGGTGAGGGTGTTCATGTCGGATTTATCTCACGGCCTGTGCTTCAAGATCTGCAAGTGCGAAGGGAAGGGCCTCACGAAATGTCAGCCGCTCCCCTGCTTTAGCATGCAGCACTGCTTCGTCAACAATAAACTTATCCTACACGGTGAGAAGTTTTACATGGAGTGCAACGCGTGCGTGTGCGTGTGGGGCGAGCGCGTGTGTGCGCGACGTGCGTGCGGCGCGAGGCGCGGCTCGCGACTGACGCGTCTCCCCTGCAACTGCCCGCCGCACCACCTCCCTGCGCACACCAACCACGTGCTCTACCCCAACGCCTGTCTCGCCAGGTGTGTGGGAGCGACGGACGCTGAGATAGAGTTCGGCGTCCAGTCTCCATGCGCCGGAGTCACGTGTCCGAGACGTCACGTGTGCGTGCCCTCCTCCAACGTATGCCTGTCTCGTCTCCAAACATCCTGCCCTCAACACGTTTGTGTGAATACAACAGACTGCAATTCGCAAGCCGCTATGCCGGTGTGTGACACTGATGGCAGAACACATGCGAACCCCTGCCATCTTGTGATGAGCGGCAGGAAGATGGGCTATTGGGGACCGTGTCTTCGAGGATGTTCCTCGTCCGTTGCCGTATGCGGTGCGAATGGCGTAACGTACGGTTCCGAATGTGCCGCTTGGAGCGAGTACGTGAGCGTTGATTACGTCGGCCCTTGTCTCGCGGTCGGATATATATCGGACCAAATGGAGCCGAAATGTACCTTCGACAGAATAGTCTGTCCAGCGTTGAAGAAGGACGACTGTCAAGGGTTCACGGCGCCGGGCGCTTGCTGCCCGAGATGTGGTGGGGCATTGCGGATCTTGTATTCGAAGAAACAAATCGACCGAGCCCTTTACGGTACAAACATATCCGCGACCGTTGTTAATCTCCACAATGTAGTGCAAGGCTTAGAGAGACACATCACCATAGCCGAATGCGCTCTGAGAGGCTATTTGACCATCGAAACGGAAATATTCGTTACGATAGAATCACTAATTGAAAATCCAACAGATTTACAATTGAAAATGTGTATCCTTGAGGCCGAGAAATTGGCTGATATGATTAATTCGGAACATCCTTTGATAGCAGTTGATTTGAGTCTGAGTGCCCTCTCATACGCGTTAGTAGTTCACAAACACCCAACTAAAGGTTCGTCTACTATAGCTCTATCCGTTTTCGTATTAGTCTCTTCATATTTTAGTGTTTACGTATTAAGATAG
Protein
MRWLIGASENMRHGKSGGGRARWESRLLVAAIALAFVNAQDISICCDKADKAEGTCRSVCEKMSQLNMTTDRSVIEQSTPNIYKFCGHHLTEFWICMNQTIQEVVSGTGWWGQVCCQLSRSSECRAACASALDAKALTESCRRSDEIAMFDCMDKQQEAQWCCSQTQSVSCHEACQKVLRRLGHSRIDGDAKEAAVDACEQSPELLHCLRDLTSSTVVGDTSKYLPCCHESDSQSCRSTCESVLRRTSDIEEIVEALGQDCGAPAIHNVLWQCFLRKDAPTDTKDLIPRDVANLHCCLKAKSATCKELCFHTFNKGSQEKWDMFDLQCLDNSQETMLSQCLEDVRTPCSLGCSSLTYCSQLNDRHTTMFRSCNVRADLDAHLSMAEQEGRRTLQVAGMHLPVKNSSECSTDLWKSVACVLHVKPCTTKGLSSLLCEEDCVQMLSSCVDWAQFKLPYTANTLCARIVPQSNTTNCVSLQQYMKPSTSRLAAPASRPCAGAPCSRAQLCRADRNCALAVASTAAACRSYHCLDGCPLGEGNSYVVPIGAWVRVFMSDLSHGLCFKICKCEGKGLTKCQPLPCFSMQHCFVNNKLILHGEKFYMECNACVCVWGERVCARRACGARRGSRLTRLPCNCPPHHLPAHTNHVLYPNACLARCVGATDAEIEFGVQSPCAGVTCPRRHVCVPSSNVCLSRLQTSCPQHVCVNTTDCNSQAAMPVCDTDGRTHANPCHLVMSGRKMGYWGPCLRGCSSSVAVCGANGVTYGSECAAWSEYVSVDYVGPCLAVGYISDQMEPKCTFDRIVCPALKKDDCQGFTAPGACCPRCGGALRILYSKKQIDRALYGTNISATVVNLHNVVQGLERHITIAECALRGYLTIETEIFVTIESLIENPTDLQLKMCILEAEKLADMINSEHPLIAVDLSLSALSYALVVHKHPTKGSSTIALSVFVLVSSYFSVYVLR

Summary

Description
Functions together with ADGRA2 to enable brain endothelial cells to selectively respond to Wnt7 signals (WNT7A or WNT7B) (By similarity). Plays a key role in Wnt7-specific responses: required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation (By similarity). Acts as a Wnt7-specific coactivator of canonical Wnt signaling by decoding Wnt ligands: acts by interacting specifically with the disordered linker region of Wnt7, thereby conferring ligand selectivity for Wnt7. ADGRA2 is then required to deliver RECK-bound Wnt7 to frizzled by assembling a higher-order RECK-ADGRA2-Fzd-LRP5-LRP6 complex. Also acts as a serine protease inhibitor (By similarity).
Subunit
Interacts (via knot repeats) with WNT7A (via disordered linker region); the interaction is direct (By similarity). Interacts (via knot repeats) with WNT7B (via disordered linker region); the interaction is direct (By similarity). Interacts with ADGRA2; the interaction is direct (By similarity).
Similarity
Belongs to the RECK family.
Keywords
Cell membrane   Complete proteome   Disulfide bond   Glycoprotein   GPI-anchor   Lipoprotein   Membrane   Protease inhibitor   Reference proteome   Repeat   Serine protease inhibitor   Signal   Wnt signaling pathway  
Feature
chain  Reversion-inducing cysteine-rich protein with Kazal motifs
EMBL
BABH01012509    BABH01012510    ODYU01008542    SOQ52240.1    AGBW02014011    OWR42403.1    + More
KQ459562    KPI99870.1    JTDY01000294    KOB77848.1    KQ459989    KPJ18670.1    FX985320    BAX07333.1    GECU01035412    JAS72294.1    NEVH01017557    PNF23882.1    KK852562    KDR21309.1    GECZ01022773    JAS46996.1    GEZM01057464    JAV72324.1    AJWK01018423    AJWK01018424    AJWK01018425    AJWK01018426    AJWK01018427    AJWK01018428    GEBQ01025656    JAT14321.1    KQ971363    KYB25704.1    LJIG01002556    KRT84384.1    APGK01057138    APGK01057139    KB741277    ENN71304.1    GBXI01014635    GBXI01001098    JAC99656.1    JAD13194.1    JXUM01113753    KQ565722    KXJ70743.1    CH477388    EAT42029.1    GAKP01019733    JAC39219.1    GDHF01026188    GDHF01019963    GDHF01014535    JAI26126.1    JAI32351.1    JAI37779.1    GDHF01004512    JAI47802.1    ADTU01007680    ADTU01007681    GAMC01011577    JAB94978.1    QOIP01000001    RLU27233.1    JXUM01122738    KQ566637    KXJ69958.1    KQ764883    OAD54276.1    KK107087    EZA59966.1    GGFL01000585    MBW64763.1    AAAB01008848    EAA07049.5    JXJN01011146    JXJN01011147    APCN01003560    APCN01003561    DS232720    EDS45114.1    ATLV01021421    ATLV01021422    KE525318    KFB46496.1    ABLF02037564    ABLF02037571    ABLF02037582    KQ976467    KYM84104.1    GL435237    EFN73694.1    KQ980713    KYN14336.1    KQ977935    KYM98628.1    GFXV01000394    MBW12199.1    LBMM01002314    KMQ94995.1    AXCM01001764    GL887532    EGI70924.1    JRES01001278    KNC24010.1    GL449698    EFN82131.1    ADMH02000370    ETN66801.1    KQ982649    KYQ53025.1    GBBI01003809    JAC14903.1    AXCN02002037    KQ414615    KOC68649.1    CH933809    EDW19729.1    KRG06786.1    KRG06787.1    KQ981905    KYN33504.1    CVRI01000066    CRL06213.1    GL763494    EFZ19589.1    GBYB01009531    JAG79298.1    GAPW01000223    JAC13375.1    KL887774    KGL75230.1    AAQR03161306    AAQR03161307    AAQR03161308    AAQR03161309    AAQR03161310    AAQR03161311    AAQR03161312    AAQR03161313    AAQR03161314    AAQR03161315    CP012525    ALC44627.1    AAGW02033522    AAGW02033523    AAGW02033524    AAPE02045407    AAPE02045408    JQ904540    AFM99480.1    KL512302    KFO86810.1    AGTP01043633    LSYS01006073    OPJ76128.1    KK504715    KFP60942.1    KK719167    KFO61757.1    ABQF01023895    ABQF01023896    ABQF01023897    ABQF01023898    ABQF01023899    ABQF01023900    KK690009    KFQ16190.1    AC133832    CH473962    EDL98778.1    KK833995    KFP78656.1    AEMK02000005    DQIR01113855    HDA69331.1    AADN04000072   
Pfam
PF07648   Kazal_2        + More
PF05375   Pacifastin_I
PF00050   Kazal_1
Interpro
IPR039016   RECK        + More
IPR002350   Kazal_dom       
IPR036058   Kazal_dom_sf       
IPR008037   Pacifastin_dom       
SUPFAM
SSF100895   SSF100895       

Ontologies

Topology

Subcellular location
Cell membrane  
SignalP
Position:   1 - 39,         Likelihood:  0.770399
 
 
Length:
962
Number of predicted TMHs:
0
Exp number of AAs in TMHs:
16.03027
Exp number, first 60 AAs:
0.09556
Total prob of N-in:
0.00504
outside
1  -  962
 
 

Population Genetic Test Statistics

Pi
258.996505
Theta
174.353864
Tajima's D
1.772434
CLR
0.235321
CSRT
0.845957702114894
Interpretation
Uncertain
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