Gene

KWMTBOMO01354

Pre Gene Modal

BGIBMGA008895

Annotation

PREDICTED:_polypeptide_N-acetylgalactosaminyltransferase_3-like_[Bombyx_mori]

Location

Bomo_Chr3(+):8480397-8486916      View in EpiBrowser

Full name

Polypeptide N-acetylgalactosaminyltransferase       + More
Polypeptide N-acetylgalactosaminyltransferase 3      

Alternative Name

Protein-UDP acetylgalactosaminyltransferase
Protein-UDP acetylgalactosaminyltransferase 3
UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3

Location in the cell

PlasmaMembrane   Reliability : 1.737

CDS

ATGCTTCGGACCGAGTTTGGTGCTTTGTTATGTCGAGGACGTAAGAAAAGGATTGTGACATTGATACTAATATTGATATTTTTAATAAACGCTATGTTTTATGTTAGTTTTGAATTGTATAATACGGTTATGAGAAAAAATAAGAAGCTCTGGTATAACAGATTGAAGTATGATAAGAACTCATATGTTGATGAAAGTGGTATGAGAGTTATCGTCGGACATTATGTTGGTGGTATAGGAGGAGCGAATCTATCAGAAGAAATAATGCACACTAATAATTATTCACCAGTACCTGGAGCCGGAGAGGGTGGTAGACCTGTGCAGTTGTCTCCACGAGAGCTCATTACTGCTAGAGAGCTCTATACATTACATTCTTATAATATACTTGTTAGTGATAGGATAGCTATTAATAGAAGTCTTCCTGATATGCGAAGTGATAGTTGTAGAAGCGTCGTGTATGATACGGAAGAACTTCCGACTGCTAGTGTTATAATAGTCTTCCATAATGAGGCGTGGTCTACGTTAATGAGGACCATCATGTCAGTTCTGATGAGGTCCCCACAGCTTTTACTAAAGCAGATAATTCTTGTTGACGATGCGAGTGACAGGAGATATCTAAGTGAGGAATTGGATAACGCAGTAGCTAATTTAGACAAAGTGCAAATACTGAGGAGTTCAAACAGGAATGGTTTGGTTGGAGCCAGGTTGATGGGCGCCAGGGTTGCAACTGGAGATGTGTTAGTCTTTTTGGACGCACATTGTGAGGTTACAAAAGGATGGCTCGAGCCCCTATTGGATAGAGCAGGTAATGATGACGTTTTCATTTGTCCCCACATAGATCTATTATCAGAGGACACTTTAGCCTATACTAAAAGTATAGACGCCCACTGGGGTGCGTTCAGTTGGCGCTTACATTTCCGTTGGCTCACTCCGAGTGGAGATGTAATGTTAAAGAAGATTAGGAATCCGTCAAAACCGTATCCCACGCCGGCGATGGCGGGAGGATTGTTCGCAGTCAGAAAAAGTTTGTTCTGGAGATTGGGCGGTTATGATTCGGGTATGCTGATATGGGGCGCTGAGAACTTGGAGTTGTCGTGGAGGGCATGGCAGTGCGGTGCAAGGGTTGAAATAACGCCGTGCTCACGAGTCGGACATATATTCAGGAGACATAGTCCGTACAAATATCCAGGGGGTGTATCTAAAGTGTTGAACTCGAATTTGGCTCGCGCTGCCACAGTGTGGATGGACGAATGGGCAGATTTCTTCTTCAAATTCAACCCGCCAATTTCCGCGATACGCGATCAACAAAACGTTTACGATAGAGTGGAATTACGTAAGAATTTACACTGCAAAAGTTTCGAATGGTATTTAGAGAATGTGTGGCCGCAACACTTTTTTCCGACAACCAAGAGATGGTTTGGGCGAATCAAAAATGAAAAAGGAGCTTGTTTGGGTATTACTGGAGGAACACCAGGACTTGGAGCTCCGGCTTTGGGTCTGCAATGCGGTACTGACTTAGACCTTGATAGACTACTTGTCTATACTCCGGAAGGCAAGATAATGGCGGACGAGGGGCTGTGTTTGCAGCAAGGAAACGGCCGAGCCGTGTGGAAAAGTTGTAGTGACAATAAAAAGCAAATTTGGGTGCAAGATGGTGCTAGATTAAAAACTATAAGTGGTTCTTGTCTTACGTTGCTAATCTCCGAGAAAAATAGTGATGGTATTGATGAAGCACTAACGTCTAAGCGATGCGTTGAAGACGATAGTCAAATATGGCATTTTGAACGTGTGCCTTGGCGCTGA

Protein

MLRTEFGALLCRGRKKRIVTLILILIFLINAMFYVSFELYNTVMRKNKKLWYNRLKYDKNSYVDESGMRVIVGHYVGGIGGANLSEEIMHTNNYSPVPGAGEGGRPVQLSPRELITARELYTLHSYNILVSDRIAINRSLPDMRSDSCRSVVYDTEELPTASVIIVFHNEAWSTLMRTIMSVLMRSPQLLLKQIILVDDASDRRYLSEELDNAVANLDKVQILRSSNRNGLVGARLMGARVATGDVLVFLDAHCEVTKGWLEPLLDRAGNDDVFICPHIDLLSEDTLAYTKSIDAHWGAFSWRLHFRWLTPSGDVMLKKIRNPSKPYPTPAMAGGLFAVRKSLFWRLGGYDSGMLIWGAENLELSWRAWQCGARVEITPCSRVGHIFRRHSPYKYPGGVSKVLNSNLARAATVWMDEWADFFFKFNPPISAIRDQQNVYDRVELRKNLHCKSFEWYLENVWPQHFFPTTKRWFGRIKNEKGACLGITGGTPGLGAPALGLQCGTDLDLDRLLVYTPEGKIMADEGLCLQQGNGRAVWKSCSDNKKQIWVQDGARLKTISGSCLTLLISEKNSDGIDEALTSKRCVEDDSQIWHFERVPWR

Description

Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. It can both act as a peptide transferase that transfers GalNAc onto unmodified peptide substrates, and as a glycopeptide transferase that requires the prior addition of a GalNAc on a peptide before adding additional GalNAc moieties. Prefers EA2 as substrate. Has weak activity toward Muc5AC-3, -13 and -3/13 substrates. Plays a critical role in the regulation of integrin-mediated cell adhesion during wing development by influencing, via glycosylation, the secretion and localization of the integrin ligand Tig to the basal cell layer interface.

Catalytic Activity

L-seryl-[protein] + UDP-N-acetyl-alpha-D-galactosamine = 3-O-[N-acetyl-alpha-D-galactosaminyl]-L-seryl-[protein] + H(+) + UDP
L-threonyl-[protein] + UDP-N-acetyl-alpha-D-galactosamine = 3-O-[N-acetyl-alpha-D-galactosaminyl]-L-threonyl-[protein] + H(+) + UDP

Cofactor

Mn(2+)

Similarity

Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.

Keywords

Complete proteome   Disulfide bond   Glycoprotein   Glycosyltransferase   Golgi apparatus   Lectin   Manganese   Membrane   Metal-binding   Reference proteome   Signal-anchor   Transferase   Transmembrane   Transmembrane helix  

Feature

chain  Polypeptide N-acetylgalactosaminyltransferase

Uniprot

H9JH98   A0A212F596   A0A194PUF8   A0A2W1BV78   A0A2H1VGA6   A0A194RMI0   + More
A0A1Y1MNF4   A0A067R7V4   D6WWQ3   A0A2J7RBU6   J9K3I4   A0A146LW32   A0A2S2P4H4   A0A0K8TBB6   A0A0C9QJW7   E0VX75   A0A0T6B172   A0A1W4XAW7   A0A026W442   A0A2H8TH72   A0A151J9S3   A0A158P1T7   A0A2S2R5Q9   A0A1B6CFV1   U4TZY3   A0A2A3EKJ3   A0A151XAL9   A0A0L7R5F8   A0A1B6EPV9   F4W6Q8   K7J884   A0A088APX4   A0A195CLS3   A0A195EQH4   A0A154P779   A0A195AWN0   A0A162Q9R2   A0A2P6KG00   A0A0P5YH87   A0A0P6FTD9   A0A0K2VIY1   A0A1S3DJY0   E2A7T4   T1HSC3   E2B2L3   A0A226EH74   E9H1K7   A0A0P4XQ53   A0A0P5KKA9   A0A0P5L8K0   A0A0M8ZQ52   A0A0P5MES9   A0A0A9YEM7   A0A183UCT4   T1L225   A0A0P5G6V0   A0A0N4VJP9   A0A0N8ASS8   A0A3B0JPI1   A0A1I7VTE4   A0A336KLY3   A0A182E8W0   T1JAK0   B4GCL2   Q292V0   A0A0P6HJM5   A0A0L0CCV9   B4P1D5   Q9Y117   A0A0M4EJ61  

EC Number

2.4.1.-
2.4.1.41

Pubmed

19121390    22118469    26354079    28756777    28004739    24845553    + More
18362917    19820115    26823975    20566863    24508170    30249741    21347285    23537049    21719571    20075255    20798317    21292972    25401762    25217238    22919073    17994087    15632085    26108605    17550304    10731132    12537572    12537569    12829714    16251381    18835818    20371600   

EMBL

BABH01012709    AGBW02010217    OWR48907.1    KQ459591    KPI96957.1    KZ149894    + More
PZC78879.1    ODYU01002421    SOQ39878.1    KQ459989    KPJ18732.1    GEZM01026154    JAV87204.1    KK852639    KDR19622.1    KQ971361    EFA08098.1    NEVH01005890    PNF38302.1    ABLF02017875    ABLF02017876    ABLF02017877    ABLF02017878    ABLF02017879    ABLF02045082    ABLF02060713    GDHC01013711    GDHC01013593    GDHC01008069    JAQ04918.1    JAQ05036.1    JAQ10560.1    GGMR01011701    MBY24320.1    GBRD01003120    JAG62701.1    GBYB01000902    JAG70669.1    DS235829    EEB17981.1    LJIG01016278    KRT81081.1    KK107436    QOIP01000002    EZA50822.1    RLU26044.1    GFXV01001247    MBW13052.1    KQ979361    KYN21820.1    ADTU01006849    GGMS01015867    MBY85070.1    GEDC01025083    GEDC01023436    GEDC01009517    GEDC01004538    JAS12215.1    JAS13862.1    JAS27781.1    JAS32760.1    KB631813    ERL86372.1    KZ288232    PBC31551.1    KQ982335    KYQ57423.1    KQ414654    KOC66059.1    GECZ01029810    JAS39959.1    GL887707    EGI70134.1    KQ977595    KYN01650.1    KQ982021    KYN30463.1    KQ434831    KZC07795.1    KQ976731    KYM76374.1    LRGB01000372    KZS19491.1    MWRG01013029    PRD25231.1    GDIP01057739    JAM45976.1    GDIQ01045819    JAN48918.1    HACA01032570    CDW49931.1    GL437384    EFN70514.1    ACPB03021813    GL445159    EFN90069.1    LNIX01000004    OXA56071.1    GL732583    EFX74488.1    GDIP01239683    JAI83718.1    GDIQ01184360    JAK67365.1    GDIQ01174606    JAK77119.1    KQ435915    KOX68825.1    GDIQ01156712    JAK95013.1    GBHO01034668    GBHO01034665    GBHO01013518    JAG08936.1    JAG08939.1    JAG30086.1    UYWY01019470    VDM37613.1    CAEY01000924    GDIQ01246436    JAK05289.1    UXUI01010807    VDD95644.1    GDIQ01246435    JAK05290.1    OUUW01000001    SPP73088.1    JH712080    EFO27036.2    UFQS01000613    UFQT01000613    SSX05397.1    SSX25758.1    UYRW01001012    VDK73133.1    JH432001    CH479181    EDW31460.1    CM000071    EAL24761.1    GDIQ01019131    JAN75606.1    JRES01000678    KNC29299.1    CM000157    EDW89137.1    AE013599    AF145655    CP012524    ALC41255.1   

Proteomes

UP000005204    UP000007151    UP000053268    UP000053240    UP000027135    UP000007266    + More
UP000235965    UP000007819    UP000009046    UP000192223    UP000053097    UP000279307    UP000078492    UP000005205    UP000030742    UP000242457    UP000075809    UP000053825    UP000007755    UP000002358    UP000005203    UP000078542    UP000078541    UP000076502    UP000078540    UP000076858    UP000079169    UP000000311    UP000015103    UP000008237    UP000198287    UP000000305    UP000053105    UP000050794    UP000267007    UP000015104    UP000038041    UP000274131    UP000268350    UP000095285    UP000077448    UP000271087    UP000008744    UP000001819    UP000037069    UP000002282    UP000000803    UP000092553   

PRIDE

H9JH98    Q9Y117   

Pfam

PF00652   Ricin_B_lectin        + More
PF00535   Glycos_transf_2
PF02709   Glyco_transf_7C

Interpro

IPR000772   Ricin_B_lectin        + More
IPR035992   Ricin_B-like_lectins       
IPR029044   Nucleotide-diphossugar_trans       
IPR001173   Glyco_trans_2-like       
IPR027791   Galactosyl_T_C       

SUPFAM

SSF50370   SSF50370        + More
SSF53448   SSF53448       

Gene 3D

3.90.550.10    

CDD

cd00161  RICIN

ProteinModelPortal

H9JH98   A0A212F596   A0A194PUF8   A0A2W1BV78   A0A2H1VGA6   A0A194RMI0   + More
A0A1Y1MNF4   A0A067R7V4   D6WWQ3   A0A2J7RBU6   J9K3I4   A0A146LW32   A0A2S2P4H4   A0A0K8TBB6   A0A0C9QJW7   E0VX75   A0A0T6B172   A0A1W4XAW7   A0A026W442   A0A2H8TH72   A0A151J9S3   A0A158P1T7   A0A2S2R5Q9   A0A1B6CFV1   U4TZY3   A0A2A3EKJ3   A0A151XAL9   A0A0L7R5F8   A0A1B6EPV9   F4W6Q8   K7J884   A0A088APX4   A0A195CLS3   A0A195EQH4   A0A154P779   A0A195AWN0   A0A162Q9R2   A0A2P6KG00   A0A0P5YH87   A0A0P6FTD9   A0A0K2VIY1   A0A1S3DJY0   E2A7T4   T1HSC3   E2B2L3   A0A226EH74   E9H1K7   A0A0P4XQ53   A0A0P5KKA9   A0A0P5L8K0   A0A0M8ZQ52   A0A0P5MES9   A0A0A9YEM7   A0A183UCT4   T1L225   A0A0P5G6V0   A0A0N4VJP9   A0A0N8ASS8   A0A3B0JPI1   A0A1I7VTE4   A0A336KLY3   A0A182E8W0   T1JAK0   B4GCL2   Q292V0   A0A0P6HJM5   A0A0L0CCV9   B4P1D5   Q9Y117   A0A0M4EJ61  

PDB

1XHB     E-value=6.94638e-99,     Score=923

KEGG

101735893    K00710 polypeptide N-acetylgalactosaminyltransferase [EC:2.4.1.41] | (RefSeq) polypeptide N-acetylga

PATHWAY

00512    Mucin type O-glycan biosynthesis - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)

GO

GO:0005794   GO:0016021   GO:0030246   GO:0016757   GO:0006486   GO:0000139   GO:0016740   GO:0031589   GO:0070278   GO:0033628   GO:0016266   GO:0004653   GO:0009312   GO:0046872   GO:0006493   GO:0005795   GO:0008376  

Subcellular location

Golgi apparatus membrane  

Length:

600

Number of predicted TMHs:

1

Exp number of AAs in TMHs:

21.5396699999999

Exp number, first 60 AAs:

21.46449

Total prob of N-in:

0.99607

POSSIBLE N-term signal

sequence

inside

1  -  20

TMhelix

21  -  43

outside

44  -  600

 

 

Pi

230.679969

Theta

174.29066

Tajima's D

0.847638

CLR

0.709687

CSRT

0.614619269036548

Interpretation

Uncertain