Gene

KWMTBOMO01314

Annotation

tRNA-splicing_endonuclease_subunit_Sen2_[Papilio_xuthus]

Location

Bomo_Chr3(-):7919342-7921734      View in EpiBrowser

Full name

tRNA-splicing endonuclease subunit Sen2      

Location in the cell

Nuclear   Reliability : 1.684 PlasmaMembrane   Reliability : 1.159

CDS

ATGTCTATTGAATCGTTGCGGTTTTCATTAGATAACTCGATACAAATTATATTTACTGGGTACTACAATGGTTACAATGTTGAAGTGAGGTCACCTGAAGAAATTACTCTTTTGTATCACATGGGCTGTTTTGGGAAGGGATCTGCCTCGAGGTCACGACCCGCACTTGTAAACAGTGATCGTTATCCAGAAATAATAAGGAAAAGGCAATATTTACAAAGGAACAATTGGTACAAAAAATTTGGTGATAGTGGGTGTACACTGGTTTCAAGTACTCTTTTGAAAGAATTAGATGAGTTGACGACCAAAATTGTCAGTGATGGTGAAAAGTTAAAGAAAAAAGATATAATAGATCTAGTATCCAGCGATGAAGAGTTTAGTCATGATGATGTAGACACAGAGAATGCTGTCAATCAATCTTTTTCTAGTAGCGAAAAAGATGAAATTACAGTAGTAATACCTAATAGTGATTCTGAAGAAGAAAATTATTTTGCGGCACTAAAGCCACAATGCTGTGTAAATAAAATTAAAATGCAAGAAAAATTAATATTAACTCCAGAAGAAGCATTCTTCTTAGCTTATGGACTTGGAAGTTTACAAGTAGTATACAATAATAATATTTTAGATTTAGAACAGTGTTGGACATTACTTTGTTCTAATGATAGACATTTTGTGGACAGATATGTAGTTTATCATTATTTTAGATCAAAAGGATATGTTGTGAAACCAGGGATCAAGTTTGGAGGAGACTTTTTACTGTACAAAGATGGCCCTGGCATAAGACATGCAGACTATATCATTGTTATAAAGTCTGATACAAAACAAGACGACTTAATTACTATTCTCGGTCATGTAAGAGTAGCCACAACTACTGTTAAGGAAATACTGAGGTATTATTGA

Protein

MSIESLRFSLDNSIQIIFTGYYNGYNVEVRSPEEITLLYHMGCFGKGSASRSRPALVNSDRYPEIIRKRQYLQRNNWYKKFGDSGCTLVSSTLLKELDELTTKIVSDGEKLKKKDIIDLVSSDEEFSHDDVDTENAVNQSFSSSEKDEITVVIPNSDSEEENYFAALKPQCCVNKIKMQEKLILTPEEAFFLAYGLGSLQVVYNNNILDLEQCWTLLCSNDRHFVDRYVVYHYFRSKGYVVKPGIKFGGDFLLYKDGPGIRHADYIIVIKSDTKQDDLITILGHVRVATTTVKEILRYY

Description

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body.

Catalytic Activity

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

Similarity

Belongs to the tRNA-intron endonuclease family.

Uniprot

A0A2A4J933   A0A1E1WDU7   A0A2H1VZ68   A0A194Q0Q6   A0A194RMX1   I4DKI7   + More
S4P8D4   A0A212EQ63   A0A2W1B3V2   A0A0L7L3P3   A0A0M9A9L0   A0A0V0G5X8   A0A023F762   A0A069DSU4   A0A158NER2   A0A195AVX4   A0A195FLS4   F4WRN4   A0A0J7L6I7   A0A0A9WLE0   E2A157   A0A195DZI4   A0A151XIL0   A0A195CSS9   A0A1B6K3U0   A0A1B6K494   A0A1B6FBI2   A0A1B6EUH5   A0A0T6B2I9   A0A1B6CGZ8   A0A1B6CQ19   R4G610   A0A0P4VNP3   N6TBY2   A0A3Q0IQD0   U4URJ8   A0A293M5Q4   A0A2R5LL66   A0A1W4Z8I0   A0A1W4ZH24   A0A151PJK8   A0A087U425   A0A0P7V5I5   R7YM19   A0A2R7VXN4   A0A2I0TNC2   A0A1B6DAB1   A0A2Z6Q608   A0A369RW89   A0A340XRE3   D6WVU6   A0A2R9B439   A0A2I1GT05   A0A2U3VLH8   A0A372QZV8   A0A015JUM4   A0A2P4P0E6   A0A015LVY7   A0A2H5R8G1  

EC Number

4.6.1.16

Pubmed

26354079    22651552    23622113    22118469    28756777    26227816    + More
25474469    26334808    21347285    21719571    25401762    20798317    27129103    23537049    22293439    30042472    18362917    19820115    22722832    24277808    29355972   

EMBL

NWSH01002535    PCG68044.1    GDQN01005906    JAT85148.1    ODYU01005035    SOQ45524.1    + More
KQ459591    KPI96990.1    KQ459989    KPJ18764.1    AK401805    BAM18427.1    GAIX01005991    JAA86569.1    AGBW02013301    OWR43628.1    KZ150370    PZC71149.1    JTDY01003163    KOB70035.1    KQ435709    KOX80010.1    GECL01002652    JAP03472.1    GBBI01001635    JAC17077.1    GBGD01001726    JAC87163.1    ADTU01013612    KQ976736    KYM76119.1    KQ981490    KYN41281.1    GL888292    EGI63041.1    LBMM01000509    KMQ98183.1    GBHO01034338    GBHO01034337    GBHO01034333    GBHO01024131    GBRD01016281    GBRD01016280    GBRD01016278    GBRD01006488    JAG09266.1    JAG09267.1    JAG09271.1    JAG19473.1    JAG49545.1    GL435707    EFN72824.1    KQ979999    KYN18310.1    KQ982080    KYQ60242.1    KQ977381    KYN03174.1    GECU01001598    JAT06109.1    GECU01001436    JAT06271.1    GECZ01022192    JAS47577.1    GECZ01028218    JAS41551.1    LJIG01016176    KRT81387.1    GEDC01024564    JAS12734.1    GEDC01021691    JAS15607.1    GAHY01000045    JAA77465.1    GDKW01002030    JAI54565.1    APGK01036017    KB740932    ENN77809.1    KB632408    ERL95138.1    GFWV01011503    MAA36232.1    GGLE01006148    MBY10274.1    AKHW03000123    KYO49024.1    KK118056    KFM72114.1    JARO02004499    KPP68408.1    JH767561    EON62957.1    KK854121    PTY11661.1    KZ508362    PKU35298.1    GEDC01014712    JAS22586.1    BEXD01000226    GBB85517.1    NOWV01000170    RDD38571.1    KQ971358    EFA08240.1    AJFE02092284    AJFE02092285    AJFE02092286    LLXI01000782    PKY49771.1    QKKE01000270    RGB32459.1    JEMT01026602    EXX58799.1    AUPC02000491    POG58857.1    EXX58798.1    BDIQ01000020    GBC14382.1   

Proteomes

UP000218220    UP000053268    UP000053240    UP000007151    UP000037510    UP000053105    + More
UP000005205    UP000078540    UP000078541    UP000007755    UP000036403    UP000000311    UP000078492    UP000075809    UP000078542    UP000019118    UP000079169    UP000030742    UP000192224    UP000050525    UP000054359    UP000034805    UP000016924    UP000247702    UP000253843    UP000265300    UP000007266    UP000240080    UP000234323    UP000245340    UP000263633    UP000022910    UP000018888    UP000236242   

Pfam

PF01974   tRNA_int_endo        + More
PF02778   tRNA_int_endo_N

Interpro

IPR006677   tRNA_intron_Endonuc_cat-like        + More
IPR006678   tRNA_intron_Endonuc_N       
IPR016589   tRNA_splic_SEN2       
IPR011856   tRNA_endonuc-like_dom_sf       
IPR036167   tRNA_intron_Endo_cat-like_sf       
IPR006676   tRNA_splic       

SUPFAM

SSF53032   SSF53032       

Gene 3D

3.40.1350.10    

ProteinModelPortal

A0A2A4J933   A0A1E1WDU7   A0A2H1VZ68   A0A194Q0Q6   A0A194RMX1   I4DKI7   + More
S4P8D4   A0A212EQ63   A0A2W1B3V2   A0A0L7L3P3   A0A0M9A9L0   A0A0V0G5X8   A0A023F762   A0A069DSU4   A0A158NER2   A0A195AVX4   A0A195FLS4   F4WRN4   A0A0J7L6I7   A0A0A9WLE0   E2A157   A0A195DZI4   A0A151XIL0   A0A195CSS9   A0A1B6K3U0   A0A1B6K494   A0A1B6FBI2   A0A1B6EUH5   A0A0T6B2I9   A0A1B6CGZ8   A0A1B6CQ19   R4G610   A0A0P4VNP3   N6TBY2   A0A3Q0IQD0   U4URJ8   A0A293M5Q4   A0A2R5LL66   A0A1W4Z8I0   A0A1W4ZH24   A0A151PJK8   A0A087U425   A0A0P7V5I5   R7YM19   A0A2R7VXN4   A0A2I0TNC2   A0A1B6DAB1   A0A2Z6Q608   A0A369RW89   A0A340XRE3   D6WVU6   A0A2R9B439   A0A2I1GT05   A0A2U3VLH8   A0A372QZV8   A0A015JUM4   A0A2P4P0E6   A0A015LVY7   A0A2H5R8G1  

PDB

2CV8     E-value=1.74092e-14,     Score=191

KEGG

101738015    K15322 tRNA-splicing endonuclease subunit Sen2 [EC:4.6.1.16] | (RefSeq) tRNA-splicing endonuclease s

GO

GO:0000214   GO:0016829   GO:0006388   GO:0003676   GO:0000213   GO:0008033   GO:0000379   GO:0004518   GO:0016042   GO:0005634   GO:0006334   GO:0003779   GO:0016021  

PANTHER

PTHR21227  

Subcellular location

Nucleus  

Length:

299

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.03703

Exp number, first 60 AAs:

0.00152

Total prob of N-in:

0.00765

outside

1  -  299

 

 

Pi

269.551786

Theta

190.223148

Tajima's D

1.342111

CLR

0.143333

CSRT

0.750612469376531

Interpretation

Uncertain