Gene

KWMTBOMO01310

Pre Gene Modal

BGIBMGA008877

Annotation

PREDICTED:_uncharacterized_protein_LOC106720929_[Papilio_machaon]

Location

Bomo_Chr3(+):7904655-7906963      View in EpiBrowser

Full name

Leucine-rich repeat and guanylate kinase domain-containing protein      

Location in the cell

Mitochondrial   Reliability : 1.468

CDS

ATGCTATTAGAACTAGCCGTGTACATGCGGCAGTTGCGGCGATTAATAGTATTGGATCTACGTGCCAACCCAGTTTGTTCATCTCCCGGGTATAAAGATGTCACAGTCAATACCTTTCCAGCCCTAATGAGCTTGGATGCTGAAGAGCTGGATCCAATTGAACAGCGAACTTTAAAAATGGAAATGACCCCTGACGTAACCACATTTGCTCTGAGGCGTCTTCTTCGTTTGTTGTACATCGAGCAGTTATCAAGAGCTCGCGTGTCTCCTTTTGTACCCTTTGCAGATACCACAGATGTATCTATTGTAGTTCTAGTGGGATACGAAGCTGTTGGTAAAGGATCCTTTATGCGACGTCTGTCCAAAGAATTTCCTTCAAACATAGAAATTGGCAAACAACACACAACAGCGCTTAACCATCATTCCGTCAATTTCATTGAAATTTCAAGGGACAAATTTGACGACATGCTGCTGGCCGGTGAATTTTTGACTTACGGTGAACGAGACGGCGATTCATATGGGCTAAGTCGTGAGGAAGCTTTTGTGAAGGACGGTAAAGTGAAAATTGTAGCCATGGATTTACTTGGTGCTTTGATGCTGAAGTTACGCGGACTGCGACCGTATTTAATTCTGGCTTCTTACAGAGACAAAGCGACTTTGGCTAAGAGGCAACTCGAGAGAAAAGCGTAA

Protein

MLLELAVYMRQLRRLIVLDLRANPVCSSPGYKDVTVNTFPALMSLDAEELDPIEQRTLKMEMTPDVTTFALRRLLRLLYIEQLSRARVSPFVPFADTTDVSIVVLVGYEAVGKGSFMRRLSKEFPSNIEIGKQHTTALNHHSVNFIEISRDKFDDMLLAGEFLTYGERDGDSYGLSREEAFVKDGKVKIVAMDLLGALMLKLRGLRPYLILASYRDKATLAKRQLERKA

Description

Involved in multiple aspects of sperm assembly including acrosome attachment, shaping of the sperm head and in the early aspects of axoneme development (PubMed:25781171). Not essential for primary cilium biogenesis (PubMed:28003339).
Involved in multiple aspects of sperm assembly including acrosome attachment, shaping of the sperm head and in the early aspects of axoneme development. Not essential for primary cilium biogenesis.

Subunit

Interacts (via guanylate kinase-like domain) with RIMBP3 (via coiled-coil region) (PubMed:28003339). Interacts (via guanylate kinase-like domain) with HOOK2 (PubMed:25781171, PubMed:28003339). Interacts (via LRRCT domain) with KLC3. Interacts with HOOK1 and HOOK3 (PubMed:28003339).
Interacts (via guanylate kinase-like domain) with RIMBP3 (via coiled-coil region). Interacts (via guanylate kinase-like domain) with HOOK2. Interacts (via LRRCT domain) with KLC3. Interacts with HOOK1 and HOOK3.

Miscellaneous

Mutagenesis with N-ethyl-N-nitrosourea (ENU) lead to the discovery of the Kaos phenotype. The Kaos mutation results in the conversion of Arg-528 to a stop codon. Homozygous males are oligoasthenoteratospermic and sterile.

Keywords

ATP-binding   Cell projection   Complete proteome   Cytoplasm   Cytoplasmic vesicle   Cytoskeleton   Differentiation   Kinase   Leucine-rich repeat   Nucleotide-binding   Reference proteome   Repeat   Spermatogenesis   Transferase   Polymorphism  

Feature

chain  Leucine-rich repeat and guanylate kinase domain-containing protein
sequence variant  In dbSNP:rs17167553.

Uniprot

H9JH80   A0A2W1BHK9   A0A212EQ53   A0A194PVH4   A0A2A4J7F0   A0A194RLQ4   + More
A0A2H1VXE9   A0A0L7L405   A0A1B6LGG1   A0A1B6CIR6   A0A1B6EGZ8   A0A1B6CGX8   A0A1B6CRY5   A0A0L7QUH0   A0A1B6F248   A0A151WHL8   V4ASN2   A0A195EXT6   A0A026WL21   A0A195C5J7   A0A151IYS0   A0A1S3JTI2   B3RI90   H0X050   E2AHJ3   A0A369SM54   E0VE51   A0A1W4ZQ51   A0A158NN12   Q9D5S7   A0A195BB55   D4A3R3   A0A088AS02   A0A0G2JWS1   A0A154PFE6   A0A2A3EFI7   A0A1U7T0B1   A0A2U3VRX3   G3SFG5   I3MJ00   A0A087X6K6   A0A3B3UCR6   A0A2R8ZSU8   H2QVE5   Q96M69   F6YA04   A0A1U7QWF7   A0A096ME34   A0A096LRN0  

Pubmed

19121390    28756777    22118469    26354079    26227816    23254933    + More
24508170    18719581    20798317    30042472    20566863    21347285    16141072    25781171    28003339    15057822    15632090    22398555    22722832    16136131    14702039    12690205    15489334   

EMBL

BABH01012769    KZ150370    PZC71153.1    AGBW02013301    OWR43625.1    KQ459591    + More
KPI96993.1    NWSH01002535    PCG68047.1    KQ459989    KPJ18768.1    ODYU01005035    SOQ45527.1    JTDY01003163    KOB70034.1    GEBQ01017174    JAT22803.1    GEDC01023997    JAS13301.1    GEDC01000127    JAS37171.1    GEDC01024551    JAS12747.1    GEDC01020989    JAS16309.1    KQ414736    KOC62111.1    GECZ01025440    JAS44329.1    KQ983115    KYQ47295.1    KB201381    ESO96751.1    KQ981940    KYN32707.1    KK107159    EZA56730.1    KQ978231    KYM96122.1    KQ980745    KYN13665.1    DS985241    EDV28977.1    AAQR03083603    AAQR03083604    AAQR03083605    AAQR03083606    AAQR03083607    AAQR03083608    AAQR03083609    AAQR03083610    GL439548    EFN67096.1    NOWV01000004    RDD47093.1    DS235088    EEB11657.1    ADTU01020827    AK014976    KQ976529    KYM81763.1    AABR07060188    AABR07060189    AABR07060190    CH473959    EDM15296.1    KQ434892    KZC10537.1    KZ288271    PBC29751.1    CABD030055217    CABD030055218    CABD030055219    CABD030055220    CABD030055221    CABD030055222    AGTP01065932    AGTP01065933    AGTP01065934    AYCK01010133    AJFE02042763    AJFE02042764    AACZ04061431    NBAG03000017    PNI99564.1    AK057348    CH236950    CH471070    BC104897    BC104899   

Proteomes

UP000005204    UP000007151    UP000053268    UP000218220    UP000053240    UP000037510    + More
UP000053825    UP000075809    UP000030746    UP000078541    UP000053097    UP000078542    UP000078492    UP000085678    UP000009022    UP000005225    UP000000311    UP000253843    UP000009046    UP000192224    UP000005205    UP000000589    UP000078540    UP000002494    UP000005203    UP000076502    UP000242457    UP000189704    UP000245340    UP000001519    UP000005215    UP000028760    UP000261500    UP000240080    UP000002277    UP000005640    UP000008225    UP000189706   

PRIDE

H9JH80    Q9D5S7    D4A3R3    Q96M69   

Pfam

PF00625   Guanylate_kin        + More
PF12799   LRR_4
PF13855   LRR_8
PF13516   LRR_6
PF00583   Acetyltransf_1

Interpro

IPR032675   LRR_dom_sf        + More
IPR027417   P-loop_NTPase       
IPR008145   GK/Ca_channel_bsu       
IPR008144   Guanylate_kin-like_dom       
IPR025875   Leu-rich_rpt_4       
IPR001611   Leu-rich_rpt       
IPR003591   Leu-rich_rpt_typical-subtyp       
IPR020590   Guanylate_kinase_CS       
IPR000182   GNAT_dom       
IPR016181   Acyl_CoA_acyltransferase       

SUPFAM

SSF52540   SSF52540        + More
SSF55729   SSF55729       

Gene 3D

3.80.10.10    

ProteinModelPortal

H9JH80   A0A2W1BHK9   A0A212EQ53   A0A194PVH4   A0A2A4J7F0   A0A194RLQ4   + More
A0A2H1VXE9   A0A0L7L405   A0A1B6LGG1   A0A1B6CIR6   A0A1B6EGZ8   A0A1B6CGX8   A0A1B6CRY5   A0A0L7QUH0   A0A1B6F248   A0A151WHL8   V4ASN2   A0A195EXT6   A0A026WL21   A0A195C5J7   A0A151IYS0   A0A1S3JTI2   B3RI90   H0X050   E2AHJ3   A0A369SM54   E0VE51   A0A1W4ZQ51   A0A158NN12   Q9D5S7   A0A195BB55   D4A3R3   A0A088AS02   A0A0G2JWS1   A0A154PFE6   A0A2A3EFI7   A0A1U7T0B1   A0A2U3VRX3   G3SFG5   I3MJ00   A0A087X6K6   A0A3B3UCR6   A0A2R8ZSU8   H2QVE5   Q96M69   F6YA04   A0A1U7QWF7   A0A096ME34   A0A096LRN0  

PDB

2QOR     E-value=0.00381714,     Score=92

KEGG

101738145    no KO assigned | (RefSeq) uncharacterized LOC101738145

GO

GO:0016301   GO:0004385   GO:0005829   GO:0001669   GO:0007283   GO:0002177   GO:0035082   GO:0005524   GO:0030154   GO:0042995  

Subcellular location

Cytoplasmic vesicle   Localizes to the acrosome and acroplaxome in round spermatids. Localizes to the manchette during spermiogenesis. Also found in the basal body of elongating spermatids, and in primary cilia of somatic cells.   With evidence from 2 publications.
Secretory vesicle   Localizes to the acrosome and acroplaxome in round spermatids. Localizes to the manchette during spermiogenesis. Also found in the basal body of elongating spermatids, and in primary cilia of somatic cells.   With evidence from 2 publications.
Acrosome   Localizes to the acrosome and acroplaxome in round spermatids. Localizes to the manchette during spermiogenesis. Also found in the basal body of elongating spermatids, and in primary cilia of somatic cells.   With evidence from 2 publications.
Cytoplasm   Localizes to the acrosome and acroplaxome in round spermatids. Localizes to the manchette during spermiogenesis. Also found in the basal body of elongating spermatids, and in primary cilia of somatic cells.   With evidence from 2 publications.
Cytoskeleton   Localizes to the acrosome and acroplaxome in round spermatids. Localizes to the manchette during spermiogenesis. Also found in the basal body of elongating spermatids, and in primary cilia of somatic cells.   With evidence from 2 publications.
Cilium basal body   Localizes to the acrosome and acroplaxome in round spermatids. Localizes to the manchette during spermiogenesis. Also found in the basal body of elongating spermatids, and in primary cilia of somatic cells.   With evidence from 2 publications.

Length:

229

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.24517

Exp number, first 60 AAs:

0

Total prob of N-in:

0.03618

outside

1  -  229

 

 

Pi

240.393683

Theta

172.938013

Tajima's D

1.405902

CLR

0

CSRT

0.765911704414779

Interpretation

Uncertain