Silkworm Genome Informatics Database

Gene

KWMTBOMO01301

Pre Gene Modal

BGIBMGA009014

Annotation

PREDICTED:_cytochrome_b5_reductase_4_isoform_X1_[Amyelois_transitella]

Location

Bomo_Chr3(-):7635510-7678434 View in EpiBrowser

Full name

Cytochrome b5 reductase 4

Alternative Name

Flavohemoprotein b5/b5R
N-terminal cytochrome b5 and cytochrome b5 oxidoreductase domain-containing protein
cb5/cb5R

Location in the cell

Mitochondrial Reliability : 1.917 Nuclear Reliability : 1.282

CDS

ATGGACTGGATCAGACTGGGCAACTCCGGCAAAGATCTGACCGGCGTTGGAGGACGGATACGACCCGTCACCCACACCGAATTAGCGGCACACAACACTCAAAAAGATGCGTGGCTAGCCATCCGCGGTCGGGTTTATAACATAACCCACTACTTACCTTATCACCCTGGAGGCCCCGAAGAATTAATGCGTGGCGCAGGAATGGACGCCACCCAATTATTCGATAAAGTCCATCCTTGGGTGAACTACGACTCGCTTCTGGCCAAATGTTTAGTAGGCCCTCTGCGCTATGATCTACCCGATGCAGAAGAACTCTTCGACACCTCCTCACCATCCTCAAAATCGGACCGCCTCAGAGAACCTTCTAAAGCACAAGAATTAGTCAGAAAATCTATGGAGAATTTAGCTAATTGTATTACGCCCGTAAGAAAGAAAATATCAAATAAAAGCGAAGAAAACGCCAAAGGTAGCCCGCCGAGTAAAATAATGCAGAGTCTGATACAATCTAGCGACTTACCGGTGTCTATAAGCCGGCGAGCAGCAGCGAGTCCTGTGAAGAAAGCCGATAAAAATACAGAAACTCCCACCCCTATGCGATACGATTGGATTCAAACATCTACAAAGCTAACAATCTCCGTTTACACGGGTCCGCTGGCAAATCCTGGTGGATGTGCGAGGATTACCGACGGCTTCCTATTGATCGAAGTAGCAACGAACGGTTGGCTTAGGACACTTAAAATAACACCTGAGGCGACTTTGCAGGAACCTTTGCAGCTACGGGTGTTTGCAGAGAGTGGTAAAGTAGAGGTAATTGCACGTAAAGTGGAAAGCAGTCTCTGGAAAGGCTGCGGTGAAGTAGTCCTAGGTGTGCCAAAGAAAATCTCTTCGCCTAGAACAATGGAATGTCGTATAATGCGAGTAGATCGTGTGTCACACGACACTACATTGCTTTCGTTGACACCTAAGGCTGGTCCTGTTCTCGTGCCTCTCGGGCACCACGTACGGATACACGAGCAAATGGAAGGTTCTGAATTAGTCAGATCATATACTCCCGTAGGCGAAGGTTGGGGTGCAACTGATGACGTGCTTTCAGCTTTAAGATTAGCAGTGAAGAAATATGACTCAGGTGCAATGTCTCCGCTCTTGGCCTCTCTGAAGATTAGTGACGTGGTTACGTTATCCGGACCATATGGAAATTTTGAATTATCAAAGTTGAAAACGGTGAAGGTAATGTATCTTATAGCCGCTGGGACCGGCATTACTCCGATGCTCGGCCTCGTGAAGTTTATGCTGGCGAGATCTAACCCCAGATGTGAACGGGTGCATCTGCTTTTCTTTAACAAAACAGAACAAGACATATTATTTAATGATATCCTTGAAGATATAGCGAAGGAGGACGATAGATTTGTTGTAACAAATATTTTATCCAATGCTGGTCCATCATGGATAGGACACACAGGAAGAATATCCAAAGAACTACTTAAAAACATATTCGATAAAGATTCCATGAAATGTTATACAAAATGTACTCATTACGCTTGCATTTGTGGACCAAATGAGTTCACACATACTTCACTAGATTTGTTGAAACAAGAAGGCATGAAAGATAATTGTTTGCATGCGTTTATGGGCTAA

Protein

MDWIRLGNSGKDLTGVGGRIRPVTHTELAAHNTQKDAWLAIRGRVYNITHYLPYHPGGPEELMRGAGMDATQLFDKVHPWVNYDSLLAKCLVGPLRYDLPDAEELFDTSSPSSKSDRLREPSKAQELVRKSMENLANCITPVRKKISNKSEENAKGSPPSKIMQSLIQSSDLPVSISRRAAASPVKKADKNTETPTPMRYDWIQTSTKLTISVYTGPLANPGGCARITDGFLLIEVATNGWLRTLKITPEATLQEPLQLRVFAESGKVEVIARKVESSLWKGCGEVVLGVPKKISSPRTMECRIMRVDRVSHDTTLLSLTPKAGPVLVPLGHHVRIHEQMEGSELVRSYTPVGEGWGATDDVLSALRLAVKKYDSGAMSPLLASLKISDVVTLSGPYGNFELSKLKTVKVMYLIAAGTGITPMLGLVKFMLARSNPRCERVHLLFFNKTEQDILFNDILEDIAKEDDRFVVTNILSNAGPSWIGHTGRISKELLKNIFDKDSMKCYTKCTHYACICGPNEFTHTSLDLLKQEGMKDNCLHAFMG

Description

NADH-cytochrome b5 reductase involved in endoplasmic reticulum stress response pathway. Plays a critical role in protecting pancreatic beta-cells against oxidant stress, possibly by protecting the cell from excess buildup of reactive oxygen species (ROS).
NADH-cytochrome b5 reductase involved in endoplasmic reticulum stress response pathway.

Catalytic Activity

2 [Fe(III)-cytochrome b5] + NADH = 2 [Fe(II)-cytochrome b5] + H(+) + NAD(+)

Cofactor

FAD

Biophysicochemical Properties

25 mM for O(2)

Similarity

Belongs to the cytochrome b5 family.
Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.

Keywords

Acetylation Alternative splicing Complete proteome Endoplasmic reticulum FAD Flavoprotein Heme Iron Metal-binding NAD Oxidoreductase Polymorphism Reference proteome 3D-structure

Feature

chain  Cytochrome b5 reductase 4
splice variant  In isoform 2.
sequence variant  In dbSNP:rs61762820.

Uniprot

H9JHL6 A0A2H1VWI4 A0A2A4J1D8 A0A2W1BMG5 A0A212ESP9 A0A194Q0R5 + More
A0A194R9A2 A0A194RD55 A0A194PUB3 A0A1Y1KAD7 D6WYW5 A0A182WFM8 A0A182QPT9 A0A2M4BJG0 A0A182FLI8 W8BMG6 W8BHF5 A0A182Y6Y3 A0A182RLL4 A0A182N3E6 W5NIK3 W5NIK4 A0A182LHC0 A0A182LT14 A0A2M4CLH8 A0A1L8DZU9 A0A2M4CH98 K1R3R9 A0A2M4CHS2 A0A2M4CH99 A0A2M4AUB6 A0A182J5T3 A0A0K8VDC3 A0A0K8U8V0 W5UKK7 V9KPJ0 A0A2D0RK54 A0A182HY71 A0A091INN2 A0A087X649 A0A182VLE9 A0A1X7UG22 A0A3B1J0Y5 A0A182G438 Q3TDX8 A0A091W7B5 I3LV78 A0A182P0T9 A0A2K6QN53 H3ASV7 A0A3B5Q838 A0A091RA38 M4AJA2 F7DDM6 A0A3B5R1X9 U6CTX3 G7P3I7 A0A146VNU9 A0A2K5Z0F1 F7ADF0 G1PFU7 F7HJT5 S7N218 F7DDA0 A0A096NUI0 A0A2K5TRG2 A0A2K6D4F9 A0A091G9J9 A0A093TJP3 A0A091H646 A0A2U3WH08 A0A3B3QID0 A0A091IQX7 B2R7W7 A0A091QXS2 A0A2R9CF58 H2QTC4 Q7L1T6 G2HEA3 A0A3B3QJ30 A0A094KI93 A0A1L8G3B8 Q502I6 L8IHQ7 G1RQH5 W5JV99 A0A369SIU6 A0A091NE05 F1QIA4 G3QK98 A0A3B3QI07 A0A1V4JMA6 A0A0A0ALF7 B4KT92 A0A1S3RWS7 A0A1S3NHW6 A0A1E1X9K2 A0A0P4W9D4 A0A1S3RXT0

EC Number

1.6.2.2

Pubmed

19121390 28756777 22118469 26354079 28004739 18362917 + More
19820115 24495485 25244985 20966253 22992520 23127152 24402279 25329095 26483478 16141072 19468303 15489334 10611283 14962668 15247412 16814408 25362486 9215903 23542700 19892987 22002653 17431167 21993624 25243066 29240929 22722832 16136131 14574404 22814378 20630863 15504981 15131110 16959974 21484476 27762356 22751099 20920257 23761445 30042472 23594743 22398555 17994087 18057021 28503490

EMBL

BABH01012801 ODYU01004705 SOQ44872.1 NWSH01004310 PCG65232.1 KZ150048 + More
PZC74467.1 AGBW02012746 OWR44507.1 KQ459591 KPI97000.1 KQ460597 KPJ13840.1 KPJ13841.1 KPI97001.1 GEZM01087897 JAV58409.1 KQ971363 EFA07854.2 AXCN02001110 GGFJ01003950 MBW53091.1 GAMC01008384 GAMC01008382 JAB98173.1 GAMC01008383 JAB98172.1 AHAT01009243 AHAT01009244 AHAT01009245 AHAT01009246 AXCM01001435 AXCM01001436 GGFL01002012 MBW66190.1 GFDF01002165 JAV11919.1 GGFL01000539 MBW64717.1 JH817581 EKC38069.1 GGFL01000724 MBW64902.1 GGFL01000538 MBW64716.1 GGFK01011055 MBW44376.1 GDHF01015764 JAI36550.1 GDHF01029296 GDHF01027574 JAI23018.1 JAI24740.1 JT417830 AHH42516.1 JW868071 AFP00589.1 APCN01004497 KK500574 KFP09005.1 AYCK01024375 AYCK01024376 AYCK01024377 JXUM01006928 JXUM01006929 JXUM01006930 JXUM01006931 KQ560227 KXJ83737.1 AK078682 AK090159 AK157312 AK167436 AK169937 AK172252 AC156793 BC025438 BC002170 AF338818 AY321368 AY321369 KK734920 KFR11517.1 AEMK02000001 AFYH01061691 AFYH01061692 AFYH01061693 AFYH01061694 AFYH01061695 AFYH01061696 AFYH01061697 AFYH01061698 AFYH01061699 AFYH01061700 KK715023 KFQ35784.1 HAAF01001466 CCP73292.1 CM001279 EHH53210.1 GCES01067694 JAR18629.1 JSUE03031461 CM001256 EHH18505.1 AAPE02053075 AAPE02053076 AAPE02053077 AAPE02053078 AAPE02053079 GAMT01005119 GAMS01004249 GAMR01008507 GAMQ01004520 GAMP01004993 JAB06742.1 JAB18887.1 JAB25425.1 JAB37331.1 JAB47762.1 KE162930 EPQ10030.1 AHZZ02025161 AHZZ02025162 AQIA01050963 AQIA01050964 AQIA01050965 AQIA01050966 KL447974 KFO79050.1 KL442178 KFW94489.1 KL523126 KFO90292.1 KL218085 KFP02061.1 AK313146 BAG35964.1 KK684822 KFQ14144.1 AJFE02055677 AJFE02055678 AJFE02055679 AACZ04027281 GABC01004098 GABF01005424 GABE01010288 NBAG03000210 JAA07240.1 JAA16721.1 JAA34451.1 PNI88022.1 EU448291 AL034347 AL139232 BC025380 AF169803 AK305067 BAK62061.1 KL348158 KFZ57132.1 CM004475 OCT78306.1 BC095683 JH881309 ELR55069.1 ADFV01180245 ADFV01180246 ADFV01180247 ADFV01180248 ADFV01180249 ADFV01180250 ADFV01180251 ADFV01180252 ADMH02000250 ETN67243.1 NOWV01000012 RDD45933.1 KL382073 KFP87958.1 CABZ01043952 LO017673 CABD030046379 CABD030046380 CABD030046381 CABD030046382 CABD030046383 LSYS01006902 OPJ73336.1 KL872360 KGL95319.1 CH933808 EDW10604.1 KRG05344.1 GFAC01003269 JAT95919.1 GDRN01089823 JAI60595.1

Proteomes

UP000005204 UP000218220 UP000007151 UP000053268 UP000053240 UP000007266 + More
UP000075920 UP000075886 UP000069272 UP000076408 UP000075900 UP000075884 UP000018468 UP000075882 UP000075883 UP000005408 UP000075880 UP000221080 UP000075840 UP000053119 UP000028760 UP000075903 UP000007879 UP000018467 UP000069940 UP000249989 UP000000589 UP000053605 UP000008227 UP000075885 UP000233200 UP000008672 UP000002852 UP000002281 UP000009130 UP000233140 UP000006718 UP000001074 UP000008225 UP000028761 UP000233100 UP000233120 UP000053760 UP000245340 UP000261540 UP000054308 UP000240080 UP000002277 UP000005640 UP000186698 UP000000437 UP000001073 UP000000673 UP000253843 UP000001519 UP000190648 UP000053858 UP000009192 UP000087266

PRIDE

H9JHL6 Q3TDX8 B2R7W7 Q7L1T6 Q502I6

Pfam

PF00970   FAD_binding_6        + More
PF00173   Cyt-b5
PF00175   NAD_binding_1
PF04969   CS

Interpro

IPR008333   Cbr1-like_FAD-bd_dom        + More
IPR039261   FNR_nucleotide-bd
IPR018506   Cyt_B5_heme-BS
IPR001834   CBR-like
IPR017938   Riboflavin_synthase-like_b-brl
IPR036400   Cyt_B5-like_heme/steroid_sf
IPR001199   Cyt_B5-like_heme/steroid-bd
IPR017927   FAD-bd_FR_type
IPR001433   OxRdtase_FAD/NAD-bd
IPR008978   HSP20-like_chaperone
IPR007052   CS_dom
IPR037908   p23_NCB5OR
IPR001709   Flavoprot_Pyr_Nucl_cyt_Rdtase

SUPFAM

SSF63380   SSF63380        + More
SSF52343   SSF52343
SSF55856   SSF55856
SSF49764   SSF49764

Gene 3D

3.10.120.10 3.40.50.80 2.60.40.790

CDD

cd06490 p23_NCB5OR

ProteinModelPortal

H9JHL6 A0A2H1VWI4 A0A2A4J1D8 A0A2W1BMG5 A0A212ESP9 A0A194Q0R5 + More
A0A194R9A2 A0A194RD55 A0A194PUB3 A0A1Y1KAD7 D6WYW5 A0A182WFM8 A0A182QPT9 A0A2M4BJG0 A0A182FLI8 W8BMG6 W8BHF5 A0A182Y6Y3 A0A182RLL4 A0A182N3E6 W5NIK3 W5NIK4 A0A182LHC0 A0A182LT14 A0A2M4CLH8 A0A1L8DZU9 A0A2M4CH98 K1R3R9 A0A2M4CHS2 A0A2M4CH99 A0A2M4AUB6 A0A182J5T3 A0A0K8VDC3 A0A0K8U8V0 W5UKK7 V9KPJ0 A0A2D0RK54 A0A182HY71 A0A091INN2 A0A087X649 A0A182VLE9 A0A1X7UG22 A0A3B1J0Y5 A0A182G438 Q3TDX8 A0A091W7B5 I3LV78 A0A182P0T9 A0A2K6QN53 H3ASV7 A0A3B5Q838 A0A091RA38 M4AJA2 F7DDM6 A0A3B5R1X9 U6CTX3 G7P3I7 A0A146VNU9 A0A2K5Z0F1 F7ADF0 G1PFU7 F7HJT5 S7N218 F7DDA0 A0A096NUI0 A0A2K5TRG2 A0A2K6D4F9 A0A091G9J9 A0A093TJP3 A0A091H646 A0A2U3WH08 A0A3B3QID0 A0A091IQX7 B2R7W7 A0A091QXS2 A0A2R9CF58 H2QTC4 Q7L1T6 G2HEA3 A0A3B3QJ30 A0A094KI93 A0A1L8G3B8 Q502I6 L8IHQ7 G1RQH5 W5JV99 A0A369SIU6 A0A091NE05 F1QIA4 G3QK98 A0A3B3QI07 A0A1V4JMA6 A0A0A0ALF7 B4KT92 A0A1S3RWS7 A0A1S3NHW6 A0A1E1X9K2 A0A0P4W9D4 A0A1S3RXT0

PDB

3LF5 E-value=2.68588e-23, Score=270

KEGG

101738678 K00326 cytochrome-b5 reductase [EC:1.6.2.2] | (RefSeq) cytochrome b5 reductase 4

PATHWAY

00520 Amino sugar and nucleotide sugar metabolism - Bombyx mori (domestic silkworm)

GO

GO:0016491 GO:0046872 GO:0020037 GO:0016021 GO:0004128 GO:0055114 GO:0005783 GO:0006801 GO:0006091 GO:0016653 GO:0030073 GO:0016174 GO:0042168 GO:0005737 GO:0006739 GO:0003958 GO:0048471 GO:0048468 GO:0050660 GO:0046677 GO:0042593 GO:0015701 GO:0005789 GO:0003032 GO:0000287 GO:0015934 GO:0005576 GO:0016042 GO:0005634 GO:0006334 GO:0003779

Subcellular location

Endoplasmic reticulum Soluble protein. With evidence from 1 publications.

Length:

544

Number of predicted TMHs:

1

Exp number of AAs in TMHs:

18.21198

Exp number, first 60 AAs:

0.00038

Total prob of N-in:

0.00758

outside

1 - 409

TMhelix

410 - 432

inside

433 - 544

Pi

246.641816

Theta

175.798129

Tajima's D

1.414148

CLR

0.417238

CSRT

0.770461476926154

Interpretation

Uncertain

Sequence

Summary

Ontologies

Topology

Population Genetic Test Statistics