Gene

KWMTBOMO01293

Annotation

reverse_transcriptase_[Bombyx_mori]

Location

Bomo_Chr3(+):7569175-7573400      View in EpiBrowser

Full name

RNA-directed DNA polymerase from mobile element jockey       + More
Probable RNA-directed DNA polymerase from transposon BS      

Alternative Name

Reverse transcriptase

Location in the cell

Nuclear   Reliability : 3.033

CDS

ATGAACAGCAGCAGATCTTTCGTCTTATTGAATTGTGTACAACAGGAACAACATAACGGCAAGGACCGCGAGCTCCGCGTAGTAATACGCGGAGTACCGAAAGAACTAGACATAGACTACGTTAAGGAGGACCTGATCGCTCAGGCACTCCCTATAGTTAGTGTGCATCGGATGCACAGTGGGCGGGGCAAGCAGCCCTACAACATGATACTCGTCGCGTTGGAGCCAACCCCGGAGGCAAAAAAGAAAATCTCATGCCTCTCGACAGTTTGCGGCCTATCCGGGGTCTCCATCGAAGCCCCCCATAAACGTGGCACTCCCGGGCAGTGCTACAGATGCCAGCTTTACGGCCACTCGGCTCGTAATTGCCACGCGCGCCCCCGCTGCGTAAAGTGCCTCGGCGACCACGCCACATTAGATTGCTCGCGAGTTAGGGAAACCGCGACCGAACCTCCAAGCTGTGTCCTATGCCTTAAGCAAGGGCACCCCGCGAACTACCGTGGATGTCCTAGGGCTCCGCGAAAACGTCCAACTCACCCGGCTCCGCGAACCGTCGGCCCAAAGACTTCGGCACCTTCAGTGCCGAAACCCGCCTTCGTGCCGGCTGCAGTTCCCACGGTCTCGGCGTGGAAAAAACCGCTGCCGTATACTAAGGCGGGAACGGAAACCGCACCCCCGCCCCCGCTCGTGACACGCCCCGCGCCCCAGCCTCTGCTCGCACCTCGCCCCGCGCCCGCGTTCCGTCCCCCGCAACCCTCTGCCGTCAACGACTTCGCGCTCGTGCGCGACTTCGTCACCGCGGTGAACTTCGACCGTCTGCGATCGTTCGCTGACGCTATCCGTAGGTCAGGAACCCCCGAACAGCGGCTCGCGGCCGCCTTCGACCACATGGACGTTTACGAGTCCGTGTCGCCATTCTACAATGCTAACGGACTCGCGCGGCAACGCGATCAAATTTTCGAATTCCTCCGCGACAATCTTATAGATATCTTGTTAGTGCAGGAGACCTGTCTGAAGCCCTCGCGTCGCGACCCGAAAGTCGCGAACTACGTCATGGTTAGGAATGACAGACTCACCGCCTCGAAAGGCGGGACTGCCATTTACTATAGACGGGCCCTGCACGTTGTCCCTCTCGATACTCCCTCGCTCTTACATATCGAGGCGTCAGTGTGCCGTATCTCGCTGACGGGACACCAGCCGATCGTCATCGCATCCGTTTATCTCCCCCCGGACAAGCCCCTCCTGAGCAGTGACATCGAGTCACTGTTCGGCATGGGAGACTCCGTCATCCTGGCAGGCGATTTAAATTGCCACCACACTAGGTGGAACTGCCATCGTACAAACGTTAACGGTAGGCGTCTCGACGCGTTTATAGACGACCTCACCTTTGAAATAGTCGGTCCCCCAACTCCAACATGTTATCCGTATAACATCGCGCTCCGTCCGAGCACTATAGACCTGGCATTGCTTAGGAACGTAACTCTGCGCTTGCGTTCCATCGAAGCAATGTCAGAGCTCGACTCAGACCACCGACCTGTCGTTATGCAGCTCGGTCGCCCTCACAACCCAGTCACTGTTACGAGGACCATGGTGGATTGGAATAAGCTGGGCACGTGCCTAGCCGACGCCGCTCCGCCAATCCTCCCTTACGGCCCGGATTCGAATCCATCCCCCGAGGACACCGTCGAATCCATTAACATCATTACCGATCACATCTCTTCCGCGATCATTAGATCTTCTAAAGAAGTCGATGTGGAGGACAGCTTCCACCGCATCAGACTGTCCCCCGATCTTAGGAATCTCTTAAGAGTTAGGAACGCGGCAATCCGGGCCTACGATCGTCTTCCCACGCATTCAAACCGGATTCAGATGCGTCGTCTACAACGCGAAGTCCACTCCCGCTTAAGCGACGCGCGTAACGATAATTGGCATAGTTATTTAGAACAACTCGCGCCCTCCCACCAAGCATACTGGCGACTAGCTAGGACTCTCAAATCCGAAACTACCGCTACTATGCCTCCCCTCTTACGCCCTTCAGGCCAACCACCGGCATTCGATGACGATGACAAGGCTGAGCTGCTGGCCGATGCACTGCAAGAGCAGTGCACCACCAGCACTCAACACGCGGACCCCGAACACACCGAGTTAGTCGACAGGGAGGTCGAGCGCAGAGCTTCCCTGCCGCCCTCGGACGCGTTACCCCCCATTACCACTGACGAAGTTAGAGACGCGATCCACAACCTCCAACCTAGGAAGGCACCAGGCTCCGACGGCATCCACAACCGCGCGCTAAAATTTTTGCCAGTCCAACTGATAGCAATGTTGGCTACAATTTTAAATGCCGCTATGACGCACTGCATCTTTCCCGCGGTGTGGAAAGAAGCGGACGTTATCGGTATACATAAGCCGGGCAAACCGAGAAACGAAACTTCTAGTTACCGTCCGATTAGTCTCCTCTCGACGATAGGAAAAATTTACGAACGTCTCCTTAGGAAACGCCTCTGGGATTTTGTTACCGCGAACAAAATTCTCATAGACGAACAGTTCGGATTCCGCTCAAAACACTCGTGCGTACAACAAGTGCACCGCCTCACGGAGCACATTCTGATAGGACTAAATAGGCGTAAACAAATCCCGACCGGCGCCCTCTTCTTCGACATCGCGAAGGCGTTCGACAAAGTCTGGCACAACGGTTTGATCTACAAACTGTACAACATGGGAGTGCCAGACAGACTCGTGCTCATCATACGAGACTTCTTGTCGAACCGTTCGTTTCGATATCGAGTAGAGGGAACTCGTTCTCGTCCCCGTCAACTGACTGCCGGAGTCCCGCAAGGCTCCGCGCTCTCCCCGTTATTATTTAGTTTGTATATCAATGATATACCCCGGTCTCCGGAGACCCATCTAGCGCTCTTCGCCGACGACACGGCTATCTACTACTCGTGTAGGAAGATGTCGCTGCTTCATCGGCGACTCCAGATCGCAGTAGCCACCATGGGACAGTGGTTCCGGAAGTGGCGAATAGACATCAACCCCACGAAAAGCGCAGCGGTGCTCTTCAAAAGGGGTCGCCCTCCGAATATCACTTCGAGCATCCCACTCCGTAGTAGGCGCGCAAACACCTCCGCCGTTAGTCCCATCACTCTCTTTGGCCAGCCCATACCGTGGGTCTCGAAGGTCAAATATCTAGGCGTCACCCTCGACAGAGGGATGACATTCCGTCTCCATATAAAAACGGTACGCGACCGCGCCGCGTTTATTCTAGGACGACTCTATCCAATGCTTTGTAGTCGAAGCAAACTGTCCCTCCGCAATAAGGTAACTCTCTACAAAACTTGTATACGCCCCGTCATGACGTATGCAAGCGTAGTGTTCGCTCACTCAGCCCGCACCCACTTGAAATCCCTTCAGGTTATTCAATCACGATTCTGCAGGATAGCCGTCGGAGCGCCATGGTTCCTTAGGAATGTGGATCTCCACGATGACCTGGAGCTCGACTCTCTTAGTAAGTATCTACAGTCGGCATCACTGCGCCATTTTGAGAAGGCGGCACGACATGAGAACCCTCTCATCGTAGCCGCTGGAAATTACATACCCGACCCAGTAGACCGAATGGTAAACCGTCGACGTCGCCCAAAGCACGTCATTACGGATCCTCCTGATCCATTAACGGTGCTTTTAGGCACCACAAGCACCGGTCACCGTCCTCGTCGAACCCGTCGCTTGCGACGAAGGGCTCGACGAGCGAACTAA

Protein

MNSSRSFVLLNCVQQEQHNGKDRELRVVIRGVPKELDIDYVKEDLIAQALPIVSVHRMHSGRGKQPYNMILVALEPTPEAKKKISCLSTVCGLSGVSIEAPHKRGTPGQCYRCQLYGHSARNCHARPRCVKCLGDHATLDCSRVRETATEPPSCVLCLKQGHPANYRGCPRAPRKRPTHPAPRTVGPKTSAPSVPKPAFVPAAVPTVSAWKKPLPYTKAGTETAPPPPLVTRPAPQPLLAPRPAPAFRPPQPSAVNDFALVRDFVTAVNFDRLRSFADAIRRSGTPEQRLAAAFDHMDVYESVSPFYNANGLARQRDQIFEFLRDNLIDILLVQETCLKPSRRDPKVANYVMVRNDRLTASKGGTAIYYRRALHVVPLDTPSLLHIEASVCRISLTGHQPIVIASVYLPPDKPLLSSDIESLFGMGDSVILAGDLNCHHTRWNCHRTNVNGRRLDAFIDDLTFEIVGPPTPTCYPYNIALRPSTIDLALLRNVTLRLRSIEAMSELDSDHRPVVMQLGRPHNPVTVTRTMVDWNKLGTCLADAAPPILPYGPDSNPSPEDTVESINIITDHISSAIIRSSKEVDVEDSFHRIRLSPDLRNLLRVRNAAIRAYDRLPTHSNRIQMRRLQREVHSRLSDARNDNWHSYLEQLAPSHQAYWRLARTLKSETTATMPPLLRPSGQPPAFDDDDKAELLADALQEQCTTSTQHADPEHTELVDREVERRASLPPSDALPPITTDEVRDAIHNLQPRKAPGSDGIHNRALKFLPVQLIAMLATILNAAMTHCIFPAVWKEADVIGIHKPGKPRNETSSYRPISLLSTIGKIYERLLRKRLWDFVTANKILIDEQFGFRSKHSCVQQVHRLTEHILIGLNRRKQIPTGALFFDIAKAFDKVWHNGLIYKLYNMGVPDRLVLIIRDFLSNRSFRYRVEGTRSRPRQLTAGVPQGSALSPLLFSLYINDIPRSPETHLALFADDTAIYYSCRKMSLLHRRLQIAVATMGQWFRKWRIDINPTKSAAVLFKRGRPPNITSSIPLRSRRANTSAVSPITLFGQPIPWVSKVKYLGVTLDRGMTFRLHIKTVRDRAAFILGRLYPMLCSRSKLSLRNKVTLYKTCIRPVMTYASVVFAHSARTHLKSLQVIQSRFCRIAVGAPWFLRNVDLHDDLELDSLSKYLQSASLRHFEKAARHENPLIVAAGNYIPDPVDRMVNRRRRPKHVITDPPDPLTVLLGTTSTGHRPRRTRRLRRRARRAN

Catalytic Activity

a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)

Cofactor

Mg(2+)
Mn(2+)

Miscellaneous

Prefers poly(rC) and poly(rA) as template and activated DNA is not effective.

Keywords

Nucleotidyltransferase   RNA-directed DNA polymerase   Transferase   Transposable element  

Feature

chain  RNA-directed DNA polymerase from mobile element jockey

Uniprot

Q9XXW0   Q93137   Q9BPQ0   A0A0N1PFV1   O96546   A0A1Y1NA23   + More
A0A139WA11   V5GNJ2   V5GNF3   X1WLB2   J9LFC9   A0A087UGF5   A0A2M4BD67   A0A2M4BDV6   A0A2S2QEE0   X1WZH0   A0A2J7R7D0   A0A2S2N814   A0A069DZL8   A0A2S2NGV8   A0A224X6S1   A0A224X746   A0A2H8TF37   A0A224XI99   A0A224X6U5   P21328   A0A2J7PDM0   A0A224X6R6   A0A0A9YJ00   A0A0A9YU04   A0A2J7QB68   A0A2J7PVX3   A0A2S2P698   A0A2S2R9D4   A0A2A4IUM2   A0A224XHW8   A0A1B6EGD8   A0A2S2NPG3   A0A224XJI0   A0A2S2PJG2   Q95SX7  

EC Number

2.7.7.49

Pubmed

10333566    26354079    10380107    28004739    18362917    19820115    + More
26334808    2463954    3040362    1714378    25401762    7753626    12537569   

EMBL

AB018558    BAA76304.1    U07847    AAA17752.1    AB055391    BAB21761.1    + More
KQ459265    KPJ02064.1    AF081103    AAC72921.1    GEZM01008457    JAV94772.1    KQ971729    KYB24751.1    GALX01005299    JAB63167.1    GALX01005359    JAB63107.1    ABLF02042518    ABLF02057746    ABLF02023911    KK119692    KFM76444.1    GGFJ01001858    MBW50999.1    GGFJ01001857    MBW50998.1    GGMS01006359    MBY75562.1    ABLF02034467    NEVH01006736    PNF36716.1    GGMR01000676    MBY13295.1    GBGD01000415    JAC88474.1    GGMR01003407    MBY16026.1    GFTR01008271    JAW08155.1    GFTR01008151    JAW08275.1    GFXV01000908    MBW12713.1    GFTR01008256    JAW08170.1    GFTR01008251    JAW08175.1    M22874    M38643    NEVH01026386    PNF14434.1    GFTR01008269    JAW08157.1    GBHO01010547    GBHO01010546    JAG33057.1    JAG33058.1    GBHO01021814    GBHO01010549    JAG21790.1    JAG33055.1    NEVH01016302    PNF25823.1    NEVH01020940    PNF20481.1    GGMR01012305    MBY24924.1    GGMS01017365    MBY86568.1    NWSH01007753    PCG62823.1    GFTR01008254    JAW08172.1    GEDC01000337    JAS36961.1    GGMR01006408    MBY19027.1    GFTR01008257    JAW08169.1    GGMR01016905    MBY29524.1    AY060438    AAL25477.1   

Proteomes

UP000053268    UP000007266    UP000007819    UP000054359    UP000235965    UP000218220    + More

PRIDE

Q9XXW0    Q93137    Q9BPQ0    P21328    Q95SX7   

Pfam

PF14529   Exo_endo_phos_2        + More
PF00078   RVT_1
PF07530   PRE_C2HC
PF03372   Exo_endo_phos

Interpro

IPR000477   RT_dom        + More
IPR005135   Endo/exonuclease/phosphatase       
IPR036691   Endo/exonu/phosph_ase_sf       
IPR006579   Pre_C2HC_dom       

SUPFAM

SSF56219   SSF56219       

Gene 3D

3.60.10.10    

ProteinModelPortal

Q9XXW0   Q93137   Q9BPQ0   A0A0N1PFV1   O96546   A0A1Y1NA23   + More
A0A139WA11   V5GNJ2   V5GNF3   X1WLB2   J9LFC9   A0A087UGF5   A0A2M4BD67   A0A2M4BDV6   A0A2S2QEE0   X1WZH0   A0A2J7R7D0   A0A2S2N814   A0A069DZL8   A0A2S2NGV8   A0A224X6S1   A0A224X746   A0A2H8TF37   A0A224XI99   A0A224X6U5   P21328   A0A2J7PDM0   A0A224X6R6   A0A0A9YJ00   A0A0A9YU04   A0A2J7QB68   A0A2J7PVX3   A0A2S2P698   A0A2S2R9D4   A0A2A4IUM2   A0A224XHW8   A0A1B6EGD8   A0A2S2NPG3   A0A224XJI0   A0A2S2PJG2   Q95SX7  

PDB

6AR3     E-value=0.00109977,     Score=105

KEGG

105842032    no KO assigned | (RefSeq) uncharacterized LOC105842032

GO

GO:0003964   GO:0004519   GO:0006313   GO:0003676   GO:0008270   GO:0000287   GO:0015934   GO:0005576   GO:0016042   GO:0005634   GO:0006334   GO:0003779   GO:0016021  

Length:

1250

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.510630000000001

Exp number, first 60 AAs:

0

Total prob of N-in:

0.00003

outside

1  -  1250

 

 

Pi

151.575629

Theta

11.39177

Tajima's D

0.521378

CLR

0.786875

CSRT

0.535023248837558

Interpretation

Uncertain