Gene

KWMTBOMO01225

Pre Gene Modal

BGIBMGA009037

Annotation

argonaute_3_[Bombyx_mori]

Location

Bomo_Chr3(-):5538557-5540577      View in EpiBrowser

Full name

Piwi-like protein Ago3      

Location in the cell

Nuclear   Reliability : 2.829

CDS

ATGGCAGACCCAGGCAAAGGCCGGGGGCGAAGCTTAGCCCTTTTACAGGCACTGAAGAAGTCACAAATGATGGACTCTCCCTCACAATCTGAGAGTCAGTCTCCTGAAAGTACACCAGAACAGAGTACAGCTCCAAGTACCATTGCAAGTGCAACTCCTAGTACTTCAGGAGTGAGTATAGGCGGTAGAGGACGCGCAGCAGCATTGATGCTGGCTAAAATGCAGCAGAAACCAGGCTCCACAACTCCAGCAATTTTTGTGCCACCTTCATCAACTTCTGCACCAACAGCTGGAACTGGACGTGGATTCAAATTGCTTCAGAGCTTGTGA

Protein

MADPGKGRGRSLALLQALKKSQMMDSPSQSESQSPESTPEQSTAPSTIASATPSTSGVSIGGRGRAAALMLAKMQQKPGSTTPAIFVPPSSTSAPTAGTGRGFKLLQSL

Description

Endoribonuclease that plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity (PubMed:19460866). Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells (PubMed:19460866, PubMed:25558067). Its presence in oocytes suggests that it may participate in similar functions during oogenesis in females (PubMed:18191035). Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons (PubMed:19460866, PubMed:25558067). Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements (PubMed:19460866, PubMed:25558067). Strongly prefers a have adenine at position 10 of their guide (g10A preference) (PubMed:24757166, PubMed:25558067). Plays a key role in the piRNA amplification loop, also named ping-pong amplification cycle: antisense piRNA-bound Siwi and sense piRNA-bound Ago3 reciprocally cleave complementary transcripts, to couple the amplification of piRNAs with the repression of transposable elements (PubMed:25558067).

Cofactor

Mg(2+)

Subunit

Interacts (when symmetrically methylated) with Papi/TDRKH (PubMed:23970546). Interacts with Vasa.

Similarity

Belongs to the argonaute family. Piwi subfamily.
Belongs to the argonaute family.

Keywords

Complete proteome   Cytoplasm   Developmental protein   Differentiation   Endonuclease   Hydrolase   Magnesium   Meiosis   Metal-binding   Methylation   Nuclease   Reference proteome   RNA-binding   RNA-mediated gene silencing   Spermatogenesis  

Feature

chain  Piwi-like protein Ago3

Uniprot

A9ZSZ2   A0A2A4J180   A0A0N1IAN2   A0A194QIA0   A0A2W1BMN2  

EC Number

3.1.26.-

Pubmed

18191035    19121390    19460866    24757166    23970546    25558067    + More
26354079    28756777   

EMBL

AB332312    AB372007    BABH01012993    NWSH01004274    PCG65284.1    KQ460317    + More
KPJ16015.1    KQ458756    KPJ05288.1    KZ150022    PZC74874.1   

Proteomes

UP000005204    UP000218220    UP000053240    UP000053268   

PRIDE

A9ZSZ2   

Pfam

PF02170   PAZ        + More
PF02171   Piwi
PF08712   Nfu_N
PF01106   NifU

Interpro

IPR012337   RNaseH-like_sf        + More
IPR003165   Piwi       
IPR003100   PAZ_dom       
IPR036397   RNaseH_sf       
IPR036085   PAZ_dom_sf       
IPR001075   NIF_FeS_clus_asmbl_NifU_C       
IPR014824   Nfu/NifU_N       
IPR034904   FSCA_dom_sf       
IPR036498   Nfu/NifU_N_sf       

SUPFAM

SSF53098   SSF53098        + More
SSF101690   SSF101690       
SSF117916   SSF117916       
SSF110836   SSF110836       

Gene 3D

3.30.420.10     3.30.1370.70     3.30.300.130    

ProteinModelPortal

A9ZSZ2   A0A2A4J180   A0A0N1IAN2   A0A194QIA0   A0A2W1BMN2  

KEGG

100125337    K02156 aubergine | (RefSeq) Ago3, BmAGO3, Piwi, Siwi2; argonaute 3

PATHWAY

04320    Dorso-ventral axis formation - Bombyx mori (domestic silkworm)

GO

GO:0051321   GO:0007275   GO:0005737   GO:0004519   GO:0046872   GO:0003723   GO:0030154   GO:0007283   GO:0031047   GO:0003676   GO:0016226   GO:0051536   GO:0005506  

Subcellular location

Cytoplasm   Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis.   With evidence from 8 publications.

Length:

109

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.02049

Exp number, first 60 AAs:

0.00474

Total prob of N-in:

0.27806

outside

1  -  109

 

 

Pi

240.562262

Theta

203.253914

Tajima's D

0.67632

CLR

0.194082

CSRT

0.566471676416179

Interpretation

Uncertain