Gene

KWMTBOMO01224

Pre Gene Modal

BGIBMGA009037

Annotation

argonaute_3_[Bombyx_mori]

Location

Bomo_Chr3(-):5532148-5537797      View in EpiBrowser

Full name

Piwi-like protein Ago3      

Location in the cell

Cytoplasmic   Reliability : 1.814 Nuclear   Reliability : 2.112

CDS

ATGTCTGAAACTTCAGTTTCAGCACAGACATCTTCAGTTGCCAAGAACAAATATTTCAGGGAGGTGAAAGACACTCCTCCAGTCGTAAAGAAAGGTGAAACAGGTGTGCCTATAGAAGTGACTTGTAACTACATTTATCTCAATTTCAAAGAGAACATTGTTTTTGAGTATGAAGTGAAGTTTGAACCAGATCAAGACTATAAACATTTGCGATTCAAATTGCTCAACGAGCACATTGAACACTTTAAGGAGAAAACATTCGATGGTACCACCCTCTACGTGCCACACGAACTGCCCGATGCAGTCCGTAATTTAGTATCTACTAACCCATATGACCAAAGCAAAGTAAACGTTTCTATTATATTTCGTCGCACACGTCGTTTGAGCGAAATGATTCACATATACAATGTGATGTTCAAATGTATAATGAAGGATTTGAAATTGATTCGCTTCGGACGCCAACATTACAATGAGCACGCAGCCATACAGATACCTCAGCATAAATTAGAAGTTTGGCCTGGATACGTAACAGCAGTAGACGAATACGAAGGCGGTCTGATGTTAACATTAGATTCGACTCATCGTGTATTACGAACGCAAACCGTTCTCTCTCTGATCAAAGAGGTTGTGCAAACTGAAGGAGCTAATTGGAAACGTAAGATGACAGATATACTAATAGGAGCCTCTGTTATGACTACTTATAACAAGAAATTGTTTAGAGTTGATACAATTGACGATAAAATGAGTCCGAGATCAACATTTGAGAAAACAGAGAAAGGCGAAACTGTACAGATTAGTTTTATAGATTATTATAAGAAGAATTATGGTATCGAGATAATGGACTGGGATCAACCCTTACTCATTTCAAGAGACACAAAACGTATGCCCGGATCAGACACTCCAACAGATTTCATGATATGCCTCATTCCGGAGTTATGTCAATTGACTGGTTTAACAGATGATCAACGCAGTAACTTTAGATTAATGAAAGATGTAGCTACTTACACGAGGATCACTCCAAATCAGCGCCATGCCGCTTTTAAGAAGTACATAGAAAGTGTAATGAAAAACGAAACTGCCAAGAGTCGTCTAGCGGGTTGGGGTCTCAGTATAGCACCGGAAACTGTGAACCTAACAGCTCGTACCCTGCCACCAGAGACACTGTATTTTGGAGACAATGTTAGAGTTCCCGAGGGATAA

Protein

MSETSVSAQTSSVAKNKYFREVKDTPPVVKKGETGVPIEVTCNYIYLNFKENIVFEYEVKFEPDQDYKHLRFKLLNEHIEHFKEKTFDGTTLYVPHELPDAVRNLVSTNPYDQSKVNVSIIFRRTRRLSEMIHIYNVMFKCIMKDLKLIRFGRQHYNEHAAIQIPQHKLEVWPGYVTAVDEYEGGLMLTLDSTHRVLRTQTVLSLIKEVVQTEGANWKRKMTDILIGASVMTTYNKKLFRVDTIDDKMSPRSTFEKTEKGETVQISFIDYYKKNYGIEIMDWDQPLLISRDTKRMPGSDTPTDFMICLIPELCQLTGLTDDQRSNFRLMKDVATYTRITPNQRHAAFKKYIESVMKNETAKSRLAGWGLSIAPETVNLTARTLPPETLYFGDNVRVPEG

Description

Endoribonuclease that plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity (PubMed:19460866). Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells (PubMed:19460866, PubMed:25558067). Its presence in oocytes suggests that it may participate in similar functions during oogenesis in females (PubMed:18191035). Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons (PubMed:19460866, PubMed:25558067). Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements (PubMed:19460866, PubMed:25558067). Strongly prefers a have adenine at position 10 of their guide (g10A preference) (PubMed:24757166, PubMed:25558067). Plays a key role in the piRNA amplification loop, also named ping-pong amplification cycle: antisense piRNA-bound Siwi and sense piRNA-bound Ago3 reciprocally cleave complementary transcripts, to couple the amplification of piRNAs with the repression of transposable elements (PubMed:25558067).

Cofactor

Mg(2+)

Subunit

Interacts (when symmetrically methylated) with Papi/TDRKH (PubMed:23970546). Interacts with Vasa.

Similarity

Belongs to the argonaute family. Piwi subfamily.
Belongs to the argonaute family.
Belongs to the peptidase S1 family.

Keywords

Complete proteome   Cytoplasm   Developmental protein   Differentiation   Endonuclease   Hydrolase   Magnesium   Meiosis   Metal-binding   Methylation   Nuclease   Reference proteome   RNA-binding   RNA-mediated gene silencing   Spermatogenesis  

Feature

chain  Piwi-like protein Ago3

Uniprot

A9ZSZ2   A0A194QIA0   A0A2R3SZW5   A0A2A4J180   A0A2W1BMN2   A0A2J7QHJ0   + More
A0A067R2W4   S4W5D9   A0A1L7NZM5   D2A2S9   A0A126G926   A0A0T6AZW1   A0A0B5FZ79   U5ER25   A0A023EVW0   A0A182HBG7   A0A182HBG4   A0A1Q3G100   A0A1Q3G0Z9   A0A140AQC8   B0WE96   Q170R5   A0A151XAQ9   E2AFE7   A0A084VYK5   A0A195ERG9   A0A158P1R9   A0A195CLN2   A0A0P5S6L6   A0A1A9WEW9   A0A232F280   A0A182JH63   A0A0P4WI04   A0A0P5QH29   A0A0P6DU52   A0A182QHG7   A0A0P5J8H2   A0A0P5JBQ8   F4W6N0   V3ZNA5   A0A0P5FLU4   A0A0P5YY47   A0A2S1PRV1   A0A182I747   A0A0P4YZB4   T1PJ36   A0A1I8JVL5   K7ISX8   A0A0P4YRN5   A0A182K9L7   T1I9T3   A0A0P4YPF0   A0A1B6BXC6   A0A1B6EE87   A0A182TQQ4   A0A182MGG0   A0A1S3JM11   Q7PWT1   A0A195BR75   A0A1Y1KFT6   A0A1I8QFA5   T2M9F7   U5XHW4   A0A1B0GAL6   A0A0L0BY98   A0A182VBS2   A0A126G8W7   A0A182VU78   A0A0V0G8P6   A0A336M4G2   A0A0P6CV87   A0A182SUB5   A0A182P6A3   A0A1A9VJA5   A0A1B6GWF8   A0A0P4WMX6   A0A076V2J9   T2HRQ9   A0A1J1IPY0   A0A182VU77   A0A1A9YD67   X2CS76   A0A023F0H9   A0A182Y956   A0A182NPY6   E2B9V7   A0A182R493   A0A224X6L1   E9GFR1   A0A0K2TEK4   M9T4E3   A0A0M5IYT6   A0A0A1WX90   A0A1B6K3S4   A0A1A9ZQW1  

EC Number

3.1.26.-

Pubmed

18191035    19121390    19460866    24757166    23970546    25558067    + More
26354079    28756777    24845553    23792802    28034629    18362917    19820115    26868596    25541004    24945155    26483478    17510324    20798317    24438588    21347285    28648823    21719571    23254933    25315136    20075255    12364791    14747013    17210077    28004739    24065732    24367095    26108605    24947897    24211384    25474469    25244985    21292972    23777831    25830018   

EMBL

AB332312    AB372007    BABH01012993    KQ458756    KPJ05288.1    MG778697    + More
AVP80830.1    NWSH01004274    PCG65284.1    KZ150022    PZC74874.1    NEVH01013973    PNF28067.1    KK853043    KDR12118.1    KF032644    AGO85969.1    LC194147    BAW35369.1    KQ971339    EFA02921.1    KR012688    ALC79963.1    LJIG01016388    KRT80752.1    KP036493    AJF15706.1    GANO01003001    JAB56870.1    GAPW01000240    JAC13358.1    JXUM01032518    KQ560958    KXJ80216.1    JXUM01032513    JXUM01032514    GFDL01001570    JAV33475.1    GFDL01001571    JAV33474.1    KR012686    ALC79961.1    DS231906    EDS45390.1    CH477469    EAT40454.1    KQ982335    KYQ57456.1    GL439108    EFN67840.1    ATLV01018376    KE525231    KFB43049.1    KQ982021    KYN30497.1    ADTU01006772    KQ977595    KYN01613.1    GDIQ01091785    JAL59941.1    NNAY01001164    OXU24926.1    GDIP01255014    JAI68387.1    GDIQ01135376    JAL16350.1    GDIQ01238138    GDIQ01072934    GDIQ01051866    JAN21803.1    AXCN02002090    GDIQ01209588    JAK42137.1    GDIQ01247184    GDIQ01206938    GDIQ01201905    GDIQ01098733    JAK49820.1    GL887707    EGI70106.1    KB203566    ESO83925.1    GDIQ01255968    JAJ95756.1    GDIP01064896    JAM38819.1    MF288051    AWH61368.1    APCN01003364    GDIP01224717    JAI98684.1    KA648130    AFP62759.1    AAZX01006478    GDIP01224716    JAI98685.1    ACPB03019172    GDIP01224715    JAI98686.1    GEDC01031380    GEDC01013016    JAS05918.1    JAS24282.1    GEDC01023472    GEDC01019529    GEDC01016336    GEDC01001067    JAS13826.1    JAS17769.1    JAS20962.1    JAS36231.1    AXCM01010884    AAAB01008984    EAA14901.5    KQ976419    KYM89356.1    GEZM01087790    JAV58475.1    HAAD01002308    CDG68540.1    KF411462    AGZ62175.1    CCAG010011279    JRES01001160    KNC24995.1    KR012687    ALC79962.1    GECL01001984    JAP04140.1    UFQT01000328    SSX23287.1    GDIQ01088032    JAN06705.1    GECZ01003022    JAS66747.1    GDRN01060272    JAI65395.1    KJ808823    AIK19232.1    AB840993    BAN82534.1    CVRI01000055    CRL01596.1    KC203335    AGV15455.1    GBBI01003735    JAC14977.1    GL446605    EFN87544.1    GFTR01008350    JAW08076.1    GL732542    EFX81763.1    HACA01006510    CDW23871.1    KC516710    AGI95997.1    CP012523    ALC39198.1    GBXI01010825    JAD03467.1    GECU01001606    JAT06101.1   

Proteomes

UP000005204    UP000053268    UP000218220    UP000235965    UP000027135    UP000007266    + More
UP000192223    UP000069940    UP000249989    UP000002320    UP000008820    UP000075809    UP000000311    UP000030765    UP000078541    UP000005205    UP000078542    UP000091820    UP000215335    UP000075880    UP000075886    UP000007755    UP000030746    UP000075840    UP000095301    UP000076407    UP000002358    UP000075881    UP000015103    UP000075902    UP000075883    UP000085678    UP000007062    UP000078540    UP000095300    UP000092444    UP000037069    UP000075903    UP000075920    UP000075901    UP000075885    UP000078200    UP000183832    UP000092443    UP000076408    UP000075884    UP000008237    UP000075900    UP000000305    UP000092553    UP000092445   

PRIDE

A9ZSZ2   

Pfam

PF02170   PAZ        + More
PF02171   Piwi
PF08699   ArgoL1
PF00089   Trypsin
PF10230   LIDHydrolase
PF04153   NOT2_3_5

Interpro

IPR012337   RNaseH-like_sf        + More
IPR003165   Piwi       
IPR003100   PAZ_dom       
IPR036397   RNaseH_sf       
IPR036085   PAZ_dom_sf       
IPR014811   ArgoL1       
IPR033116   TRYPSIN_SER       
IPR009003   Peptidase_S1_PA       
IPR001314   Peptidase_S1A       
IPR018114   TRYPSIN_HIS       
IPR001254   Trypsin_dom       
IPR029058   AB_hydrolase       
IPR019363   LDAH       
IPR007282   NOT2/3/5_C       
IPR038635   CCR4-NOT_su2/3/5_N_sf       

SUPFAM

SSF53098   SSF53098        + More
SSF101690   SSF101690       
SSF50494   SSF50494       
SSF53474   SSF53474       

Gene 3D

3.30.420.10     3.40.50.1820     2.30.30.1020    

CDD

cd00190  Tryp_SPc

ProteinModelPortal

A9ZSZ2   A0A194QIA0   A0A2R3SZW5   A0A2A4J180   A0A2W1BMN2   A0A2J7QHJ0   + More
A0A067R2W4   S4W5D9   A0A1L7NZM5   D2A2S9   A0A126G926   A0A0T6AZW1   A0A0B5FZ79   U5ER25   A0A023EVW0   A0A182HBG7   A0A182HBG4   A0A1Q3G100   A0A1Q3G0Z9   A0A140AQC8   B0WE96   Q170R5   A0A151XAQ9   E2AFE7   A0A084VYK5   A0A195ERG9   A0A158P1R9   A0A195CLN2   A0A0P5S6L6   A0A1A9WEW9   A0A232F280   A0A182JH63   A0A0P4WI04   A0A0P5QH29   A0A0P6DU52   A0A182QHG7   A0A0P5J8H2   A0A0P5JBQ8   F4W6N0   V3ZNA5   A0A0P5FLU4   A0A0P5YY47   A0A2S1PRV1   A0A182I747   A0A0P4YZB4   T1PJ36   A0A1I8JVL5   K7ISX8   A0A0P4YRN5   A0A182K9L7   T1I9T3   A0A0P4YPF0   A0A1B6BXC6   A0A1B6EE87   A0A182TQQ4   A0A182MGG0   A0A1S3JM11   Q7PWT1   A0A195BR75   A0A1Y1KFT6   A0A1I8QFA5   T2M9F7   U5XHW4   A0A1B0GAL6   A0A0L0BY98   A0A182VBS2   A0A126G8W7   A0A182VU78   A0A0V0G8P6   A0A336M4G2   A0A0P6CV87   A0A182SUB5   A0A182P6A3   A0A1A9VJA5   A0A1B6GWF8   A0A0P4WMX6   A0A076V2J9   T2HRQ9   A0A1J1IPY0   A0A182VU77   A0A1A9YD67   X2CS76   A0A023F0H9   A0A182Y956   A0A182NPY6   E2B9V7   A0A182R493   A0A224X6L1   E9GFR1   A0A0K2TEK4   M9T4E3   A0A0M5IYT6   A0A0A1WX90   A0A1B6K3S4   A0A1A9ZQW1  

PDB

5GUH     E-value=5.66227e-60,     Score=585

KEGG

100125337    K02156 aubergine | (RefSeq) Ago3, BmAGO3, Piwi, Siwi2; argonaute 3

GO

GO:0051321   GO:0007275   GO:0005737   GO:0004519   GO:0046872   GO:0003723   GO:0030154   GO:0007283   GO:0031047   GO:0003676   GO:0003743   GO:0004252   GO:0005634   GO:0006355   GO:0005515   GO:0004497   GO:0005507   GO:0006000   GO:0004553   GO:0006334   GO:0003779   GO:0016021  

Subcellular location

Cytoplasm   Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis.   With evidence from 8 publications.

Length:

399

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.00684999999999999

Exp number, first 60 AAs:

0.00015

Total prob of N-in:

0.01298

outside

1  -  399

 

 

Pi

199.666989

Theta

163.503462

Tajima's D

0.856178

CLR

0.323008

CSRT

0.625618719064047

Interpretation

Uncertain