SGID Silkworm Genome Informatics Database
Gene
KWMTBOMO01001  Validated by peptides from experiments
Pre Gene Modal
BGIBMGA001347
Annotation
fibroin_P25_[Bombyx_mandarina]
Location
Bomo_Chr2(-):8224287-8227421      View in EpiBrowser
Full name
Fibrohexamerin      
Alternative Name
25 kDa silk glycoprotein
p25
Location in the cell
Extracellular   Reliability : 2.257 PlasmaMembrane   Reliability : 1.766
 

Sequence

CDS
ATGCTGGCGCGGTGTCTAGCTGTAGCCGCTGTGGCAGTTTTGGCTTCTGCAGGGCCGCCGAGTCCGATCTACAGGCCCTGCTACTTGGACGATTACAAATGCATCTCCGACCACCTGGCCGCGATCTCGAAATGTATCCCGGGCAGAGGGCAGATCCCCTCGCAGTACGAGATACCGGTGTTCCAGTTTGAAATACCATACTTCAACGCCACCTACGTCGACCATAATCTCATTACCCGTAACCACGACAAATGCCGTGTCAGCGAATTTTATGACAATGTGAGAACTTTAAAAACAGTTTTAACCGTGGACTGTCCGTGGCTGAACTTTGAATCCAATCGGACCTTGGCTCAGCACATGTCGTTCAAGGAAGACGTCGTCTTAAGCTTCTACATCAATGGATCTTATCCTTTGATCAGGCTAACGACAGTGTTTGATAAGGGCAACAATTTCGACTTGTGTTCGGCTTTCACGTTCGCAGACCTGGCCGGGGGTCTGCCCATCTTCCACATTAACCCTAATGACCAACGCACAGCGCAGTGGTTGTCTAAAGATCTGACCCTGCTGCACATTTACGAGAGAGAGCACATCTTCGGAAAGAGGAACTGGCTGGCGAGGTCCTTCATCAGCAGAACACTCTGCGACTTCGGTTGTCACCACTGA
Protein
MLARCLAVAAVAVLASAGPPSPIYRPCYLDDYKCISDHLAAISKCIPGRGQIPSQYEIPVFQFEIPYFNATYVDHNLITRNHDKCRVSEFYDNVRTLKTVLTVDCPWLNFESNRTLAQHMSFKEDVVLSFYINGSYPLIRLTTVFDKGNNFDLCSAFTFADLAGGLPIFHINPNDQRTAQWLSKDLTLLHIYEREHIFGKRNWLARSFISRTLCDFGCHH

Summary

Subunit
Silk fibroin elementary unit consists in a disulfide-linked heavy and light chain and a p25 glycoprotein in molar ratios of 6:6:1. This results in a complex of approximately 2.3 MDa.
Miscellaneous
It is unclear whether the N-terminal residue of the mature protein is Ala-17 or Gly-18.
Keywords
Complete proteome   Direct protein sequencing   Disulfide bond   Glycoprotein   Reference proteome   Secreted   Signal   Silk protein  
Feature
chain  Fibrohexamerin
Uniprot
H9IVR6   Q9BLL8   P04148   A0A212EPK5   O62605   D0EWD9   + More
Q9BLL6   A0A194PTN4   Q9BLL4   A0A0N1IP14   A0A2A4JH46   A0A1V0PND8   Q14UU6   A0A2H1VQJ6   A0A0L7LN50   A0A2A4J112   A0A2W1BYP1   A0A0L7L1X1   A0A2A4J2D9   H9JIA9   A0A2A4JDL7   A0A0L7L2A8   A0A2W1BSA2   A0A0M3VH85   A0A0M4UR85  
Pubmed
19121390    11267905    3748814    14614147    15141943    2987837    + More
10986287    22118469    9520260    9583947    7715595    20171223    26354079    16755355    26227816    28756777   
EMBL
BABH01038094    BABH01038095    AB001821    BAB39500.1    X04226    BP122961    + More
BP126317    BP177743    BAAB01057839    AGBW02013452    OWR43406.1    AF009827    AF009677    GQ901976    ACX50393.1    AB001823    BAB39502.1    KQ459593    KPI96677.1    AB001825    BAB39504.1    KQ460854    KPJ11963.1    NWSH01001474    PCG71139.1    KY792994    ARE31005.1    AB195976    BAE97692.1    ODYU01003822    SOQ43056.1    JTDY01000571    KOB76636.1    NWSH01003760    PCG65847.1    KZ149916    PZC77940.1    JTDY01003497    KOB69467.1    PCG65848.1    BABH01022225    NWSH01001990    PCG69503.1    KOB69466.1    PZC77938.1    LC001864    BAS31052.1    LC001863    BAS31051.1   
Proteomes
UP000005204    UP000007151    UP000053268    UP000053240    UP000218220    UP000037510    + More
PRIDE
H9IVR6    Q9BLL8    P04148    Q9BLL6    Q9BLL4    H9JIA9    + More
Pfam
PF07294   Fibroin_P25
Interpro
IPR009911   Fibroin_P25        + More
IPR016024   ARM-type_fold       
IPR011989   ARM-like       
IPR038606   To_sf       
SUPFAM
SSF48371   SSF48371       
Gene 3D
1.25.10.10     3.15.10.30    
ProteinModelPortal
H9IVR6   Q9BLL8   P04148   A0A212EPK5   O62605   D0EWD9   + More
Q9BLL6   A0A194PTN4   Q9BLL4   A0A0N1IP14   A0A2A4JH46   A0A1V0PND8   Q14UU6   A0A2H1VQJ6   A0A0L7LN50   A0A2A4J112   A0A2W1BYP1   A0A0L7L1X1   A0A2A4J2D9   H9JIA9   A0A2A4JDL7   A0A0L7L2A8   A0A2W1BSA2   A0A0M3VH85   A0A0M4UR85  

Ontologies

KEGG
100146105    no KO assigned | (RefSeq) P25; silk protein P25
GO
GO:0005576   GO:0005198   GO:0016020   GO:0005925   GO:0007016   GO:0005856   GO:0007155   GO:0051015   GO:0003779   GO:0016021  

Topology

Subcellular location
Secreted  
SignalP
Position:   1 - 17,         Likelihood:  0.997161
 
 
Length:
220
Number of predicted TMHs:
0
Exp number of AAs in TMHs:
1.63405
Exp number, first 60 AAs:
1.45424
Total prob of N-in:
0.12829
outside
1  -  220
 
 

Population Genetic Test Statistics

Pi
288.442107
Theta
210.220679
Tajima's D
0.910725
CLR
0.737083
CSRT
0.634218289085546
Interpretation
Uncertain
Peptides ×
Source Sequence Identity Evalue
20029844 LTTVFDKGNNFDLCSAF 100.00 9e-16
20029844 TLAQHMSFK 100.00 9e-16
20029844 EDVVLSFYIN*GSYPLIR 96.77 5e-14
20029844 EDVVLSFYIN*GSYPLIR 96.77 5e-14
20029844 EDVVLSFYIN*GSYPLIR 96.77 5e-14
20029844 GNNFDLCSAFTFADLAGGLPIFHINPNDQR 96.77 5e-14
20029844 EDVVLSFYIN*GSYPLIR 96.77 5e-14
20029844 TFADLAGGLPIFHINPNDQRTAQ 100.00 1e-12
20029844 TFADLAGGLPIFHINPNDQRTAQ 100.00 1e-12
20029844 DLCSAFTFA 100.00 1e-12
20029844 DLCSAFTFA 100.00 1e-12
20029844 DLAGGLPIFHINPN 100.00 1e-12
20029844 TFADLAGGLPIFHINPNDQRTAQW 100.00 3e-11
20029844 TFADLAGGLPIFHINPNDQRTAQ 100.00 3e-11
20029844 ADLAGGLPIFH 100.00 3e-11
20029844 TFADLAGGLPIFHINPNDQ*RTAQW 100.00 3e-11
20029844 TFADLAGGLPIFH 100.00 5e-11
20029844 TFAD*LAGGLPIFH 100.00 5e-11
20029844 TFADLAGGLPIFH 100.00 5e-11
20029844 TFADLAGGLPIF 100.00 5e-11
20029844 DKGN*NFDLCSAF 100.00 5e-11
20029844 TFADLAGGLPIFHINPNDQRTAQ 96.00 8e-11
20029844 TFADLAGGLPIFHINPNDQRTAQ 96.00 8e-11
20029844 TFADLAGGLPIFH 96.00 8e-11
20029844 ADLAGGLPIFHINPNDQRTA 95.83 3e-10
20029844 ADLAGGLPIFHINPNDQRTA 95.83 3e-10
20029844 GPPSPIYRPCYLDD*Y 100.00 4e-10
20029844 GPPSPIYRPCYLDD*Y 100.00 4e-10
20029844 ADLAGGLPIFH 95.65 2e-09
20029844 YLDDYKCISDHL 100.00 2e-09
20029844 ADLAGGLPIFHINPNDQRTAQW 100.00 5e-09
20029844 DLCSAFTFA 100.00 6e-09
20029844 TTVFDKGNNFDLC 100.00 7e-09
20029844 TVFDKGNNFDLC 100.00 7e-09
20029844 ADLAGGLPIFHINPN 100.00 7e-09
20029844 TTVFDKGNNFDLC 100.00 7e-09
20029844 ADLAGGLPIFHINPN 100.00 7e-09
20029844 ADLAGGLPIFH 100.00 7e-09
20029844 GNNFDLCSAFTFADLAGGLPIFHI*NPNDQR 100.00 3e-08
20029844 GNNFDLCSAFTFADLAGGLPIFHINPNDQR 100.00 3e-08
20029844 YLDDYKCISDHL 100.00 7e-08
20029844 YLDDYKCISDHL 100.00 7e-08
20029844 SKDLTLL 100.00 8e-08
20029844 AGPPSPIYRPCYLD 100.00 8e-08
20029844 SYPLIRLTTVF 100.00 3e-07
20029844 ADLAGGLPIFHINPNDQRTA 95.24 4e-07
20029844 DHLAAN*SKCIPGRGQIPSQY 100.00 2e-06
20029844 GPPSPIYRPCYLD 100.00 2e-06
20029844 DHLAAN*SKCIPGRGQIPSQY 100.00 2e-06
20029844 INGSYPLIRL 100.00 2e-06
20029844 EDVVLSFYIN*GSYPLIR 100.00 1e-05
20029844 DLTLLHIY 100.00 1e-05
20029844 SITDLLRGV 100.00 1e-05
20029844 DLAGGLPIFH 100.00 2e-05
20029844 GGLPIFHINPNDQ 100.00 2e-05
20029844 GGLPIFHINPNDQ 100.00 2e-05
20029844 GGLPIFHINPNDQR 100.00 2e-05
20029844 ADLAGGLPIFHINPN 100.00 2e-05
20029844 GGLPIFHIN*PN 100.00 2e-05
31253818 Carbamidomethyl 100.00 2e-05
20029844 DLTLLHIYER 100.00 2e-05
20029844 DLTLLHIYER 100.00 2e-05
20029844 LSKDLTLLHIY 100.00 4e-05
20029844 ADLAGGLPIFHINPNDQRTAQW 100.00 4e-05
20029844 IRLTTVF 100.00 4e-05
20029844 IRLTTVF 100.00 4e-05
20029844 IN*GSYPLIRLTTVFDKGNNF 100.00 4e-05
20029844 IRLTTVFDKGNNFDLCSAF 100.00 4e-05
20029844 ADLAGGLPIFH 100.00 5e-05
20029844 ADLAGGLPIFH 100.00 5e-05
20029844 TTVFDKGNNFDLC 100.00 5e-05
20029844 GPPSPIYRPCYLDDYKCISDHL 100.00 5e-05
20029844 GPPSPIYRPCYLDD*Y 100.00 5e-05
20029844 TLCDFGCHH 100.00 6e-05
20029844 GPPSPIYRPC 100.00 8e-05
20029844 DLTLLHIYER 100.00 8e-05
20029844 GPPSPIYRPC 100.00 8e-05
20029844 AGPPSPIYRPCYLDDY 100.00 8e-05
20029844 TLCDFGCHH 100.00 8e-05
20029844 RNWLAR 100.00 8e-05
20029844 VSEFYDNVR 100.00 1e-04
20029844 VSEFYDNVR 100.00 1e-04
20029844 TVLTVDCPWLNFESN*R 100.00 1e-04
20029844 VSEFYDNVR 100.00 1e-04
20029844 TVLTVDCPWLNFESN*R 100.00 1e-04
20029844 VSEFYDNVR 100.00 1e-04
20029844 VSEFYDNVR 100.00 1e-04
20029844 TVLTVDCPWLNFESN*R 100.00 1e-04
20029844 VSEFYDNVR 100.00 1e-04
20029844 TTVFDKGNNFDLCSAF 100.00 2e-04
20029844 IRLTTVFDKGNN*FDLCSAF 100.00 2e-04
20029844 DLCSAFTFA 100.00 3e-04
20029844 DLCSAFTFA 100.00 3e-04
20029844 DLCSAFTFA 100.00 3e-04
20029844 GPPSPIYRPCYLDDY 100.00 3e-04
20029844 GGLPIFHINPNDQ 100.00 4e-04
20029844 GPPSPIYRPCYLDDY 100.00 4e-04
20029844 GPPSPIYRPCYLDDY 100.00 4e-04
20029844 GPPSPIYRPCYLDDY 100.00 4e-04
20029844 GPPSPIYRPCYLDDY 100.00 4e-04
20029844 KTVLTVDCPW 100.00 0.001
20029844 KTVLTVDCPW* 100.00 0.001
20029844 KTVLTVDCPW* 100.00 0.001
20029844 KTVLTVDCPW* 100.00 0.001
20029844 KTVLTVDCPW 100.00 0.001
20029844 DQCRVSEFYDNVRTL 100.00 0.001
20029844 GQIPSQYEIPVFR 100.00 0.001
20029844 CISDHLAAN*SK 100.00 0.001
20029844 CISDHLAAN*SK 100.00 0.001
20029844 CISDHLAAN*SK 100.00 0.001
20029844 CISDHLAAN*SK 100.00 0.001
20029844 CISDHLAAN*SK 100.00 0.001
20029844 GGLPIFHINPNDQ*RTAQWL 100.00 0.002
20029844 HIYEREHIFGKR 100.00 0.002
20029844 AAN*SKCIPGRGQIPSQY 100.00 0.002
20029844 DKGNNFDLCSAF 100.00 0.002
20029844 DKGNNFDLCSAF 100.00 0.002
20029844 DKGNNFDLCSAF 100.00 0.002
20029844 TTVFDKGNN*FDLCSAF 100.00 0.002
20029844 DKGNN*FDLCSAF 100.00 0.002
20029844 HIYEREHIFGKR 100.00 0.002
20029844 AGPPSPIYRPCYL 100.00 0.002
20029844 AGPPSPIYRPCYL 100.00 0.002
20029844 SKDLTLL 100.00 0.003
20029844 SKDLTLL 100.00 0.003
20029844 GGLPIFHINPN 100.00 0.003
20029844 DLAGGLPIFH 100.00 0.003
20029844 AGGLPIFHINPN 100.00 0.003
20029844 GGLPIFHINPN 100.00 0.003
20029844 YLDDYKCISDHL 100.00 0.004
20029844 EFYDNVRTL 100.00 0.004
20029844 EFYDNVRTL 100.00 0.004
20029844 INGSYPLIRL 100.00 0.004
20029844 ADLAGGLPIFHINPNDQRTA 100.00 0.006
20029844 ADLAGGLPIFHINPN*DQRTAQW 100.00 0.007
20029844 SKDLTLL 100.00 0.007
20029844 ADLAGGLPIFHINPNDQ*RTAQWL 100.00 0.007
20029844 SFKEDVVLS 100.00 0.016
20029844 SFKEDVVL 100.00 0.016
20029844 YINGSYPL 100.00 0.016
20029844 SFKEDVVLS 100.00 0.016
20029844 SFKEDVVL 100.00 0.016
20029844 SFKEDVVLS 100.00 0.016
20029844 TTVFDKGNNFDLCSAF 100.00 0.017
20029844 TTVFDKGNNFDLCSAF 100.00 0.017
20029844 TVFDKGNNFDLCSAF 100.00 0.017
20029844 TTVFDKGN*NFDLCSAF 100.00 0.017
20029844 GPPSPIYRPC 100.00 0.039
20029844 GPPSPIYRPC 100.00 0.039
20029844 GPPSPIYRPC 100.00 0.039
20029844 GPPSPIYRPC 100.00 0.039
20029844 GPPSPIYRPC 100.00 0.039
20029844 GPPSPIYRPC 100.00 0.039
20029844 YLDDYKCI 100.00 0.039
20029844 YLDDYKCI 100.00 0.039
20029844 GGLPIFHINPNDQ 100.00 0.039
20029844 GPPSPIYRPC 100.00 0.039
20029844 GPPSPIYRPC 100.00 0.039
20029844 GPPSPIYRPC 100.00 0.039
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