Gene

KWMTBOMO00995  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA001348

Annotation

PREDICTED:_aldo-keto_reductase_AKR2E4-like_[Amyelois_transitella]

Location

Bomo_Chr2(-):8165688-8172891      View in EpiBrowser

Full name

Aldo-keto reductase AKR2E4      

Alternative Name

3-dehydroecdysone reductase
Aldo-keto reductase 2E

Location in the cell

Cytoplasmic   Reliability : 4.149

CDS

ATGGAAGTGCCCTCGTTAAAAATGAACAACGGCAGAGACATGCCCGCCATCGGCCTGGGCACGTACCTTGGGTTTGATCAGAACGGCGTAGTGAAGTCCAAGGACAAGCAGCTCCGTGATGTTGTAATGCGCGCCATAGATGTCGGATACCGTCATTTCGATACCGCCTCCGTTTACGAAACTGAACAAGAAATTGGAGAAGCCGTGAAGATGAAGGTAGAGGAAGGAGTCGTCAAAAGAGAAGATATCTTTATCACTACTAAGCTGTGGAATACCCAGCACCGGAGAACTGAAGTAGCCCAAGCTATAAACGATTCACTGAAGAAACTAGGTCTGGATTATATAGATCTTTATTTGATGCACTGGCCCATTGGATTAAATGCCGATTATAGTCATTCGGACGTAGACTTCATGGAGACCTGGCGAGGATTGGAAGATGCTCAGCGAATGGGTATAGTTAAAGGCATCGGACTGTCTAACTTCAACAAGCAGCAGTTGGAGAGGGTGCTGAAGGAAGGCACTATCAAACCTGATGCTATTCAAATAGAGGTTCACCTCCAAAACGTTCAGAAAGAAATGGTGGAATTTTGTCAGTCTGAAGGTGTTGTAGTCATGGGCTATTCTCCTTTCGGTTCCTTGGTAGCCAGACATGGTTCGACAGTCGAAGGGCCCAAGATCGACGATCCAGTGCTCAGCTCCATCGCCCAGAAATACGGGAAGACCACTCCTCAAATTGTCTTGAGATGGCAGATTGAGCGCAAAGTCGTGCCAATCCCCAAAACCGTCAACCCGAAACGCCTGGAGGAGAACATTAACATCTTCGACTTCACTCTTACACCAGACGAGGTCGAGAAGATTAACGGGTTCGATACACATACCAGATACACCCTGCCGTCGTTTTGGCAGAACCACCCGTATTACCCGTTCGACAAGGTTGATAATCCCATCGCTGACCCTTTTATTAAGGTATAG

Protein

MEVPSLKMNNGRDMPAIGLGTYLGFDQNGVVKSKDKQLRDVVMRAIDVGYRHFDTASVYETEQEIGEAVKMKVEEGVVKREDIFITTKLWNTQHRRTEVAQAINDSLKKLGLDYIDLYLMHWPIGLNADYSHSDVDFMETWRGLEDAQRMGIVKGIGLSNFNKQQLERVLKEGTIKPDAIQIEVHLQNVQKEMVEFCQSEGVVVMGYSPFGSLVARHGSTVEGPKIDDPVLSSIAQKYGKTTPQIVLRWQIERKVVPIPKTVNPKRLEENINIFDFTLTPDEVEKINGFDTHTRYTLPSFWQNHPYYPFDKVDNPIADPFIKV

Description

NADP-dependent oxidoreductase with high 3-dehydroecdysone reductase activity. May play a role in the regulation of molting. Has lower activity with phenylglyoxal and isatin (in vitro). Has no activity with NADH as cosubstrate. Has no activity with nitrobenzaldehyde and 3-hydroxybenzaldehyde.

Biophysicochemical Properties

0.0044 mM for 3-dehydroecdysone

Similarity

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Keywords

3D-structure   Complete proteome   Lipid metabolism   NADP   Oxidoreductase   Reference proteome   Steroid metabolism  

Feature

chain  Aldo-keto reductase AKR2E4

Uniprot

A0A1B2G267   H9IVR7   A0A2A4IW06   A0A2A4K2R0   A0A1Z2RRK0   A0A194PUL9   + More
Q9Y020   A0A1L8D6N3   A0A194QZZ2   A0A212EPL8   H9IVT6   A0A2H1WN92   I4DKQ3   A0A1L8D6L9   A0A212ENH0   G9F9G0   S4P5J6   A0A194RJ39   A0A212FE35   A0A291P0W7   G9F9F7   I4DKC0   D2SNV7   B8ZX07   A0A291P0T7   A0A2A4JMB2   A0A2H1WKT0   A0A291P0V4   A0A291P0Y7   A0A212FML6   D2SNM4   B2DBM2   I4DK12   A0A226F456   A0A2H1WKV4   A0A2W1BLQ6   H9JTG9   G9F9F8   A0A212FE89   A0A291P0V2   I4DP16   U5KCB8   A0A194Q090   A0A194PSF7   A0A212EU63   A0A2W1BC51   H9IVS0   G9F9G1   A0A194PV62   H9JJU8   D6WCD5   A0A194R6Z8   H9JJU9   A0A212ERG2   A0A2H1VAA3   A0A291P0V1   A0A291P0Z6   A0A194QT42   A0A154PH71   A0A194QCQ5   A0A023GIA4   A0A084WAD5   A0A224YMP8   A0A0H5AQ19   D6WBY0   A0A182URC3   A0A2H1VGR3   A0A1B0A6Q4   A0A1W4WL13   A0A182YBI3   L7LZR2   A0A131YS64   A0A131XFQ9   A0A291P0S0   A0A0U4B4X4   A0A1A9UZX4   A0A2A4JDC7   A0A182WQZ8   E0VNI1   A0A212F4I6   A0A232EMX7   A0A2H1VAB6   A0A2J7QL56   A0A182SQ44   A0A067RDB7   A0A1Y1MIA1   U5KC22   A0A182SGB6   A0A1Q3FN84   A0A291P0X3   A0A182WDU2   A0A182U6B6   A0A0L7QVS4   D3TRQ9   A0A182QI37   A0A182M0L3   A0A1A9W751   A0A2M4AF69   A0A2M4BTH9  

EC Number

1.1.1.-

Pubmed

19121390    28605547    26354079    10187849    22118469    22651552    + More
23622113    28986331    20074338    18712529    28756777    24012638    24053512    18362917    19820115    27103441    24438588    28797301    25244985    25576852    26830274    28049606    26694822    20566863    28648823    24845553    28004739    20353571   

EMBL

KU233524    ANZ02995.1    BABH01038087    BABH01038088    BABH01038089    BABH01038090    + More
NWSH01006101    PCG63656.1    NWSH01000243    PCG78063.1    KY938815    ASA46456.1    KQ459593    KPI96444.1    AJ131966    CAB41997.1    GEYN01000016    JAV02113.1    KQ460890    KPJ10854.1    AGBW02013452    OWR43401.1    BABH01038068    BABH01038069    BABH01038070    ODYU01009843    SOQ54535.1    AK401871    BAM18493.1    GEYN01000020    JAV02109.1    AGBW02013667    OWR43007.1    JN033705    AEW46858.1    GAIX01005504    JAA87056.1    KQ460152    KPJ17355.1    AGBW02008974    OWR52019.1    MF687615    ATJ44541.1    JN033702    AEW46855.1    AK401738    BAM18360.1    EZ407241    ACX53798.1    FM955425    CAW30924.1    MF687576    ATJ44502.1    NWSH01001060    PCG72858.1    ODYU01009309    SOQ53638.1    MF687578    ATJ44504.1    MF687609    ATJ44535.1    AGBW02007651    OWR54929.1    EZ407153    ACX53715.1    AB264706    BAG30781.1    AK401630    BAM18252.1    LNIX01000001    OXA64214.1    SOQ53637.1    KZ150094    PZC73640.1    AB701383    JN033703    AEW46856.1    OWR52018.1    MF687611    ATJ44537.1    AK403246    BAM19656.1    KC007356    AGQ45621.1    KQ459582    KPI98997.1    KPI96371.1    AGBW02012481    OWR44997.1    KZ150230    PZC72001.1    BABH01038066    LC375687    BBD35022.1    JN033706    AEW46859.1    KPI96644.1    BABH01017849    KQ971311    EEZ99013.1    KQ460878    KPJ11641.1    BABH01017850    BABH01017851    BABH01017852    AGBW02013028    OWR44065.1    ODYU01001496    SOQ37780.1    MF687580    ATJ44506.1    MF687618    ATJ44544.1    KQ461155    KPJ08170.1    KQ434902    KZC11162.1    KQ459232    KPJ02765.1    GBBM01001779    JAC33639.1    ATLV01022099    KE525328    KFB47179.1    GFPF01005873    MAA17019.1    LC012974    LC375688    BAR91212.1    BBD35023.1    EEZ98895.1    ODYU01002492    SOQ40039.1    GACK01007739    JAA57295.1    GEDV01007816    JAP80741.1    GEFH01003399    JAP65182.1    MF687575    ATJ44501.1    KU218649    ALX00029.1    NWSH01001964    PCG69554.1    DS235339    EEB14937.1    AGBW02010353    OWR48651.1    NNAY01003257    OXU19723.1    SOQ37779.1    NEVH01013254    PNF29307.1    KK852575    KDR21029.1    GEZM01032290    JAV84688.1    KC007348    AGQ45613.1    GFDL01006014    JAV29031.1    MF687617    ATJ44543.1    KQ414726    KOC62649.1    EZ424111    ADD20387.1    AXCN02001099    AXCM01001195    GGFK01006115    MBW39436.1    GGFJ01007171    MBW56312.1   

Proteomes

UP000005204    UP000218220    UP000053268    UP000053240    UP000007151    UP000198287    + More
UP000007266    UP000076502    UP000030765    UP000075903    UP000092445    UP000192223    UP000076408    UP000078200    UP000075920    UP000009046    UP000215335    UP000235965    UP000075901    UP000027135    UP000075902    UP000053825    UP000075886    UP000075883    UP000091820   

PRIDE

H9IVR7    H9IVT6    H9JTG9    H9IVS0    H9JJU8    H9JJU9    + More
L7LZR2   

Pfam

PF00248   Aldo_ket_red        + More
PF13516   LRR_6
PF12937   F-box-like

Interpro

IPR036812   NADP_OxRdtase_dom_sf        + More
IPR020471   Aldo/keto_reductase       
IPR018170   Aldo/ket_reductase_CS       
IPR023210   NADP_OxRdtase_dom       
IPR001611   Leu-rich_rpt       
IPR001810   F-box_dom       
IPR032675   LRR_dom_sf       
IPR006553   Leu-rich_rpt_Cys-con_subtyp       
IPR011004   Trimer_LpxA-like_sf       
IPR036691   Endo/exonu/phosph_ase_sf       

SUPFAM

SSF51430   SSF51430        + More
SSF51161   SSF51161       

Gene 3D

3.20.20.100     3.80.10.10     3.60.10.10    

CDD

cd06660  Aldo_ket_red

ProteinModelPortal

A0A1B2G267   H9IVR7   A0A2A4IW06   A0A2A4K2R0   A0A1Z2RRK0   A0A194PUL9   + More
Q9Y020   A0A1L8D6N3   A0A194QZZ2   A0A212EPL8   H9IVT6   A0A2H1WN92   I4DKQ3   A0A1L8D6L9   A0A212ENH0   G9F9G0   S4P5J6   A0A194RJ39   A0A212FE35   A0A291P0W7   G9F9F7   I4DKC0   D2SNV7   B8ZX07   A0A291P0T7   A0A2A4JMB2   A0A2H1WKT0   A0A291P0V4   A0A291P0Y7   A0A212FML6   D2SNM4   B2DBM2   I4DK12   A0A226F456   A0A2H1WKV4   A0A2W1BLQ6   H9JTG9   G9F9F8   A0A212FE89   A0A291P0V2   I4DP16   U5KCB8   A0A194Q090   A0A194PSF7   A0A212EU63   A0A2W1BC51   H9IVS0   G9F9G1   A0A194PV62   H9JJU8   D6WCD5   A0A194R6Z8   H9JJU9   A0A212ERG2   A0A2H1VAA3   A0A291P0V1   A0A291P0Z6   A0A194QT42   A0A154PH71   A0A194QCQ5   A0A023GIA4   A0A084WAD5   A0A224YMP8   A0A0H5AQ19   D6WBY0   A0A182URC3   A0A2H1VGR3   A0A1B0A6Q4   A0A1W4WL13   A0A182YBI3   L7LZR2   A0A131YS64   A0A131XFQ9   A0A291P0S0   A0A0U4B4X4   A0A1A9UZX4   A0A2A4JDC7   A0A182WQZ8   E0VNI1   A0A212F4I6   A0A232EMX7   A0A2H1VAB6   A0A2J7QL56   A0A182SQ44   A0A067RDB7   A0A1Y1MIA1   U5KC22   A0A182SGB6   A0A1Q3FN84   A0A291P0X3   A0A182WDU2   A0A182U6B6   A0A0L7QVS4   D3TRQ9   A0A182QI37   A0A182M0L3   A0A1A9W751   A0A2M4AF69   A0A2M4BTH9  

PDB

3WCZ     E-value=5.64223e-79,     Score=748

KEGG

101735876    K00011 aldehyde reductase [EC:1.1.1.21] | (RefSeq) aldo-keto reductase AKR2E4-like

PATHWAY

00040    Pentose and glucuronate interconversions - Bombyx mori (domestic silkworm)
00051    Fructose and mannose metabolism - Bombyx mori (domestic silkworm)
00052    Galactose metabolism - Bombyx mori (domestic silkworm)
00561    Glycerolipid metabolism - Bombyx mori (domestic silkworm)
00790    Folate biosynthesis - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)

GO

GO:0016491   GO:0008202   GO:0005829   GO:0008106   GO:0004032   GO:0000166   GO:0032222   GO:0051015   GO:0005925   GO:0007016   GO:0005856   GO:0007155   GO:0003779   GO:0016021   GO:0055114  

PANTHER

PTHR11732  

Length:

323

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.00311

Exp number, first 60 AAs:

0.00202

Total prob of N-in:

0.01707

outside

1  -  323

 

 

Pi

22.957939

Theta

203.465952

Tajima's D

-1.855326

CLR

0.673506

CSRT

0.0248987550622469

Interpretation

Uncertain