Gene

KWMTBOMO00985  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA001367

Annotation

PREDICTED:_aldo-keto_reductase_AKR2E4-like_[Papilio_machaon]

Location

Bomo_Chr2(+):7904598-7916460      View in EpiBrowser

Full name

Aldo-keto reductase AKR2E4      

Alternative Name

3-dehydroecdysone reductase
Aldo-keto reductase 2E

Location in the cell

Cytoplasmic   Reliability : 2.133

CDS

ATGGTTGTTTACAAATATTCCTTTGTGTTCGTTTTCGCTGCTGTCATCCATCTATCTCCATCCGCAGGATTAGCCCCAGTTCTTAAGTTGAACAATGGCAAGGAAATTCCGGGCTTCGGCATCGGCACTTGGCTCGGCACCAATAGTAACGAGCTGAAAGGCGACGAGGTTCAAAAGTCGGTGGAATGGGCGATCGACGCTGGCTACAGGCATATCGACACTGCGGCAATCTACTCCACCGAGGACCAGGTCGGACGTGCCATTAAGAAGAAGATCGCAGAGGGAGTGGTCAAGAGAGAAGATCTGTTCGTCACTACAAAGTTATGGAACACGAACCACAAGAAGGAGGCGGTTTTGCCGGCCCTCAGGAAATCATTGAAGAATTTGGATTTGGATTACGTGGATCTATATTTAATTCACTGGCCGATCGCTCTGCACGATAACGCCTCAATTTCCCAAACAGATTACGTGGAGACGTGGCAAGCTATGACCGATGTCTTGAACCAGGGTCTGACCAAGTCCATTGGGGTGTCCAACTTCAACATTCAGCAGCTGGAGAGGCTCAAGTCTGAGGGCGGCGTCACGCCCTCCGTCCTGCAGGTCGAGATCAATTTGAACCTTCAACAGCCGGCTCTACTGGACTACTGCAAGGCTCAAGGGATAGTTGTGGTGGCGTACACCCCCTTCGGCAATCTGTTCAATCGCCAGCCGTCGGCTCCAGCGCCGCGGGCCGACGACCAGCGTCTCGTGGCCATCGCCCAAAAATACAAGAAAACCGTTCCGCAAATCGTACTCAGATATTTGGTGGAGTTAGGAGTAATTCCGATCCCGAAGTCCCTCCACAAGGACAGAGTTGAAGAGAACATCCAAATCTACGACTTCTCCCTCACTCCTGACGAGATTCAATTGCTGAAGAGTTTCGACAATGGCTACAGGACTTTATCGCCTTTGTTCTGGGCTCATTCTCCTTATTTCCCGTTTGAGAGCAAAAACTAA

Protein

MVVYKYSFVFVFAAVIHLSPSAGLAPVLKLNNGKEIPGFGIGTWLGTNSNELKGDEVQKSVEWAIDAGYRHIDTAAIYSTEDQVGRAIKKKIAEGVVKREDLFVTTKLWNTNHKKEAVLPALRKSLKNLDLDYVDLYLIHWPIALHDNASISQTDYVETWQAMTDVLNQGLTKSIGVSNFNIQQLERLKSEGGVTPSVLQVEINLNLQQPALLDYCKAQGIVVVAYTPFGNLFNRQPSAPAPRADDQRLVAIAQKYKKTVPQIVLRYLVELGVIPIPKSLHKDRVEENIQIYDFSLTPDEIQLLKSFDNGYRTLSPLFWAHSPYFPFESKN

Description

NADP-dependent oxidoreductase with high 3-dehydroecdysone reductase activity. May play a role in the regulation of molting. Has lower activity with phenylglyoxal and isatin (in vitro). Has no activity with NADH as cosubstrate. Has no activity with nitrobenzaldehyde and 3-hydroxybenzaldehyde.

Biophysicochemical Properties

0.0044 mM for 3-dehydroecdysone

Similarity

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Keywords

3D-structure   Complete proteome   Lipid metabolism   NADP   Oxidoreductase   Reference proteome   Steroid metabolism  

Feature

chain  Aldo-keto reductase AKR2E4

Uniprot

H9IVT6   I4DKQ3   A0A194RJ39   A0A1L8D6L9   A0A212FE35   A0A291P0Y7   + More
A0A2W1BLQ6   D2SNM4   A0A212FE89   A0A194PSF7   A0A2H1WKT0   A0A291P0S0   U5KCB8   G9F9F8   S4P5J6   A0A2H1WKV4   H9IVS0   A0A212EU63   A0A2A4JAY8   A0A1B2G267   A0A212FML6   I4DK12   A0A2A4K2R0   A0A1L8D6N6   A0A2A4IW06   G9F9G0   A0A194PTS4   A0A291P0W7   G9F9F7   A0A194QCQ5   A0A291P0Z6   A0A2A4JMB2   A0A194QT42   H9IVR7   D2SNV7   A0A1L8D6N3   A0A291P0V1   A0A194RIT4   A0A291P0T7   H9JTG9   A0A194PV62   I4DKC0   A0A194PUL9   H9JJU8   B8ZX07   A0A2A4JDC7   B2DBM2   G9F9G1   A0A291P0V4   A0A291P0V2   A0A212ENH0   H9JJU9   D6WBX9   A0A1Q3FN28   Q9Y020   A0A0L7QVS4   A0A2W1BC51   A0A1Z2RRK0   A0A0H5AQ19   A0A2H1VAA3   A0A212F4I6   A0A1L8D6N4   A0A194QZZ2   A0A212F244   I4DP16   A0A310S4F9   B0W806   A0A194Q090   A0A194R6Z8   A0A0C9R0F7   A0A291P0X3   A0A2A4JM15   A0A154PH71   A0A0K8TRW6   A0A088AGI5   A0A2A3EI70   V9IEZ4   A0A232EMX7   A0A1Q3F5B2   A0A023EQS8   B0WJD4   Q17DN2   K7IXG3   A0A2M3ZBK2   A0A212ERG2   A0A291P0U1   A0A1D2MB39   D9HQ59   A0A182HW15   A0A182GCB9   B0W808   A0A1B6INN6   A0A2M3ZB18   A0A2M3ZB15   A0A1Q3FMM1   A0A182PHB3   A0A182QBR1   A0A182WDU2   Q5TX68   A0A084WLY6  

EC Number

1.1.1.-

Pubmed

19121390    22651552    26354079    22118469    28986331    28756777    + More
20074338    24053512    23622113    24012638    18712529    27103441    18362917    19820115    10187849    28605547    26369729    28648823    24945155    17510324    20075255    27289101    20375075    26483478    12364791    14747013    17210077    24438588   

EMBL

BABH01038068    BABH01038069    BABH01038070    AK401871    BAM18493.1    KQ460152    + More
KPJ17355.1    GEYN01000020    JAV02109.1    AGBW02008974    OWR52019.1    MF687609    ATJ44535.1    KZ150094    PZC73640.1    EZ407153    ACX53715.1    OWR52018.1    KQ459593    KPI96371.1    ODYU01009309    SOQ53638.1    MF687575    ATJ44501.1    KC007356    AGQ45621.1    JN033703    AEW46856.1    GAIX01005504    JAA87056.1    SOQ53637.1    BABH01038066    LC375687    BBD35022.1    AGBW02012481    OWR44997.1    NWSH01002278    PCG68704.1    KU233524    ANZ02995.1    AGBW02007651    OWR54929.1    AK401630    BAM18252.1    NWSH01000243    PCG78063.1    GEYN01000021    JAV02108.1    NWSH01006101    PCG63656.1    JN033705    AEW46858.1    KPI96373.1    MF687615    ATJ44541.1    JN033702    AEW46855.1    KQ459232    KPJ02765.1    MF687618    ATJ44544.1    NWSH01001060    PCG72858.1    KQ461155    KPJ08170.1    BABH01038087    BABH01038088    BABH01038089    BABH01038090    EZ407241    ACX53798.1    GEYN01000016    JAV02113.1    MF687580    ATJ44506.1    KPJ17354.1    MF687576    ATJ44502.1    AB701383    KPI96644.1    AK401738    BAM18360.1    KPI96444.1    BABH01017849    FM955425    CAW30924.1    NWSH01001964    PCG69554.1    AB264706    BAG30781.1    JN033706    AEW46859.1    MF687578    ATJ44504.1    MF687611    ATJ44537.1    AGBW02013667    OWR43007.1    BABH01017850    BABH01017851    BABH01017852    KQ971311    EEZ99009.1    GFDL01006101    JAV28944.1    AJ131966    CAB41997.1    KQ414726    KOC62649.1    KZ150230    PZC72001.1    KY938815    ASA46456.1    LC012974    LC375688    BAR91212.1    BBD35023.1    ODYU01001496    SOQ37780.1    AGBW02010353    OWR48651.1    GEYN01000017    JAV02112.1    KQ460890    KPJ10854.1    AGBW02010783    OWR47810.1    AK403246    BAM19656.1    KQ777390    OAD52082.1    DS231856    EDS38468.1    KQ459582    KPI98997.1    KQ460878    KPJ11641.1    GBYB01001480    JAG71247.1    MF687617    ATJ44543.1    PCG72859.1    KQ434902    KZC11162.1    GDAI01000938    JAI16665.1    KZ288235    PBC31398.1    JR040676    AEY59237.1    NNAY01003257    OXU19723.1    GFDL01012352    JAV22693.1    GAPW01002622    JAC10976.1    DS231959    EDS29108.1    CH477292    EAT44543.1    AAZX01000044    GGFM01005176    MBW25927.1    AGBW02013028    OWR44065.1    MF687570    ATJ44496.1    LJIJ01002106    ODM90218.1    HM023814    ADJ58547.1    APCN01001457    JXUM01009059    KQ560275    KXJ83374.1    EDS38470.1    GECU01019162    JAS88544.1    GGFM01004986    MBW25737.1    GGFM01004985    MBW25736.1    GFDL01006287    JAV28758.1    AXCN02001099    AAAB01008804    EAA03854.4    ATLV01024323    KE525351    KFB51230.1   

Proteomes

UP000005204    UP000053240    UP000007151    UP000053268    UP000218220    UP000007266    + More
UP000053825    UP000002320    UP000076502    UP000005203    UP000242457    UP000215335    UP000008820    UP000002358    UP000094527    UP000075840    UP000069940    UP000249989    UP000075885    UP000075886    UP000075920    UP000007062    UP000030765   

PRIDE

H9IVT6    H9IVS0    H9IVR7    H9JTG9    H9JJU8    H9JJU9    + More
B0W806   

Pfam

PF00248   Aldo_ket_red        + More
PF13516   LRR_6
PF12937   F-box-like

Interpro

IPR018170   Aldo/ket_reductase_CS        + More
IPR020471   Aldo/keto_reductase       
IPR036812   NADP_OxRdtase_dom_sf       
IPR023210   NADP_OxRdtase_dom       
IPR011004   Trimer_LpxA-like_sf       
IPR001611   Leu-rich_rpt       
IPR001810   F-box_dom       
IPR032675   LRR_dom_sf       
IPR006553   Leu-rich_rpt_Cys-con_subtyp       
IPR036691   Endo/exonu/phosph_ase_sf       

SUPFAM

SSF51430   SSF51430        + More
SSF51161   SSF51161       

Gene 3D

3.20.20.100     3.80.10.10     3.60.10.10    

CDD

cd06660  Aldo_ket_red

ProteinModelPortal

H9IVT6   I4DKQ3   A0A194RJ39   A0A1L8D6L9   A0A212FE35   A0A291P0Y7   + More
A0A2W1BLQ6   D2SNM4   A0A212FE89   A0A194PSF7   A0A2H1WKT0   A0A291P0S0   U5KCB8   G9F9F8   S4P5J6   A0A2H1WKV4   H9IVS0   A0A212EU63   A0A2A4JAY8   A0A1B2G267   A0A212FML6   I4DK12   A0A2A4K2R0   A0A1L8D6N6   A0A2A4IW06   G9F9G0   A0A194PTS4   A0A291P0W7   G9F9F7   A0A194QCQ5   A0A291P0Z6   A0A2A4JMB2   A0A194QT42   H9IVR7   D2SNV7   A0A1L8D6N3   A0A291P0V1   A0A194RIT4   A0A291P0T7   H9JTG9   A0A194PV62   I4DKC0   A0A194PUL9   H9JJU8   B8ZX07   A0A2A4JDC7   B2DBM2   G9F9G1   A0A291P0V4   A0A291P0V2   A0A212ENH0   H9JJU9   D6WBX9   A0A1Q3FN28   Q9Y020   A0A0L7QVS4   A0A2W1BC51   A0A1Z2RRK0   A0A0H5AQ19   A0A2H1VAA3   A0A212F4I6   A0A1L8D6N4   A0A194QZZ2   A0A212F244   I4DP16   A0A310S4F9   B0W806   A0A194Q090   A0A194R6Z8   A0A0C9R0F7   A0A291P0X3   A0A2A4JM15   A0A154PH71   A0A0K8TRW6   A0A088AGI5   A0A2A3EI70   V9IEZ4   A0A232EMX7   A0A1Q3F5B2   A0A023EQS8   B0WJD4   Q17DN2   K7IXG3   A0A2M3ZBK2   A0A212ERG2   A0A291P0U1   A0A1D2MB39   D9HQ59   A0A182HW15   A0A182GCB9   B0W808   A0A1B6INN6   A0A2M3ZB18   A0A2M3ZB15   A0A1Q3FMM1   A0A182PHB3   A0A182QBR1   A0A182WDU2   Q5TX68   A0A084WLY6  

PDB

3WCZ     E-value=2.63752e-83,     Score=785

KEGG

101746802    K00011 aldehyde reductase [EC:1.1.1.21] | (RefSeq) aldo-keto reductase AKR2E4-like

PATHWAY

00040    Pentose and glucuronate interconversions - Bombyx mori (domestic silkworm)
00051    Fructose and mannose metabolism - Bombyx mori (domestic silkworm)
00052    Galactose metabolism - Bombyx mori (domestic silkworm)
00561    Glycerolipid metabolism - Bombyx mori (domestic silkworm)
00790    Folate biosynthesis - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)

GO

GO:0016491   GO:0008202   GO:0000166   GO:0005829   GO:0008106   GO:0004032   GO:0005622   GO:0005840   GO:0006412   GO:0007275   GO:0004812   GO:0006418   GO:0004887   GO:0003707   GO:0016021   GO:0055114  

PANTHER

PTHR11732  

SignalP

Position:   1 - 23,         Likelihood:  0.949342

 

 

Length:

331

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

15.37471

Exp number, first 60 AAs:

15.36356

Total prob of N-in:

0.72408

POSSIBLE N-term signal

sequence

outside

1  -  331

 

 

Pi

279.516623

Theta

169.167658

Tajima's D

2.945845

CLR

0.125962

CSRT

0.975351232438378

Interpretation

Uncertain