Gene

KWMTBOMO00984  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA001351

Annotation

PREDICTED:_aldo-keto_reductase_AKR2E4-like_[Amyelois_transitella]

Location

Bomo_Chr2(-):7882359-7891307      View in EpiBrowser

Full name

Aldo-keto reductase AKR2E4      

Alternative Name

3-dehydroecdysone reductase
Aldo-keto reductase 2E

Location in the cell

Cytoplasmic   Reliability : 1.502 PlasmaMembrane   Reliability : 1.681

CDS

ATGGTCATTCCATTCGCAATGGGCAAGTGGGCCGGTTTCTTGTTTTGCCTGAGCGCGCTGTGTTTTACTAGCGCTGAACTGATCGTGCCGACATTACAACTGAGTAGTGGTGGGAACATACCAGTGGTAGCACTCGGAACATGGCTGGGACATCGACCTAAAGGAAAAGTAGTTCAAGTAACCAATGACACAGAGCAAGCTGTCATATACGCGTTGGAAGCTGGATACACACACATAGACACCGCGTACAAATATGGAATCGAGGACCAAGTGGGAAGGGCCCTGACCAAGAAATTCTCAGAAGGACTAAAAAGAGAATCTATCTTTATAACTACCAAGCTCTGGAATTCCTACCACGAGAGAGAGCAAGTGATCCCAGCGCTGAGGAAGTCCCTTGAAAATCTCAACCTGGAATACGTCGATCTGTATTTGATTCACTGGCCCATAGCTATGTTTGAGAACGATACCTTACTCGACAACGTGGACTTTCTGGACACGTGGCGGGGTATGATCGACGCCAAGAAGCTCGGCCTGACCAAATCCATAGGCATCTCCAACTTTAACCAAGAACAAATCCAGAGGATCATCGACAGCGGACTGGAGAAACCTTCGGCTTTGCAGATCGAGTTAAACCTCAACCTGCAACAACCAGAGCTCTTCAAGTTCTGCCGCGAAAACGACATAGTGGTGATGGCGTACACTCCCTTCGGGTCGCTGTTCCCGAGCAAGGCTCAACCAGACGCTCCTCCGCCGAGGATTGACGATGAACGCCTCGTCAGTATCGCCCAGAAATACAACAAAACTGTCCCGCAAGTTGCTCTTCGCTTTCTGATCGAGTTAGGAGCCGTCGTTCTACCCAAGTCGGTGCATAAGGAGAGGATCGAGCAGAACATACAGCTGTTCGACTTCAGTCTGACTCCTGAGGAGTTCAACCTGCTGAAAAGCTTTGACACCGGCTACAGGACCTTGCCACAGACCAAATGGAGCAAACATCCTGACTACCCGTTCCAGTTAGCATGA

Protein

MVIPFAMGKWAGFLFCLSALCFTSAELIVPTLQLSSGGNIPVVALGTWLGHRPKGKVVQVTNDTEQAVIYALEAGYTHIDTAYKYGIEDQVGRALTKKFSEGLKRESIFITTKLWNSYHEREQVIPALRKSLENLNLEYVDLYLIHWPIAMFENDTLLDNVDFLDTWRGMIDAKKLGLTKSIGISNFNQEQIQRIIDSGLEKPSALQIELNLNLQQPELFKFCRENDIVVMAYTPFGSLFPSKAQPDAPPPRIDDERLVSIAQKYNKTVPQVALRFLIELGAVVLPKSVHKERIEQNIQLFDFSLTPEEFNLLKSFDTGYRTLPQTKWSKHPDYPFQLA

Description

NADP-dependent oxidoreductase with high 3-dehydroecdysone reductase activity. May play a role in the regulation of molting. Has lower activity with phenylglyoxal and isatin (in vitro). Has no activity with NADH as cosubstrate. Has no activity with nitrobenzaldehyde and 3-hydroxybenzaldehyde.

Biophysicochemical Properties

0.0044 mM for 3-dehydroecdysone

Similarity

Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Belongs to the class I-like SAM-binding methyltransferase superfamily. Trm1 family.

Keywords

3D-structure   Complete proteome   Lipid metabolism   NADP   Oxidoreductase   Reference proteome   Steroid metabolism  

Feature

chain  Aldo-keto reductase AKR2E4

Uniprot

H9IVS0   U5KCB8   A0A194PSF7   G9F9F8   A0A194RJ39   S4P5J6   + More
A0A2H1WKV4   A0A212EU63   A0A1L8D6L9   A0A2A4JAY8   H9IVT6   I4DKQ3   A0A212FE89   A0A212FE35   A0A291P0Y7   D2SNM4   A0A2W1BLQ6   A0A2H1WKT0   D9HQ59   A0A291P0S0   G9D4T1   G9D4S7   G9D4S4   G9D4T6   G9D4T4   G9D4T3   G9D4T5   G9D4S6   G9D4T2   A0A194QT42   G9F9F7   G9D4S9   A0A194QCQ5   A0A194PTS4   A0A212F4I6   A0A1L8D6N6   G9D4S5   G9F9G0   A0A194RIT4   A0A212FML6   G9D4T0   A0A291P0W7   A0A291P0V1   A0A2A4JDC7   A0A291P0Z6   A0A2A4JMB2   A0A0L7QVS4   A0A088AGI5   A0A2A3EI70   V9IEZ4   A0A2H1VAA3   A0A291P0T7   H9JTG9   D2SNV7   A0A2A4K2R0   I4DK12   A0A2A4IW06   A0A1B2G267   A0A154PH71   A0A310S4F9   A0A1B6INN6   A0A194PV62   F4WLB3   B2DBM2   I4DP16   D6WBX9   A0A194Q090   A0A194R6Z8   A0A0K8TRW6   A0A1Z2RRK0   G9F9G1   A0A1L8D6N3   A0A195FXI0   A0A2W1BC51   A0A026WTG4   A0A291P0X3   A0A1W4W9Z3   A0A291P0V4   A0A0T6AZ90   A0A182GCC4   A0A023EPH2   A0A2H1VGR3   A0A194PUL9   A0A182KKP6   H9JJU8   A0A182HW15   H9IVR7   Q5TX68   A0A023EQM7   A0A182V431   A0A182TDQ5   A0A2H1VAB6   D2A296   A0A084WLY6   A0A291P0R3   Q9Y020   A0A182WUG3   A0A291P0V2   A0A1Q3FN84  

EC Number

1.1.1.-

Pubmed

19121390    24053512    26354079    23622113    22118469    22651552    + More
28986331    20074338    28756777    20375075    21967427    24012638    21719571    18712529    18362917    19820115    26369729    28605547    24508170    26483478    24945155    20966253    12364791    14747013    17210077    24438588    10187849   

EMBL

BABH01038066    LC375687    BBD35022.1    KC007356    AGQ45621.1    KQ459593    + More
KPI96371.1    JN033703    AEW46856.1    KQ460152    KPJ17355.1    GAIX01005504    JAA87056.1    ODYU01009309    SOQ53637.1    AGBW02012481    OWR44997.1    GEYN01000020    JAV02109.1    NWSH01002278    PCG68704.1    BABH01038068    BABH01038069    BABH01038070    AK401871    BAM18493.1    AGBW02008974    OWR52018.1    OWR52019.1    MF687609    ATJ44535.1    EZ407153    ACX53715.1    KZ150094    PZC73640.1    SOQ53638.1    HM023814    ADJ58547.1    MF687575    ATJ44501.1    JF777334    AEU11679.1    JF777330    AEU11675.1    JF777327    AEU11672.1    JF777339    AEU11684.1    JF777337    AEU11682.1    JF777336    AEU11681.1    JF777338    AEU11683.1    JF777329    AEU11674.1    JF777335    AEU11680.1    KQ461155    KPJ08170.1    JN033702    AEW46855.1    JF777332    AEU11677.1    KQ459232    KPJ02765.1    KPI96373.1    AGBW02010353    OWR48651.1    GEYN01000021    JAV02108.1    JF777328    AEU11673.1    JN033705    AEW46858.1    KPJ17354.1    AGBW02007651    OWR54929.1    JF777333    AEU11678.1    MF687615    ATJ44541.1    MF687580    ATJ44506.1    NWSH01001964    PCG69554.1    MF687618    ATJ44544.1    NWSH01001060    PCG72858.1    KQ414726    KOC62649.1    KZ288235    PBC31398.1    JR040676    AEY59237.1    ODYU01001496    SOQ37780.1    MF687576    ATJ44502.1    AB701383    EZ407241    ACX53798.1    NWSH01000243    PCG78063.1    AK401630    BAM18252.1    NWSH01006101    PCG63656.1    KU233524    ANZ02995.1    KQ434902    KZC11162.1    KQ777390    OAD52082.1    GECU01019162    JAS88544.1    KPI96644.1    GL888207    EGI65076.1    AB264706    BAG30781.1    AK403246    BAM19656.1    KQ971311    EEZ99009.1    KQ459582    KPI98997.1    KQ460878    KPJ11641.1    GDAI01000938    JAI16665.1    KY938815    ASA46456.1    JN033706    AEW46859.1    GEYN01000016    JAV02113.1    KQ981169    KYN45375.1    KZ150230    PZC72001.1    KK107109    EZA59355.1    MF687617    ATJ44543.1    MF687578    ATJ44504.1    LJIG01022464    KRT80438.1    JXUM01009068    KQ560275    KXJ83379.1    GAPW01002693    JAC10905.1    ODYU01002492    SOQ40039.1    KPI96444.1    BABH01017849    APCN01001457    BABH01038087    BABH01038088    BABH01038089    BABH01038090    AAAB01008804    EAA03854.4    GAPW01002694    JAC10904.1    SOQ37779.1    KQ971338    EFA02158.1    ATLV01024323    KE525351    KFB51230.1    MF687571    ATJ44497.1    AJ131966    CAB41997.1    MF687611    ATJ44537.1    GFDL01006014    JAV29031.1   

Proteomes

UP000005204    UP000053268    UP000053240    UP000007151    UP000218220    UP000053825    + More
UP000005203    UP000242457    UP000076502    UP000007755    UP000007266    UP000078541    UP000053097    UP000192223    UP000069940    UP000249989    UP000075882    UP000075840    UP000007062    UP000075903    UP000075902    UP000030765    UP000076407   

PRIDE

H9IVS0    H9IVT6    H9JTG9    H9JJU8    H9IVR7   

Pfam

PF00248   Aldo_ket_red        + More
PF02005   TRM
PF13516   LRR_6
PF12937   F-box-like

Interpro

IPR023210   NADP_OxRdtase_dom        + More
IPR036812   NADP_OxRdtase_dom_sf       
IPR018170   Aldo/ket_reductase_CS       
IPR020471   Aldo/keto_reductase       
IPR011004   Trimer_LpxA-like_sf       
IPR036691   Endo/exonu/phosph_ase_sf       
IPR029063   SAM-dependent_MTases       
IPR042296   tRNA_met_Trm1_C       
IPR002905   Trm1       
IPR001611   Leu-rich_rpt       
IPR001810   F-box_dom       
IPR032675   LRR_dom_sf       
IPR006553   Leu-rich_rpt_Cys-con_subtyp       

SUPFAM

SSF51430   SSF51430        + More
SSF51161   SSF51161       
SSF53335   SSF53335       

Gene 3D

3.20.20.100     3.60.10.10     3.30.56.70     3.80.10.10    

CDD

cd06660  Aldo_ket_red

ProteinModelPortal

H9IVS0   U5KCB8   A0A194PSF7   G9F9F8   A0A194RJ39   S4P5J6   + More
A0A2H1WKV4   A0A212EU63   A0A1L8D6L9   A0A2A4JAY8   H9IVT6   I4DKQ3   A0A212FE89   A0A212FE35   A0A291P0Y7   D2SNM4   A0A2W1BLQ6   A0A2H1WKT0   D9HQ59   A0A291P0S0   G9D4T1   G9D4S7   G9D4S4   G9D4T6   G9D4T4   G9D4T3   G9D4T5   G9D4S6   G9D4T2   A0A194QT42   G9F9F7   G9D4S9   A0A194QCQ5   A0A194PTS4   A0A212F4I6   A0A1L8D6N6   G9D4S5   G9F9G0   A0A194RIT4   A0A212FML6   G9D4T0   A0A291P0W7   A0A291P0V1   A0A2A4JDC7   A0A291P0Z6   A0A2A4JMB2   A0A0L7QVS4   A0A088AGI5   A0A2A3EI70   V9IEZ4   A0A2H1VAA3   A0A291P0T7   H9JTG9   D2SNV7   A0A2A4K2R0   I4DK12   A0A2A4IW06   A0A1B2G267   A0A154PH71   A0A310S4F9   A0A1B6INN6   A0A194PV62   F4WLB3   B2DBM2   I4DP16   D6WBX9   A0A194Q090   A0A194R6Z8   A0A0K8TRW6   A0A1Z2RRK0   G9F9G1   A0A1L8D6N3   A0A195FXI0   A0A2W1BC51   A0A026WTG4   A0A291P0X3   A0A1W4W9Z3   A0A291P0V4   A0A0T6AZ90   A0A182GCC4   A0A023EPH2   A0A2H1VGR3   A0A194PUL9   A0A182KKP6   H9JJU8   A0A182HW15   H9IVR7   Q5TX68   A0A023EQM7   A0A182V431   A0A182TDQ5   A0A2H1VAB6   D2A296   A0A084WLY6   A0A291P0R3   Q9Y020   A0A182WUG3   A0A291P0V2   A0A1Q3FN84  

PDB

3WCZ     E-value=4.78474e-79,     Score=749

KEGG

101746566    K00011 aldehyde reductase [EC:1.1.1.21] | (RefSeq) aldo-keto reductase AKR2E4-like

PATHWAY

00040    Pentose and glucuronate interconversions - Bombyx mori (domestic silkworm)
00051    Fructose and mannose metabolism - Bombyx mori (domestic silkworm)
00052    Galactose metabolism - Bombyx mori (domestic silkworm)
00561    Glycerolipid metabolism - Bombyx mori (domestic silkworm)
00790    Folate biosynthesis - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)

GO

GO:0016491   GO:0008202   GO:0005829   GO:0008106   GO:0004032   GO:0004809   GO:0000049   GO:0005622   GO:0005840   GO:0006412   GO:0007275   GO:0004812   GO:0006418   GO:0004887   GO:0003707   GO:0016021   GO:0055114  

PANTHER

PTHR11732   PTHR10631  

SignalP

Position:   1 - 25,         Likelihood:  0.886306

 

 

Length:

339

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

10.96548

Exp number, first 60 AAs:

10.95798

Total prob of N-in:

0.47548

POSSIBLE N-term signal

sequence

outside

1  -  339

 

 

Pi

273.115874

Theta

171.795697

Tajima's D

1.299823

CLR

0.073705

CSRT

0.733963301834908

Interpretation

Uncertain