Gene

KWMTBOMO00940  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA009801

Annotation

seminal_fluid_protein_CSSFP006_[Chilo_suppressalis]

Location

Bomo_Chr2(+):6871373-6879413      View in EpiBrowser

Full name

Aldo-keto reductase AKR2E4      

Alternative Name

3-dehydroecdysone reductase
Aldo-keto reductase 2E

Location in the cell

Cytoplasmic   Reliability : 2.372 Nuclear   Reliability : 1.767

CDS

ATGGACAATCAGAGAGACGTGGTGAAACAATTGGAGAAGTCTTTGGCGAGATTGAACATGAGCTACGTCGATCTGTACTTGATACACAATCCAGTTGCTTTTAATCCTGGATTCAAAGGTTTCGACATTGTAGATTACTTGGACACCTGGAAAGGCATGGAGGAAGCTAAAAATCTAGGCCTAGCTAAATCTATAGGCATATCTAACTTCAACACCACACAAATAGACAGAATATTAGAGAATGGCCAAATAAAACCTTCAGTTTTACAAGTGGAAGTGAATCTACATCTTGGTCAAGATAAACTTATTGACTATTGCAAACGTAACGGTATAGTTGTTATGGCGTTTTCCCCGTTCGGCCCGATGTTCCACGACAGCTTACCCTCAATACGCCCCGACGACCCCACGCTCGTGGCCATTGCCGAAAAACACGGAAAAAGCGTTACACAAATCGTTCTTCGGTATTTGATCCAAAAGGGGGTAGTTCCAATCCCTAAATCAGTTAAGAAGAACCGCATTGAGGAAAACATTGACGTTTTCGATTTCAATTTGTCATCTGAGGATATGAATAAGATTGGTACATTCAACAAGGAGAAACGCACAGTCGGTATGTCTTTCTGGCAGGATCATCCTTACTATCCGTTCGAGAAGAGTGACCAAGTTCATGACAATCCTTTCGAATCGGCAAATTGA

Protein

MDNQRDVVKQLEKSLARLNMSYVDLYLIHNPVAFNPGFKGFDIVDYLDTWKGMEEAKNLGLAKSIGISNFNTTQIDRILENGQIKPSVLQVEVNLHLGQDKLIDYCKRNGIVVMAFSPFGPMFHDSLPSIRPDDPTLVAIAEKHGKSVTQIVLRYLIQKGVVPIPKSVKKNRIEENIDVFDFNLSSEDMNKIGTFNKEKRTVGMSFWQDHPYYPFEKSDQVHDNPFESAN

Description

NADP-dependent oxidoreductase with high 3-dehydroecdysone reductase activity. May play a role in the regulation of molting. Has lower activity with phenylglyoxal and isatin (in vitro). Has no activity with NADH as cosubstrate. Has no activity with nitrobenzaldehyde and 3-hydroxybenzaldehyde.

Biophysicochemical Properties

0.0044 mM for 3-dehydroecdysone

Similarity

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Keywords

3D-structure   Complete proteome   Lipid metabolism   NADP   Oxidoreductase   Reference proteome   Steroid metabolism  

Feature

chain  Aldo-keto reductase AKR2E4

Uniprot

H9JJV0   D2SNV7   A0A291P0W7   G9F9F7   I4DK12   A0A2H1UZN2   + More
A0A194PV62   A0A1L8D6N4   A0A291P0R3   B8ZX08   A0A2A4JPZ0   U5KCB8   A0A194PSF7   G9F9G0   S4P5J6   A0A212FE89   I4DP16   A0A1L8D6L9   G9F9F8   A0A194RJ39   A0A076FT04   A0A2W1BLQ6   A0A2H1WKV4   A0A212FE35   A0A291P0T7   A0A291P0Y7   A0A194Q090   A0A2H1WKT0   A0A2A4JMB2   A0A1L8D6N6   A0A194R6Z8   H9IVT6   H9JTG9   A0A2A4K2R0   A0A2A4IW06   H9IVS0   A0A212EU63   A0A1B2G267   A0A212FML6   A0A2H1VAA3   A0A1L8D6N3   A0A0N1IPK2   A0A2W1BC51   B2DBM2   A0A1Z2RRK0   D2SNM4   A0A2A4JDC7   A0A194QT42   A0A194QCQ5   H9IVR7   I4DKQ3   A0A2H1WLD2   D6WBX9   A0A291P0S0   A0A194PUL9   A0A291P0V1   A0A291P0Z6   G9F9F9   A0A0T6B8Q2   A0A212F4I6   A0A2A4J392   A0A291P0X3   G9F9G1   A0A2A4J1K6   A0A2A4JAY8   A0A0N1PGT3   A0A194RIT4   A0A291P0V4   I4DKC0   A0A2H1WDA9   U5KCB5   A0A2A4JM15   A0A212ENH0   A0A2J7QL56   A0A2P8XDR5   A0A212ERG2   A0A291P0V2   B8ZX07   A0A194QZZ2   A0A2W1BEQ9   A0A291P0W3   A0A1W4WL13   Q9Y020   A0A2H1WN92   A0A194PTS4   A0A212F244   A0A291P0U1   A0A2W1BGS7   A0A2W1BI87   D2A296   A0A2J7Q6Z4   J3JYE0   A0A0L7K3F6   H9JJU8   A0A2H1W804   A0A291P0U2   A0A291P0Y9   A0A2P2I1M8   V5GT06   U5KBY4  

EC Number

1.1.1.-

Pubmed

19121390    20074338    28986331    22651552    26354079    24053512    + More
23622113    22118469    24817326    28756777    24012638    18712529    28605547    18362917    19820115    29403074    10187849    22516182    26227816   

EMBL

BABH01017856    EZ407241    ACX53798.1    MF687615    ATJ44541.1    JN033702    + More
AEW46855.1    AK401630    BAM18252.1    ODYU01000004    SOQ34051.1    KQ459593    KPI96644.1    GEYN01000017    JAV02112.1    MF687571    ATJ44497.1    FM955425    CAW30925.1    NWSH01000872    PCG73778.1    KC007356    AGQ45621.1    KPI96371.1    JN033705    AEW46858.1    GAIX01005504    JAA87056.1    AGBW02008974    OWR52018.1    AK403246    BAM19656.1    GEYN01000020    JAV02109.1    JN033703    AEW46856.1    KQ460152    KPJ17355.1    KF960772    AII21974.1    KZ150094    PZC73640.1    ODYU01009309    SOQ53637.1    OWR52019.1    MF687576    ATJ44502.1    MF687609    ATJ44535.1    KQ459582    KPI98997.1    SOQ53638.1    NWSH01001060    PCG72858.1    GEYN01000021    JAV02108.1    KQ460878    KPJ11641.1    BABH01038068    BABH01038069    BABH01038070    AB701383    NWSH01000243    PCG78063.1    NWSH01006101    PCG63656.1    BABH01038066    LC375687    BBD35022.1    AGBW02012481    OWR44997.1    KU233524    ANZ02995.1    AGBW02007651    OWR54929.1    ODYU01001496    SOQ37780.1    GEYN01000016    JAV02113.1    KQ460280    KPJ16304.1    KZ150230    PZC72001.1    AB264706    BAG30781.1    KY938815    ASA46456.1    EZ407153    ACX53715.1    NWSH01001964    PCG69554.1    KQ461155    KPJ08170.1    KQ459232    KPJ02765.1    BABH01038087    BABH01038088    BABH01038089    BABH01038090    AK401871    BAM18493.1    ODYU01009410    SOQ53817.1    KQ971311    EEZ99009.1    MF687575    ATJ44501.1    KPI96444.1    MF687580    ATJ44506.1    MF687618    ATJ44544.1    JN033704    AEW46857.1    LJIG01009120    KRT83716.1    AGBW02010353    OWR48651.1    NWSH01003732    PCG65872.1    MF687617    ATJ44543.1    JN033706    AEW46859.1    PCG65871.1    NWSH01002278    PCG68704.1    KPJ16305.1    KPJ17354.1    MF687578    ATJ44504.1    AK401738    BAM18360.1    ODYU01007882    SOQ51041.1    KC007346    AGQ45611.1    PCG72859.1    AGBW02013667    OWR43007.1    NEVH01013254    PNF29307.1    PYGN01002639    PSN30150.1    AGBW02013028    OWR44065.1    MF687611    ATJ44537.1    CAW30924.1    KQ460890    KPJ10854.1    PZC73639.1    MF687607    ATJ44533.1    AJ131966    CAB41997.1    ODYU01009843    SOQ54535.1    KPI96373.1    AGBW02010783    OWR47810.1    MF687570    ATJ44496.1    PZC72000.1    KZ150106    PZC73415.1    KQ971338    EFA02158.1    NEVH01017450    PNF24350.1    BT128266    AEE63226.1    JTDY01011970    KOB52355.1    BABH01017849    ODYU01006799    SOQ48982.1    MF687572    ATJ44498.1    MF687608    ATJ44534.1    IACF01002285    LAB67945.1    GALX01003699    JAB64767.1    KC007350    AGQ45615.1   

Proteomes

UP000005204    UP000053268    UP000218220    UP000007151    UP000053240    UP000007266    + More
UP000235965    UP000245037    UP000192223    UP000037510   

PRIDE

H9JJV0    H9IVT6    H9JTG9    H9IVS0    H9IVR7    H9JJU8    + More

Pfam

PF00248   Aldo_ket_red        + More
PF13516   LRR_6
PF12937   F-box-like

Interpro

IPR023210   NADP_OxRdtase_dom        + More
IPR036812   NADP_OxRdtase_dom_sf       
IPR018170   Aldo/ket_reductase_CS       
IPR020471   Aldo/keto_reductase       
IPR011004   Trimer_LpxA-like_sf       
IPR036691   Endo/exonu/phosph_ase_sf       
IPR001611   Leu-rich_rpt       
IPR001810   F-box_dom       
IPR032675   LRR_dom_sf       
IPR006553   Leu-rich_rpt_Cys-con_subtyp       

SUPFAM

SSF51430   SSF51430        + More
SSF51161   SSF51161       

Gene 3D

3.20.20.100     3.60.10.10     3.80.10.10    

CDD

cd06660  Aldo_ket_red

ProteinModelPortal

H9JJV0   D2SNV7   A0A291P0W7   G9F9F7   I4DK12   A0A2H1UZN2   + More
A0A194PV62   A0A1L8D6N4   A0A291P0R3   B8ZX08   A0A2A4JPZ0   U5KCB8   A0A194PSF7   G9F9G0   S4P5J6   A0A212FE89   I4DP16   A0A1L8D6L9   G9F9F8   A0A194RJ39   A0A076FT04   A0A2W1BLQ6   A0A2H1WKV4   A0A212FE35   A0A291P0T7   A0A291P0Y7   A0A194Q090   A0A2H1WKT0   A0A2A4JMB2   A0A1L8D6N6   A0A194R6Z8   H9IVT6   H9JTG9   A0A2A4K2R0   A0A2A4IW06   H9IVS0   A0A212EU63   A0A1B2G267   A0A212FML6   A0A2H1VAA3   A0A1L8D6N3   A0A0N1IPK2   A0A2W1BC51   B2DBM2   A0A1Z2RRK0   D2SNM4   A0A2A4JDC7   A0A194QT42   A0A194QCQ5   H9IVR7   I4DKQ3   A0A2H1WLD2   D6WBX9   A0A291P0S0   A0A194PUL9   A0A291P0V1   A0A291P0Z6   G9F9F9   A0A0T6B8Q2   A0A212F4I6   A0A2A4J392   A0A291P0X3   G9F9G1   A0A2A4J1K6   A0A2A4JAY8   A0A0N1PGT3   A0A194RIT4   A0A291P0V4   I4DKC0   A0A2H1WDA9   U5KCB5   A0A2A4JM15   A0A212ENH0   A0A2J7QL56   A0A2P8XDR5   A0A212ERG2   A0A291P0V2   B8ZX07   A0A194QZZ2   A0A2W1BEQ9   A0A291P0W3   A0A1W4WL13   Q9Y020   A0A2H1WN92   A0A194PTS4   A0A212F244   A0A291P0U1   A0A2W1BGS7   A0A2W1BI87   D2A296   A0A2J7Q6Z4   J3JYE0   A0A0L7K3F6   H9JJU8   A0A2H1W804   A0A291P0U2   A0A291P0Y9   A0A2P2I1M8   V5GT06   U5KBY4  

PDB

3WCZ     E-value=9.09725e-57,     Score=555

KEGG

101740752    no KO assigned | (RefSeq) aldo-keto reductase AKR2E4-like

GO

GO:0016491   GO:0008202   GO:0005829   GO:0008106   GO:0004032   GO:0006355   GO:0043565   GO:0005634   GO:0007275   GO:0004812   GO:0006418   GO:0004887   GO:0003707   GO:0016021   GO:0055114  

PANTHER

PTHR11732  

Length:

230

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.20579

Exp number, first 60 AAs:

0.08809

Total prob of N-in:

0.31243

outside

1  -  230

 

 

Pi

209.027149

Theta

160.292614

Tajima's D

0.98429

CLR

0.176549

CSRT

0.655417229138543

Interpretation

Uncertain