Gene

KWMTBOMO00939  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA009800

Annotation

PREDICTED:_aldo-keto_reductase_AKR2E4-like_isoform_X2_[Bombyx_mori]

Location

Bomo_Chr2(+):6848823-6859047      View in EpiBrowser

Full name

Aldo-keto reductase AKR2E4      

Alternative Name

3-dehydroecdysone reductase
Aldo-keto reductase 2E

Location in the cell

Cytoplasmic   Reliability : 3.347

CDS

ATGAATAAGGTGAATGTACCCAATTTCAAACTAAACAATGGCGATGAGATCCCAGCTCTGGGCTATGGTACGTGGTTAGGAGTCAGCGAAAAGTCGTCGGGTGCTGATCAACCTGCCGAAGAATTTGGTTTTGATTTCGACGGCACTGACATCCCGAAGCTGTTGGACGCTCTCTCCTACGCCATAGACATCGGCTATCGTCACATAGACACAGCGCACTTCTACAGGGTTGAGCCAGAGATAGGCCAAGTCGTCCAGGAGAAAATAAATGAGGGTGTCGTAAGGAGAGAAGATCTTTTTATAACAACCAAGGTTTGGCAACACTACCATCGTGCAGCCGATGTTGAAGTCTCGTTGAGGGCTTCTCTGCATCGTCTAGGACTTGACCACGTCAACTTAGTGCTTATGCACTGGCCTATGTCAATTAGTCAAGAAGGGGTAGACGAAAAGATCGACTACCTGGAGACGTGGAGAGGGTTTGAGGAGGTCCTCAAGAAGGGGCTGACGAAGGCCATTGGCGTGTCCAACTTCAACATCGAACAGATGAAGAGGTTACTAACCAACTGCAACGTGCCGCCCGCCGTCAACCAGATCGAGGTCAACTTGAACTTGTCTCAAGCAGATTTAGTCGATTACTGTCAAGCGAATGAGGTTGTGGTTGTGGCGTATTCGCCTTTCGGGACCATGGTTCCGTCTCGAGCAACCCTCAACAGTCCAGAGCCGAAGCTTGACAACCCTGCCATGCTGGCTATAGGAAGGAAATATGGGAAGACGGTCACTCAAGTCAATTTGGGATACTTGTACCAGCGCGGTATAGTGTCTATACCGAAGACTGTGACCAAGAGTCGAGTGCTGGAGAACGCCTCGATCTTCGACTTCCAGCTGGACGACGAGGACGTCGCCACTCTGGCGCAGTTCGACAACGGCTTCCGGACGGTCAGACCGCTGTTCTGGCAGCCCTACGAGAACTATCCCTTCGACGTGGTCCCTGGACAGAAGCACGTCGACATACCGATCGCCATGCGCAAATGGAAGAATGGTGCCAACGGTGACATCGATTAA

Protein

MNKVNVPNFKLNNGDEIPALGYGTWLGVSEKSSGADQPAEEFGFDFDGTDIPKLLDALSYAIDIGYRHIDTAHFYRVEPEIGQVVQEKINEGVVRREDLFITTKVWQHYHRAADVEVSLRASLHRLGLDHVNLVLMHWPMSISQEGVDEKIDYLETWRGFEEVLKKGLTKAIGVSNFNIEQMKRLLTNCNVPPAVNQIEVNLNLSQADLVDYCQANEVVVVAYSPFGTMVPSRATLNSPEPKLDNPAMLAIGRKYGKTVTQVNLGYLYQRGIVSIPKTVTKSRVLENASIFDFQLDDEDVATLAQFDNGFRTVRPLFWQPYENYPFDVVPGQKHVDIPIAMRKWKNGANGDID

Description

NADP-dependent oxidoreductase with high 3-dehydroecdysone reductase activity. May play a role in the regulation of molting. Has lower activity with phenylglyoxal and isatin (in vitro). Has no activity with NADH as cosubstrate. Has no activity with nitrobenzaldehyde and 3-hydroxybenzaldehyde.

Biophysicochemical Properties

0.0044 mM for 3-dehydroecdysone

Similarity

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Keywords

3D-structure   Complete proteome   Lipid metabolism   NADP   Oxidoreductase   Reference proteome   Steroid metabolism  

Feature

chain  Aldo-keto reductase AKR2E4

Uniprot

A0A0H5AQ19   H9JJU9   H9JJU8   A0A291P0V2   A0A291P0V4   B8ZX07   + More
G9F9G1   I4DKC0   A0A0N1PGT3   A0A212ENH0   U5KCB5   A0A2A4J392   A0A2A4J1K6   A0A212ERG2   A0A194PV62   H9IVT6   I4DKQ3   A0A0L7KNM6   A0A2A4IW06   A0A194RJ39   A0A2A4K2R0   A0A291P0Y7   A0A1L8D6L9   Q9Y020   I4DP16   A0A2W1BLQ6   A0A291P0T7   A0A1B2G267   A0A2A4JMB2   A0A194Q090   H9IVR7   U5KCB8   A0A212FE89   A0A212FE35   A0A194R6Z8   G9F9G0   A0A182N3T0   A0A1Z2RRK0   A0A1L8D6N3   A0A182QZ12   A0A2W1BC51   A0A182M9D3   V5GQ24   A0A2H1WKT0   D2SNM4   A0A291P0Y9   A0A2H1VAA3   A0A291P0U2   A0A194PUL9   A0A2A4IYA3   A0A291P0S0   U5KBY4   A0A182WDU2   A0A0C9QAV8   V5GT06   A0A182RUK0   A0A0F6Q2W6   A0A291P0W7   A0A182IZ90   A0A212F244   G9F9F4   A0A1D2MB39   A0A182YBI3   A0A182SQ44   A0A182HW15   A0A182V431   A0A2M4BT41   W5JBU5   A0A194PKB3   A0A084WLY6   D2SNV7   A0A291P0X3   A0A067QJF4   A0A0C9R605   A0A2M4BT73   A0A2M3ZB15   A0A2M3ZBK2   A0A2M3ZB18   A0A182KKP6   S4P5J6   Q5TX68   A0A182TDQ5   A0A212EU63   A0A182PHB3   A0A2M4ANN0   A0A182WUG3   A0A2M4ANT1   W5JAV1   H9JTG9   A0A2M4BT68   A0A2M4BU17   A0A2M4ANA7   B3M7Z0   H9IVS0   A0A182JS79   A0A232EMX7   F4WLB4  

EC Number

1.1.1.-

Pubmed

19121390    27103441    28986331    22651552    26354079    22118469    + More
24053512    26227816    10187849    28756777    28605547    20074338    27289101    25244985    20920257    23761445    24438588    24845553    20966253    23622113    12364791    14747013    17210077    24012638    17994087    28648823    21719571   

EMBL

LC012974    LC375688    BAR91212.1    BBD35023.1    BABH01017850    BABH01017851    + More
BABH01017852    BABH01017849    MF687611    ATJ44537.1    MF687578    ATJ44504.1    FM955425    CAW30924.1    JN033706    AEW46859.1    AK401738    BAM18360.1    KQ460280    KPJ16305.1    AGBW02013667    OWR43007.1    KC007346    AGQ45611.1    NWSH01003732    PCG65872.1    PCG65871.1    AGBW02013028    OWR44065.1    KQ459593    KPI96644.1    BABH01038068    BABH01038069    BABH01038070    AK401871    BAM18493.1    JTDY01008418    KOB64579.1    NWSH01006101    PCG63656.1    KQ460152    KPJ17355.1    NWSH01000243    PCG78063.1    MF687609    ATJ44535.1    GEYN01000020    JAV02109.1    AJ131966    CAB41997.1    AK403246    BAM19656.1    KZ150094    PZC73640.1    MF687576    ATJ44502.1    KU233524    ANZ02995.1    NWSH01001060    PCG72858.1    KQ459582    KPI98997.1    BABH01038087    BABH01038088    BABH01038089    BABH01038090    KC007356    AGQ45621.1    AGBW02008974    OWR52018.1    OWR52019.1    KQ460878    KPJ11641.1    JN033705    AEW46858.1    KY938815    ASA46456.1    GEYN01000016    JAV02113.1    AXCN02001099    KZ150230    PZC72001.1    AXCM01001195    GALX01004754    JAB63712.1    ODYU01009309    SOQ53638.1    EZ407153    ACX53715.1    MF687608    ATJ44534.1    ODYU01001496    SOQ37780.1    MF687572    ATJ44498.1    KPI96444.1    NWSH01005088    PCG64446.1    MF687575    ATJ44501.1    KC007350    AGQ45615.1    GBYB01000353    GBYB01000354    JAG70120.1    JAG70121.1    GALX01003699    JAB64767.1    KP237872    AKD01725.1    MF687615    ATJ44541.1    AGBW02010783    OWR47810.1    JN033699    JN033700    RSAL01000051    AEW46852.1    AEW46853.1    RVE50216.1    LJIJ01002106    ODM90218.1    APCN01001457    GGFJ01007115    MBW56256.1    ADMH02001680    ETN61451.1    KQ459602    KPI93169.1    ATLV01024323    KE525351    KFB51230.1    EZ407241    ACX53798.1    MF687617    ATJ44543.1    KK853419    KDR07716.1    GBYB01002216    JAG71983.1    GGFJ01007118    MBW56259.1    GGFM01004985    MBW25736.1    GGFM01005176    MBW25927.1    GGFM01004986    MBW25737.1    GAIX01005504    JAA87056.1    AAAB01008804    EAA03854.4    AGBW02012481    OWR44997.1    GGFK01009036    MBW42357.1    GGFK01009071    MBW42392.1    ADMH02001912    ETN60533.1    AB701383    GGFJ01007119    MBW56260.1    GGFJ01007363    MBW56504.1    GGFK01008948    MBW42269.1    CH902618    EDV39898.1    BABH01038066    LC375687    BBD35022.1    NNAY01003257    OXU19723.1    GL888207    EGI65077.1   

Proteomes

UP000005204    UP000053240    UP000007151    UP000218220    UP000053268    UP000037510    + More
UP000075884    UP000075886    UP000075883    UP000075920    UP000075900    UP000075880    UP000283053    UP000094527    UP000076408    UP000075901    UP000075840    UP000075903    UP000000673    UP000030765    UP000027135    UP000075882    UP000007062    UP000075902    UP000075885    UP000076407    UP000007801    UP000075881    UP000215335    UP000007755   

PRIDE

H9JJU9    H9JJU8    H9IVT6    H9IVR7    H9JTG9    H9IVS0    + More

Pfam

PF00248   Aldo_ket_red        + More
PF13516   LRR_6
PF12937   F-box-like

Interpro

IPR023210   NADP_OxRdtase_dom        + More
IPR036812   NADP_OxRdtase_dom_sf       
IPR020471   Aldo/keto_reductase       
IPR018170   Aldo/ket_reductase_CS       
IPR001611   Leu-rich_rpt       
IPR001810   F-box_dom       
IPR032675   LRR_dom_sf       
IPR006553   Leu-rich_rpt_Cys-con_subtyp       
IPR036691   Endo/exonu/phosph_ase_sf       

SUPFAM

SSF51430   SSF51430       

Gene 3D

3.20.20.100     3.80.10.10     3.60.10.10    

CDD

cd06660  Aldo_ket_red

ProteinModelPortal

A0A0H5AQ19   H9JJU9   H9JJU8   A0A291P0V2   A0A291P0V4   B8ZX07   + More
G9F9G1   I4DKC0   A0A0N1PGT3   A0A212ENH0   U5KCB5   A0A2A4J392   A0A2A4J1K6   A0A212ERG2   A0A194PV62   H9IVT6   I4DKQ3   A0A0L7KNM6   A0A2A4IW06   A0A194RJ39   A0A2A4K2R0   A0A291P0Y7   A0A1L8D6L9   Q9Y020   I4DP16   A0A2W1BLQ6   A0A291P0T7   A0A1B2G267   A0A2A4JMB2   A0A194Q090   H9IVR7   U5KCB8   A0A212FE89   A0A212FE35   A0A194R6Z8   G9F9G0   A0A182N3T0   A0A1Z2RRK0   A0A1L8D6N3   A0A182QZ12   A0A2W1BC51   A0A182M9D3   V5GQ24   A0A2H1WKT0   D2SNM4   A0A291P0Y9   A0A2H1VAA3   A0A291P0U2   A0A194PUL9   A0A2A4IYA3   A0A291P0S0   U5KBY4   A0A182WDU2   A0A0C9QAV8   V5GT06   A0A182RUK0   A0A0F6Q2W6   A0A291P0W7   A0A182IZ90   A0A212F244   G9F9F4   A0A1D2MB39   A0A182YBI3   A0A182SQ44   A0A182HW15   A0A182V431   A0A2M4BT41   W5JBU5   A0A194PKB3   A0A084WLY6   D2SNV7   A0A291P0X3   A0A067QJF4   A0A0C9R605   A0A2M4BT73   A0A2M3ZB15   A0A2M3ZBK2   A0A2M3ZB18   A0A182KKP6   S4P5J6   Q5TX68   A0A182TDQ5   A0A212EU63   A0A182PHB3   A0A2M4ANN0   A0A182WUG3   A0A2M4ANT1   W5JAV1   H9JTG9   A0A2M4BT68   A0A2M4BU17   A0A2M4ANA7   B3M7Z0   H9IVS0   A0A182JS79   A0A232EMX7   F4WLB4  

PDB

5AZ1     E-value=0,     Score=1895

KEGG

101740031    K00011 aldehyde reductase [EC:1.1.1.21] | (RefSeq) aldo-keto reductase AKR2E4-like

PATHWAY

00040    Pentose and glucuronate interconversions - Bombyx mori (domestic silkworm)
00051    Fructose and mannose metabolism - Bombyx mori (domestic silkworm)
00052    Galactose metabolism - Bombyx mori (domestic silkworm)
00561    Glycerolipid metabolism - Bombyx mori (domestic silkworm)
00790    Folate biosynthesis - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)

GO

GO:0016491   GO:0000166   GO:0008106   GO:0004032   GO:0005829   GO:0008202   GO:0006355   GO:0043565   GO:0005634   GO:0007275   GO:0004812   GO:0006418   GO:0004887   GO:0003707   GO:0016021   GO:0055114  

PANTHER

PTHR11732  

Length:

353

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.00178

Exp number, first 60 AAs:

0.00021

Total prob of N-in:

0.02793

outside

1  -  353

 

 

Pi

313.580317

Theta

178.344848

Tajima's D

2.692935

CLR

0.0592

CSRT

0.96105194740263

Interpretation

Uncertain