Gene

KWMTBOMO00938  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA009799

Annotation

PREDICTED:_aldo-keto_reductase_AKR2E4-like_isoform_X2_[Bombyx_mori]

Location

Bomo_Chr2(+):6833073-6847249      View in EpiBrowser

Full name

Aldo-keto reductase AKR2E4      

Alternative Name

3-dehydroecdysone reductase
Aldo-keto reductase 2E

Location in the cell

Cytoplasmic   Reliability : 3.55

CDS

ATGGCTAAGGTATCGATTCCAACGATTAAATTGAACAATGGAATCGAATTCCCGGCCCTCGGCTACGGGACCTGGCTAGGACTGAACGATAAGATGGAATTCGAAATCACGGACGCGCGTAAACTACAGGACGCATTATCGTACGCCATCGATATAGGTTATCGACATATCGACACGGCGCATCTCTACCGAGTCGAGCCGGAGGTCGGGGAAGTTATAAATAAAAAAATTAAGGACGGTGTTGTCAAACGCGAAGACATTTTTGTTACTACAAAGGTGTGGCACCACCACCACAAGCAAGCGGACGTCGAGGTGGCGGTGCGCGACTGCCTCAGGAGACTGAATCTGGAGTACGTGGACTTGGTATTGGTGCACTGGCCAATGTCGATCTCGCAAGAAGGGGTAGACGAAAATATCGACTACCTGGAGACGTGGAGAGGGTTTGAGGAGGTCCTCAAGAAGGGACTGACCAGGTCCATCGGCGTGTCCAACTTCAACATCGAACAGATGAAGAGGTTACTAGCCAACTGCAACGTGCCGCCCGCCGTCAACCAGATCGAGATCAACCTAAATCTCGGTCAGAAGGAATTGGTGGACTACTGTCAGTCCAAGGGCGTGGTGGTGGTCGCGTACTCGCCATTCGGAACGATGGTGCCGTCCAGAAACTACCCGGACAGTCCGGAGCCTAAGATGGACAATCCCACGATGCTCTCGATAGCGAAGAAACACGGGAAGACCGTCACTCAGATCGCCTTAAGATATTTGTACCAGCGCGGGATCGTGTCTATACCGAAGACTGTGACTAAGAGCAGAGTGCTGGAGAACGCCTCGATCTTCGACTTCCAGCTGGACGACGAGGACGTCGCCACCCTGGCGCAGTTCGACAACGGCTTCCGGACGATCAGGCCGCTGTTCTGGCAGCCCTACGAGAACTACCCCTTCGATGTGGTCCTTGGACAAGAGCACGAAGACATACCGATCTCTTTGCTCAAGTGGAAGAATGGAGTCAACGGCGACATCGAATAA

Protein

MAKVSIPTIKLNNGIEFPALGYGTWLGLNDKMEFEITDARKLQDALSYAIDIGYRHIDTAHLYRVEPEVGEVINKKIKDGVVKREDIFVTTKVWHHHHKQADVEVAVRDCLRRLNLEYVDLVLVHWPMSISQEGVDENIDYLETWRGFEEVLKKGLTRSIGVSNFNIEQMKRLLANCNVPPAVNQIEINLNLGQKELVDYCQSKGVVVVAYSPFGTMVPSRNYPDSPEPKMDNPTMLSIAKKHGKTVTQIALRYLYQRGIVSIPKTVTKSRVLENASIFDFQLDDEDVATLAQFDNGFRTIRPLFWQPYENYPFDVVLGQEHEDIPISLLKWKNGVNGDIE

Description

NADP-dependent oxidoreductase with high 3-dehydroecdysone reductase activity. May play a role in the regulation of molting. Has lower activity with phenylglyoxal and isatin (in vitro). Has no activity with NADH as cosubstrate. Has no activity with nitrobenzaldehyde and 3-hydroxybenzaldehyde.

Biophysicochemical Properties

0.0044 mM for 3-dehydroecdysone

Similarity

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Keywords

3D-structure   Complete proteome   Lipid metabolism   NADP   Oxidoreductase   Reference proteome   Steroid metabolism  

Feature

chain  Aldo-keto reductase AKR2E4

Uniprot

H9JJU8   H9JJU9   A0A0H5AQ19   B8ZX07   A0A291P0V2   A0A291P0V4   + More
G9F9G1   I4DKC0   A0A0N1PGT3   A0A212ENH0   U5KCB5   A0A194PV62   A0A2A4J392   A0A212ERG2   A0A2A4J1K6   H9IVT6   I4DKQ3   A0A291P0T7   A0A2A4JMB2   A0A1L8D6L9   A0A194RJ39   A0A2A4K2R0   Q9Y020   A0A2A4IW06   A0A291P0Y7   A0A1B2G267   A0A1L8D6N3   A0A1Z2RRK0   A0A2W1BLQ6   U5KCB8   A0A2W1BC51   G9F9G0   A0A194Q090   A0A194PKB3   I4DP16   D2SNM4   A0A2H1WKT0   A0A291P0X3   A0A2H1VAA3   U5KBY4   A0A212FE35   H9IVR7   A0A194R6Z8   A0A291P0W7   A0A212EU63   H9IVS0   A0A291P0U2   V5GQ24   A0A194PSF7   A0A291P0Y9   G9F9F4   A0A291P0S0   A0A0C9QAV8   A0A212FE89   A0A2A4IYA3   D2SNV7   G9F9F8   A0A232EMX7   A0A194PUL9   A0A067QJF4   A0A0F6Q2W6   A0A212FML6   F4WLB4   A0A0C9R605   J3JX35   A0A2H1WKV4   A0A1D2MB39   H9JTG9   G9F9F7   A0A0U4B4X4   A0A0L7QVJ2   A0A212F244   K7IXG3   A0A1W4X5Y5   V5GT06   A0A154PH71   A0A195CTD6   A0A182QZ12   A0A088AGI5   A0A1L8D6N6   E9J719   V4B296   A0A291P0Z6   A0A310SCS5   V4AZ56   N6UG67   A0A2A3EI70   V9IEZ4   A0A1I9WLL7   A0A2H1V815   A0A1L8E8F0   S4P5J6   A0A1L8E7Z6   A0A1L8E836   A0A291P0V1   A0A182M9D3  

EC Number

1.1.1.-

Pubmed

19121390    27103441    28986331    22651552    26354079    22118469    + More
24053512    10187849    28605547    28756777    20074338    28648823    24845553    21719571    22516182    27289101    24012638    26694822    20075255    21282665    23254933    23537049    27538518    23622113   

EMBL

BABH01017849    BABH01017850    BABH01017851    BABH01017852    LC012974    LC375688    + More
BAR91212.1    BBD35023.1    FM955425    CAW30924.1    MF687611    ATJ44537.1    MF687578    ATJ44504.1    JN033706    AEW46859.1    AK401738    BAM18360.1    KQ460280    KPJ16305.1    AGBW02013667    OWR43007.1    KC007346    AGQ45611.1    KQ459593    KPI96644.1    NWSH01003732    PCG65872.1    AGBW02013028    OWR44065.1    PCG65871.1    BABH01038068    BABH01038069    BABH01038070    AK401871    BAM18493.1    MF687576    ATJ44502.1    NWSH01001060    PCG72858.1    GEYN01000020    JAV02109.1    KQ460152    KPJ17355.1    NWSH01000243    PCG78063.1    AJ131966    CAB41997.1    NWSH01006101    PCG63656.1    MF687609    ATJ44535.1    KU233524    ANZ02995.1    GEYN01000016    JAV02113.1    KY938815    ASA46456.1    KZ150094    PZC73640.1    KC007356    AGQ45621.1    KZ150230    PZC72001.1    JN033705    AEW46858.1    KQ459582    KPI98997.1    KQ459602    KPI93169.1    AK403246    BAM19656.1    EZ407153    ACX53715.1    ODYU01009309    SOQ53638.1    MF687617    ATJ44543.1    ODYU01001496    SOQ37780.1    KC007350    AGQ45615.1    AGBW02008974    OWR52019.1    BABH01038087    BABH01038088    BABH01038089    BABH01038090    KQ460878    KPJ11641.1    MF687615    ATJ44541.1    AGBW02012481    OWR44997.1    BABH01038066    LC375687    BBD35022.1    MF687572    ATJ44498.1    GALX01004754    JAB63712.1    KPI96371.1    MF687608    ATJ44534.1    JN033699    JN033700    RSAL01000051    AEW46852.1    AEW46853.1    RVE50216.1    MF687575    ATJ44501.1    GBYB01000353    GBYB01000354    JAG70120.1    JAG70121.1    OWR52018.1    NWSH01005088    PCG64446.1    EZ407241    ACX53798.1    JN033703    AEW46856.1    NNAY01003257    OXU19723.1    KPI96444.1    KK853419    KDR07716.1    KP237872    AKD01725.1    AGBW02007651    OWR54929.1    GL888207    EGI65077.1    GBYB01002216    JAG71983.1    BT127803    AEE62765.1    SOQ53637.1    LJIJ01002106    ODM90218.1    AB701383    JN033702    AEW46855.1    KU218649    ALX00029.1    KQ414726    KOC62648.1    AGBW02010783    OWR47810.1    AAZX01000044    GALX01003699    JAB64767.1    KQ434902    KZC11162.1    KQ977349    KYN03394.1    AXCN02001099    GEYN01000021    JAV02108.1    GL768421    EFZ11357.1    KB200718    ESP00397.1    MF687618    ATJ44544.1    KQ777390    OAD52083.1    ESP00396.1    APGK01021961    APGK01021962    KB740392    ENN80755.1    KZ288235    PBC31398.1    JR040676    AEY59237.1    KU932401    APA34037.1    ODYU01001161    SOQ36985.1    GFDG01003937    JAV14862.1    GAIX01005504    JAA87056.1    GFDG01003939    JAV14860.1    GFDG01003938    JAV14861.1    MF687580    ATJ44506.1    AXCM01001195   

Proteomes

UP000005204    UP000053240    UP000007151    UP000053268    UP000218220    UP000283053    + More
UP000215335    UP000027135    UP000007755    UP000094527    UP000053825    UP000002358    UP000192223    UP000076502    UP000078542    UP000075886    UP000005203    UP000030746    UP000019118    UP000242457    UP000075883   

PRIDE

H9JJU8    H9JJU9    H9IVT6    H9IVR7    H9IVS0    H9JTG9    + More

Pfam

PF00248   Aldo_ket_red        + More
PF13516   LRR_6
PF12937   F-box-like

Interpro

IPR020471   Aldo/keto_reductase        + More
IPR018170   Aldo/ket_reductase_CS       
IPR036812   NADP_OxRdtase_dom_sf       
IPR023210   NADP_OxRdtase_dom       
IPR036691   Endo/exonu/phosph_ase_sf       
IPR001611   Leu-rich_rpt       
IPR001810   F-box_dom       
IPR032675   LRR_dom_sf       
IPR006553   Leu-rich_rpt_Cys-con_subtyp       
IPR011004   Trimer_LpxA-like_sf       

SUPFAM

SSF51430   SSF51430        + More
SSF51161   SSF51161       

Gene 3D

3.20.20.100     3.60.10.10     3.80.10.10    

CDD

cd06660  Aldo_ket_red

ProteinModelPortal

H9JJU8   H9JJU9   A0A0H5AQ19   B8ZX07   A0A291P0V2   A0A291P0V4   + More
G9F9G1   I4DKC0   A0A0N1PGT3   A0A212ENH0   U5KCB5   A0A194PV62   A0A2A4J392   A0A212ERG2   A0A2A4J1K6   H9IVT6   I4DKQ3   A0A291P0T7   A0A2A4JMB2   A0A1L8D6L9   A0A194RJ39   A0A2A4K2R0   Q9Y020   A0A2A4IW06   A0A291P0Y7   A0A1B2G267   A0A1L8D6N3   A0A1Z2RRK0   A0A2W1BLQ6   U5KCB8   A0A2W1BC51   G9F9G0   A0A194Q090   A0A194PKB3   I4DP16   D2SNM4   A0A2H1WKT0   A0A291P0X3   A0A2H1VAA3   U5KBY4   A0A212FE35   H9IVR7   A0A194R6Z8   A0A291P0W7   A0A212EU63   H9IVS0   A0A291P0U2   V5GQ24   A0A194PSF7   A0A291P0Y9   G9F9F4   A0A291P0S0   A0A0C9QAV8   A0A212FE89   A0A2A4IYA3   D2SNV7   G9F9F8   A0A232EMX7   A0A194PUL9   A0A067QJF4   A0A0F6Q2W6   A0A212FML6   F4WLB4   A0A0C9R605   J3JX35   A0A2H1WKV4   A0A1D2MB39   H9JTG9   G9F9F7   A0A0U4B4X4   A0A0L7QVJ2   A0A212F244   K7IXG3   A0A1W4X5Y5   V5GT06   A0A154PH71   A0A195CTD6   A0A182QZ12   A0A088AGI5   A0A1L8D6N6   E9J719   V4B296   A0A291P0Z6   A0A310SCS5   V4AZ56   N6UG67   A0A2A3EI70   V9IEZ4   A0A1I9WLL7   A0A2H1V815   A0A1L8E8F0   S4P5J6   A0A1L8E7Z6   A0A1L8E836   A0A291P0V1   A0A182M9D3  

PDB

5AZ1     E-value=1.40602e-160,     Score=1452

KEGG

101740161    K00011 aldehyde reductase [EC:1.1.1.21] | (RefSeq) aldo-keto reductase AKR2E4-like

PATHWAY

00040    Pentose and glucuronate interconversions - Bombyx mori (domestic silkworm)
00051    Fructose and mannose metabolism - Bombyx mori (domestic silkworm)
00052    Galactose metabolism - Bombyx mori (domestic silkworm)
00561    Glycerolipid metabolism - Bombyx mori (domestic silkworm)
00790    Folate biosynthesis - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)

GO

GO:0016491   GO:0000166   GO:0008202   GO:0006355   GO:0043565   GO:0005634   GO:0007275   GO:0004812   GO:0006418   GO:0004887   GO:0003707   GO:0016021   GO:0055114  

PANTHER

PTHR11732  

Length:

341

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.04657

Exp number, first 60 AAs:

0.04527

Total prob of N-in:

0.02783

outside

1  -  341

 

 

Pi

209.81011

Theta

141.221891

Tajima's D

1.177512

CLR

0.121065

CSRT

0.710514474276286

Interpretation

Uncertain