Gene

KWMTBOMO00901  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA009778

Annotation

PREDICTED:_LIM_and_SH3_domain_protein_Lasp_isoform_X4_[Papilio_polytes]

Location

Bomo_Chr2(+):5982005-6020973      View in EpiBrowser

Full name

Nebulette      

Alternative Name

Actin-binding Z-disk protein

Location in the cell

Nuclear   Reliability : 3.34

CDS

ATGGCCGAAACACCTGAATTGAAACGGATCGCCGAAAACACGAAACTACAGAGCAACGTGAAGTACCACGCCGACTTCGAGAGGAGTAAAGGGAAATTCACACAGGTGGCAGACGATCCTGAGACCTTAAGGATCAAAGCGAACACGAAGATCATAAGTAACGTGGCGTACCACGGCGATCTCGAGAAGAAAGCCCAGATGGAGAGGCAGAGGCAGATCAACGAAAACGGAGAAATAGTGGGTAGCAGTCATCAAACACAACATCAGCAGTCAAGGCAGATCGGTCGCGTAACCGATCTGGATCCGTTGGCCAATGAAGTGCAGAGACCGGCTAGCTCACAGAATCCGCAAGTGTACATAGCAATGTATGATTACGAAGCCAACGACAGTGATGAGGTGTCGTTCCGTGAAGGCGACCTCATAATGAATGTAAGTGCTATAGACGAGGGCTGGATGATGGGTCAGGTGGTACGCACCGGCATGGCGGGCATGCTGCCAGCCAACTACGTCGAGACCGCACCGGCGTCTGCATTACGGCAATACAATTGA

Protein

MAETPELKRIAENTKLQSNVKYHADFERSKGKFTQVADDPETLRIKANTKIISNVAYHGDLEKKAQMERQRQINENGEIVGSSHQTQHQQSRQIGRVTDLDPLANEVQRPASSQNPQVYIAMYDYEANDSDEVSFREGDLIMNVSAIDEGWMMGQVVRTGMAGMLPANYVETAPASALRQYN

Description

Binds to actin and plays an important role in the assembly of the Z-disk. May functionally link sarcomeric actin to the desmin intermediate filaments in the heart muscle sarcomeres (PubMed:27733623). Isoform 2 might play a role in the assembly of focal adhesion (PubMed:15004028).

Subunit

Isoform 2 interacts with ZYX (Zyxin) (PubMed:15004028). Interacts (via nebulin repeats 1-5) with DESM (via rod region) (PubMed:27733623).

Keywords

3D-structure   Actin-binding   Alternative splicing   Complete proteome   Cytoplasm   Methylation   Polymorphism   Reference proteome   Repeat   SH3 domain  

Feature

chain  Nebulette
splice variant  In isoform 2.
sequence variant  In dbSNP:rs75301590.

Uniprot

A0A1E1WJR4   J3JX15   V5G4T0   A0A0J7NKB5   D6WRW3   A0A1W4XP20   + More
A0A1W4XDQ4   A0A1Y1M1U2   A0A0Q9WT86   A0A0Q9XN10   A0A0K8TT92   A0A182MK35   M9NFH3   A0A1L8E5B0   A0A0R3P729   A0A1W7RAC5   A0A023GGL2   A0A224Z550   A0A1E1XL55   A0A131YRQ4   B7P377   A0A0B7BBX7   A0A091SZE2   A0A091QJ41   A0A146J2K6   A0A2I2UA04   A0A091TY58   A0A286ZNE6   B7ZB56   A0A341D4Q5   B4DH47   Q8N8M3   K9IIL1   A0A2K5ZHP0   A0A2R9CAM1   A0A287BFR4   A0A2I2ZD16   A0A2Y9KDC0   F1PQ88   A0A2U4B6T5   A0A340X7X9   A0A2Y9NYK7   A0A2U3VEE8   A0A384ARD3   A0A2K6FXT3   A0A287DEC2   O76041-2   A0A2K6BYR8   A0A2K6N6Q5   A0A2K5TRL9   A0A2I3FXH0   A0A2K5CX77   A0A2K6PT29   A0A2K5M6V8   A0A2J8RVW7   A0A2K5PCW0   A0A2K5J026   A0A2I3TGT5   U3C6Z0   A0A1D5RAK7   A0A1S3F0R1   A0A0P6K1Y3   A0A093ER58   A0A0A0AKK3   A0A2Y9RFS6   A0A091WCI3   A0A091V4G9   A0A087QUA3   A0A091PN56   A0A091J5N6   A0A093HUU3   A0A091F2D7   A0A094L6Q3   A0A093HMC4   A0A093I764   A0A091UUT3   A0A091QQ79  

Pubmed

22516182    23537049    18362917    19820115    28004739    17994087    + More
26369729    10731132    12537568    12537572    12537573    12537574    16110336    17569856    17569867    15632085    28797301    29209593    26830274    15632090    17975172    14702039    22722832    22398555    16341006    9733644    10470015    15004028    15164054    15489334    24129315    27733623    11140941    25362486    16136131    25243066    17431167    25319552   

EMBL

GDQN01003805    JAT87249.1    APGK01052202    BT127783    KB741211    KB632263    + More
AEE62745.1    ENN72763.1    ERL90897.1    GALX01003426    JAB65040.1    LBMM01003986    KMQ92925.1    KQ971351    EFA06590.1    GEZM01042528    JAV79794.1    CH940647    KRF84335.1    CH933809    KRG06318.1    GDAI01000026    JAI17577.1    AXCM01000274    AE014296    AFH04470.1    GFDF01000163    JAV13921.1    CH379070    KRT08830.1    GFAH01000323    JAV48066.1    GBBM01002302    JAC33116.1    GFPF01010134    MAA21280.1    GFAA01003501    JAT99933.1    GEDV01007761    JAP80796.1    ABJB010418755    ABJB010491676    ABJB010776635    ABJB010892119    DS626813    EEC01049.1    HACG01042740    CEK89605.1    KK493376    KFQ64233.1    KK799327    KFQ27570.1    LC133191    CH473990    BAU79360.1    EDL78760.1    AANG04002256    KK467227    KFQ82525.1    AEMK02000074    AK316521    BAH14892.1    AK294926    BAG58008.1    AK096540    BAC04812.1    GABZ01007428    JAA46097.1    AJFE02018725    AJFE02018726    AJFE02018727    AJFE02018728    AJFE02018729    AJFE02018730    AJFE02018731    AJFE02018732    CABD030069448    CABD030069449    CABD030069450    CABD030069451    CABD030069452    CABD030069453    CABD030069454    CABD030069455    CABD030069456    CABD030069457    AAEX03001209    AGTP01041780    AGTP01041781    AGTP01041782    AGTP01041783    AGTP01041784    AGTP01041785    AGTP01041786    AGTP01041787    AGTP01041788    AGTP01041789    Y16241    Y17673    AF047368    AJ580772    AK295186    EF445000    AL359175    AL731547    AL157398    AL158160    CH471072    BC110453    BC126132    BC126134    AQIA01070259    AQIA01070260    ADFV01176518    ADFV01176519    ADFV01176520    ADFV01176521    ADFV01176522    ADFV01176523    ADFV01176524    ADFV01176525    ADFV01176526    ADFV01176527    NDHI03003644    PNJ12654.1    AACZ04070778    AACZ04070779    AACZ04070780    AACZ04070781    NBAG03000250    PNI61173.1    GAMT01008489    GAMS01001923    GAMR01006240    GAMP01005070    JAB03372.1    JAB21213.1    JAB27692.1    JAB47685.1    JSUE03043003    JSUE03043004    JSUE03043005    JU320560    AFE64316.1    GEBF01006026    JAN97606.1    KK368813    KFV42438.1    KL872299    KGL95024.1    KK735254    KFR13377.1    KL410573    KFQ97268.1    KL225912    KFM04807.1    KK661803    KFQ08683.1    KK501476    KFP15005.1    KK397951    KFV58288.1    KK719443    KFO63426.1    KL276503    KFZ66500.1    KL206260    KFV80615.1    KK586680    KFV97462.1    KL410182    KFQ94476.1    KK704005    KFQ29114.1   

Proteomes

UP000019118    UP000030742    UP000036403    UP000007266    UP000192223    UP000008792    + More
UP000009192    UP000075883    UP000000803    UP000001819    UP000001555    UP000011712    UP000008227    UP000252040    UP000233140    UP000240080    UP000001519    UP000248482    UP000002254    UP000245320    UP000265300    UP000248483    UP000245340    UP000261681    UP000233160    UP000005215    UP000005640    UP000233120    UP000233180    UP000233100    UP000001073    UP000233020    UP000233200    UP000233060    UP000233040    UP000233080    UP000002277    UP000008225    UP000006718    UP000081671    UP000053858    UP000248480    UP000053605    UP000053283    UP000053286    UP000053119    UP000052976    UP000053584   

PRIDE

M9NFH3    B4DH47    Q8N8M3    O76041   

Pfam

PF00018   SH3_1        + More
PF00880   Nebulin
PF00412   LIM
PF14604   SH3_9

Interpro

IPR001452   SH3_domain        + More
IPR000900   Nebulin_repeat       
IPR001781   Znf_LIM       
IPR036028   SH3-like_dom_sf       
IPR035629   Nebulin_SH3       
IPR035631   Nebulette_SH3       

SUPFAM

SSF50044   SSF50044       

CDD

cd11933  SH3_Nebulin_C
cd11935  SH3_Nebulette_C

ProteinModelPortal

A0A1E1WJR4   J3JX15   V5G4T0   A0A0J7NKB5   D6WRW3   A0A1W4XP20   + More
A0A1W4XDQ4   A0A1Y1M1U2   A0A0Q9WT86   A0A0Q9XN10   A0A0K8TT92   A0A182MK35   M9NFH3   A0A1L8E5B0   A0A0R3P729   A0A1W7RAC5   A0A023GGL2   A0A224Z550   A0A1E1XL55   A0A131YRQ4   B7P377   A0A0B7BBX7   A0A091SZE2   A0A091QJ41   A0A146J2K6   A0A2I2UA04   A0A091TY58   A0A286ZNE6   B7ZB56   A0A341D4Q5   B4DH47   Q8N8M3   K9IIL1   A0A2K5ZHP0   A0A2R9CAM1   A0A287BFR4   A0A2I2ZD16   A0A2Y9KDC0   F1PQ88   A0A2U4B6T5   A0A340X7X9   A0A2Y9NYK7   A0A2U3VEE8   A0A384ARD3   A0A2K6FXT3   A0A287DEC2   O76041-2   A0A2K6BYR8   A0A2K6N6Q5   A0A2K5TRL9   A0A2I3FXH0   A0A2K5CX77   A0A2K6PT29   A0A2K5M6V8   A0A2J8RVW7   A0A2K5PCW0   A0A2K5J026   A0A2I3TGT5   U3C6Z0   A0A1D5RAK7   A0A1S3F0R1   A0A0P6K1Y3   A0A093ER58   A0A0A0AKK3   A0A2Y9RFS6   A0A091WCI3   A0A091V4G9   A0A087QUA3   A0A091PN56   A0A091J5N6   A0A093HUU3   A0A091F2D7   A0A094L6Q3   A0A093HMC4   A0A093I764   A0A091UUT3   A0A091QQ79  

PDB

1NEB     E-value=6.8715e-16,     Score=200

KEGG

100852388    no KO assigned | (RefSeq) lasp; LIM and SH3 domain protein Lasp

GO

GO:0046872   GO:0051015   GO:0005925   GO:0008092   GO:0030018   GO:0070062   GO:0071691   GO:0005523   GO:0031005   GO:0048747   GO:0031674   GO:0008307   GO:0001725   GO:0005515   GO:0005524   GO:0015074   GO:0003676   GO:0005737   GO:0006396   GO:0000166   GO:0004887   GO:0003707   GO:0016021  

Subcellular location

Cytoplasm  

Length:

182

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.0316

Exp number, first 60 AAs:

0

Total prob of N-in:

0.12483

outside

1  -  182

 

 

Pi

269.963633

Theta

179.662282

Tajima's D

1.968049

CLR

0.113997

CSRT

0.877706114694265

Interpretation

Uncertain