Gene

KWMTBOMO00891  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA009774

Annotation

TDR12_BOMMO_RecName:_Full%3DPutative_ATP-dependent_RNA_helicase_TDRD12;_short=BmTdrd12;_altname:_full=RNA_helicase-like_protein,Tudor_domain-containing_protein_12

Location

Bomo_Chr2(+):5819645-5827588      View in EpiBrowser

Full name

Putative ATP-dependent RNA helicase TDRD12      

Alternative Name

RNA helicase-like protein
Tudor domain-containing protein 12

Location in the cell

Nuclear   Reliability : 3.633

CDS

ATGGCATCAGACTATTATCAAGTTGAGATATTACATTACCTCAATCCGAATTTAATATGGGTTGAAGTTCTTAATTCTCCCAATGAAATCTCATTTGAACAGCTGGGTGTTTATGGGATTTTGCCTATCGATGCATCTTTGGACGTTGAAAGACCTGGGTTAAAGCTTCAACGTTCAGAAGATTGGATGCCGGCAACAGCCATTCTGATGAAAAATATATTTCAGAACCTTGAACAAGTTTGGTTTTCACCTACACACATTGACCGCAGGTCATCAATATTTGACAATAACATACATAAATATGGTGAACTAATAATTAAAAAAAACGGTGTCCAATTGTATTTATCAAAAGAACTAGTCAAGGCGGGATTAGCTACCGAGGATCCATGTCAATTTCACCAGTATATGAGTTTAGGCAAAATAAAAACTAAGCTCAGTAATACCGAAACAAGAGCAGTTATAAAGAACTTGGAGGAATACTACAGGAAATCTAGTAAACCCAAAGAGCTATGGCAGAAATCTGTGCATCAAAACACTAGTATTTTCCATGCAGGTGAGCGACTGCAAGCTTTGACAGTAAAAAATCTGGAAAGACATAATAACAGACAGAATATTATGTTGTTAGAGAATAAGCTCAAAGATCTAGAGCAGTGCAAAGGTAGCGATGAAGTGTCCCTGGGTCGAGGCGTTTGTAGAGTACCATCAAACAAGTCAGAGATGGTCATGTTAACCAACAAGAGATTGAAAAATAGGCTTGAATTATTGTCTAAAATAAATATGAAATCAGATGCTACTGATGCTGTAAAATCGACTAAGAGAAACTTCAGTGGTGATGGACAAAGAAAAAACTTTGAAAATGATTTTGAATCTGATGATGAAAGTGTTAAAAAAGTTTCAATTGCGAATACCATCAATACTTCTGATGGATCAGCTAATGTCGTAGACAAACTTTTGGACGAAAAACAGATTGATAATGTTTTTAATAATAAAAAACAAATCTGCTATACAGAAAGTACTAGGAGAAACCCCGTGAAGAAAGCAGCTTGCATAGTATACGGGCCACCCAGCATCAATATCGATAAACTGCCGTTGAAAGAAGCTCCCAAAATGACTAAGACTGTGAAATGGACTCCACATGTGGACTGCGATAAAGAAGCTTCAGAAGTGTCGTTCGGTGACGTAGATTCTCATGTTAAATTAGATGTTAAGAATTTAGATAAGTTCCACGAAATAGCAGATAGAATAGAAATAGAAAAGACGATACCGGTAGACGTAAATATTCATAAAGATCTATATGATAGTATGATTAACAACAAGAATGAAAGTGAATCTAAAATAATGGAAACAGCTAATTTAAAAACCGAAATGAAGAATCTACGTAAATCAAGTATCCTACAGAGCAAGCTGAAACAGTTTGACAAATTTAATGTTTCCTCAAATTCTGCCGCGTCCGAATCTAGCACGAAATCCAGTATGGATTCCAGTCGTATATCAGATGAGGACGATCTCAGTTCCGATGATGAAATGTCTGAGATTATGGAAACTTTTAAACTAAATTTAGCTACACCTAAGAAATCTGAAGCAAAGCACACTATTGATCATATTGAAGTAAATAACACGAAATTAAACGCGAACCCTTTTAAAAATCTAGACGGAAGTAAATCAGTGTTTGTTGATAAACTAACAAGTCCCGTATTGCTGGTGCACACGAAGCGTAATAATAAAGTACAACCATGCAGCCTCCTTAGGGATGTCCCCTTCGGTACCAGCATCCACGTGGTGCTCAGAAACATGGGCATAAAACACCCGACCAGGCTTCAAACTGTATCATGGGGCACGATTTTGCGCGGCCTCAGTACATTCCTCATCAGCCCACCACGAAGCGGAAAAACTATGGGCTATTTACCAGCGGTCTGTCGTCTAGTCAGGGATTTTCGAAAGGAATCACCGGATTCGTGTGGTCCTAAATGTATTATTGTTTGTGCCACATCAAAATCTGTATCCGAAGTCGAGAGGATCAGTAAAATGCTGTTAGGTCTTGAAGATAAAGTGTTTGCTTGTTACTCCGGGATGGATAATCTCAGTGTAACAACAGCATTGCTGAACGGTTGTGATCTATTGATATGTACGCCAAAGTCGATTGTGCGACTGCTTCAAAACGACCTTAGCGTCGATTTGAGGGATCTCACCACGTTCGTTGTTGATGATTGTGAAAGGATATCAGATGTATATTCGAACGAAGTGAAATATGTTTTGTACGAAATAAAAAATATGCTAAAGAACCGTGTTAACAAAGAGCTTAAAGTCCAAATCGTTGTGGCTTCGAGAATATGGTGCGACTTCTTGGAGCCGATCGTTTTGAAAGCCCCCGATTCTGTAGTTTGCATTGGTGCGTTCCAAGAACTTATATTATATTCGAAAATTAGTACGACTGTTGATTTTCTTAGACCTGAAAATAAAATCGCAAACGTGTTACAGTTCATTGACTCTGTACAGGGTCCCAAAAGAACGGTAGTCGTATGTCGCGCCGATAATGAAGTTAAAGCTGTAGAAAGCTCTTTACGATACAATAACCGTGTCGTTTTCGCTTGCGACAACACCATGAATATCCACGATTTGTACAATTTAAATGTAGTTTGGGGTGATTTCGAAGATCCAACGTTGGGTCCAATACTGGTCTGTTGCGATAGTAATTTGGTTCACTTGAACGTCACAGATGCCAGTTACCTGATTCATTACTCATTGCCGGCTCTCTTTTCGACATTCTGCAAGAGATTCTCCGTTTTAAATGATAATTATCCTTCTATTTTTAAAAACGAGTCCAGAGATTTAAAGGTTAAGGTGTTGATGGACGAATCTAACGTAGAACAATTGCCGAAAATCCTTAATTTCCTTAAACGTTGTACCGAAAACGTTCCTAAAATACTGGACGAAGTTTCGGAGAAGATATTAAATGAAAAGGACTTGGCAAAGGTCAAAGACTTGGTGCCTCTGTGCGATAATTTACTGTCATTGGGAATTTGTCCTGATACCTGGAATTGTACTGAGAGACATCGAATATTCAAAGAGTGTGACAGTCCAGCTGACTGGATACCGAAGAATGGAGTGGTCACTTTCCAAATTTTATATTTCCATTCAGCCGTCATGTACTCTGCTCGTTTACTGTCAAATACAGTGGACGGTGAAACAACAAAATACCCTCAGACCTACAGTACTCTATCCCTCAAAATGGGTATGTACTTCAGCAAGGAATCTAGCAGACGGCTGCATGGTATTCCAATGGTTGGCGATGTGTGTGCTGTATCAAAGAAGCAAAATTTCTTCATTCGATGTCAAGTAGTGAAGATTATAAGTTTTTATAAAAACGGAAATCCTAATTATGTAGTAATAAAGCTTATAGACGAAGAGAAGTTTGAGCAGAGTCGCGATATTTATTTATACCATTTACCCGACGAATTCAAAGACATGAAAACTTACGTTGTACAAGTCAGACTAGCTAATATACAACCACAGGACAAAGACATTACGTTTTCTTGTTTAGCTAAAAATGAGCTGGAGAAAATCGTTGAGAAGAACGAAGATTTGTTCATGAGAGGCCACGTGGCTATGAGTGTAGGAAGCTGTATATTTGTGGATACTTTAGAAGCTTGTCTGGACCTATCATCTCTCAGCGAAACTGTAGTAAGGCATAATTTCAAACAAGAACTATTAAACGCTCATGCTGTTCCTAATCCTAAACATTTAAGCATTTTAGAAGAAATGTGCGAAAAGTCTGGTTTGATTGTTAAAGCGGTGACAAACGAACAGGTAGTGCCAAAACCGATACCAGTACTACCAGCAGCGCAATGGGCGCACCTGGAAGATGATTTGTCGTCCGTATATTTGGCCTCAGTCGAAGATATGGATAAACTCTTTGTTCGTCTTGTGAAATTCGAAAGTTGCATGAAATTGTTGAACATCGAGATTAATAAATATGTGTCCGAAAACACCGTTCCGTTGGACGGGTCAAATGTTGGGGATATAGTACTGGCTAAGTTCCCTGATGATTCGATGTACGAGAGGGCAAGGATAGATCATATTTACTCAGAAGACAAAGTAAAATGCTTCTTCGTTGACCAGGGTGATTGGAGGGATGTCTCCACAAACGATCTAGCGACAATAACCGAGAATTTCATTACCCAACTACCATTCCAAGCGATTGAATGTAGATTAATAGGCATCAGACCTTTTGGGGAGCAATGGACGGAGTTCAGCACGAACTGGTTTAGCGACCATTGTTTTGAAGATGCTAAAGGCAATTTGAAACACCTTTACGTTAAGCATTTCACCAAGGAAAAAGCCGATTGTACTGGAGGTCACAAATATGGTGTTGCTTTAATAGACACATACACGAACGAAGACATTATAGTTAACCAGCTATTGATAGACTTGAATTTAGCCAAAGAAAACGTAGATGAAATAGCATATCTATCAGAAATCAAATGCAATAAAACGGTTTTAAATAATGACGCAACCGTTGATGAAGAAGAAGGTAGCTTATCAGGTGTTAGTGAACCCGAGAGTAACATAAACGTGCCCTTAGATAAGGTATTCTTAAAACCACCGATCCGCTCAGTGCCGCTAGTTGATTCCGATAACGAAACGAGTGATTCTGATACGTGGCAGATCAACAGGCCTGAAGATTTCAAAGCGCTTTTCATGAGGACCAGACCCGAGTCTTCGAAAATAATACCTATGATAACTGCCAATGAAGTACAAAACAATGCAGATGGAGAAACGTCAAAAGATACTTCGACTATATTAGAAGAAAAGGGTCAATTGCCAGAAAAAGTCAAGGACGATGAATTGAAATTGAGCAAACCGAAGATTTGTTGGAGCCAAAACAAAAATACGGTGAAATTGAAAATTTTGATTGCCGGAATCGAGGATTACAAGCTGAAAATCGAAGATCGAGCTGTCGCGTTCTCGGCCAATCACTGTGACGTCGAATATGGTTTCAAACTGGAACTGTACGGAGTCGTTGATGTTAACAAATCTAGACATTCGAACAAAGGGCAATACGTTTTGGTGACCATGACGAAATTGATGTGCAGAAATTGGCTGGCGTTGACCAAAGAGGGGGATTCCCAGAAGTGGATCGTGTACGATGTTGATACGATTGAGGCTTCGTCAGACGAAGAAGTGTATCGCGACGATACGTTAGAAGTGATTAAGAACATTCACAATACAAATAACGGATCTGACAGCGAAGACGATGATTTCTTGGATGATGTCAGTTAA

Protein

MASDYYQVEILHYLNPNLIWVEVLNSPNEISFEQLGVYGILPIDASLDVERPGLKLQRSEDWMPATAILMKNIFQNLEQVWFSPTHIDRRSSIFDNNIHKYGELIIKKNGVQLYLSKELVKAGLATEDPCQFHQYMSLGKIKTKLSNTETRAVIKNLEEYYRKSSKPKELWQKSVHQNTSIFHAGERLQALTVKNLERHNNRQNIMLLENKLKDLEQCKGSDEVSLGRGVCRVPSNKSEMVMLTNKRLKNRLELLSKINMKSDATDAVKSTKRNFSGDGQRKNFENDFESDDESVKKVSIANTINTSDGSANVVDKLLDEKQIDNVFNNKKQICYTESTRRNPVKKAACIVYGPPSINIDKLPLKEAPKMTKTVKWTPHVDCDKEASEVSFGDVDSHVKLDVKNLDKFHEIADRIEIEKTIPVDVNIHKDLYDSMINNKNESESKIMETANLKTEMKNLRKSSILQSKLKQFDKFNVSSNSAASESSTKSSMDSSRISDEDDLSSDDEMSEIMETFKLNLATPKKSEAKHTIDHIEVNNTKLNANPFKNLDGSKSVFVDKLTSPVLLVHTKRNNKVQPCSLLRDVPFGTSIHVVLRNMGIKHPTRLQTVSWGTILRGLSTFLISPPRSGKTMGYLPAVCRLVRDFRKESPDSCGPKCIIVCATSKSVSEVERISKMLLGLEDKVFACYSGMDNLSVTTALLNGCDLLICTPKSIVRLLQNDLSVDLRDLTTFVVDDCERISDVYSNEVKYVLYEIKNMLKNRVNKELKVQIVVASRIWCDFLEPIVLKAPDSVVCIGAFQELILYSKISTTVDFLRPENKIANVLQFIDSVQGPKRTVVVCRADNEVKAVESSLRYNNRVVFACDNTMNIHDLYNLNVVWGDFEDPTLGPILVCCDSNLVHLNVTDASYLIHYSLPALFSTFCKRFSVLNDNYPSIFKNESRDLKVKVLMDESNVEQLPKILNFLKRCTENVPKILDEVSEKILNEKDLAKVKDLVPLCDNLLSLGICPDTWNCTERHRIFKECDSPADWIPKNGVVTFQILYFHSAVMYSARLLSNTVDGETTKYPQTYSTLSLKMGMYFSKESSRRLHGIPMVGDVCAVSKKQNFFIRCQVVKIISFYKNGNPNYVVIKLIDEEKFEQSRDIYLYHLPDEFKDMKTYVVQVRLANIQPQDKDITFSCLAKNELEKIVEKNEDLFMRGHVAMSVGSCIFVDTLEACLDLSSLSETVVRHNFKQELLNAHAVPNPKHLSILEEMCEKSGLIVKAVTNEQVVPKPIPVLPAAQWAHLEDDLSSVYLASVEDMDKLFVRLVKFESCMKLLNIEINKYVSENTVPLDGSNVGDIVLAKFPDDSMYERARIDHIYSEDKVKCFFVDQGDWRDVSTNDLATITENFITQLPFQAIECRLIGIRPFGEQWTEFSTNWFSDHCFEDAKGNLKHLYVKHFTKEKADCTGGHKYGVALIDTYTNEDIIVNQLLIDLNLAKENVDEIAYLSEIKCNKTVLNNDATVDEEEGSLSGVSEPESNINVPLDKVFLKPPIRSVPLVDSDNETSDSDTWQINRPEDFKALFMRTRPESSKIIPMITANEVQNNADGETSKDTSTILEEKGQLPEKVKDDELKLSKPKICWSQNKNTVKLKILIAGIEDYKLKIEDRAVAFSANHCDVEYGFKLELYGVVDVNKSRHSNKGQYVLVTMTKLMCRNWLALTKEGDSQKWIVYDVDTIEASSDEEVYRDDTLEVIKNIHNTNNGSDSEDDDFLDDVS

Description

Probable ATP-binding RNA helicase required during spermatogenesis to repress transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons.

Catalytic Activity

ATP + H2O = ADP + H(+) + phosphate

Subunit

Interacts (via Tudor domain 2) with Siwi (PubMed:24067652). Component of the PET complex, at least composed of EXD1, SIWI, TDRD12 and piRNAs (PubMed:26669262).

Keywords

ATP-binding   Chromosome   Complete proteome   Cytoplasm   Developmental protein   Differentiation   Helicase   Hydrolase   Meiosis   Membrane   Nucleotide-binding   Nucleus   Reference proteome   Repeat   RNA-mediated gene silencing   Spermatogenesis  

Feature

chain  Putative ATP-dependent RNA helicase TDRD12

Uniprot

Q1XG89   A0A1P1EMV3   A0A194QZQ0   A0A194PUF0   A0A0P5TK01   A0A0P6AW46   + More

EC Number

3.6.4.13

Pubmed

24067652    19121390    17098166    26669262    26354079   

EMBL

AB252573    BAE93115.1    BABH01017725    KQ460890    KPJ11023.1    KQ459593    + More
KPI96603.1    GDIP01125683    JAL78031.1    GDIP01025696    JAM78019.1   

Proteomes

UP000005204    UP000053240    UP000053268   

PRIDE

Q1XG89   

Pfam

PF04969   CS        + More
PF00270   DEAD
PF00567   TUDOR

Interpro

IPR035437   SNase_OB-fold_sf        + More
IPR011545   DEAD/DEAH_box_helicase_dom       
IPR007052   CS_dom       
IPR001650   Helicase_C       
IPR008978   HSP20-like_chaperone       
IPR014001   Helicase_ATP-bd       
IPR002999   Tudor       
IPR027417   P-loop_NTPase       

SUPFAM

SSF52540   SSF52540        + More
SSF49764   SSF49764       

Gene 3D

2.40.50.90     2.60.40.790    

ProteinModelPortal

Q1XG89   A0A1P1EMV3   A0A194QZQ0   A0A194PUF0   A0A0P5TK01   A0A0P6AW46   + More

PDB

6B57     E-value=2.13018e-13,     Score=190

KEGG

692554    K18409 ATP-dependent RNA helicase TDRD12 [EC:3.6.4.13] | (RefSeq) TDRD12, Rhl; RNA helicase-like pro

GO

GO:0005524   GO:0031047   GO:0007283   GO:0004386   GO:0030154   GO:0003676   GO:1990923   GO:0043186   GO:0005694   GO:0007275   GO:0005829   GO:0051321   GO:0031965   GO:0015074   GO:0005737   GO:0006396   GO:0000166   GO:0004887   GO:0003707   GO:0016021  

Subcellular location

Chromosome   At 36 hours after oviposition, detected in the nucleus and the cytosol where it is associated with chromosomes and to the surface of the nuclear membrane (PubMed:17098166). Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis (PubMed:24067652).   With evidence from 5 publications.
Cytoplasm   At 36 hours after oviposition, detected in the nucleus and the cytosol where it is associated with chromosomes and to the surface of the nuclear membrane (PubMed:17098166). Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis (PubMed:24067652).   With evidence from 5 publications.
Cytosol   At 36 hours after oviposition, detected in the nucleus and the cytosol where it is associated with chromosomes and to the surface of the nuclear membrane (PubMed:17098166). Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis (PubMed:24067652).   With evidence from 5 publications.
Nucleus membrane   At 36 hours after oviposition, detected in the nucleus and the cytosol where it is associated with chromosomes and to the surface of the nuclear membrane (PubMed:17098166). Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis (PubMed:24067652).   With evidence from 5 publications.

Length:

1759

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.00375

Exp number, first 60 AAs:

0.00303

Total prob of N-in:

0.00017

outside

1  -  1759

 

 

Pi

275.497711

Theta

238.514127

Tajima's D

0.266658

CLR

1.839002

CSRT

0.44342782860857

Interpretation

Uncertain