Gene

KWMTBOMO00839  Validated by peptides from experiments

Annotation

RNA_binding_motif_protein_Y14_[Bombyx_mori]

Location

Bomo_Chr2(-):4110274-4114161      View in EpiBrowser

Full name

RNA-binding protein 8A      

Alternative Name

Protein tsunagi

Location in the cell

Cytoplasmic   Reliability : 1.839 Nuclear   Reliability : 2.33

CDS

ATGGCTGACGTGCTAGATATCGAAAATTCAGAAGAATTTGAAGTCGACGAAGATGGTGAACAGGGAATCGCTAGACTTAAGGAGAAGGCACGTAAGCGAAAAGGTCGTGGTTTTGGCAACGAAGCCGGTGGAGGCAGTGCTGCAGAACGGGGTAACCGCGGAAGATACGACAGTTTAGCTCCGGAAGGCGATAGCGGTACACCAGGACCTCAGAGATCAGTTGAAGGCTGGATCCTGTTTGTCAGTAATGTACATGAAGAAGCACAAGAAGAAGATATACAGAATCAATTTTCCGAGTTTGGTGAGATAAAGAATATTCATCTGAATCTGGACCGACGCACAGGCTTCCTCAAAGGCTACGCTCTAGTTGAGTACGAGACTTATAAACAAGCCGCTAGTGCACGTGAAGCATTGGATGGCGCAGATATACTCGGCCAAGCGATATCAGTTGATTGGTGCTTCGTCAAGGGACCCACTAAATCACATAAGAAGAGACGATAA

Protein

MADVLDIENSEEFEVDEDGEQGIARLKEKARKRKGRGFGNEAGGGSAAERGNRGRYDSLAPEGDSGTPGPQRSVEGWILFVSNVHEEAQEEDIQNQFSEFGEIKNIHLNLDRRTGFLKGYALVEYETYKQAASAREALDGADILGQAISVDWCFVKGPTKSHKKRR

Description

Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs.
Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs (PubMed:14973490, PubMed:24967911). Involved in exon definition of genes containing long introns, including the rolled/MAPK gene (PubMed:20946982, PubMed:20946983). The mago-tsu heterodimer interacts with the EJC key regulator Pym leading to EJC disassembly in the cytoplasm (PubMed:24967911). Has a role in oskar mRNA localization to the posterior pole of the developing oocyte (PubMed:11691839).

Subunit

Heterodimer with MAGOH. Part of the mRNA splicing-dependent exon junction complex (EJC) complex; the core complex contains CASC3, EIF4A3, MAGOH and RBM8A.
Heterodimer (via RRM domain) with mago (PubMed:11691839, PubMed:12730685, PubMed:12704080). Part of the mRNA splicing-dependent exon junction complex (EJC) complex; the core complex contains btz/CASC3, eIF4AIII, mago and tsu/RBM8A (PubMed:14973490). Interacts with Pym (via N-terminus); the interaction is direct (PubMed:24967911, PubMed:14968132). Interacts with eIF4AIII (PubMed:14973490).

Miscellaneous

'Tsunagi' means 'connection' or 'link' in Japanese.

Similarity

Belongs to the RBM8A family.

Keywords

3D-structure   Complete proteome   Cytoplasm   Developmental protein   mRNA processing   mRNA splicing   mRNA transport   Nucleus   Reference proteome   RNA-binding   Transport  

Feature

chain  RNA-binding protein 8A

Uniprot

Q1HQB4   A0A194R0C8   A0A2A4J7A8   A0A2H1W6J9   G8A442   S4Q0B8   + More
I4DL10   A0A1E1W6X4   A0A212F6A2   A0A1E1W5J3   A0A182GH26   A0A182QXI2   A0A182NJA3   A0A182MMH8   A0A1I8JUP7   A0A182PEQ9   Q175A5   A0A182YR35   A0A195E3N0   A0A158P1E8   A0A2M3ZB47   A0A2M4AQL4   A0A2M4C1L7   W5JH19   A0A182FCB2   A0A151XH13   A0A1Q3F892   A0A182UND7   A0A182K4Q3   A0A182UL70   A0A182WTS0   A0A182LL96   Q7Q5L6   A0A182HI17   E1ZWQ4   A0A026X1P1   A0A182J1W8   A0A151X7G2   A0A0J7KJJ2   F4X0Y4   A0A151I000   A0A195EYB5   A0A151IFT1   B0WKA7   K7J3K6   E2C0P8   R4V253   A0A2J7R3F3   A0A1B6J7C5   A0A0L7QRF4   U5EYB1   E2BCV8   A0A1B6G3G0   A0A1B6DSP1   A0A067QXR0   A0A0K8TRK4   A0A232F6P0   A0A1B0DR84   A0A1L8DHX2   A0A1B6LEK0   A0A154P2G6   A0A084WEJ1   A0A1L8EDI0   A0A0M8ZUV4   B4J9I4   A0A0M3QUF4   T1PH98   A0A1I8PAK5   W8AXF2   A0A0A1XID3   Q28ZB8   B3MDP6   B4ME32   A0A2P8YS07   B4KQJ1   A0A3B0JGF2   B4P3X1   B3N7L5   F2FB57   Q9V535   A0A1W4UZM2   B4GHZ2   A0A0K8VHU1   A0A034VVB5   B4NN37   A0A182WCC1   B4HSM1   B4QGR9   A0A0L0CKR0   A0A1A9W0Y8   A0A1A9UU10   A0A1A9ZUX9   D3TKL1   A0A1A9XY30   A0A1B0BKU7   A0A2R5L8F6   M9MSS7   A0A336KNV2   A0A2P2HY58   A0A1J1I4D2  

Pubmed

26354079    21193013    23622113    22651552    22118469    26483478    + More
17510324    25244985    21347285    20920257    23761445    20966253    12364791    14747013    17210077    20798317    24508170    30249741    21719571    20075255    24845553    26369729    28648823    24438588    17994087    25315136    24495485    25830018    15632085    29403074    17550304    24416299    11691839    10731132    12537572    12537569    14973490    20946982    20946983    24967911    12730685    12704080    14968132    25348373    22936249    26108605    20353571   

EMBL

DQ443138    DQ497206    FJ602787    ABF51227.1    ABF55971.1    ACM24353.1    + More
KQ460890    KPJ10979.1    NWSH01002905    PCG67330.1    ODYU01006665    SOQ48710.1    GU108331    ADK56161.1    GAIX01000063    JAA92497.1    AK401978    KQ459593    BAM18600.1    KPI96557.1    GDQN01008331    GDQN01004536    GDQN01001903    JAT82723.1    JAT86518.1    JAT89151.1    AGBW02010065    OWR49266.1    GDQN01008797    GDQN01002866    JAT82257.1    JAT88188.1    JXUM01011038    KQ560322    KXJ83062.1    AXCN02000449    AXCM01001406    CH477404    EAT41626.1    KQ979709    KYN19479.1    ADTU01006397    GGFM01005016    MBW25767.1    GGFK01009749    MBW43070.1    GGFJ01010061    MBW59202.1    ADMH02001414    ETN62618.1    KQ982138    KYQ59605.1    GFDL01011273    JAV23772.1    AAAB01008960    EAA11513.3    APCN01005863    GL434886    EFN74382.1    KK107024    QOIP01000004    EZA62220.1    RLU23391.1    KQ982450    KYQ56264.1    LBMM01006642    KMQ90437.1    GL888505    EGI59886.1    KQ976633    KYM78876.1    KQ981928    KYN32879.1    KQ977771    KYN00006.1    DS231969    EDS29698.1    GL451846    EFN78442.1    KC740978    AGM32802.1    NEVH01007821    PNF35367.1    GECU01012636    JAS95070.1    KQ414784    KOC61081.1    GANO01000471    JAB59400.1    GL447410    EFN86470.1    GECZ01012805    JAS56964.1    GEDC01008649    JAS28649.1    KK852897    KDR14174.1    GDAI01000832    JAI16771.1    NNAY01000782    OXU26536.1    AJVK01008965    GFDF01008109    JAV05975.1    GEBQ01017855    JAT22122.1    KQ434796    KZC05534.1    ATLV01023219    KE525341    KFB48635.1    GFDG01002166    JAV16633.1    KQ435834    KOX71605.1    CH916367    EDW02491.1    CP012524    ALC40539.1    KA648054    AFP62683.1    GAMC01015718    JAB90837.1    GBXI01003575    JAD10717.1    CM000071    EAL25695.1    CH902619    EDV36431.1    CH940662    EDW58797.1    PYGN01000399    PSN47026.1    CH933808    EDW08160.1    OUUW01000001    SPP74430.1    CM000157    EDW89454.1    CH954177    EDV59420.1    BT126144    KF051019    KX531362    ADZ74171.1    AHX83805.1    ANY27172.1    AF173550    AE013599    AY071005    AAF58987.1    AAL29185.1    AAL48627.1    CH479183    EDW36112.1    GDHF01013886    JAI38428.1    GAKP01013182    JAC45770.1    CH964282    EDW85776.1    CH480816    EDW47048.1    CM000362    CM002911    EDX06294.1    KMY92433.1    JRES01000265    KNC32827.1    CCAG010004671    EZ421943    EZ424294    ADD18239.1    ADD20570.1    JXJN01016042    GGLE01001633    MBY05759.1    HM132050    AEC22816.1    UFQS01000305    UFQT01000305    SSX02680.1    SSX23054.1    IACF01000981    LAB66714.1    CVRI01000039    CRK94618.1   

Proteomes

UP000053240    UP000218220    UP000053268    UP000007151    UP000069940    UP000249989    + More
UP000075886    UP000075884    UP000075883    UP000075900    UP000075885    UP000008820    UP000076408    UP000078492    UP000005205    UP000000673    UP000069272    UP000075809    UP000075903    UP000075881    UP000075902    UP000076407    UP000075882    UP000007062    UP000075840    UP000000311    UP000053097    UP000279307    UP000075880    UP000036403    UP000007755    UP000078540    UP000078541    UP000078542    UP000002320    UP000002358    UP000008237    UP000235965    UP000053825    UP000027135    UP000215335    UP000092462    UP000076502    UP000030765    UP000053105    UP000001070    UP000092553    UP000095301    UP000095300    UP000001819    UP000007801    UP000008792    UP000245037    UP000009192    UP000268350    UP000002282    UP000008711    UP000000803    UP000192221    UP000008744    UP000007798    UP000075920    UP000001292    UP000000304    UP000037069    UP000091820    UP000078200    UP000092445    UP000092444    UP000092443    UP000092460    UP000183832   

PRIDE

Q1HQB4    F2FB57    Q9V535   

Pfam

PF00076   RRM_1        + More
PF05648   PEX11
PF00005   ABC_tran
PF00664   ABC_membrane
PF05090   VKG_Carbox

Interpro

IPR008111   RNA-bd_8        + More
IPR033744   RRM_RBM8       
IPR012677   Nucleotide-bd_a/b_plait_sf       
IPR000504   RRM_dom       
IPR035979   RBD_domain_sf       
IPR008733   PEX11       
IPR036640   ABC1_TM_sf       
IPR027417   P-loop_NTPase       
IPR011020   HTTM       
IPR011527   ABC1_TM_dom       
IPR007782   VKG_COase       
IPR017871   ABC_transporter_CS       
IPR039421   Type_I_exporter       
IPR003593   AAA+_ATPase       
IPR003439   ABC_transporter-like       

SUPFAM

SSF54928   SSF54928        + More
SSF52540   SSF52540       
SSF90123   SSF90123       

Gene 3D

3.30.70.330     1.20.1560.10    

CDD

cd12324  RRM_RBM8

ProteinModelPortal

Q1HQB4   A0A194R0C8   A0A2A4J7A8   A0A2H1W6J9   G8A442   S4Q0B8   + More
I4DL10   A0A1E1W6X4   A0A212F6A2   A0A1E1W5J3   A0A182GH26   A0A182QXI2   A0A182NJA3   A0A182MMH8   A0A1I8JUP7   A0A182PEQ9   Q175A5   A0A182YR35   A0A195E3N0   A0A158P1E8   A0A2M3ZB47   A0A2M4AQL4   A0A2M4C1L7   W5JH19   A0A182FCB2   A0A151XH13   A0A1Q3F892   A0A182UND7   A0A182K4Q3   A0A182UL70   A0A182WTS0   A0A182LL96   Q7Q5L6   A0A182HI17   E1ZWQ4   A0A026X1P1   A0A182J1W8   A0A151X7G2   A0A0J7KJJ2   F4X0Y4   A0A151I000   A0A195EYB5   A0A151IFT1   B0WKA7   K7J3K6   E2C0P8   R4V253   A0A2J7R3F3   A0A1B6J7C5   A0A0L7QRF4   U5EYB1   E2BCV8   A0A1B6G3G0   A0A1B6DSP1   A0A067QXR0   A0A0K8TRK4   A0A232F6P0   A0A1B0DR84   A0A1L8DHX2   A0A1B6LEK0   A0A154P2G6   A0A084WEJ1   A0A1L8EDI0   A0A0M8ZUV4   B4J9I4   A0A0M3QUF4   T1PH98   A0A1I8PAK5   W8AXF2   A0A0A1XID3   Q28ZB8   B3MDP6   B4ME32   A0A2P8YS07   B4KQJ1   A0A3B0JGF2   B4P3X1   B3N7L5   F2FB57   Q9V535   A0A1W4UZM2   B4GHZ2   A0A0K8VHU1   A0A034VVB5   B4NN37   A0A182WCC1   B4HSM1   B4QGR9   A0A0L0CKR0   A0A1A9W0Y8   A0A1A9UU10   A0A1A9ZUX9   D3TKL1   A0A1A9XY30   A0A1B0BKU7   A0A2R5L8F6   M9MSS7   A0A336KNV2   A0A2P2HY58   A0A1J1I4D2  

PDB

2X1G     E-value=2.36741e-52,     Score=514

KEGG

732878    K12876 RNA-binding protein 8A | (RefSeq) Y14; RNA binding motif protein

PATHWAY

03013    RNA transport - Bombyx mori (domestic silkworm)
03015    mRNA surveillance pathway - Bombyx mori (domestic silkworm)
03040    Spliceosome - Bombyx mori (domestic silkworm)

GO

GO:0008380   GO:0005737   GO:0051028   GO:0003729   GO:0016607   GO:0006397   GO:0000184   GO:0035145   GO:0000381   GO:0000226   GO:0007317   GO:0051663   GO:0007294   GO:0000335   GO:0007314   GO:0005779   GO:0016559   GO:0071013   GO:0005634   GO:0045451   GO:0046595   GO:0000398   GO:0007310   GO:0008488   GO:0005524   GO:0042626   GO:0017187   GO:0016021   GO:0003676   GO:0003723   GO:0006396   GO:0000166   GO:0004887   GO:0003707  

PANTHER

PTHR24221  

Subcellular location

Nucleus  
Nucleus speckle  
Cytoplasm  

Length:

166

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.00135

Exp number, first 60 AAs:

0

Total prob of N-in:

0.22679

outside

1  -  166

 

 

Pi

291.002789

Theta

170.11937

Tajima's D

2.289611

CLR

0.213658

CSRT

0.91870406479676

Interpretation

Uncertain