Gene

KWMTBOMO00788

Pre Gene Modal

BGIBMGA009702

Annotation

acetylglucosaminyltransferase_[Bombyx_mori]

Location

Bomo_Chr2(-):2983846-2999164      View in EpiBrowser

Full name

Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase      

Alternative Name

N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I

Location in the cell

Mitochondrial   Reliability : 1.775 Nuclear   Reliability : 1.296

CDS

ATGCGCCTGAATGTAAGAAAACTATTGTTTGTTAGTTGCGGTGTTTTATTGTTGTGGTTCATCGTCTCTTACTCGTCGATTATCGAGTTGCCGAGGTCTATAACCTCTAAGGACACAGCAGCCGACGTAGAAAACAAATTAGGCAGACTTCAGAAGAAAATTGATGACCAAATGCTGGAAAGCAAAACACTGTTGCAGAAAGTGAAAAATGAATTGAAAAAAAAAACGCAATCAAAGGAGTCTTTAATTCAGTACTCTGATGAAATTATTGATGGTCCAACAATACTACCGGTGCTTGTGATCGCTTGCGACCGGGTCACAGTTAAAAGGTGTCTCGACAATCTAGTCAAGTTCAGACCGGACAAGGAAAGGTTTCCGATCGTAGTAAGCCAAGATTGCGGTCACAACGCCACTTACCAAGTGATACGCAGCTTCACCGATGCGGATCCGTCGATAAAGGTCATACTGCAACCGGATTTGTCCGAGATACCGCTTCCGAAGGTCAAGGTCAAGCTGAAAGGCTACTACAAGATCGCAAGGCATTTCAAATTCGCCTTAAATCACATGTTCAATACGTTGAATCACGAGGCTGTGATAATTGTTGAGGATGACCTGGACATTTCGCCGGATTTCTACGAATATTTCTTGGGCACGTATCCTTTACTGCAAAAGGATCCTAGTTTATGGTGCATATCGGCGTGGAATGACAACGGGAAGAAAGACATCATAGACCTGACCAGGCCGGAACTGTTGTACAGGACGGACTTCTTCCCGGGCCTCGGGTGGATGCTCAGGAAGGAGATGTGGACCAGACTCGAGCCGATATGGCCTCCGGCATTCTTCGACGACTGGCTCCGCAATCCAGTCAACACCCAAGGTCGAGCCTGCATCAGACCGGAGATATCCAGGACGTATTCGTTTGGGAAGATCGGCGTGAGTCAAGGACTGTTCTTCGATAAGCACCTTAGGAAAATACAATTGAATCTGGATTACGTCGAGTTCACTAAATTAAACTTAACTTATTTATTAAAGGATAATTACGACGAGACTCTAACCTCTACGGTGTACTCCTTGCCGGAGGTGATGGCCGAGGATGTGATACTTGGCGAGATGGACTCCGCAGTAAGGGTCTCTTACTCCAATGCCAAGAGCTACCAGCGGGCCGCCAAGAAACTTGGCCTCATGGACGACTTTAGAAGCGGCGTCCCCAGGACCGCGTACAGAGGTATAGTGACGTGCTTTATTCGAGGTCGACGTGTTTATCTGGCCCCGGACTACCAGTGGACCAAGTACGACCCCTCGTGGGGCTAG

Protein

MRLNVRKLLFVSCGVLLLWFIVSYSSIIELPRSITSKDTAADVENKLGRLQKKIDDQMLESKTLLQKVKNELKKKTQSKESLIQYSDEIIDGPTILPVLVIACDRVTVKRCLDNLVKFRPDKERFPIVVSQDCGHNATYQVIRSFTDADPSIKVILQPDLSEIPLPKVKVKLKGYYKIARHFKFALNHMFNTLNHEAVIIVEDDLDISPDFYEYFLGTYPLLQKDPSLWCISAWNDNGKKDIIDLTRPELLYRTDFFPGLGWMLRKEMWTRLEPIWPPAFFDDWLRNPVNTQGRACIRPEISRTYSFGKIGVSQGLFFDKHLRKIQLNLDYVEFTKLNLTYLLKDNYDETLTSTVYSLPEVMAEDVILGEMDSAVRVSYSNAKSYQRAAKKLGLMDDFRSGVPRTAYRGIVTCFIRGRRVYLAPDYQWTKYDPSWG

Description

Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans.

Catalytic Activity

N(4)-(alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (isomer 5A1,2) + UDP-N-acetyl-alpha-D-glucosamine = H(+) + N(4)-{beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + UDP

Cofactor

Mn(2+)

Subunit

Interacts with MGAT4D.

Similarity

Belongs to the glycosyltransferase 13 family.

Keywords

Complete proteome   Disulfide bond   Glycosyltransferase   Golgi apparatus   Manganese   Membrane   Metal-binding   Reference proteome   Signal-anchor   Transferase   Transmembrane   Transmembrane helix   Polymorphism  

Feature

chain  Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
sequence variant  In dbSNP:rs7726005.

Uniprot

F8WLR8   H9JJK1   H2BEB9   A0A194R0U9   A0A194PT25   A0A2H1VI14   + More
A0A212EX38   F7EGJ8   H3AN27   A0A2K6NLC0   A0A2J7PD74   A0A067QSE6   N6T3H9   Q924C0   P70680   Q924B9   U4UI41   V8NFG1   A0A1W7RJT8   Q924B8   Q544F0   P27808   A0A098M1Y9   J3S955   A0A1S3D9R7   Q8CIC9   T1E6R7   G3HK16   Q09325   I3N332   Q78E58   A0A087TMU3   A0A3B3WR82   A0A023F2K8   A0A3B4D982   A0A2A3EL29   A0A224XCI7   A0A0B8RV60   A0A0K8S2V2   A0A1A9ZYC3   A0A087X9E1   A0A1Y1L8B4   A0A1B0G3G9   A0A0L7R0U9   A0A315VXC4   A0A2I0T0W5   A0A1B0AXC5   A0A3B3VNM7   I3JAS7   A0A151M5X7   A0A3P8YP14   A0A1A9XVF6   A0A3B5L1Z1   A0A1U7SJ06   A0A2D0SWU1   A0A0A9W2X8   L5JVU8   A0A383ZEF0   G1KL90   A0A0A9X981   W5UBY9   A0A1I8MXJ3   A0A0L0BTQ1   A0A2F0B5I0   E2BDY8   A0A232EL13   A0A087ZQX0   A0A3B1JRJ3   A0A2K5XAV2   A0A2K5L3S5   Q0KKC2   A0A2J8JTB6   A0A091UZ47   A0A2R8Z919   K7CRV2   M4AFU5   A0A2K6AUI7   B3NJQ5   K7BGV7   A0A195D0W8   G7MUK4   A0A0M8ZVJ3   A0A1W4V4T8   G3R2D6   A0A310SC60   A0A3B5A790   K7J882   A0A158NYY3   A0A1A9V2E7   Q59G70   A0A3B0J032   A0A026W650   B4LIP0   A0A3P9N5Y9   P26572   A0A1I8P9G2   A0A091GC10   D2I5F9   B3KQ18  

EC Number

2.4.1.101

Pubmed

19121390    22238347    26354079    22118469    17495919    9215903    + More
25362486    24845553    23537049    11467936    8910379    24297900    26358130    10349636    11042159    11076861    11217851    12466851    12040188    16141073    21183079    1533386    1421759    16141072    15489334    20805325    23025625    23758969    25727380    21804562    7764514    25474469    25476704    28004739    29703783    25186727    22293439    25069045    25401762    26823975    23258410    23127152    25315136    26108605    20798317    28648823    25329095    16136131    22722832    23542700    17994087    17431167    22002653    25319552    22398555    20075255    21347285    24508170    30249741    1702225    1827260    14702039    15372022    20378551    24275569    20010809   

EMBL

AB587677    BAK55694.1    BABH01017414    BABH01017415    HQ888863    AEX00082.1    + More
KQ460890    KPJ10875.1    KQ459593    KPI96467.1    ODYU01002648    SOQ40431.1    AGBW02011830    OWR46062.1    AFYH01090827    NEVH01026393    PNF14283.1    KK853011    KDR12543.1    APGK01045744    APGK01045745    APGK01045746    KB741037    ENN74704.1    AF343963    AAK61868.1    U65791    AAC52872.1    AF343964    AAK61869.1    KB632173    ERL89550.1    AZIM01004468    ETE60706.1    GDAY02000016    JAV51397.1    AF343965    AAK61870.1    AK004760    AK040121    AK155322    CH466575    BAB23541.1    BAC30515.1    BAE33189.1    EDL33761.1    M73491    L07037    AK087959    BC006629    GBSI01000109    JAC96386.1    JU175199    GBEX01000024    AFJ50723.1    JAI14536.1    BC031752    AAH31752.1    GAAZ01000010    GBKC01000036    JAA97933.1    JAG46034.1    JH000448    EGV96592.1    D16302    BC074010    AGTP01110808    AGTP01110809    U65792    AAC52873.1    KK115950    KFM66432.1    GBBI01003162    JAC15550.1    KZ288226    PBC31896.1    GFTR01006280    JAW10146.1    GBSH01000148    JAG68876.1    GBKD01000011    JAG47607.1    AYCK01017180    GEZM01063649    JAV69041.1    CCAG010013348    KQ414670    KOC64421.1    NHOQ01000885    PWA28221.1    KZ527314    PKU27437.1    JXJN01005221    AERX01051254    AKHW03006526    KYO19830.1    GBHO01041828    GBHO01037557    GBHO01037554    GBHO01037550    GBRD01008547    GBRD01003590    GDHC01001738    JAG01776.1    JAG06047.1    JAG06050.1    JAG06054.1    JAG57274.1    JAQ16891.1    KB031114    ELK03187.1    GBHO01041835    GBHO01041831    GBHO01029979    GBHO01029978    GBRD01008548    GBRD01003589    GDHC01018788    JAG01769.1    JAG01773.1    JAG13625.1    JAG13626.1    JAG57273.1    JAP99840.1    JT405492    AHH37207.1    JRES01001351    KNC23460.1    NTJE010077542    MBV98266.1    GL447693    EFN86114.1    NNAY01003677    OXU19011.1    AEMK02000010    AB251342    BAF03221.1    AACZ04045833    NBAG03000426    PNI26010.1    PNI26011.1    PNI26013.1    PNI26016.1    PNI26019.1    KL410349    KFQ96274.1    AJFE02018931    GABE01009438    GABE01009437    GABE01009436    GABE01009435    JAA35303.1    CH954179    EDV55324.1    GABC01005459    GABC01005458    GABC01005457    GABC01005456    GABF01005459    GABF01005458    GABF01005457    GABF01005456    GABD01000081    GABD01000080    GABD01000079    GABD01000078    JAA05882.1    JAA16686.1    JAA33019.1    KQ977004    KYN06501.1    JSUE03037723    JU334560    JU475645    JU475646    JU475647    JU475648    JV047094    JV047095    CM001258    AFE78313.1    AFH32449.1    AFI37165.1    EHH27126.1    KQ435826    KOX72005.1    CABD030042842    KQ769812    OAD52833.1    ADTU01004399    AB209239    BAD92476.1    OUUW01000001    SPP73847.1    KK107419    QOIP01000003    EZA51086.1    RLU24583.1    CH940648    EDW61393.1    M55621    M61829    AK092256    AK094130    AK290769    CR456861    AC022413    CH471165    BC003575    KL447932    KFO78846.1    ACTA01187874    GL194665    EFB19613.1    AK057194    BAG51880.1   

Proteomes

UP000005204    UP000053240    UP000053268    UP000007151    UP000002280    UP000008672    + More
UP000233200    UP000235965    UP000027135    UP000019118    UP000030742    UP000000589    UP000079169    UP000001075    UP000002494    UP000005215    UP000054359    UP000261480    UP000261440    UP000242457    UP000092445    UP000028760    UP000092444    UP000053825    UP000092460    UP000261500    UP000005207    UP000050525    UP000265140    UP000092443    UP000261380    UP000189705    UP000221080    UP000010552    UP000261681    UP000001646    UP000095301    UP000037069    UP000008237    UP000215335    UP000005203    UP000018467    UP000233140    UP000233060    UP000008227    UP000002277    UP000053283    UP000240080    UP000002852    UP000233120    UP000008711    UP000078542    UP000006718    UP000053105    UP000192221    UP000001519    UP000261400    UP000002358    UP000005205    UP000078200    UP000268350    UP000053097    UP000279307    UP000008792    UP000242638    UP000005640    UP000095300    UP000053760    UP000008912   

PRIDE

F8WLR8    H9JJK1    Q924C0    P70680    Q924B9    Q924B8    + More
P27808    G3HK16    Q09325    Q78E58    Q0KKC2    Q59G70    P26572    B3KQ18   

Pfam

PF03071   GNT-I

Interpro

IPR004139   Glyco_trans_13        + More
IPR029044   Nucleotide-diphossugar_trans       

SUPFAM

SSF53448   SSF53448       

Gene 3D

3.90.550.10    

ProteinModelPortal

F8WLR8   H9JJK1   H2BEB9   A0A194R0U9   A0A194PT25   A0A2H1VI14   + More
A0A212EX38   F7EGJ8   H3AN27   A0A2K6NLC0   A0A2J7PD74   A0A067QSE6   N6T3H9   Q924C0   P70680   Q924B9   U4UI41   V8NFG1   A0A1W7RJT8   Q924B8   Q544F0   P27808   A0A098M1Y9   J3S955   A0A1S3D9R7   Q8CIC9   T1E6R7   G3HK16   Q09325   I3N332   Q78E58   A0A087TMU3   A0A3B3WR82   A0A023F2K8   A0A3B4D982   A0A2A3EL29   A0A224XCI7   A0A0B8RV60   A0A0K8S2V2   A0A1A9ZYC3   A0A087X9E1   A0A1Y1L8B4   A0A1B0G3G9   A0A0L7R0U9   A0A315VXC4   A0A2I0T0W5   A0A1B0AXC5   A0A3B3VNM7   I3JAS7   A0A151M5X7   A0A3P8YP14   A0A1A9XVF6   A0A3B5L1Z1   A0A1U7SJ06   A0A2D0SWU1   A0A0A9W2X8   L5JVU8   A0A383ZEF0   G1KL90   A0A0A9X981   W5UBY9   A0A1I8MXJ3   A0A0L0BTQ1   A0A2F0B5I0   E2BDY8   A0A232EL13   A0A087ZQX0   A0A3B1JRJ3   A0A2K5XAV2   A0A2K5L3S5   Q0KKC2   A0A2J8JTB6   A0A091UZ47   A0A2R8Z919   K7CRV2   M4AFU5   A0A2K6AUI7   B3NJQ5   K7BGV7   A0A195D0W8   G7MUK4   A0A0M8ZVJ3   A0A1W4V4T8   G3R2D6   A0A310SC60   A0A3B5A790   K7J882   A0A158NYY3   A0A1A9V2E7   Q59G70   A0A3B0J032   A0A026W650   B4LIP0   A0A3P9N5Y9   P26572   A0A1I8P9G2   A0A091GC10   D2I5F9   B3KQ18  

PDB

1FOA     E-value=1.15443e-112,     Score=1040

KEGG

100653407    K00726 alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase [EC:2.4.1.101] | (Re

PATHWAY

00510    N-Glycan biosynthesis - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)

GO

GO:0008375   GO:0006486   GO:0005794   GO:0000139   GO:0030145   GO:0003827   GO:0016021   GO:0001701   GO:0018279   GO:0006049   GO:0006487   GO:0035284   GO:0016319   GO:0035010   GO:0008344   GO:0005797   GO:0008340   GO:1903561   GO:0016020   GO:0070062   GO:0003723   GO:0016070   GO:0006260   GO:0006418   GO:0005737   GO:0006559   GO:0004887  

Subcellular location

Golgi apparatus membrane  

Length:

436

Number of predicted TMHs:

1

Exp number of AAs in TMHs:

20.09929

Exp number, first 60 AAs:

20.0915

Total prob of N-in:

0.95798

POSSIBLE N-term signal

sequence

inside

1  -  8

TMhelix

9  -  28

outside

29  -  436

 

 

Pi

264.191679

Theta

167.728786

Tajima's D

3.020227

CLR

0.106247

CSRT

0.978651067446628

Interpretation

Uncertain