Gene

KWMTBOMO00750  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA013340

Annotation

dihydrofolate_reductase_[Bombyx_mori]

Location

Bomo_Chr2(+):2418446-2422290      View in EpiBrowser

Full name

Dihydrofolate reductase      

Location in the cell

Cytoplasmic   Reliability : 2.533

CDS

ATGTCTCGTACGCAACTGAATTTGATTGCGGCCGCGTGTGAGAATATGGGTATAGGTATAAACGGTACATTACCTTGGAAATTAAAAAAAGAAATGGCTTACTTCACGACGATGACAACTAGTGTCAGCGACAAGAAGAAAGTCAACGCAGTGATCATGGGTCGACGTACGTGGGACTGTATACCCATAAAATATAGACCGCTTAGTAATAGAATTAATATTGTACTAACTCATAATGTTGATTACGTGAAAAAACAAGTCCCAGAAGGAGTAATTGTTGTGCCAGGATTAGATGAGGCAATTGATCACATAGACAGTAGGTCAGATATAGAATCTACTTGGGTCATAGGTGGATCTTCAATTTATAAGGCTGCAATGGAGCATCCGAACTGTGGAAAAATATATCTAACGGAGATACAAAAGTCTTTCGACTGTGACACATTCTTTCCTGACATTAACAAGCAGCGATTCCATTTAGTGAATGAAGAAGAAGTACCAGGGGAGAAGCAGAGTGAAGGAGACATCAACTATTACTATAGGATTTACAAGAGATTATAA

Protein

MSRTQLNLIAAACENMGIGINGTLPWKLKKEMAYFTTMTTSVSDKKKVNAVIMGRRTWDCIPIKYRPLSNRINIVLTHNVDYVKKQVPEGVIVVPGLDEAIDHIDSRSDIESTWVIGGSSIYKAAMEHPNCGKIYLTEIQKSFDCDTFFPDINKQRFHLVNEEEVPGEKQSEGDINYYYRIYKRL

Description

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (By similarity).
Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.

Catalytic Activity

(6S)-5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + H(+) + NADPH

Similarity

Belongs to the dihydrofolate reductase family.

Keywords

Direct protein sequencing   NADP   One-carbon metabolism   Oxidoreductase   Complete proteome  

Feature

chain  Dihydrofolate reductase

Uniprot

H9JUX7   J7GQW2   S4PKE6   A0A2A4IZV5   Q9U8B8   A0A194R0W4   + More
A0A194PT41   A0A076FMK2   A0A2W1BBC6   A0A2H1W3Q6   I4DP20   A0A2A4JZT6   A0A0J7KB40   A0A0L7R6H6   F4X717   A0A026WER7   A0A151ING7   A0A158ND39   A0A151WSI4   A0A232EUN9   A0A293MYP8   A0A151I2B0   A0A2G8KVS0   K7IQX8   A0A0M9AAI7   A0A195DV39   A0A067R1L3   A0A195F7T6   A0A1B0D834   L7MJG0   A0A084W5M1   U5EJW5   A0A2A3EIV5   A0A1B0ES64   V5HNV0   A0A131Y2W5   A0A1W4XFZ2   A0A2M4CUL7   A0A1L8E031   A0A0K8RLJ1   A0A131XID4   A0A088AA35   A0A182F8V9   G9I0D7   A0A1S3HNN8   A0A182VSS4   A0A2S2P348   A0A0P4VLU6   R4G4F4   A0A1E1X3H8   A0A131YXX8   A0A182IS72   A0A1J1I9F6   Q8JKV3   A0A1B6JB80   C4WVU0   A0A1Q3FBT2   A0A131YCH8   A0A182N8W6   A0A2M3ZBL0   A0A182YN11   A0A146KKR4   A0A1W4UIX8   A0A0A9VQ48   E1ZW71   A0A1Q3F044   A0A0V0G6V5   B0X1Y0   A0A182RAF0   A0A1B6GE52   A0A2M4ATG3   A0A1S4FG94   A0A0U2URU6   A0A2H8TRR4   A0A182UIG4   A0A336LYG9   A0A154P5I3   A0A182UTP9   A0A0B6Z6C9   A0A182KJX3   Q7Q0L5   A0A182IGN5   A0A182QHF5   U5EV26   Q172I3   A0A182JXD7   A0A023FG61   W8BUN1   A0A023G7U0   E2BDW3   A0A069DPL3   A0A034WSW6   W4Y9Q8   V3ZI95   A0A0K8V3U9   A0A3B0JRJ1   A0A2H0L232   A0A023EJ23   P28019   A0A0A1WL31  

EC Number

1.5.1.3

Pubmed

19121390    23622113    10632709    26354079    28756777    22651552    + More
21719571    24508170    21347285    28648823    29023486    20075255    24845553    25576852    24438588    25765539    28049606    22355451    27129103    28503490    26830274    12186886    25244985    26823975    25401762    20798317    26621068    20966253    12364791    14747013    17210077    17510324    24495485    26334808    25348373    23254933    24945155    26483478    1915358    25830018   

EMBL

BABH01041437    BABH01041438    AB999997    BAS21454.1    JQ348842    AFP74113.1    + More
GAIX01004540    JAA88020.1    NWSH01004860    PCG64643.1    AF104106    AAF04459.1    KQ460890    KPJ10890.1    KQ459593    KPI96482.1    KJ803015    AII19520.1    KZ150197    PZC72318.1    ODYU01006001    SOQ47462.1    AK403254    BAM19660.1    NWSH01000338    PCG77286.1    LBMM01010424    KMQ87471.1    KQ414646    KOC66480.1    GL888828    EGI57779.1    KK107260    EZA54136.1    KQ976933    KYN07012.1    ADTU01012310    KQ982776    KYQ50751.1    NNAY01002107    OXU22061.1    GFWV01020323    MAA45051.1    KQ976547    KYM80934.1    MRZV01000345    PIK52062.1    KQ435700    KOX80540.1    KQ980341    KYN16429.1    KK853095    KDR11482.1    KQ981744    KYN36446.1    AJVK01036301    GACK01001611    JAA63423.1    ATLV01020673    KE525304    KFB45515.1    GANO01002059    JAB57812.1    KZ288249    PBC31119.1    AJWK01004413    GANP01007151    JAB77317.1    GEFM01003086    JAP72710.1    GGFL01004801    MBW68979.1    GFDF01002199    JAV11885.1    GADI01002055    JAA71753.1    GEFH01002711    JAP65870.1    JN418988    AEW69660.1    GGMR01011185    MBY23804.1    GDKW01001154    JAI55441.1    ACPB03020779    GAHY01001410    JAA76100.1    GFAC01005373    JAT93815.1    GEDV01004720    JAP83837.1    CVRI01000044    CRK96835.1    AF451898    AAN04305.1    GECU01011225    JAS96481.1    ABLF02029344    AK341669    BAH72010.1    GFDL01010073    JAV24972.1    GEDV01011950    JAP76607.1    GGFM01005141    MBW25892.1    GDHC01021561    JAP97067.1    GBHO01045685    GDHC01009896    JAF97918.1    JAQ08733.1    GL434776    EFN74580.1    GFDL01014124    JAV20921.1    GECL01002692    JAP03432.1    DS232274    EDS38910.1    GECZ01009051    JAS60718.1    GGFK01010547    MBW43868.1    KT754453    ALS04287.1    GFXV01000707    GFXV01005082    MBW12512.1    MBW16887.1    UFQS01000186    UFQT01000186    SSX00927.1    SSX21307.1    KQ434822    KZC07112.1    HACG01017057    CEK63922.1    AAAB01008980    EAA14339.3    APCN01005279    AXCN02001867    GANO01002058    JAB57813.1    CH477436    EAT40943.1    GBBK01003985    JAC20497.1    GAMC01001495    JAC05061.1    GBBM01005546    JAC29872.1    GL447689    EFN86169.1    GBGD01003124    JAC85765.1    GAKP01001238    GAKP01001236    JAC57714.1    AAGJ04143548    KB203566    ESO83922.1    GDHF01018712    JAI33602.1    OUUW01000007    SPP83623.1    PCVQ01000031    PIQ77595.1    GAPW01004799    KQ561085    JAC08799.1    KXJ79759.1    X60192    GBXI01015067    GBXI01013178    JAC99224.1    JAD01114.1   

Proteomes

UP000005204    UP000218220    UP000053240    UP000053268    UP000036403    UP000053825    + More
UP000007755    UP000053097    UP000078542    UP000005205    UP000075809    UP000215335    UP000078540    UP000230750    UP000002358    UP000053105    UP000078492    UP000027135    UP000078541    UP000092462    UP000030765    UP000242457    UP000092461    UP000192223    UP000005203    UP000069272    UP000029779    UP000085678    UP000075920    UP000015103    UP000075880    UP000183832    UP000232784    UP000007819    UP000075884    UP000076408    UP000192221    UP000000311    UP000002320    UP000075900    UP000075902    UP000076502    UP000075903    UP000075882    UP000007062    UP000075840    UP000075886    UP000008820    UP000075881    UP000008237    UP000007110    UP000030746    UP000268350    UP000249989    UP000069940   

PRIDE

H9JUX7    J7GQW2    Q9U8B8   

Pfam

PF00186   DHFR_1

Interpro

IPR024072   DHFR-like_dom_sf        + More
IPR017925   DHFR_CS       
IPR001796   DHFR_dom       
IPR012259   DHFR       

SUPFAM

SSF53597   SSF53597       

Gene 3D

3.40.430.10    

CDD

cd00209  DHFR

ProteinModelPortal

H9JUX7   J7GQW2   S4PKE6   A0A2A4IZV5   Q9U8B8   A0A194R0W4   + More
A0A194PT41   A0A076FMK2   A0A2W1BBC6   A0A2H1W3Q6   I4DP20   A0A2A4JZT6   A0A0J7KB40   A0A0L7R6H6   F4X717   A0A026WER7   A0A151ING7   A0A158ND39   A0A151WSI4   A0A232EUN9   A0A293MYP8   A0A151I2B0   A0A2G8KVS0   K7IQX8   A0A0M9AAI7   A0A195DV39   A0A067R1L3   A0A195F7T6   A0A1B0D834   L7MJG0   A0A084W5M1   U5EJW5   A0A2A3EIV5   A0A1B0ES64   V5HNV0   A0A131Y2W5   A0A1W4XFZ2   A0A2M4CUL7   A0A1L8E031   A0A0K8RLJ1   A0A131XID4   A0A088AA35   A0A182F8V9   G9I0D7   A0A1S3HNN8   A0A182VSS4   A0A2S2P348   A0A0P4VLU6   R4G4F4   A0A1E1X3H8   A0A131YXX8   A0A182IS72   A0A1J1I9F6   Q8JKV3   A0A1B6JB80   C4WVU0   A0A1Q3FBT2   A0A131YCH8   A0A182N8W6   A0A2M3ZBL0   A0A182YN11   A0A146KKR4   A0A1W4UIX8   A0A0A9VQ48   E1ZW71   A0A1Q3F044   A0A0V0G6V5   B0X1Y0   A0A182RAF0   A0A1B6GE52   A0A2M4ATG3   A0A1S4FG94   A0A0U2URU6   A0A2H8TRR4   A0A182UIG4   A0A336LYG9   A0A154P5I3   A0A182UTP9   A0A0B6Z6C9   A0A182KJX3   Q7Q0L5   A0A182IGN5   A0A182QHF5   U5EV26   Q172I3   A0A182JXD7   A0A023FG61   W8BUN1   A0A023G7U0   E2BDW3   A0A069DPL3   A0A034WSW6   W4Y9Q8   V3ZI95   A0A0K8V3U9   A0A3B0JRJ1   A0A2H0L232   A0A023EJ23   P28019   A0A0A1WL31  

PDB

8DFR     E-value=1.3813e-36,     Score=379

KEGG

101745190    K00287 dihydrofolate reductase [EC:1.5.1.3] | (RefSeq) dihydrofolate reductase-like

PATHWAY

00670    One carbon pool by folate - Bombyx mori (domestic silkworm)
00790    Folate biosynthesis - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)

GO

GO:0046654   GO:0050661   GO:0004146   GO:0006545   GO:0006730   GO:0016021   GO:0005739   GO:0046452   GO:0046655   GO:0016301   GO:0009165   GO:0005524   GO:0006418   GO:0005737   GO:0006559   GO:0004887   GO:0055114  

PANTHER

PTHR22778:SF16  

Length:

185

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.47708

Exp number, first 60 AAs:

0.47696

Total prob of N-in:

0.25820

outside

1  -  185

 

 

Pi

278.149216

Theta

175.314888

Tajima's D

1.85714

CLR

0

CSRT

0.857807109644518

Interpretation

Uncertain