Gene

KWMTBOMO00631

Pre Gene Modal

BGIBMGA003860

Annotation

PREDICTED:_nitrogen_permease_regulator_2-like_protein_isoform_X1_[Bombyx_mori]

Location

Bomo_Chr1(+):19597491-19614282      View in EpiBrowser

Full name

GATOR complex protein NPRL2      

Alternative Name

Nitrogen permease regulator 2-like protein

Location in the cell

Nuclear   Reliability : 2.643

CDS

ATGCAAGTACAGAGACGTTCGAAGGCATCGACGAGGAGGGACTTTGATGGTAGTTTAGAGCCGAATTTAGATTTGGTGTCTAGTAAAATAATGGAGACTAGATACTATGAAGGTTGCGGGCGGGAAGGCCCAATTCGATGTATATTCCTAGGGGAATTCCACCCTGTCGCAGGCCCAAAGATTTCATGCCAGTTCCCGGAAGACTATATTTCAAAAGAAGTTTTCGATTCAATAAGTGCATACATCATTCCTAAACCACAAATACAAAAATGCACTATGACCATAAATGCACTAGGGCACAAAATTGTTGGGTATCCAATTCGCATAGACAACCCCAGATATGAACGTAATGTGTACCTCTTCAATATCTGCTTTGTATGTGATAGTTGGTCCAAGACAGTGCAGTATGAGCCTGTTGTCAAAAAACTTGGTGAGCATTTGACAATAATGGAAGAAGAGACCGGGTTCGTATCTAGCGGGTCGTCCAAACTGCCCACTTTGCTGGCGCATCTTCTACACGATCTGAACACTCACAGGAAGGCAACCTTAGTTGAAGGAGACACAGTCATGCACCTCAAGGTCTTGGAGGTCCGCAAAGATCCACCTCCAGTTCACGACTACGACGTGCCCGTTTTGGTGGCGTCGGTGGGCCTACCCCGTCCTGGACGACCTCGCAGGCCTATCCCACCCGAGGGTACCGAGACACAGAGCCAGGAGCATTTGGATGTAGAAGATGACGAGCCTTTTGAAGTAGACATGGCTGCTGAATGGGACCTGACTACCAGACAGATTCTACCGTACATCAATAGCTACAACCACGTGTCCAAGATAGCGGCCGACACGAACGTCGAGAAGACCCTGGTTAAATCCTGCATTCAGAACTTGGTCTACTATGGAGTGGAATGA

Protein

MQVQRRSKASTRRDFDGSLEPNLDLVSSKIMETRYYEGCGREGPIRCIFLGEFHPVAGPKISCQFPEDYISKEVFDSISAYIIPKPQIQKCTMTINALGHKIVGYPIRIDNPRYERNVYLFNICFVCDSWSKTVQYEPVVKKLGEHLTIMEEETGFVSSGSSKLPTLLAHLLHDLNTHRKATLVEGDTVMHLKVLEVRKDPPPVHDYDVPVLVASVGLPRPGRPRRPIPPEGTETQSQEHLDVEDDEPFEVDMAAEWDLTTRQILPYINSYNHVSKIAADTNVEKTLVKSCIQNLVYYGVE

Description

An essential component of the GATOR subcomplex GATOR1 which functions as an inhibitor of the amino acid-sensing branch of the TORC1 signaling pathway (PubMed:23723238, PubMed:27166823, PubMed:25512509). The two GATOR subcomplexes, GATOR1 and GATOR2, regulate the TORC1 pathway in order to mediate metabolic homeostasis, female gametogenesis and the response to amino acid limitation and complete starvation (PubMed:23723238, PubMed:27166823, PubMed:25512509). The function of GATOR1 in negatively regulating the TORC1 pathway is essential for maintaining baseline levels of TORC1 activity under nutrient rich conditions, and for promoting survival during amino acid or complete starvation by inhibiting TORC1-dependent cell growth and promoting catabolic metabolism and autophagy (PubMed:23723238, PubMed:27166823). In addition, this inhibition of TORC1 is necessary to maintain female fertility under normal conditions and during periods of nutrient stress (PubMed:24786828, PubMed:27672113, PubMed:25512509). GATOR1 and GATOR2 act at different stages of oogenesis to regulate TORC1 in order to control meiotic entry and promote oocyte growth and development (PubMed:25512509). After exactly four mitotic cyst divisions, the GATOR1 complex members (Iml1, Nprl2 and Nprl3) down-regulate TORC1 to slow cellular metabolism and promote the mitotic/meiotic transition (PubMed:25512509). At later stages of oogenesis, the mio and Nup44A components of the GATOR2 complex inhibit GATOR1 and thus activate TORC1 to promote meiotic progression, and drive oocyte growth and development (PubMed:25512509).

Subunit

Component of the GATOR complex consisting of mio, Nup44A/Seh1, Im11, Nplr3, Nplr2, Wdr24, Wdr59 and Sec13 (PubMed:27166823). Within the GATOR complex, probable component of the GATOR1 subcomplex which is likely composed of Iml1, Nplr2 and Nplr3 (PubMed:27166823). Interacts with Nprl3 (PubMed:24786828).

Similarity

Belongs to the NPR2 family.

Keywords

Cell cycle   Cell division   Complete proteome   Cytoplasm   Lysosome   Meiosis   Mitosis   Reference proteome  

Feature

chain  GATOR complex protein NPRL2

Uniprot

A0A2A4IXB1   A0A2W1B3Q7   A0A212EQJ6   H9J2X3   A0A194QXV9   A0A2J7PXT5   + More
A0A182H4E0   A0A182H3M3   Q17EA9   A0A1S4G401   B0XL24   A0A1L8DF75   Q16E62   A0A067QLB4   A0A194PG93   A0A182QPI2   A0A1I8Q9U5   A0A1A9YF26   A0A1A9VUK7   A0A1B0AMR6   A0A1B0A0V7   A0A1B6CWT5   A0A1I8MUY4   A0A182VMS5   A0A182XDT4   Q7QFE3   A0A182IAS0   A0A182U1G1   A0A182XXU5   W8BQH1   A0A182LH02   A0A182MBW1   A0A182N1A1   A0A034WGG6   A0A0K8UFV4   A0A182P008   A0A182W5G9   A0A182RMW6   A0A0A1XIV2   A0A1B6MCH0   A0A1A9W648   A0A1B6GSQ5   A0A084VNV6   A0A182IWP1   A0A0A9Y5U1   A0A2M4ARG6   A0A2M4BQC9   A0A182FJA5   W5JRL9   A0A1B6J7B9   T1HAA8   B4M2A5   B4L1V0   B4NCB0   A0A3B0J7B3   Q29FL0   B4HA42   A0A0V0GBZ7   E0V9K4   K7ITL1   A0A224XDW6   A0A023F0H1   B4JL56   B4Q1W7   B4R647   Q9VXA0   A0A069DTW7   B3NW96   A0A151IFG8   E9ILD1   A0A0J7L4J3   B3N0C1   A0A1W4VIY6   B4IEU6   A0A151XAD9   F4WZK8   A0A310SKD5   A0A195DYG6   A0A195FFE7   A0A154PQN0   A0A1W4X2D4   A0A195B7V6   A0A158NGR1   A0A2A3EM38   A0A087ZVT4   A0A0L7QK55   E2BDB2   A0A0N0BEH8   E2ACA6   D6WVQ8   A0A1Y1N824   A0A026WPQ6   A0A0M3QYV4   A0A1Y1N970   A0A067R7Y0   A0A2J7QP81   A0A0T6B7V0   A0A3L8DMV7  

Pubmed

28756777    22118469    19121390    26354079    26483478    17510324    + More
24845553    25315136    12364791    14747013    17210077    25244985    24495485    20966253    25348373    25830018    24438588    25401762    26823975    20920257    23761445    17994087    15632085    20566863    20075255    25474469    17550304    10731132    12537572    12537569    23723238    24786828    25512509    27672113    27166823    26334808    21282665    21719571    21347285    20798317    18362917    19820115    28004739    24508170    30249741   

EMBL

NWSH01005590    PCG64116.1    KZ150373    PZC71129.1    AGBW02013268    OWR43773.1    + More
BABH01031241    KQ460953    KPJ10312.1    NEVH01020852    PNF21147.1    JXUM01109210    KQ565299    KXJ71113.1    JXUM01024583    KQ560695    KXJ81235.1    CH477284    EAT44769.1    DS234210    EDS33167.1    GFDF01009047    JAV05037.1    CH479067    EAT32519.1    KK853297    KDR08783.1    KQ459605    KPI92048.1    AXCN02001237    JXJN01000553    JXJN01000554    JXJN01000555    GEDC01019358    JAS17940.1    AAAB01008846    EAA06517.3    APCN01001313    GAMC01007492    JAB99063.1    AXCM01004309    GAKP01005748    GAKP01005747    JAC53205.1    GDHF01027109    JAI25205.1    GBXI01003415    JAD10877.1    GEBQ01006360    JAT33617.1    GECZ01004308    JAS65461.1    ATLV01014931    KE524996    KFB39650.1    GBHO01015172    GDHC01006029    JAG28432.1    JAQ12600.1    GGFK01010039    MBW43360.1    GGFJ01006129    MBW55270.1    ADMH02000670    ETN65389.1    GECU01012639    JAS95067.1    ACPB03021733    CH940651    EDW65809.1    CH933810    EDW06753.1    CH964239    EDW82469.1    OUUW01000003    SPP78124.1    CH379066    EAL31390.1    CH479235    EDW36710.1    GECL01000597    JAP05527.1    DS234994    EEB10060.1    GFTR01005781    JAW10645.1    GBBI01003762    JAC14950.1    CH916370    EDW00309.1    CM000162    EDX02542.1    CM000366    EDX18151.1    AE003503    AY051914    AAK93338.1    GBGD01001752    JAC87137.1    CH954180    EDV46435.1    KQ977793    KYM99659.1    GL764074    EFZ18599.1    LBMM01000666    KMQ97837.1    CH902640    EDV38325.1    CH480832    EDW46200.1    KQ982339    KYQ57322.1    GL888480    EGI60283.1    KQ763450    OAD55051.1    KQ980107    KYN17649.1    KQ981625    KYN39081.1    KQ435048    KZC14209.1    KQ976574    KYM80279.1    ADTU01015170    KZ288219    PBC32229.1    KQ415041    KOC58901.1    GL447585    EFN86340.1    KQ435833    KOX71614.1    GL438491    EFN68896.1    KQ971358    EFA08261.1    GEZM01013054    JAV92795.1    KK107135    EZA58042.1    CP012528    ALC48269.1    GEZM01013053    JAV92796.1    KK852819    KDR15634.1    NEVH01012097    PNF30389.1    LJIG01009518    KRT82919.1    QOIP01000006    RLU21711.1   

Proteomes

UP000218220    UP000007151    UP000005204    UP000053240    UP000235965    UP000069940    + More
UP000249989    UP000008820    UP000002320    UP000027135    UP000053268    UP000075886    UP000095300    UP000092443    UP000078200    UP000092460    UP000092445    UP000095301    UP000075903    UP000076407    UP000007062    UP000075840    UP000075902    UP000076408    UP000075882    UP000075883    UP000075884    UP000075885    UP000075920    UP000075900    UP000091820    UP000030765    UP000075880    UP000069272    UP000000673    UP000015103    UP000008792    UP000009192    UP000007798    UP000268350    UP000001819    UP000008744    UP000009046    UP000002358    UP000001070    UP000002282    UP000000304    UP000000803    UP000008711    UP000078542    UP000036403    UP000007801    UP000192221    UP000001292    UP000075809    UP000007755    UP000078492    UP000078541    UP000076502    UP000192223    UP000078540    UP000005205    UP000242457    UP000005203    UP000053825    UP000008237    UP000053105    UP000000311    UP000007266    UP000053097    UP000092553    UP000279307   

PRIDE

H9J2X3    Q9VXA0   

Pfam

PF06218   NPR2        + More
PF00620   RhoGAP
PF00788   RA

Interpro

IPR009348   NPR2        + More
IPR008936   Rho_GTPase_activation_prot       
IPR000198   RhoGAP_dom       
IPR029071   Ubiquitin-like_domsf       
IPR001849   PH_domain       
IPR000159   RA_dom       

SUPFAM

SSF48350   SSF48350        + More
SSF54236   SSF54236       

Gene 3D

1.10.555.10    

ProteinModelPortal

A0A2A4IXB1   A0A2W1B3Q7   A0A212EQJ6   H9J2X3   A0A194QXV9   A0A2J7PXT5   + More
A0A182H4E0   A0A182H3M3   Q17EA9   A0A1S4G401   B0XL24   A0A1L8DF75   Q16E62   A0A067QLB4   A0A194PG93   A0A182QPI2   A0A1I8Q9U5   A0A1A9YF26   A0A1A9VUK7   A0A1B0AMR6   A0A1B0A0V7   A0A1B6CWT5   A0A1I8MUY4   A0A182VMS5   A0A182XDT4   Q7QFE3   A0A182IAS0   A0A182U1G1   A0A182XXU5   W8BQH1   A0A182LH02   A0A182MBW1   A0A182N1A1   A0A034WGG6   A0A0K8UFV4   A0A182P008   A0A182W5G9   A0A182RMW6   A0A0A1XIV2   A0A1B6MCH0   A0A1A9W648   A0A1B6GSQ5   A0A084VNV6   A0A182IWP1   A0A0A9Y5U1   A0A2M4ARG6   A0A2M4BQC9   A0A182FJA5   W5JRL9   A0A1B6J7B9   T1HAA8   B4M2A5   B4L1V0   B4NCB0   A0A3B0J7B3   Q29FL0   B4HA42   A0A0V0GBZ7   E0V9K4   K7ITL1   A0A224XDW6   A0A023F0H1   B4JL56   B4Q1W7   B4R647   Q9VXA0   A0A069DTW7   B3NW96   A0A151IFG8   E9ILD1   A0A0J7L4J3   B3N0C1   A0A1W4VIY6   B4IEU6   A0A151XAD9   F4WZK8   A0A310SKD5   A0A195DYG6   A0A195FFE7   A0A154PQN0   A0A1W4X2D4   A0A195B7V6   A0A158NGR1   A0A2A3EM38   A0A087ZVT4   A0A0L7QK55   E2BDB2   A0A0N0BEH8   E2ACA6   D6WVQ8   A0A1Y1N824   A0A026WPQ6   A0A0M3QYV4   A0A1Y1N970   A0A067R7Y0   A0A2J7QP81   A0A0T6B7V0   A0A3L8DMV7  

PDB

6CET     E-value=1.94428e-43,     Score=441

KEGG

101743877    no KO assigned | (RefSeq) nitrogen permease regulator 2-like protein

GO

GO:0010508   GO:0006995   GO:1990130   GO:0034198   GO:0032007   GO:2000785   GO:0007165   GO:0005096   GO:0010898   GO:0051729   GO:1904262   GO:0045792   GO:0007293   GO:0044754   GO:0005634   GO:0035859   GO:1904766   GO:0051321   GO:0005737   GO:0048477   GO:0051301   GO:0038202  

PANTHER

PTHR12991  

Subcellular location

Cytoplasm   Localizes primarily to the autolysosomes during amino-acid starvation.   With evidence from 2 publications.
Lysosome   Localizes primarily to the autolysosomes during amino-acid starvation.   With evidence from 2 publications.

Length:

301

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.03824

Exp number, first 60 AAs:

0.00037

Total prob of N-in:

0.15914

outside

1  -  301

 

 

Pi

3.287691

Theta

2.56628

Tajima's D

-1.616776

CLR

0.093282

CSRT

0.0363981800909954

Interpretation

Uncertain