Silkworm Genome Informatics Database

Gene

KWMTBOMO00621

Pre Gene Modal

BGIBMGA003897

Annotation

PREDICTED:_E3_ubiquitin-protein_ligase_UHRF1-like_isoform_X2_[Bombyx_mori]

Location

Bomo_Chr1(-):19372572-19389948 View in EpiBrowser

Full name

E3 ubiquitin-protein ligase UHRF1

Alternative Name

Nuclear zinc finger protein Np95
RING-type E3 ubiquitin transferase UHRF1
Ubiquitin-like PHD and RING finger domain-containing protein 1
Ubiquitin-like-containing PHD and RING finger domains protein 1
Inverted CCAAT box-binding protein of 90 kDa
Nuclear protein 95
RING finger protein 106
Transcription factor ICBP90

Location in the cell

Nuclear Reliability : 4.493

CDS

ATGGTCAAAATAAACACAGATAGTCCAAAAAAGAGCCTCACGAAGTCTACAGCTGATGACAAAACTGACAGTGATAATAACAAAAATCATGAACAAGAATCTTCTGATGCTGTGTATACAGATGCAGTCAGCAAGCTCTATGCTGTTGGAGACTTGGTCGACGTAAGGGACCCAGAACACGGCGCTTGGTTCGAGAGTAAAATAATTAGAATAGTCCATGATCAGAAGGGAAAGAAATCGGAACATGAAAAAGAGAAGGGATCTGACACTTCTAGTTCTTATCAAAGTCAAAATTATGACTGCAATGACTTAGAGAACAAACCACCCATAGAAATGAATGGTGATTGTAAACCAAAGAGTAAAGGTATAGCTAAATACTTCACAAAAAGTCCTAAAGGAAAGAAAAAAGACAAAGATTCTAAGAGTGAGACCAGAATAGTTGATAACCTTCTTTATAAAGTGCAACTTGATAATGACGAAGACGACTCAGAATCTTACTGCAAAGAAAAAGACATAAGGCCACGAGCACGAACCATTATTGACATTAAGGATTTGAAAGTTGGACAAACAGTGATGGTGAATTACAATATTGAGGAGCCAATGGAAAAAGGTTATTGGTTCGATTTCAAAGTAAGTGAAATAAGGAAACTACGCACCAGCTATGATTTAGTGGGAACTTTATATTTGGGTCCCGAAGCAGTACCTCAGAATGACACCAAAATAAGATTGCAGGACAAAATTTTTGCCATAGAAGAAGTTGTACCACTGGAAAAGCGCAGTGATGAATACAATAGATTAATGACTATGAATCCTGAAAAACGGTCTCAACCATTAACTTGTTTGACTTGTCGTGATGATGAAGAAGCACTATGTAAAGACTGCGGTTGCTATGTCTGCCTAGGAAAAGAGTTTCCTGAAAAGATAGTGCTCTGTGACGAGTGCAACTACGGCTATCACATGAGCTGTCTCAAACCTCCATTAAAAGATCTTCCCGACGAGGACTGGTATTGCCCTTCTTGTAAAAGAGATCCTCAGGATGTAGTAGCGCCAGGAGCAGCCAAACAAACCAAGAAAAAAAATGCTTCACAGAGTAAACGCGATTGGGGCAGAGGAATGGCATGTGTTGGTAAGACGAAAACATGCGCTATGCCTGCCAACCATTTTGGACCGATCCCTGGCATCGAAGTTGGTATGTGTTGGCGGTTCAGGATTCAGTTATCCGAGTCTGGCGTGCATCGGCCGCCCGTGTCAGGAATCCACGGACGTGACGTAGAAGGCGCTTATAGCATTGTTTTATCCGGTGGATATGAAGATGACGTTGACAATGGCAATGAGTTCACGTACACGGGAAGCGGAGGTCGTGATCTATCCGGGAACAAGAGAACCGCCGAACAGTCGTGTGACCAGACCCTCACTAGAGAGAACAAGGCTTTGGCCAGGAACTGCGCTGTTAACCAAATCAAAGAAGACGGTGCTGACGCAGGTGAGAATTGGAAGAACGGGAAACCGGTCAGAGTTGTGAGATCCTACAAAATGATGAAGCACTTTCCGAAGTACGCGCCCAGCGAGGGGATCAGATACGACGGTATCTACAAAGTTGTAAAGTACTATCCAGAGAAGGGTCTGTCTGGCTACAGAGTATGGAAGTATTTACTGCGTAGAGACGATCCCAGTCCAGCGCCGTGGGAAGCTGGGGCTAAACATTATCCCATTGTGTATCCAGACGGTTATTTGGAAGCCGAGGCGGAGAAGAAAGCTTTGAAGCAGAAACAAAATACCAAATCGAAAGGAAAAGTGACAAAGAAGAGGGCGCTGCGAGAGAGCAACCAGTCTGCCGAGTCGGAGTGTGATATCTCCCCTCCCGCGAAGAAACGGAAAACCGCAAGCGAGAACAAGAGAAAAGGAAAATCATCAAAAACGAAATCGACGACAAGCCCAAATGGTAGTATTTTAGAAATGTTCTCACCGAAAAGAAGGAATTCTGCTAATGGGATGAAACAAGTTAAAAAATCGGAAAGTGTACTTACCAGCGAGGAAAGGGACGCAATTAAAGCTGACACGTTGAATAGCAAGTTATGGGGCGAATGTTTGGCTGTGTGCGATAGCTGGGGCAAAAAGGAATTTGTTGAATTTGTATCAATGCAGTTCCTCTGTATTATATGTCAAGAGGTCGCAGCTAGTCCTGTAACGACACCTTGCCTACATAACTTCTGTATGGCGTGTTTAAAATTGGCCTTCAAAACAACCGGAGCGATTTGTCCGTGCTGCAGACAGAACTTAGCAAAATATGAACCGGAGCCTAACGAAGAGTTGAAAACCGCACTACGTAGCATCATGACCGGCTACGACGCTGGAAAGAAGTAA

Protein

MVKINTDSPKKSLTKSTADDKTDSDNNKNHEQESSDAVYTDAVSKLYAVGDLVDVRDPEHGAWFESKIIRIVHDQKGKKSEHEKEKGSDTSSSYQSQNYDCNDLENKPPIEMNGDCKPKSKGIAKYFTKSPKGKKKDKDSKSETRIVDNLLYKVQLDNDEDDSESYCKEKDIRPRARTIIDIKDLKVGQTVMVNYNIEEPMEKGYWFDFKVSEIRKLRTSYDLVGTLYLGPEAVPQNDTKIRLQDKIFAIEEVVPLEKRSDEYNRLMTMNPEKRSQPLTCLTCRDDEEALCKDCGCYVCLGKEFPEKIVLCDECNYGYHMSCLKPPLKDLPDEDWYCPSCKRDPQDVVAPGAAKQTKKKNASQSKRDWGRGMACVGKTKTCAMPANHFGPIPGIEVGMCWRFRIQLSESGVHRPPVSGIHGRDVEGAYSIVLSGGYEDDVDNGNEFTYTGSGGRDLSGNKRTAEQSCDQTLTRENKALARNCAVNQIKEDGADAGENWKNGKPVRVVRSYKMMKHFPKYAPSEGIRYDGIYKVVKYYPEKGLSGYRVWKYLLRRDDPSPAPWEAGAKHYPIVYPDGYLEAEAEKKALKQKQNTKSKGKVTKKRALRESNQSAESECDISPPAKKRKTASENKRKGKSSKTKSTTSPNGSILEMFSPKRRNSANGMKQVKKSESVLTSEERDAIKADTLNSKLWGECLAVCDSWGKKEFVEFVSMQFLCIICQEVAASPVTTPCLHNFCMACLKLAFKTTGAICPCCRQNLAKYEPEPNEELKTALRSIMTGYDAGKK

Description

Multidomain protein that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification. Specifically recognizes and binds hemimethylated DNA at replication forks via its YDG domain and recruits DNMT1 methyltransferase to ensure faithful propagation of the DNA methylation patterns through DNA replication. In addition to its role in maintenance of DNA methylation, also plays a key role in chromatin modification: through its tudor-like regions and PHD-type zinc fingers, specifically recognizes and binds histone H3 trimethylated at 'Lys-9' (H3K9me3) and unmethylated at 'Arg-2' (H3R2me0), respectively, and recruits chromatin proteins. Enriched in pericentric heterochromatin where it recruits different chromatin modifiers required for this chromatin replication. Also localizes to euchromatic regions where it negatively regulates transcription possibly by impacting DNA methylation and histone modifications. Has E3 ubiquitin-protein ligase activity by mediating the ubiquitination of target proteins such as histone H3 and PML. It is still unclear how E3 ubiquitin-protein ligase activity is related to its role in chromatin in vivo. May be involved in DNA repair.

Catalytic Activity

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.

Subunit

Interacts with DNMT3A and DNMT3B (By similarity). Interacts with DNMT1; the interaction is direct. Interacts with USP7; leading to its deubiquitination. Interacts with histone H3. Interacts with HDAC1, but not with HDAC2. Interacts with UHRF1BP1. Interacts with PML. Interacts with EHMT2. Binds hemimethylated CpG containing oligonucleotides.

Keywords

Cell cycle Chromatin regulator DNA-binding Metal-binding Nucleus Repeat Repressor Transcription Transcription regulation Transferase Ubl conjugation pathway Zinc Zinc-finger Complete proteome Reference proteome 3D-structure Acetylation Alternative splicing DNA damage DNA repair Isopeptide bond Phosphoprotein Polymorphism Ubl conjugation

Feature

chain  E3 ubiquitin-protein ligase UHRF1
splice variant  In isoform 2.
sequence variant  In dbSNP:rs17886098.

Uniprot

H9J310 A0A212F9M7 E2B263 A0A2S2QU71 A0A2R5L680 A0A2S2QEF3 + More
A0A147BFU7 A0A3L8DSG3 K7ISU7 A0A2S2P187 A0A0L7R8R5 A0A2S2PRQ6 A0A1L8HVT9 A0A151P0A1 A0A3M0JPL5 A0A2H8TSL8 A0A3Q0FXI9 J9JSH8 A0A093PNI2 A0A0P4VWV1 A0A1Y1MRM4 A0A2H8TED6 C3YSZ8 U3JEN0 A0A094KKY8 A0A091SIY6 B6CHA3 A0A099ZPB5 G7PYN1 F7DWN1 A0A2I3MBG9 A0A0A0MWK5 W5LVF4 A0A2K5WJF1 F6UA42 A0A2K5WJ60 A0A1D5Q791 M7B7H2 A0A131XNT4 A0A0B7A0A8 A0A091IG90 A0A2K5QI86 A0A2J8JR84 K7CBV7 A0A3B3Q5Y3 A0A087RBB0 A0A093R3P0 A0A2K6L5K6 A0A2K6RWL3 A0A2K6U314 A0A2Y9L5U9 H2QF26 A0A1S3FPB1 A0A226MA53 A0A2R9BE36 A0A3B3Q3W2 A0A1S3JWM3 F1PJN4 A0A2K6U2Z7 A0A3L7HL42 A0A091ML74 A0A218UCP9 A0A2K6U2Z8 A0A091IZR1 A0A2K6L5J9 A0A2K6RWL7 A0A2J8SC01 G3RVG5 A0A2Y9IHU4 Q96T88 A0A2J8SC09 A0A093GVQ4 A0A087WVR3 Q96T88-2 A0A091Q378 A0A093I1V0 A0A1A8F5I2 A0A091VN42 T2M6C9 F7ESL3 A0A1W4YE83 A0A2D0RT93 A0A087WTW0 W5U8Y3 S4RG48 A0A1W4Y5D1 H0WN70 A0A2U3VKN8 A0A2I0LQJ7 H2TMV9 A0A2J8SC22 A0A2U8UG34 R0L5N9 A0A087VQ90 A0A1A8V7R0 U3J4T9

EC Number

2.3.2.27

Pubmed

19121390 22118469 20798317 29652888 30249741 20075255 + More
27762356 22293439 28004739 18563158 22002653 17431167 20431018 23624526 29240929 25362486 16136131 24621616 22722832 16341006 29704459 22398555 10646863 17067204 17974005 14702039 15057824 15489334 12838312 15178447 15009091 15361834 17081983 16964243 17673620 18220336 17967883 18669648 19491893 19056828 19690332 19608861 20026581 20068231 21269460 21745816 21731699 21406692 23186163 22945642 22286757 22330138 22411829 25218447 28112733 18931436 18945682 18772889 21808300 21808299 21489993 21777816 22096602 22100450 22837395 11181995 24065732 23127152 23371554 21551351 23749191

EMBL

BABH01031259 AGBW02009564 OWR50446.1 GL445059 EFN60238.1 GGMS01012010 + More
MBY81213.1 GGLE01000824 MBY04950.1 GGMS01006921 MBY76124.1 GEGO01005751 JAR89653.1 QOIP01000004 RLU23385.1 AAZX01003324 GGMR01010493 MBY23112.1 KQ414631 KOC67234.1 GGMR01019521 MBY32140.1 CM004466 OCU00243.1 AKHW03001382 KYO42552.1 QRBI01000142 RMC01004.1 GFXV01004393 MBW16198.1 ABLF02025823 KL669216 KFW75815.1 GDRN01107613 GDRN01107612 GDRN01107610 JAI57396.1 GEZM01023617 JAV88271.1 GFXV01000615 MBW12420.1 GG666551 EEN56566.1 AGTO01021994 KL351516 KFZ59245.1 KK473652 KFQ58308.1 EU177101 KL895600 KGL82798.1 CM001294 EHH59097.1 JSUE03020408 JSUE03020409 JSUE03020410 JSUE03020411 AHZZ02013290 AHZZ02013291 AHZZ02013292 AHAT01011307 AQIA01033753 AQIA01033754 AQIA01033755 AQIA01033756 AQIA01033757 AQIA01033758 AAMC01100082 AAMC01100083 AAMC01100084 AAMC01100085 AAMC01100086 AAMC01100087 KB603100 EMP24217.1 GEFM01006943 JAP68853.1 HACG01027474 CEK74339.1 KL218529 KFP06360.1 NBAG03000432 PNI25278.1 PNI25280.1 PNI25282.1 PNI25284.1 GABC01005338 GABD01000894 GABE01004678 JAA06000.1 JAA32206.1 JAA40061.1 PNI25283.1 KL226268 KFM10764.1 KL445807 KFW95411.1 AACZ04041324 PNI25279.1 AWGT02013371 OXB52120.1 AJFE02066048 AJFE02066049 AJFE02066050 AJFE02066051 AJFE02066052 AJFE02066053 AJFE02066054 AJFE02066055 AJFE02066056 AJFE02066057 AAEX03012540 AAEX03012541 AAEX03012542 AAEX03012543 AAEX03012544 RAZU01000191 RLQ66573.1 KK829170 KFP75116.1 MUZQ01000430 OWK51489.1 KK501312 KFP14219.1 NDHI03003585 PNJ18307.1 PNJ18309.1 PNJ18311.1 PNJ18313.1 CABD030110990 CABD030110991 CABD030110992 CABD030110993 CABD030110994 CABD030110995 AF129507 AB177623 AB177624 AB075601 AF274048 EF560733 AK025578 AK289389 AK314579 AY787925 AC027319 AC053467 CH471139 BC113875 PNJ18312.1 KL205727 KFV73461.1 AC104525 KF459704 EAW69188.1 KK654751 KFQ04104.1 KK640007 KFV60653.1 HAEB01007556 SBQ54083.1 KL411250 KFR04832.1 HAAD01001611 CDG67843.1 AAMC01030976 AAMC01030977 AAMC01030978 AAMC01030979 AAMC01030980 AAMC01030981 JT408161 AHH38255.1 AAQR03059015 AAQR03059016 AAQR03059017 AAQR03059018 AAQR03059019 AKCR02000139 PKK19700.1 PNJ18308.1 MG734836 AWN02133.1 KB744239 EOA95597.1 KL501678 KFO14782.1 HADY01003405 HAEJ01015968 SBS56425.1 ADON01125454

Proteomes

UP000005204 UP000007151 UP000000311 UP000279307 UP000002358 UP000053825 + More
UP000186698 UP000050525 UP000269221 UP000189705 UP000007819 UP000053258 UP000001554 UP000016665 UP000053641 UP000009130 UP000006718 UP000028761 UP000018468 UP000233100 UP000008143 UP000031443 UP000054308 UP000233040 UP000261540 UP000053286 UP000233180 UP000233200 UP000233220 UP000248482 UP000002277 UP000081671 UP000198419 UP000240080 UP000085678 UP000002254 UP000273346 UP000197619 UP000053119 UP000001519 UP000248481 UP000005640 UP000053584 UP000053283 UP000192224 UP000221080 UP000245300 UP000005225 UP000245340 UP000053872 UP000005226 UP000016666

PRIDE

H9J310 Q96T88 A0A087WVR3

Pfam

PF00628   PHD        + More
PF00097   zf-C3HC4
PF02182   SAD_SRA
PF00240   ubiquitin
PF12148   TTD

Interpro

IPR000626   Ubiquitin_dom        + More
IPR021991   TTD_dom
IPR018957   Znf_C3HC4_RING-type
IPR019787   Znf_PHD-finger
IPR036987   SRA-YDG_sf
IPR001965   Znf_PHD
IPR013083   Znf_RING/FYVE/PHD
IPR017907   Znf_RING_CS
IPR015947   PUA-like_sf
IPR011011   Znf_FYVE_PHD
IPR003105   SRA_YDG
IPR029071   Ubiquitin-like_domsf
IPR001841   Znf_RING
IPR019956   Ubiquitin
IPR019786   Zinc_finger_PHD-type_CS

SUPFAM

SSF88697   SSF88697        + More
SSF54236   SSF54236
SSF57903   SSF57903

Gene 3D

2.30.280.10 3.30.40.10

ProteinModelPortal

H9J310 A0A212F9M7 E2B263 A0A2S2QU71 A0A2R5L680 A0A2S2QEF3 + More
A0A147BFU7 A0A3L8DSG3 K7ISU7 A0A2S2P187 A0A0L7R8R5 A0A2S2PRQ6 A0A1L8HVT9 A0A151P0A1 A0A3M0JPL5 A0A2H8TSL8 A0A3Q0FXI9 J9JSH8 A0A093PNI2 A0A0P4VWV1 A0A1Y1MRM4 A0A2H8TED6 C3YSZ8 U3JEN0 A0A094KKY8 A0A091SIY6 B6CHA3 A0A099ZPB5 G7PYN1 F7DWN1 A0A2I3MBG9 A0A0A0MWK5 W5LVF4 A0A2K5WJF1 F6UA42 A0A2K5WJ60 A0A1D5Q791 M7B7H2 A0A131XNT4 A0A0B7A0A8 A0A091IG90 A0A2K5QI86 A0A2J8JR84 K7CBV7 A0A3B3Q5Y3 A0A087RBB0 A0A093R3P0 A0A2K6L5K6 A0A2K6RWL3 A0A2K6U314 A0A2Y9L5U9 H2QF26 A0A1S3FPB1 A0A226MA53 A0A2R9BE36 A0A3B3Q3W2 A0A1S3JWM3 F1PJN4 A0A2K6U2Z7 A0A3L7HL42 A0A091ML74 A0A218UCP9 A0A2K6U2Z8 A0A091IZR1 A0A2K6L5J9 A0A2K6RWL7 A0A2J8SC01 G3RVG5 A0A2Y9IHU4 Q96T88 A0A2J8SC09 A0A093GVQ4 A0A087WVR3 Q96T88-2 A0A091Q378 A0A093I1V0 A0A1A8F5I2 A0A091VN42 T2M6C9 F7ESL3 A0A1W4YE83 A0A2D0RT93 A0A087WTW0 W5U8Y3 S4RG48 A0A1W4Y5D1 H0WN70 A0A2U3VKN8 A0A2I0LQJ7 H2TMV9 A0A2J8SC22 A0A2U8UG34 R0L5N9 A0A087VQ90 A0A1A8V7R0 U3J4T9

PDB

2ZKG E-value=9.75516e-76, Score=724

KEGG

101744602 K10638 E3 ubiquitin-protein ligase UHRF1 [EC:2.3.2.27] | (RefSeq) E3 ubiquitin-protein ligase UHRF1-

GO

GO:0005634 GO:0046872 GO:0016874 GO:0016567 GO:0010216 GO:0005720 GO:0061630 GO:0031493 GO:0044729 GO:0042802 GO:0005657 GO:0035064 GO:0051865 GO:0000122 GO:0010390 GO:0016363 GO:0016574 GO:0000792 GO:0007049 GO:0008270 GO:0006511 GO:0000790 GO:0004842 GO:0042393 GO:0000791 GO:0002088 GO:0006954 GO:0031100 GO:0044027 GO:0060729 GO:0001889 GO:0000987 GO:0032270 GO:0045944 GO:0008327 GO:0006281 GO:0050678 GO:2000373 GO:0005515 GO:0008076 GO:0005216 GO:0006811 GO:0016020 GO:0030246 GO:0007169 GO:0016021

Subcellular location

Nucleus

Length:

787

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.02602

Exp number, first 60 AAs:

0

Total prob of N-in:

0.00021

outside

1 - 787

Pi

21.867886

Theta

27.180689

Tajima's D

-0.923175

CLR

0.045736

CSRT

0.152792360381981

Interpretation

Uncertain

Sequence

Summary

Ontologies

Topology

Population Genetic Test Statistics