Gene

KWMTBOMO00462  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA013328

Annotation

tudor_staphylococcus/micrococcal_nuclease_[Bombyx_mori]

Location

Bomo_Chr1(-):13453299-13462720      View in EpiBrowser

Full name

Staphylococcal nuclease domain-containing protein       + More
Staphylococcal nuclease domain-containing protein 1      

Alternative Name

Tudor staphylococcal nuclease

Location in the cell

Cytoplasmic   Reliability : 1.87 Mitochondrial   Reliability : 1.382

CDS

ATGAGTGCTCCAGCACCGGCACCTGCTTACAAAATAGGAATAGTGAAGCAGGTACTATCGGGAGATACGGTGGTAATAAGAAAACAGCCTCAGGGCGGGCCGCCTCCAGAGAAAGTGATAGCCCTTTCGGGTATCACCGCACCTAAACTCGCTCGCCAAAGGACCGCCAACAATGACACAGAAACGAAGGATGAACCATTCGCGTGGGAAGCCCGTGAATTCCTAAGGAAGAAGCTAGTCGGCAAGGAGGTTATCTTCACTGCTGAGAAACCGGCGAATTCAGCGAATAGAGAATATGGCGTCGTATGGGCTGGCAAAAATCCTTCCAAAGATGAAAATGTTACTGAAGCCTTGCTGGCTGAAGGTCTCGTTAAAGTTCGAGAAGGTGCTAGAAATATACCTCAGCTCAAAAAGCTGGTAGAGATCGAAGAGACAGCCAAATCGCAAGGCAAAGGAATTTGGAGTACTGATGCTGATAAACATGTACGAGACATCAAGTGGTCTATCGAAAATCTGAAAGCTTTCGTCAACAAATACAATGGTAAGCCTGTAAAGGCTATCATTGAATATGTGCGTGATGGTTCTACAGTACGTCTTTGTTTGTTACCTGAATACACTCCCATTACACTGATGCTATCTGGAATTCGATGTCCCGCTGTAAGACAAGACGGTGAATCTGAGCCTTACGCAGAAGAAGCACGCTTCTTCCTGGAGTCCAGATTGCTGCAGAAAGACGTCGAAGTAGTTCTGGAGTCCGTTAATAACAACAATATCGTGGGCACGATACTGCACCCGCAGGGTAACATCGCTGAGGCCCTACTAAGACAAGGGTTCGCGAAATGTGTCATGAAATCAGGTGCATCTACATTGAAAGCCGCCGAAAGTGCAGCTAAGGAAGCAAAACTGCGCATATGGATAAATTATGTAAGCAATGCACCTATAATCCCGGCGAAAGACAAGGAATTCACGGCCACCGTGTTGGAAGTAGTCAACGGAGACGCGCTCGTTGTTAAAACACATAGCAATGTACAGAAAAAAATATTCTTAGCTAGCGTCAGGCCTCCTCGTGAAAAGAGCAATGCTGACGACGACAATAAACCTATACCGAGGCCAAAAGGATTTAGACCTTTGTATGATATTCCATGGATGTACGAGGCACGTGAATTTCTTAGAAAAAAACTGGTTGGGAAAAAAGTTAATGTGACAGTAGATTACATTCAGCCAGCAAAAGATAATTTCCCAGAAAAGACATGTTGTACTGTCGTAAGCGGTGGCACGAACATAGCTGAAGCTCTGGTGACTAAAGGGTTGGCAACAGTGAAGTACAGAAACGATAATGACCAAAGAAGCTCACAGTATGATAAATTATTGGAGGCTGAATTGAAAGCAGTTAAGGCCGCAGTTGGTATTCATAACAAGAAAGAAGTGCCCACCCACCGTGTGCAAGACACCAGTGGAGATCCAACGAAAGCAAAAAAATTCTTCCCATTCCTCAAACGTGCCCAAAAAACCGAGGCTACTGTGGAATTCGTTGCTAGCGGATCGCGTATGCGTTTATATATTCCTAAAGAATCCGTCTTAGTAACCTTTTTGCTTGCCGGCATTAACTGTCCCCGAGGTGCGAGACCTGCAATTGGCGGAGGACCCAAACAAAATGCAGAGCCTTTCGGAGAAGAAGCTTTGCAGTTCACGAAAGAAAAGTGCTTACAGCACGACGTCTTAGTCAGCATAGAAGAGATTGACAAAGCAGGAAACTTCATCGGTTGGCTCTGGGTTGACAACGAGAATTTGTCCGTTTCGTTAGTCGAACATGGCCTTGCCTCGGCGCATCACACGGCGGAAACGTCTGAGTTTGCACGCGCCATAAAAACCGCTGAAGAGAATGCAATTAAAAAGCGTATCGGCGTATGGAAAGACTATGTCGAAGAAGAAAAAGAAGTCGAAAAGGAGAGAAATGCAACCGTACAAGATCGTACTCTTAAATACGACCGCGTGGTCATCACTGAAGTCACTCCTGAAGGTCATTTCTACGCACAAAATGTCGATTTAGGTGTGAAACTAGAAAGTCTTATGGAAACTATACACACTGAGTTTAGAAACAGTCACCCATTGCCTGGATCTTACGCTCCAAGGAAGGGAGCCATCTGTGCTGCTCGTTTTACAGCCGATGACCAATGGTACAGGGCAAAGATTGAAAAAATAACAGACAATAGGCAGGTCCAAGTTGTATACATCGACTATGGAAATCGAGAGACGTTAGACATAACCAGATTGGCAGCACTCCCGGTGGGTACTGAGCACGATTCGCCTTTTGCGACAGAGTATGTTCTCTGCTGTGTGAAATTCCCCTCGGACCCTGATGACCGAGCAGAAGCTGTAACCAGTTTCTATAATGATGTCGTTGGCAAGAGGCTGCTCTTGAATGTAGAAATCCGTGGATCACCAGCTGCTGTTACCCTTGTTGATCCTAACACAAACATTGATCTGGGCAAGAACCTTATCAAAGATGGGCTCGTGTTAGTGGAGCAGGTTCGTGACAGCCGACTGGCAACATTGATGGCCGAGTATCGCGCCGCTCAGGAGCACGCCAAGAGCTCGCGACTCAACTTGTGGCGGCACGGTGACATCACCGAGGACGATGCGGTAGAATTCGGGGTGCGTCGCTAG

Protein

MSAPAPAPAYKIGIVKQVLSGDTVVIRKQPQGGPPPEKVIALSGITAPKLARQRTANNDTETKDEPFAWEAREFLRKKLVGKEVIFTAEKPANSANREYGVVWAGKNPSKDENVTEALLAEGLVKVREGARNIPQLKKLVEIEETAKSQGKGIWSTDADKHVRDIKWSIENLKAFVNKYNGKPVKAIIEYVRDGSTVRLCLLPEYTPITLMLSGIRCPAVRQDGESEPYAEEARFFLESRLLQKDVEVVLESVNNNNIVGTILHPQGNIAEALLRQGFAKCVMKSGASTLKAAESAAKEAKLRIWINYVSNAPIIPAKDKEFTATVLEVVNGDALVVKTHSNVQKKIFLASVRPPREKSNADDDNKPIPRPKGFRPLYDIPWMYEAREFLRKKLVGKKVNVTVDYIQPAKDNFPEKTCCTVVSGGTNIAEALVTKGLATVKYRNDNDQRSSQYDKLLEAELKAVKAAVGIHNKKEVPTHRVQDTSGDPTKAKKFFPFLKRAQKTEATVEFVASGSRMRLYIPKESVLVTFLLAGINCPRGARPAIGGGPKQNAEPFGEEALQFTKEKCLQHDVLVSIEEIDKAGNFIGWLWVDNENLSVSLVEHGLASAHHTAETSEFARAIKTAEENAIKKRIGVWKDYVEEEKEVEKERNATVQDRTLKYDRVVITEVTPEGHFYAQNVDLGVKLESLMETIHTEFRNSHPLPGSYAPRKGAICAARFTADDQWYRAKIEKITDNRQVQVVYIDYGNRETLDITRLAALPVGTEHDSPFATEYVLCCVKFPSDPDDRAEAVTSFYNDVVGKRLLLNVEIRGSPAAVTLVDPNTNIDLGKNLIKDGLVLVEQVRDSRLATLMAEYRAAQEHAKSSRLNLWRHGDITEDDAVEFGVRR

Description

Endonuclease that mediates miRNA decay of both protein-free and AGO2-loaded miRNAs.
Endonuclease which shows activity towards both DNA and RNA substrates (PubMed:14508492, PubMed:26808625). Has a role in translation regulation throught its association with the with the RNA-induced silencing complex (RISC) (PubMed:14508492, PubMed:26808625). Plays a role in spermatogenesis probably by negatively regulating piwi expression in the germline (PubMed:26808625). Together with piwi, might be involved in transposon repression in the germline (PubMed:26808625).

Catalytic Activity

Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products.

Subunit

Associates with the RNA-induced silencing complex (RISC) (PubMed:14508492). Interacts with the RISC components AGO2, Fmr1 and vig (PubMed:14508492). Interacts with piwi (PubMed:26808625).

Keywords

3D-structure   Complete proteome   Cytoplasm   Endonuclease   Hydrolase   Nuclease   Nucleus   Reference proteome   Repeat   RNA-binding   RNA-mediated gene silencing  

Feature

chain  Staphylococcal nuclease domain-containing protein

Uniprot

D9N4J4   H9JUW5   A0A2W1BLH6   A0A212EQK0   S4PY67   A0A2H1W6B7   + More
A0A2A4J4G7   A0A194QN47   A0A194PF40   A0A2J7RK75   A0A2J7RK72   A0A067RA05   A0A1L7NZM8   A0A1Y1NAS3   A0A0P5RPB4   A0A0P6ECC7   A0A0P6D5Y7   A0A0P5JM29   D6WH29   A0A0P5M5M6   A0A0P6DAC5   A0A0T6AYP2   A0A0P5NMF8   A0A0P5MWB4   A0A0P6DA35   A0A0P6F8J1   A0A0P6IWG6   A0A0P5VNB2   A0A0P5BRP3   A0A0P5UMH7   A0A0P5IFM4   A0A0P5BRR9   A0A0P5Y6Z4   A0A0N8C6G6   A0A0N8B3V9   Q17PM3   A0A0P4Z1P6   A0A023EY38   A0A126Q9D5   A0A182H564   E9GT82   A0A1W4XCF9   K1R512   A0A0N8DHY8   A0A1B6EL67   A0A0P5XQE1   U5EY13   A0A0P5HPX3   A0A1S3IS83   A0A0P5G4Z2   V3ZN51   A0A0P5KVE5   A0A0N8D1M8   A0A0P5SXH0   A0A1B6ECM6   B0WIK3   A0A1Q3F1Q3   A0A0P5RCA0   A0A1Q3F1R2   A0A210Q8D8   A0A0P5IES6   A0A0P5RAE9   A0A2C9JNE9   A0A1Z5LGF5   A0A0P4ZBL9   A0A0P5AQQ8   A0A2R5LE81   A0A0P6G5Q7   A0A0P6EE22   A0A1L8DGR0   G1ARD5   A0A0P5K1F1   A0A0P5VBJ5   A0A0P5VEH1   A0A0B6ZVN1   A0A0N8CGJ0   A0A1J1IXN9   A0A2I9LPS2   A0A087ULG5   A0A0P5QNC1   A0A0P4XWZ0   A0A0P5IIZ5   A7RXU0   A0A1I8N1Y7   N1PBE4   A0A0P5R6M2   A0A0P4ZZC7   A0A0A1WIC5   A0A1W4VNR6   T1PB73   A0A0P4Z5X6   B4KXY0   B4MGQ8   B4QEY1   A0A0L8GAX7   A0A0M3QVT8   N6TSV1   Q9W0S7   A0A1I8PTZ6   B3NEM9  

EC Number

3.1.31.1

Pubmed

19121390    28756777    22118469    23622113    26354079    24845553    + More
28034629    28004739    18362917    19820115    17510324    24945155    26483478    21292972    22992520    23254933    28812685    15562597    28528879    21745576    29248469    17615350    25315136    25830018    17994087    18057021    22936249    23537049    10731132    12537572    12537569    14508492    26808625    19232356   

EMBL

AB573814    BAJ14101.1    BABH01039784    KZ149977    PZC75899.1    AGBW02013269    + More
OWR43749.1    GAIX01003818    JAA88742.1    ODYU01006633    SOQ48645.1    NWSH01003445    PCG66282.1    KQ461196    KPJ06400.1    KQ459605    KPI91897.1    NEVH01002981    PNF41237.1    PNF41236.1    KK852598    KDR20540.1    LC194150    BAW35372.1    GEZM01007667    JAV95011.1    GDIQ01097934    GDIQ01097933    JAL53793.1    GDIQ01189943    GDIQ01189942    GDIQ01065491    JAN29246.1    GDIQ01082634    GDIQ01082633    JAN12104.1    GDIQ01202950    JAK48775.1    KQ971321    EFA00120.1    GDIQ01160459    GDIQ01160458    JAK91267.1    GDIQ01216742    GDIQ01081079    JAN13658.1    LJIG01022488    KRT80307.1    GDIQ01140261    JAL11465.1    GDIQ01158668    JAK93057.1    GDIQ01081080    JAN13657.1    GDIQ01064049    GDIQ01064048    JAN30688.1    GDIQ01003523    JAN91214.1    GDIP01146872    GDIP01146871    GDIP01112182    GDIP01043182    LRGB01002451    JAL91532.1    KZS07676.1    GDIP01181026    JAJ42376.1    GDIP01110926    JAL92788.1    GDIQ01217840    GDIQ01025906    JAK33885.1    GDIP01185375    GDIQ01111869    JAJ38027.1    JAL39857.1    GDIP01200461    GDIP01062040    JAM41675.1    GDIQ01110131    JAL41595.1    GDIQ01215387    JAK36338.1    CH477190    EAT48729.1    GDIP01219015    JAJ04387.1    GAPW01000149    JAC13449.1    KT694370    AMK01822.1    JXUM01110873    JXUM01110874    JXUM01110875    KQ565451    KXJ70956.1    GL732563    EFX77404.1    JH818113    EKC40848.1    GDIP01031943    JAM71772.1    GECZ01031128    JAS38641.1    GDIP01068776    JAM34939.1    GANO01000655    JAB59216.1    GDIQ01226537    JAK25188.1    GDIQ01247185    JAK04540.1    KB203251    ESO85752.1    GDIQ01180052    JAK71673.1    GDIP01077623    JAM26092.1    GDIP01133928    JAL69786.1    GEDC01001617    JAS35681.1    DS231949    EDS28526.1    GFDL01013559    JAV21486.1    GDIQ01111871    JAL39855.1    GFDL01013550    JAV21495.1    NEDP02004628    OWF45007.1    GDIQ01215389    JAK36336.1    GDIQ01110130    JAL41596.1    GFJQ02000580    JAW06390.1    GDIP01219067    JAJ04335.1    GDIP01209815    JAJ13587.1    GGLE01003676    MBY07802.1    GDIQ01048909    JAN45828.1    GDIQ01064051    JAN30686.1    GFDF01008442    JAV05642.1    JF429696    AEK49107.1    GDIQ01196186    JAK55539.1    GDIP01117522    JAL86192.1    GDIP01116233    JAL87481.1    HACG01025526    HACG01025527    CEK72391.1    CEK72392.1    GDIP01133927    JAL69787.1    CVRI01000059    CRL03321.1    GFWZ01000395    MBW20385.1    KK120390    KFM78204.1    GDIQ01111872    JAL39854.1    GDIP01237824    JAI85577.1    GDIQ01215390    JAK36335.1    DS469551    EDO43681.1    AMQN01000055    KB291798    ELU18909.1    GDIQ01110128    JAL41598.1    GDIP01219068    JAJ04334.1    GBXI01015513    JAC98778.1    KA645996    AFP60625.1    GDIP01219066    JAJ04336.1    CH933809    EDW17652.1    CH940682    EDW57124.1    CM000362    CM002912    EDX07008.1    KMY96614.1    KQ422894    KOF74019.1    CP012525    ALC42969.1    APGK01055648    KB741269    ENN71421.1    AE014296    AY069361    BT003270    AAL39506.1    AAO25027.1    CH954178    EDV50024.1   

Proteomes

UP000005204    UP000007151    UP000218220    UP000053240    UP000053268    UP000235965    + More
UP000027135    UP000007266    UP000076858    UP000008820    UP000069940    UP000249989    UP000000305    UP000192223    UP000005408    UP000085678    UP000030746    UP000002320    UP000242188    UP000076420    UP000183832    UP000054359    UP000001593    UP000095301    UP000014760    UP000192221    UP000009192    UP000008792    UP000000304    UP000053454    UP000092553    UP000019118    UP000000803    UP000095300    UP000008711   

PRIDE

D9N4J4    H9JUW5    Q9W0S7   

Pfam

PF00567   TUDOR        + More
PF00565   SNase
PF00071   Ras

Interpro

IPR016071   Staphylococal_nuclease_OB-fold        + More
IPR035437   SNase_OB-fold_sf       
IPR016685   Silence_cplx_Nase-comp_TudorSN       
IPR002999   Tudor       
IPR020849   Small_GTPase_Ras-type       
IPR027417   P-loop_NTPase       
IPR001806   Small_GTPase       
IPR005225   Small_GTP-bd_dom       

SUPFAM

SSF52540   SSF52540       

Gene 3D

2.40.50.90    

ProteinModelPortal

D9N4J4   H9JUW5   A0A2W1BLH6   A0A212EQK0   S4PY67   A0A2H1W6B7   + More
A0A2A4J4G7   A0A194QN47   A0A194PF40   A0A2J7RK75   A0A2J7RK72   A0A067RA05   A0A1L7NZM8   A0A1Y1NAS3   A0A0P5RPB4   A0A0P6ECC7   A0A0P6D5Y7   A0A0P5JM29   D6WH29   A0A0P5M5M6   A0A0P6DAC5   A0A0T6AYP2   A0A0P5NMF8   A0A0P5MWB4   A0A0P6DA35   A0A0P6F8J1   A0A0P6IWG6   A0A0P5VNB2   A0A0P5BRP3   A0A0P5UMH7   A0A0P5IFM4   A0A0P5BRR9   A0A0P5Y6Z4   A0A0N8C6G6   A0A0N8B3V9   Q17PM3   A0A0P4Z1P6   A0A023EY38   A0A126Q9D5   A0A182H564   E9GT82   A0A1W4XCF9   K1R512   A0A0N8DHY8   A0A1B6EL67   A0A0P5XQE1   U5EY13   A0A0P5HPX3   A0A1S3IS83   A0A0P5G4Z2   V3ZN51   A0A0P5KVE5   A0A0N8D1M8   A0A0P5SXH0   A0A1B6ECM6   B0WIK3   A0A1Q3F1Q3   A0A0P5RCA0   A0A1Q3F1R2   A0A210Q8D8   A0A0P5IES6   A0A0P5RAE9   A0A2C9JNE9   A0A1Z5LGF5   A0A0P4ZBL9   A0A0P5AQQ8   A0A2R5LE81   A0A0P6G5Q7   A0A0P6EE22   A0A1L8DGR0   G1ARD5   A0A0P5K1F1   A0A0P5VBJ5   A0A0P5VEH1   A0A0B6ZVN1   A0A0N8CGJ0   A0A1J1IXN9   A0A2I9LPS2   A0A087ULG5   A0A0P5QNC1   A0A0P4XWZ0   A0A0P5IIZ5   A7RXU0   A0A1I8N1Y7   N1PBE4   A0A0P5R6M2   A0A0P4ZZC7   A0A0A1WIC5   A0A1W4VNR6   T1PB73   A0A0P4Z5X6   B4KXY0   B4MGQ8   B4QEY1   A0A0L8GAX7   A0A0M3QVT8   N6TSV1   Q9W0S7   A0A1I8PTZ6   B3NEM9  

PDB

3BDL     E-value=1.91182e-136,     Score=1248

KEGG

100499531    K15979 staphylococcal nuclease domain-containing protein 1 | (RefSeq) Tudor-SN; tudor staphylococcus

GO

GO:0005737   GO:0004521   GO:0016442   GO:0031047   GO:0046872   GO:0004518   GO:0003723   GO:0005634   GO:0006401   GO:0005525   GO:0016020   GO:0007165   GO:0003924   GO:0007283   GO:0010529   GO:0005829   GO:0004519   GO:0043565   GO:0003676   GO:0030126   GO:0005488   GO:0003677   GO:0003707   GO:0007169   GO:0016021  

Subcellular location

Cytoplasm  
Nucleus   Associates with ribosomes.   With evidence from 4 publications.

Length:

888

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.00121

Exp number, first 60 AAs:

0.00077

Total prob of N-in:

0.00006

outside

1  -  888

 

 

Pi

19.656589

Theta

16.2085

Tajima's D

0.40673

CLR

0.129873

CSRT

0.496075196240188

Interpretation

Uncertain