Gene

KWMTBOMO00388

Pre Gene Modal

BGIBMGA000719

Annotation

PREDICTED:_membrane-bound_transcription_factor_site-1_protease_[Amyelois_transitella]

Location

Bomo_Chr1(+):11423959-11450837      View in EpiBrowser

Full name

Membrane-bound transcription factor site-1 protease      

Alternative Name

Endopeptidase S1P
Subtilisin/kexin-isozyme 1

Location in the cell

PlasmaMembrane   Reliability : 1.048

CDS

ATGGGGTTCACCCATACAATTCTTCTAAGTGTAATGCTTTATCTCCCTATCTTATTAATTTCTTCTAAAGAAGTGCGTGAAAAAAATGAAAATTGCAATACCACTGAGTCTGAACGTGTTCAATACGAGTTCACTACCGATGTTGTTAAAAGTCAGCACATAATAACATTTAAAGGATACTATCCCAGGAGCACAAGAGAAAATTATGTAAATGCCGCTCTAACCAGTGCAGGTATAACAGACTGGAAGATTATTAAACGCAACAATCCAGCCAAAGAATATCCTAGTGACTTTGATGTTGTTGTCCTAGAAAATGCAGGAAGAAAAGCATTGGAAGCCCTGCAAGATCACCCAGCAGTTCGACGGGTCACAGCCCAACGTCAAGTCACACGGTCCATAAAGTATGTTAAAGAGGATGAGTGTGGATCAGGGGGCTGTTTGTATTCTGGGTGGCGGAATTATCGCGGAAGAGGACTGCACTCTCTTCGAAAAACTCGTGACAATGGATACACGTCACGCAAACTATTACGGACTGTCCCGCGCCAGATCACCTCTGTGTTGAAAGCAGACGTTCTTTGGTCTCTTGGTGTCACAGGTGAAGGTATAAAGGTCGCCGTGTTTGATACCGGCTTAGCTCGTAACCATCCTCACTTTGGGCGGATCCGTGAACGTACCGACTGGACGGGAGAAGACACATTGGACGACGCCTTAGGCCACGGAACGTTCGTTGCCGGAGTAATAGCTTCGAAGGCCGACTGTTTGGGATTCGCTCCCGACGCCGATTTGCACATATTCCGAGTATTCACTGATAATCAGGTTTCATACACTTCGTGGTTCCTGGACGCGTTCAACTACGCAATAATGCGCAAAATAGACGTTCTCAATCTCAGCATCGGGGGACCGGATTTTATGGATCATCCGTTTGTCGACAAAGTCTGGGAACTCAGTGCTAATAAGGTAATTATGGTATCGGCGATCGGTAACGACGGTCCTCTGTACGGCACACTGAACAACCCCGCTGATCAGATGGACGTCATAGGTGTCGGCGGTATAGGGTTCGATGACCGCATCGCGAAATTTTCGTCGAGAGGAATGACAACATGGGAGTTACCACAAGGTTACGGTCGTATGAAGCCCGACATTGTAACGTACGGCAGTGGAGTCCGTGGATCGAGCGTGAATGGTGGATGTAAATCACTTAGCGGTACATCGGTAGCGTCGCCGGTTGTAGCTGGCGCCATAGCGTTACTAGCGAGCGGTGTTCCACGTCAGAATTTAACTCCGGCATCTGTTAAACAAGCCTTATGCAGCACAGCGCTAAGACTACAAGGGCCTAACATGTTCGAACAGGGACACGGGAAATTGGACCTTATCAGCGCTTATCAGTTCCTCAGGAAGTACGAGCCGCAAGCTACATTAAGCCCGAATTACATTGACCTCACTGAATGTCAGTACATGTGGCCGTACTGCACTCAGCCGCTGTATTACAGCTCGCAGCCAACGATCGCGAACGTGACCGTCATTAATGGACTGAGCGTCGTTGGGAATGTGAAAGAAGTCTCGTGGCATCCACATCTGCCCCATGGCGTTCTACTCTCGGTGTCTTCAGATTACAGCGATGTCCTCTGGCCTTGGTCTGGCTGGCTGGCCCTCAGCTTCACAGTCCGGGAAGAAGGGGCTAACTTTGATGGTATCATCGAGGGTCACGTGAACGTAACGATTGAAAGTCACGACGATACAGGTGATAGAACAATAAAGAACGCCACACTCACATTGCCGATACGAGCTCGAGTGATCCCAGTTCCGGTGCGATCCCGGCGTCTCCTCTGGGACCAGTTCCACAGTCTACGTTACCCCGGCGGCTACTTCCCTAGGGACGATCTACGAGCCAAACACGATCCCCTCGACTGGCACGCCGATCATATACACACCAATTTTAGGGACATGTACAGGAGATTGCGCGAACACGGATTCTACGTTGAAGTCATGGGTACACCCTTAACATGTATCGACACGTCGCTCTACGGCGCCCTGTTACTCGTTGACCCCGAAGATGAATATTTCCCCGAAGAGATGGCAACATTGAAGAAAGCCGTTGACGCTGGACTATCGTTAATTGTTTTCGCCGATTGGTACAACGCTTCCCTTCTGCGGCACGTGAAGTTTTACGACGAAAACACAAGGCAATGGTGGATTCCTGAGACGGGAGGATCAAACGTTCCCGCTCTGAACGATTTACTCAGCATGTATCAGGTGGCGCTAGGCGATAGAGTATTTGAAGGTTCATTCAAACTGGGCGGACACCCGATGTATTACGCGAGCGGAACTCACATACACAGCTTCCCTGAACACGGTGTACTGATCACGGCCCCTCTCAACGATCAAGGGCAACAGATAATATCAGGTGAAAAGCCGGGGGCCGCGAACGGCAAAACGGAAGTCACACAACCGTTGCAAGTCCCGATCCTAGGTCTCCTACAAACTGATGGAGATATTCATGACTATTCAAACGATACCGTGGATAAAGTACCTAAGTCTGGCCGTCTGGTCGTTTACGGAGATTCATCTTGTCTGGAAGGTGGAACTGCCCGGAACTGTCACTGGTTGCTTTTGGCCGCTTTACAATACGCAATAGTGGGTCACGTGCCTCCAGCTCTCAAAGAAGCCGCAACGCCGCAAGAGAAACGCGTCAGAGATGTGCATATAATCCCTTCAGAACTGCCTCGACGTGCCGAAGGGGGACGTCTCCATGTCTACTCTCGCGTCCTATCGCCCGATGGAAGTGGACCACGTCCAATACCAGAATGCTTCAGTCCCACCCCGCTACCGTCCCAGCCAGTACACGCTGCTCCATCTGCCAGGACATTGGCTCCTAGACATCAGCCTACAGATCCTAAGAGTATCGGTCCTCCAGAAATAGAAGGCACCGAACCAGCGCCCAGAGCGTGGCGAGGAGCAGGCGCCGCGCCTTCCAGAGTTCACCCGCAGTCGGAAACGACACCCACTAGTCTAACGAACCGAGCTATCACACTACTAGCGATCGTAACTGCTATATACACTGTCGCTCAGCTGTGGAAGCGGTGGGGCCGCAGGATCCGGAGACGCAGAATCATCTCTCTAGCCACCTAG

Protein

MGFTHTILLSVMLYLPILLISSKEVREKNENCNTTESERVQYEFTTDVVKSQHIITFKGYYPRSTRENYVNAALTSAGITDWKIIKRNNPAKEYPSDFDVVVLENAGRKALEALQDHPAVRRVTAQRQVTRSIKYVKEDECGSGGCLYSGWRNYRGRGLHSLRKTRDNGYTSRKLLRTVPRQITSVLKADVLWSLGVTGEGIKVAVFDTGLARNHPHFGRIRERTDWTGEDTLDDALGHGTFVAGVIASKADCLGFAPDADLHIFRVFTDNQVSYTSWFLDAFNYAIMRKIDVLNLSIGGPDFMDHPFVDKVWELSANKVIMVSAIGNDGPLYGTLNNPADQMDVIGVGGIGFDDRIAKFSSRGMTTWELPQGYGRMKPDIVTYGSGVRGSSVNGGCKSLSGTSVASPVVAGAIALLASGVPRQNLTPASVKQALCSTALRLQGPNMFEQGHGKLDLISAYQFLRKYEPQATLSPNYIDLTECQYMWPYCTQPLYYSSQPTIANVTVINGLSVVGNVKEVSWHPHLPHGVLLSVSSDYSDVLWPWSGWLALSFTVREEGANFDGIIEGHVNVTIESHDDTGDRTIKNATLTLPIRARVIPVPVRSRRLLWDQFHSLRYPGGYFPRDDLRAKHDPLDWHADHIHTNFRDMYRRLREHGFYVEVMGTPLTCIDTSLYGALLLVDPEDEYFPEEMATLKKAVDAGLSLIVFADWYNASLLRHVKFYDENTRQWWIPETGGSNVPALNDLLSMYQVALGDRVFEGSFKLGGHPMYYASGTHIHSFPEHGVLITAPLNDQGQQIISGEKPGAANGKTEVTQPLQVPILGLLQTDGDIHDYSNDTVDKVPKSGRLVVYGDSSCLEGGTARNCHWLLLAALQYAIVGHVPPALKEAATPQEKRVRDVHIIPSELPRRAEGGRLHVYSRVLSPDGSGPRPIPECFSPTPLPSQPVHAAPSARTLAPRHQPTDPKSIGPPEIEGTEPAPRAWRGAGAAPSRVHPQSETTPTSLTNRAITLLAIVTAIYTVAQLWKRWGRRIRRRRIISLAT

Description

Serine protease that catalyzes the first step in the proteolytic activation of the sterol regulatory element-binding proteins (SREBPs). Other known substrates are BDNF, GNPTAB and ATF6. Cleaves after hydrophobic or small residues, provided that Arg or Lys is in position P4. Cleaves known substrates after Arg-Ser-Val-Leu (SERBP-2), Arg-His-Leu-Leu (ATF6), Arg-Gly-Leu-Thr (BDNF) and its own propeptide after Arg-Arg-Leu-Leu. Mediates the protein cleavage of GNPTAB into subunit alpha and beta, thereby participating in biogenesis of lysosomes.

Catalytic Activity

Processes precursors containing basic and hydrophobic/aliphatic residues at P4 and P2, respectively, with a relatively relaxed acceptance of amino acids at P1 and P3.

Cofactor

Ca(2+)

Similarity

Belongs to the peptidase S8 family.

Keywords

Autocatalytic cleavage   Calcium   Cholesterol metabolism   Complete proteome   Direct protein sequencing   Endoplasmic reticulum   Glycoprotein   Golgi apparatus   Hydrolase   Lipid metabolism   Membrane   Phosphoprotein   Polymorphism   Protease   Reference proteome   Serine protease   Signal   Steroid metabolism   Sterol metabolism   Transmembrane   Transmembrane helix   Zymogen  

Feature

chain  Membrane-bound transcription factor site-1 protease
sequence variant  In dbSNP:rs34701895.

Uniprot

A0A2A4JC15   A0A212EGG8   A0A194PGX1   A0A194QLD6   A0A1Y1LMH9   A0A1Y1LPH7   + More
D6WIB2   A0A139WLB7   A0A1W4WJX8   A0A1W4WIY9   A0A2J7PZP8   A0A2J7PZR5   A0A067QSV2   A0A0T6B9G1   A0A2A3EAP1   T1ISC2   A0A088A5Z3   A0A232EJF7   T1J4A8   A0A1L8GKG1   Q6AX98   A0A1L8GKI6   A0A1S3HYQ9   A0A154P3R4   Q0VA23   M3YC21   A0A2Y9K244   A0A384BZG3   A0A091S4F9   A0A0C9QMP8   F6S2F2   D2HRI4   A0A2Y9IG02   V9K9C3   A0A2Y9SY48   A0A384A5I7   G1KN46   A0A341AP56   Q08E55   A0A2Y9P8W9   A0A3L8SFL4   A0A2Y9SPV3   A0A2Y9NXL1   A0A099Z1P2   A0A093PUW3   A0A2Y9DNI6   A0A340X0E6   A0A2Y9K2N3   Q14703   F1PER6   A0A0P6JL18   S7MMG9   R0L9K5   A0A099ZYJ0   M3WFB1   G9K9U7   A0A151MLJ3   I3MBB7   G1RJD6   A0A286ZZH4   A0A2K5KZ34   A0A2U4BRP0   A0A1U7RFK3   A0A1U7R8F2   H0V6Z3   A0A2K6CMT2   A0A2K5V1G2   A0A2K5V1H8   A0A0D9S1D8   A0A2U4BRQ6   A0A096NPH3   H9ZES7   A0A2K5Z4Q0   H9G192   F7G9K2   A0A131YEJ8   A0A091GGY9   H0Z8S1   H2QBL9   E0VQL9   A0A2I0UCW7   A0A091N8D2   Q1LWH3   A0A1I9XGB5   A0A2K6K2W1   A0A2K6R346   A0A2K5IJ30   A0A131YQA9   A0A091VER6   A0A094KTH6   A0A1I9XGB3   G3SXF6   A0A091WI36   A0A1V4K102   A0A091N6X0   A0A091TEW2   L8I827   Q5R4I7   A0A2K5F5D8  

EC Number

3.4.21.112

Pubmed

22118469    26354079    28004739    18362917    19820115    24845553    + More
28648823    27762356    24813606    20431018    20010809    24402279    19393038    30282656    10944850    7788527    14702039    15616553    15489334    17974005    10644685    9990022    21719679    26091039    16341006    17975172    23236062    22293439    30723633    21993624    25319552    17431167    22002653    26830274    20360741    16136131    20566863    23594743    25362486    22751099   

EMBL

NWSH01001943    PCG69615.1    AGBW02015069    OWR40595.1    KQ459605    KPI91954.1    + More
KQ461196    KPJ06347.1    GEZM01051535    JAV74839.1    GEZM01051533    JAV74841.1    KQ971321    EFA00011.2    KYB28740.1    NEVH01020333    PNF21813.1    PNF21812.1    KK852981    KDR12950.1    LJIG01002956    KRT83984.1    KZ288305    PBC28785.1    JH431429    NNAY01004010    OXU18499.1    JH431841    CM004472    OCT84338.1    BC079695    AAH79695.1    OCT84339.1    KQ434809    KZC06556.1    BC121290    AAI21291.1    AEYP01035253    AEYP01035254    KK940306    KFQ50194.1    GBYB01001917    JAG71684.1    AAMC01102941    AAMC01102942    AAMC01102943    AAMC01102944    AAMC01102945    AAMC01102946    AAMC01102947    GL193222    EFB21511.1    JW861845    AFO94362.1    BC123412    AAI23413.1    QUSF01000023    RLW01270.1    KL887256    KGL74715.1    KL669222    KFW75862.1    D42053    AK291773    AC040169    BC114555    BC114961    AL133583    AAEX03004026    AAEX03004027    GEBF01001246    JAO02387.1    KE161800    EPQ05449.1    KB743829    EOA96927.1    KL870139    KGL86881.1    AANG04002512    JP013074    AES01672.1    AKHW03005795    KYO25431.1    AGTP01083736    AGTP01083737    AGTP01083738    ADFV01071441    ADFV01071442    ADFV01071443    AEMK02000039    DQIR01157021    DQIR01213789    DQIR01298853    HDB12498.1    AAKN02054945    AQIA01043817    AQIA01043818    AQIA01043819    AQIA01043820    AQIB01129880    AHZZ02019146    AHZZ02019147    JU477639    AFH34443.1    JU336898    JV048573    AFE80651.1    AFI38644.1    JSUE03026761    JSUE03026762    CM001272    EHH31903.1    GEDV01011589    JAP76968.1    KL447421    KFO73387.1    ABQF01035918    AACZ04051371    AC195663    GABC01003307    GABF01005018    GABD01002378    GABE01010437    NBAG03000295    JAA08031.1    JAA17127.1    JAA30722.1    JAA34302.1    PNI45721.1    DS235430    EEB15675.1    KZ505864    PKU43898.1    KL378584    KFP85686.1    BX548157    CT025908    CAN88262.1    KX013132    KX013134    KX013138    KX013139    KX013140    KX013142    KX013143    KX013144    APA62631.1    APA62633.1    APA62637.1    APA62638.1    APA62639.1    APA62641.1    APA62642.1    APA62643.1    GEDV01008281    JAP80276.1    KL410965    KFR01634.1    KL343431    KFZ54330.1    KX013135    KX013136    APA62634.1    APA62635.1    KK735695    KFR15332.1    LSYS01005191    OPJ78140.1    KK824125    KFP71799.1    KK448607    KFQ72377.1    JH882006    ELR51402.1    CR861261    CAH93329.1   

Proteomes

UP000218220    UP000007151    UP000053268    UP000053240    UP000007266    UP000192223    + More
UP000235965    UP000027135    UP000242457    UP000005203    UP000215335    UP000186698    UP000085678    UP000076502    UP000000715    UP000248482    UP000261680    UP000291021    UP000008143    UP000248481    UP000261681    UP000001646    UP000252040    UP000009136    UP000248483    UP000276834    UP000053641    UP000053258    UP000248480    UP000265300    UP000005640    UP000002254    UP000053858    UP000011712    UP000050525    UP000005215    UP000001073    UP000008227    UP000233060    UP000245320    UP000189705    UP000005447    UP000233120    UP000233100    UP000029965    UP000028761    UP000233140    UP000006718    UP000053760    UP000007754    UP000002277    UP000009046    UP000000437    UP000233180    UP000233200    UP000233080    UP000053283    UP000007646    UP000053605    UP000190648    UP000233020   

PRIDE

Q14703   

Pfam

PF00082   Peptidase_S8

Interpro

IPR023828   Peptidase_S8_Ser-AS        + More
IPR036852   Peptidase_S8/S53_dom_sf       
IPR022398   Peptidase_S8_His-AS       
IPR015500   Peptidase_S8_subtilisin-rel       
IPR000209   Peptidase_S8/S53_dom       
IPR034185   Site-1_peptidase_cat_dom       

SUPFAM

SSF52743   SSF52743       

Gene 3D

3.40.50.200    

CDD

cd07479  Peptidases_S8_SKI-1_like

ProteinModelPortal

A0A2A4JC15   A0A212EGG8   A0A194PGX1   A0A194QLD6   A0A1Y1LMH9   A0A1Y1LPH7   + More
D6WIB2   A0A139WLB7   A0A1W4WJX8   A0A1W4WIY9   A0A2J7PZP8   A0A2J7PZR5   A0A067QSV2   A0A0T6B9G1   A0A2A3EAP1   T1ISC2   A0A088A5Z3   A0A232EJF7   T1J4A8   A0A1L8GKG1   Q6AX98   A0A1L8GKI6   A0A1S3HYQ9   A0A154P3R4   Q0VA23   M3YC21   A0A2Y9K244   A0A384BZG3   A0A091S4F9   A0A0C9QMP8   F6S2F2   D2HRI4   A0A2Y9IG02   V9K9C3   A0A2Y9SY48   A0A384A5I7   G1KN46   A0A341AP56   Q08E55   A0A2Y9P8W9   A0A3L8SFL4   A0A2Y9SPV3   A0A2Y9NXL1   A0A099Z1P2   A0A093PUW3   A0A2Y9DNI6   A0A340X0E6   A0A2Y9K2N3   Q14703   F1PER6   A0A0P6JL18   S7MMG9   R0L9K5   A0A099ZYJ0   M3WFB1   G9K9U7   A0A151MLJ3   I3MBB7   G1RJD6   A0A286ZZH4   A0A2K5KZ34   A0A2U4BRP0   A0A1U7RFK3   A0A1U7R8F2   H0V6Z3   A0A2K6CMT2   A0A2K5V1G2   A0A2K5V1H8   A0A0D9S1D8   A0A2U4BRQ6   A0A096NPH3   H9ZES7   A0A2K5Z4Q0   H9G192   F7G9K2   A0A131YEJ8   A0A091GGY9   H0Z8S1   H2QBL9   E0VQL9   A0A2I0UCW7   A0A091N8D2   Q1LWH3   A0A1I9XGB5   A0A2K6K2W1   A0A2K6R346   A0A2K5IJ30   A0A131YQA9   A0A091VER6   A0A094KTH6   A0A1I9XGB3   G3SXF6   A0A091WI36   A0A1V4K102   A0A091N6X0   A0A091TEW2   L8I827   Q5R4I7   A0A2K5F5D8  

PDB

1BH6     E-value=2.55657e-20,     Score=247

KEGG

101735340    K08653 membrane-bound transcription factor site-1 protease [EC:3.4.21.112] | (RefSeq) membrane-bound

PATHWAY

04141    Protein processing in endoplasmic reticulum - Bombyx mori (domestic silkworm)

GO

GO:0004252   GO:0016021   GO:0005794   GO:0006606   GO:0007040   GO:0005795   GO:0006629   GO:0001889   GO:0051216   GO:0044267   GO:0034976   GO:0000139   GO:0005789   GO:0030968   GO:0008203   GO:0031293   GO:0043687   GO:0005788   GO:0045540   GO:0036500   GO:0006508   GO:0004175   GO:0004190   GO:0043565   GO:0043401   GO:0003677   GO:0003707   GO:0007169  

Subcellular location

Endoplasmic reticulum membrane  
Golgi apparatus membrane  

SignalP

Position:   1 - 22,         Likelihood:  0.974286

 

 

Length:

1042

Number of predicted TMHs:

1

Exp number of AAs in TMHs:

19.1747

Exp number, first 60 AAs:

0.90125

Total prob of N-in:

0.04737

outside

1  -  1007

TMhelix

1008  -  1025

inside

1026  -  1042

 

 

Pi

3.951263

Theta

16.687174

Tajima's D

-2.184838

CLR

0.032736

CSRT

0.00614969251537423

Interpretation

Uncertain